[ Objective] To study the growth-development of Boer goats from embryo transplant. [ Method] With Boer goats from embryo transplant as the tested animals and natural breeding Boer goats born in the same period as CK, ...[ Objective] To study the growth-development of Boer goats from embryo transplant. [ Method] With Boer goats from embryo transplant as the tested animals and natural breeding Boer goats born in the same period as CK, the weight of Boer goats at birth and at the age of 1,3 and 6 months as well as the body size at the age of 3 and 6 months were investigated in this study, while their health conditions were also observed and recorded. [ Result] Under the normal feeding conditions, Boer goats from embryo transplant developed well at early stage, and their meat-using body type were also obvious. The average birth weight of bucks was (4.25±0.95) kg, and that of does was (3.74±0.10) kg. The average weight of bucks at the age of 6 months was (31.90 ±0.74) kg and that of was (25.90 ±0.67) kg. The daily gains of bucks and ewes from birth to 6 months of age were 153.64 and 123.11 g respectively, while their body length, body height and chest circumference increased obviously, and there was no significant difference in body weight or body size between them and natural breeding goats. [ Condusioa] The study lays a foundation for further studies on the breedinq and utilization of Boer noats from embryo transplant at early staae.展开更多
The early effects of chromosomal manipulation of eggs and sperm on the yields of triploid and gynogenetic diploid larvae of Paralichthys olivaceus were investigated. Triploidy was achieved by cold shocking fertilized ...The early effects of chromosomal manipulation of eggs and sperm on the yields of triploid and gynogenetic diploid larvae of Paralichthys olivaceus were investigated. Triploidy was achieved by cold shocking fertilized eggs at 0-2℃ for 45 minutes duration 5 minutes after fertilization, and the induced triploidy rates were 31.2%-50% and the relative hatching rates were 53.3%-99%. Gynogenetic diploids were obtained when eggs were inseminated with irradiated sperm and cold shocked at 0-2℃ for 45 minutes duration 5 minutes after fertilization. The induced gynogenetic diploid rates and the relative hatching rates were 94%-96% and 48.5%-68.5% respectively. The embryonic development of the triploid experimental group and of the gynogenetic diploid experimental group was delayed at first compared with the control group. But from the gastrula stage, it was not delayed anymore. There were no significant differences in the growth of the triploid experimental group larvae and the control group larvae, and in the growth of the gynogenetic diploid experimental group larvae and the control group larvae according to Student’s t test (α=0.05). The relationship between the early growth of the triploid experimental group larvae and that of gynogenetic diploid experimental group larvae was also studied.展开更多
Background: Zearalenone (ZEN) is an estrogenic mycotoxin that is primarily produced by Fusarium fungi and has been proven to affect the reproductive capacity of many species to varying degrees. The present experime...Background: Zearalenone (ZEN) is an estrogenic mycotoxin that is primarily produced by Fusarium fungi and has been proven to affect the reproductive capacity of many species to varying degrees. The present experiment was designed to study the maternal persistent effects of zearalenone toxicity in gestating sows on growth and muscle development of their offsprings, and the alleviation of zearalenone toxicity by modified halloysite nanotubes (MHNTs). Methods: Eighteen sows were fed with one of three dietary treatments that included the following: (1) a control diet, (2) a contaminated grain diet (with 50 % moldy corn, 2.77 mg/kg ZEN), and (3) a contaminated grain diet (with 50 % moldy corn, 2.76 mg/kg ZEN) + 1% MHNTs. Each sow was exclusively fed its experimental diets from 35 to 70 d of gestation at a total of 2 kg daily. Muscle samples were collected from six piglets per treatment at birth, weaning and finishing. Results: The results showed that feeding the sows with the ZEN-contaminated diets from 35 to 70 d of gestation decreased the ADG, ADFI and G:F of their offsprings (P 〈 0.05). The muscle fiber numbers in the newborn, weaning and growing-finishing pigs and the muscle fiber diameters at birth and weaning were also decreased by maternal ZEN exposure (P 〈 0.05). The expressions of IGF-I, IGF-II, Myf-5 and Mstn at birth and IGF-II, Pax7, Myf-5 and MyoD1 at weaning were altered by feeding gestating sows with ZEN-contaminated diets (P 〈 0.05). The MHNTs reduced most of the ZEN-induced toxic effects: the ADG and ADFI on growth performance, the muscle fiber numbers at weaning and finishing and the muscle fiber diameters at weaning (P 〈 0.05). The expression levels of IGF-II and Mstn in newborn piglets and IGF-II and Myf-5 in weaning piglets were also prevented by adding 1% MHNTs (P 〈 0.05). Conclusions: The present study demonstrated that the offsprings of sows fed with ZEN-contaminated diets from 35 to 70 day of gestation exhibited weakening on growth performance, physiological changes in their muscle fibers and alterations of mRNA expression in their muscle tissues, and also indicated that MHNTs prevented most of the ZEN- induced weakening in the muscle tissues.展开更多
