Objective:To explore the genetic variation and differentiation of 2 echinostomes from genus Echinostoma,i.e.Echinostoma revolutum(E.revolution) and Echinostoma malayanum(E. malayanum) from Khon Kaen Province,Thailand....Objective:To explore the genetic variation and differentiation of 2 echinostomes from genus Echinostoma,i.e.Echinostoma revolutum(E.revolution) and Echinostoma malayanum(E. malayanum) from Khon Kaen Province,Thailand.Methods:These parasites were compared at 22 enzymes encoding a presumptive 30 loci by using multilocus enzyme electrophoresis (MEE) technique.Results:Twenty-two loci can be used as diagnostic markers to differentiate these 2 species.E.revolutum and E.malayanum had fixed genetic differences at 70%of loci, whereas both species had fixed genetic differences from the liver fluke,Opisthorchis viverrini at 91%of loci.Intraspecific variation within a population of E.revolutum was observed at 5 polymorphic loci.Conclusions:MEE is a powerful technique to investigate genetic variation and differentiation of E.revolutum and E.malayanum.展开更多
Objective:To analyze the prevalence of echinostome metacercariae in Filopaludina dorliaris(F.dorliaris)and Filopaludina martensi martensi(F.martensi martensi)and genotype variation of echinostome metacercariae by usin...Objective:To analyze the prevalence of echinostome metacercariae in Filopaludina dorliaris(F.dorliaris)and Filopaludina martensi martensi(F.martensi martensi)and genotype variation of echinostome metacercariae by using random amplified polymorphic DNA(RAPD)analysis.Methods:Filopaludina sp.snails were collected from eight localities of Lamphun Province,Northern Thailand and examined for echinostome metacercariae.RAPD-PCR was used lo analyze genotype variation of echinostome metacercariae.Results:A total of 3 226 F.dorliaris and F.martensi martensi snails were collected from eight localities.The overall prevalences of echinostome metacercariae in F.dorliaris and F.martensi martensi were 40.89%and 36.27%,while the intensity of infection was 20.37 and 12.04,respectively.The dendrogram constructed base on RAPD profiles,4 well supported domains were generated;(i)group of metacercariae from Ban Hong,Mae Ta,Meaung,Pa Sang,Toong Hua Chang,and Weang Nong that were clustered in the group of E.revolutum,(ii)Ban Thi,(iii)Lee,and(iv)3 adults of an out group.Condusioiis:This research demonstrated RAPD profiling has been a useful tool to detect DNA polymorphisms to determine genetic relationship between echinostome metacercariae in Lamphun Province,Northern Thailand.展开更多
基金supported by the Thailand Research Fund and the Commission on Higher Education(grant no. MRG5180102 to Weerachai Saijuntha).
文摘Objective:To explore the genetic variation and differentiation of 2 echinostomes from genus Echinostoma,i.e.Echinostoma revolutum(E.revolution) and Echinostoma malayanum(E. malayanum) from Khon Kaen Province,Thailand.Methods:These parasites were compared at 22 enzymes encoding a presumptive 30 loci by using multilocus enzyme electrophoresis (MEE) technique.Results:Twenty-two loci can be used as diagnostic markers to differentiate these 2 species.E.revolutum and E.malayanum had fixed genetic differences at 70%of loci, whereas both species had fixed genetic differences from the liver fluke,Opisthorchis viverrini at 91%of loci.Intraspecific variation within a population of E.revolutum was observed at 5 polymorphic loci.Conclusions:MEE is a powerful technique to investigate genetic variation and differentiation of E.revolutum and E.malayanum.
基金supported by the Ministry of Science and Technology and the Graduate School,Chiang Mai Univeraity
文摘Objective:To analyze the prevalence of echinostome metacercariae in Filopaludina dorliaris(F.dorliaris)and Filopaludina martensi martensi(F.martensi martensi)and genotype variation of echinostome metacercariae by using random amplified polymorphic DNA(RAPD)analysis.Methods:Filopaludina sp.snails were collected from eight localities of Lamphun Province,Northern Thailand and examined for echinostome metacercariae.RAPD-PCR was used lo analyze genotype variation of echinostome metacercariae.Results:A total of 3 226 F.dorliaris and F.martensi martensi snails were collected from eight localities.The overall prevalences of echinostome metacercariae in F.dorliaris and F.martensi martensi were 40.89%and 36.27%,while the intensity of infection was 20.37 and 12.04,respectively.The dendrogram constructed base on RAPD profiles,4 well supported domains were generated;(i)group of metacercariae from Ban Hong,Mae Ta,Meaung,Pa Sang,Toong Hua Chang,and Weang Nong that were clustered in the group of E.revolutum,(ii)Ban Thi,(iii)Lee,and(iv)3 adults of an out group.Condusioiis:This research demonstrated RAPD profiling has been a useful tool to detect DNA polymorphisms to determine genetic relationship between echinostome metacercariae in Lamphun Province,Northern Thailand.