Introduction:Early childhood development(ECD)centres are important community hubs in South Africa and act as sites for community detection of childhood nutrition problems.This study aimed to assess the ability of trai...Introduction:Early childhood development(ECD)centres are important community hubs in South Africa and act as sites for community detection of childhood nutrition problems.This study aimed to assess the ability of trained ECD practitioners with optimal support to correctly classify the nutritional status of infants and young children at ECD centres in the Nelson Mandela Bay.Methods:A descriptive,cross-sectional study was used to collect data from 1645 infants and children at 88 ECD centres.Anthropometric measurements were taken by trained fieldworkers and growth monitoring and promotion infrastructure was audited at ECD centres.Results:Of the sample,4.4%(n=72)were underweight by weight for age Z-score(WAZ<-2)and 0.8%(n=13)were severely underweight(WAZ<-3).Results showed that 13.1%(n=214)were stunted by height for age Z-score(HAZ<-2)and 4.5%(n=74)were severely stunted(HAZ<-3).The prevalence of moderate acute malnutrition was 1.2%and severe acute malnutrition was 0.5%,while the prevalence of overweight was 9.2%and the prevalence of obesity was 4%.A significant level of agreement between the correct interpretation and the ECD practitioners'interpretation was observed across all the anthropometric indicators investigated.The true positive wasting cases had a mean mid-upper arm circumference(MUAC)of 14.6 cm,which may explain the high false negative rate found in terms of children identified with wasting,where ECD practitioners fail to use the weight for height Z-score(WHZ)interpretation for screening.Conclusion:By using ECD centres as hub to screen for malnutrition,it may contribute to the early identification of failure to thrive among young children.Although it was concerning that trained ECD practitioners are missing some children with an unacceptably high false negative rate,it may have been due to the fact that wasting in older children cannot be identified with MUAC alone and that accurate WFH plotting is needed.Onsite mentorship by governmental health workers may provide ECD practitioners with more confidence to screen children for growth failure based on regular WFH measurements.Moreover,ECD practitioners will be more confident to monitor the Road to Health booklets for missed vaccinations,vitamin A and deworming opportunities.展开更多
Background: Most research on galacto-oligosaccharides(GOS) has mainly focused on their prebiotic effects on the hindgut,but their beneficial effects on the small intestine(SI) have received little attention.Since jeju...Background: Most research on galacto-oligosaccharides(GOS) has mainly focused on their prebiotic effects on the hindgut,but their beneficial effects on the small intestine(SI) have received little attention.Since jejunum is the important place to digest and absorb nutrients efficiently,optimal maturation of the jejunum is necessary for maintaining the high growth rate in the neonate.Therefore,this study investigates the effect of the early intervention with GOS on the intestinal development of the jejunum.Methods: A total of 6 litters of neonatal piglets(10 piglets per litter; Duroc × Landrace × Large White) with an average birth weight of 1.55 ± 0.05 kg received 1 of 2 treatments based on their assignment to either the control(CON) group or the GOS(GOS) group in each litter.Piglets in the GOS group were orally administrated 10 mL of a GOS solution(reaching 1 g GOS/kg body weight) per day from the age of 1 to 7 d; the piglets in the CON group were treated with the same dose of physiological saline.All piglets were weaned on d 21.On d 8 and 21 of the experimental trial,1 pig per group from each of the 6 litters was euthanized.Results: The early intervention with GOS increased the average daily gains in the third week(P < 0.05).Decreased crypt depth was also observed in the jejunum of the piglets on d 21(P < 0.05).The early intervention with GOS increased the jejunal lactase activity on d 8,maltase activity and sucrase activity on d 21(P < 0.05).In addition,the early intervention with GOS also facilitated the mRNA expression of Sodium glucose co-transporter 1(SGLT1) on d 8 and the m RNA expression of Glucose transporter type 2(GLUT2) on d 21(P < 0.05).It was further determined that GOS up-regulated the m RNA expression of preproglucagon(GCG),insulin-like growth factor 1(IGF-1),insulin-like growth factor 1 receptor(IGF-1 R) and epidermal growth factor(EGF).GOS also up-regulated the protein expression of glucagon-like peptide-2(GLP-2) and EGF in the jejunum of the piglets.Furthermore,it was also found that GOS enhanced the protein expression of ZO-1 and occludin on d 8(P < 0.05),as well as increased the mRNA expression of TGF-β and decrease the mRNA expression of IL-12(P < 0.05).Conclusions: These results indicate that GOS have a positive effect on piglet growth performance in addition to decreasing the crypt depth and enhancing functional development in jejunum of suckling piglets.展开更多
Background: Intra-uterine crowding(IUC) observed in hyperprolific sows impairs myofiber hyperplasia and overall fetal growth. Arginine supplementation(ARG) in gestation diets has been shown to positively affect litter...Background: Intra-uterine crowding(IUC) observed in hyperprolific sows impairs myofiber hyperplasia and overall fetal growth. Arginine supplementation(ARG) in gestation diets has been shown to positively affect litter and muscle development. The study objective was to assess whether the effect of ARG on offspring characteristics, with special emphasis on myofiber hyperplasia, differs under IUC conditions from these responses,because in that situation growth retardation is particularly prevalent due to reduced fetal nutrient supply.Unilateral oviduct ligation(OL) was used as a model for an uncrowded and hyperprolificacy(IN) as a model for a crowded intra-uterine environment.Methods: Five OL and five IN sows were fed a diet supplemented daily with either 43 g L-alanine(Ctrl) or 25 g L-arginine from d 14 to 28 of gestation in a cross-over design(two periods: 5^( th) and 6^( th) parity). At farrowing, two male and two female offspring, respectively, with a low and intermediate birth weight(BtW) were selected. After euthanization, the Semitendinosus muscle(STM) was removed and weighed, and the light and dark portions(STM_d and STM_l) were prepared for myofiber histochemistry using ATPase staining and the entire STM for gene expression analysis of myogenesis-related genes using RT-qPCR. In addition, various organs were removed and weighed. Data were analyzed using the MIXED model in SYSTAT.Results: No effect of either IUC or dietary treatment was found in litter characteristics. Offspring of ARG sows displayed a greater muscle area in STM(P < 0.01) as a result of the greater myofiber hyperplasia(P < 0.01). The increase was more distinct in the STM_l(P < 0.05) than in the STM_d(P = 0.131). Offspring of OL sows were heavier at birth(P < 0.01), had a heavier STM(P < 0.05), liver(P < 0.01) and kidney(P < 0.05), but when expressed relative to birth weight, these differences were absent. In addition, IUC had an effect(P < 0.05) on the expression of one of the myogenesis-related genes investigated.Conclusions: Independent from the extent of IUC, ARG improved Bt W, muscle and organ weights and myofiber hyperplasia in offspring.展开更多
基金supported by Talent Research Foundation of Henan University of Technology (09001095)Scientific and Technological Project of Henan Province " Research and Demonstration of Supporting Production Technology Systemfor High-quality Funiu White Goats" (072102130004)
文摘[ Objective] To study the growth-development of Boer goats from embryo transplant. [ Method] With Boer goats from embryo transplant as the tested animals and natural breeding Boer goats born in the same period as CK, the weight of Boer goats at birth and at the age of 1,3 and 6 months as well as the body size at the age of 3 and 6 months were investigated in this study, while their health conditions were also observed and recorded. [ Result] Under the normal feeding conditions, Boer goats from embryo transplant developed well at early stage, and their meat-using body type were also obvious. The average birth weight of bucks was (4.25±0.95) kg, and that of does was (3.74±0.10) kg. The average weight of bucks at the age of 6 months was (31.90 ±0.74) kg and that of was (25.90 ±0.67) kg. The daily gains of bucks and ewes from birth to 6 months of age were 153.64 and 123.11 g respectively, while their body length, body height and chest circumference increased obviously, and there was no significant difference in body weight or body size between them and natural breeding goats. [ Condusioa] The study lays a foundation for further studies on the breedinq and utilization of Boer noats from embryo transplant at early staae.
文摘The early effects of chromosomal manipulation of eggs and sperm on the yields of triploid and gynogenetic diploid larvae of Paralichthys olivaceus were investigated. Triploidy was achieved by cold shocking fertilized eggs at 0-2℃ for 45 minutes duration 5 minutes after fertilization, and the induced triploidy rates were 31.2%-50% and the relative hatching rates were 53.3%-99%. Gynogenetic diploids were obtained when eggs were inseminated with irradiated sperm and cold shocked at 0-2℃ for 45 minutes duration 5 minutes after fertilization. The induced gynogenetic diploid rates and the relative hatching rates were 94%-96% and 48.5%-68.5% respectively. The embryonic development of the triploid experimental group and of the gynogenetic diploid experimental group was delayed at first compared with the control group. But from the gastrula stage, it was not delayed anymore. There were no significant differences in the growth of the triploid experimental group larvae and the control group larvae, and in the growth of the gynogenetic diploid experimental group larvae and the control group larvae according to Student’s t test (α=0.05). The relationship between the early growth of the triploid experimental group larvae and that of gynogenetic diploid experimental group larvae was also studied.
基金supported by National Basic Research Program (2012CB124703)the China Agriculture Research System (CARS-36)Program for Innovative Research Team of Universities in Heilongjiang Province (2012TD003)
文摘Background: Zearalenone (ZEN) is an estrogenic mycotoxin that is primarily produced by Fusarium fungi and has been proven to affect the reproductive capacity of many species to varying degrees. The present experiment was designed to study the maternal persistent effects of zearalenone toxicity in gestating sows on growth and muscle development of their offsprings, and the alleviation of zearalenone toxicity by modified halloysite nanotubes (MHNTs). Methods: Eighteen sows were fed with one of three dietary treatments that included the following: (1) a control diet, (2) a contaminated grain diet (with 50 % moldy corn, 2.77 mg/kg ZEN), and (3) a contaminated grain diet (with 50 % moldy corn, 2.76 mg/kg ZEN) + 1% MHNTs. Each sow was exclusively fed its experimental diets from 35 to 70 d of gestation at a total of 2 kg daily. Muscle samples were collected from six piglets per treatment at birth, weaning and finishing. Results: The results showed that feeding the sows with the ZEN-contaminated diets from 35 to 70 d of gestation decreased the ADG, ADFI and G:F of their offsprings (P 〈 0.05). The muscle fiber numbers in the newborn, weaning and growing-finishing pigs and the muscle fiber diameters at birth and weaning were also decreased by maternal ZEN exposure (P 〈 0.05). The expressions of IGF-I, IGF-II, Myf-5 and Mstn at birth and IGF-II, Pax7, Myf-5 and MyoD1 at weaning were altered by feeding gestating sows with ZEN-contaminated diets (P 〈 0.05). The MHNTs reduced most of the ZEN-induced toxic effects: the ADG and ADFI on growth performance, the muscle fiber numbers at weaning and finishing and the muscle fiber diameters at weaning (P 〈 0.05). The expression levels of IGF-II and Mstn in newborn piglets and IGF-II and Myf-5 in weaning piglets were also prevented by adding 1% MHNTs (P 〈 0.05). Conclusions: The present study demonstrated that the offsprings of sows fed with ZEN-contaminated diets from 35 to 70 day of gestation exhibited weakening on growth performance, physiological changes in their muscle fibers and alterations of mRNA expression in their muscle tissues, and also indicated that MHNTs prevented most of the ZEN- induced weakening in the muscle tissues.
基金Nelson Mandela University,London Metropolitan University and UNICEF。
文摘Introduction:Early childhood development(ECD)centres are important community hubs in South Africa and act as sites for community detection of childhood nutrition problems.This study aimed to assess the ability of trained ECD practitioners with optimal support to correctly classify the nutritional status of infants and young children at ECD centres in the Nelson Mandela Bay.Methods:A descriptive,cross-sectional study was used to collect data from 1645 infants and children at 88 ECD centres.Anthropometric measurements were taken by trained fieldworkers and growth monitoring and promotion infrastructure was audited at ECD centres.Results:Of the sample,4.4%(n=72)were underweight by weight for age Z-score(WAZ<-2)and 0.8%(n=13)were severely underweight(WAZ<-3).Results showed that 13.1%(n=214)were stunted by height for age Z-score(HAZ<-2)and 4.5%(n=74)were severely stunted(HAZ<-3).The prevalence of moderate acute malnutrition was 1.2%and severe acute malnutrition was 0.5%,while the prevalence of overweight was 9.2%and the prevalence of obesity was 4%.A significant level of agreement between the correct interpretation and the ECD practitioners'interpretation was observed across all the anthropometric indicators investigated.The true positive wasting cases had a mean mid-upper arm circumference(MUAC)of 14.6 cm,which may explain the high false negative rate found in terms of children identified with wasting,where ECD practitioners fail to use the weight for height Z-score(WHZ)interpretation for screening.Conclusion:By using ECD centres as hub to screen for malnutrition,it may contribute to the early identification of failure to thrive among young children.Although it was concerning that trained ECD practitioners are missing some children with an unacceptably high false negative rate,it may have been due to the fact that wasting in older children cannot be identified with MUAC alone and that accurate WFH plotting is needed.Onsite mentorship by governmental health workers may provide ECD practitioners with more confidence to screen children for growth failure based on regular WFH measurements.Moreover,ECD practitioners will be more confident to monitor the Road to Health booklets for missed vaccinations,vitamin A and deworming opportunities.
基金supported by the National Key R&D Program of China2017YFD0500505the Fundamental Research Funds for the Central Universities,China(KYZ201722)
文摘Background: Most research on galacto-oligosaccharides(GOS) has mainly focused on their prebiotic effects on the hindgut,but their beneficial effects on the small intestine(SI) have received little attention.Since jejunum is the important place to digest and absorb nutrients efficiently,optimal maturation of the jejunum is necessary for maintaining the high growth rate in the neonate.Therefore,this study investigates the effect of the early intervention with GOS on the intestinal development of the jejunum.Methods: A total of 6 litters of neonatal piglets(10 piglets per litter; Duroc × Landrace × Large White) with an average birth weight of 1.55 ± 0.05 kg received 1 of 2 treatments based on their assignment to either the control(CON) group or the GOS(GOS) group in each litter.Piglets in the GOS group were orally administrated 10 mL of a GOS solution(reaching 1 g GOS/kg body weight) per day from the age of 1 to 7 d; the piglets in the CON group were treated with the same dose of physiological saline.All piglets were weaned on d 21.On d 8 and 21 of the experimental trial,1 pig per group from each of the 6 litters was euthanized.Results: The early intervention with GOS increased the average daily gains in the third week(P < 0.05).Decreased crypt depth was also observed in the jejunum of the piglets on d 21(P < 0.05).The early intervention with GOS increased the jejunal lactase activity on d 8,maltase activity and sucrase activity on d 21(P < 0.05).In addition,the early intervention with GOS also facilitated the mRNA expression of Sodium glucose co-transporter 1(SGLT1) on d 8 and the m RNA expression of Glucose transporter type 2(GLUT2) on d 21(P < 0.05).It was further determined that GOS up-regulated the m RNA expression of preproglucagon(GCG),insulin-like growth factor 1(IGF-1),insulin-like growth factor 1 receptor(IGF-1 R) and epidermal growth factor(EGF).GOS also up-regulated the protein expression of glucagon-like peptide-2(GLP-2) and EGF in the jejunum of the piglets.Furthermore,it was also found that GOS enhanced the protein expression of ZO-1 and occludin on d 8(P < 0.05),as well as increased the mRNA expression of TGF-β and decrease the mRNA expression of IL-12(P < 0.05).Conclusions: These results indicate that GOS have a positive effect on piglet growth performance in addition to decreasing the crypt depth and enhancing functional development in jejunum of suckling piglets.
文摘Background: Intra-uterine crowding(IUC) observed in hyperprolific sows impairs myofiber hyperplasia and overall fetal growth. Arginine supplementation(ARG) in gestation diets has been shown to positively affect litter and muscle development. The study objective was to assess whether the effect of ARG on offspring characteristics, with special emphasis on myofiber hyperplasia, differs under IUC conditions from these responses,because in that situation growth retardation is particularly prevalent due to reduced fetal nutrient supply.Unilateral oviduct ligation(OL) was used as a model for an uncrowded and hyperprolificacy(IN) as a model for a crowded intra-uterine environment.Methods: Five OL and five IN sows were fed a diet supplemented daily with either 43 g L-alanine(Ctrl) or 25 g L-arginine from d 14 to 28 of gestation in a cross-over design(two periods: 5^( th) and 6^( th) parity). At farrowing, two male and two female offspring, respectively, with a low and intermediate birth weight(BtW) were selected. After euthanization, the Semitendinosus muscle(STM) was removed and weighed, and the light and dark portions(STM_d and STM_l) were prepared for myofiber histochemistry using ATPase staining and the entire STM for gene expression analysis of myogenesis-related genes using RT-qPCR. In addition, various organs were removed and weighed. Data were analyzed using the MIXED model in SYSTAT.Results: No effect of either IUC or dietary treatment was found in litter characteristics. Offspring of ARG sows displayed a greater muscle area in STM(P < 0.01) as a result of the greater myofiber hyperplasia(P < 0.01). The increase was more distinct in the STM_l(P < 0.05) than in the STM_d(P = 0.131). Offspring of OL sows were heavier at birth(P < 0.01), had a heavier STM(P < 0.05), liver(P < 0.01) and kidney(P < 0.05), but when expressed relative to birth weight, these differences were absent. In addition, IUC had an effect(P < 0.05) on the expression of one of the myogenesis-related genes investigated.Conclusions: Independent from the extent of IUC, ARG improved Bt W, muscle and organ weights and myofiber hyperplasia in offspring.