Spatial-temporal changes and driving factors of eco-environmental quality in the Three-North region of China

Eco-environmental quality is a measure of the suitability of the ecological environment for human survival and socioeconomic development.Understanding the spatial-temporal distribution and variation trend of eco-environmental quality is essential for environmental protection and ecological balance.The remote sensing ecological index(RSEI)can quickly and objectively quantify eco-environmental quality and has been extensively utilized in regional ecological environment assessment.In this paper,Moderate Resolution Imaging Spectroradiometer(MODIS)images during the growing period(July-September)from 2000 to 2020 were obtained from the Google Earth Engine(GEE)platform to calculate the RSEI in the three northern regions of China(the Three-North region).The Theil-Sen median trend method combined with the Mann-Kendall test was used to analyze the spatial-temporal variation trend of eco-environmental quality,and the Hurst exponent and the Theil-Sen median trend were superimposed to predict the future evolution trend of eco-environmental quality.In addition,ten variables from two categories of natural and anthropogenic factors were analyzed to determine the drivers of the spatial differentiation of eco-environmental quality by the geographical detector.The results showed that from 2000 to 2020,the RSEI in the Three-North region exhibited obvious regional characteristics:the RSEI values in Northwest China were generally between 0.2 and 0.4;the RSEI values in North China gradually increased from north to south,ranging from 0.2 to 0.8;and the RSEI values in Northeast China were mostly above 0.6.The average RSEI value in the Three-North region increased at an average growth rate of 0.0016/a,showing the spatial distribution characteristics of overall improvement and local degradation in eco-environmental quality,of which the areas with improved,basically stable and degraded eco-environmental quality accounted for 65.39%,26.82%and 7.79%of the total study area,respectively.The Hurst exponent of the RSEI ranged from 0.20 to 0.76 and the future trend of eco-environmental quality was generally consistent with the trend over the past 21 years.However,the areas exhibiting an improvement trend in eco-environmental quality mainly had weak persistence,and there was a possibility of degradation in eco-environmental quality without strengthening ecological protection.Average relative humidity,accumulated precipitation and land use type were the dominant factors driving the spatial distribution of eco-environmental quality in the Three-North region,and two-factor interaction also had a greater influence on eco-environmental quality than single factors.The explanatory power of meteorological factors on the spatial distribution of eco-environmental quality was stronger than that of topographic factors.The effect of anthropogenic factors(such as population density and land use type)on eco-environmental quality gradually increased over time.This study can serve as a reference to protect the ecological environment in arid and semi-arid regions.

Land cover change and eco-environmental quality response of different geomorphic units on the Chinese Loess Plateau

Land cover in the Chinese Loess Plateau has undergone dramatic changes since the late 1980s.Revealing the trend in land cover change and eco-environmental quality response of different geomorphic units in this stage is a realistic requirement for promoting sustainable development of the Chinese Loess Plateau.Based on the data of geomorphic units and land cover in 1990,2000,2010 and 2018 of the Chinese Loess Plateau,we studied the trend of land cover change and eco-environmental quality response of different geomorphic units by using a significance index of land cover change,a proportion index of land cover change and an eco-environmental response model.The results indicated that from 1990 to 2018,the areas of forestland and construction land substantially increased,whereas those of cropland,grassland,wetland and unused land considerably decreased.Land cover change exhibited large geomorphic differences,and the main conversion of land cover was from cropland into other land types.Unstable trend of land cover change in the loess tablelands and sandy loess hills declined,whereas the unstable trends in the other geomorphic units enhanced.Eco-environmental quality varied among different geomorphic units.The expansion of construction land and degradation of forestland,grassland and wetland resulted in the deterioration of eco-environmental quality.The conversion of cropland and unused land into forestland and grassland,and the conversion of grassland into forestland were the main factors that drove the improvement of eco-environmental quality.The findings of this study may provide theoretical reference and support decision making for the optimization of land use structure and the improvement of eco-environmental quality on the Chinese Loess Plateau.

Spatiotemporal changes of eco-environmental quality based on remote sensing-based ecological index in the Hotan Oasis,Xinjiang

The rapid economic development that the Hotan Oasis in Xinjiang Uygur Autonomous Region,China has undergone in recent years may face some challenges in its ecological environment.Therefore,an analysis of the spatiotemporal changes in ecological environment of the Hotan Oasis is important for its sustainable development.First,we constructed an improved remote sensing-based ecological index(RSEI)in 1990,1995,2000,2005,2010,2015 and 2020 on the Google Earth Engine(GEE)platform and implemented change detection for their spatial distribution.Second,we performed a spatial autocorrelation analysis on RSEI distribution map and used land-use and land-cover change(LUCC)data to analyze the reasons of RSEI changes.Finally,we investigated the applicability of improved RSEI to arid area.The results showed that mean of RSEI rose from 0.41 to 0.50,showing a slight upward trend.During the 30-a period,2.66% of the regions improved significantly,10.74% improved moderately and 32.21% improved slightly,respectively.The global Moran's I were 0.891,0.889,0.847 and 0.777 for 1990,2000,2010 and 2020,respectively,and the local indicators of spatial autocorrelation(LISA)distribution map showed that the high-high cluster was mainly distributed in the central part of the Hotan Oasis,and the low-low cluster was mainly distributed in the outer edge of the oasis.RSEI at the periphery of the oasis changes from low to high with time,with the fragmentation of RSEI distribution within the oasis increasing.Its distribution and changes are predominantly driven by anthropologic factors,including the expansion of artificial oasis into the desert,the replacement of desert ecosystems by farmland ecosystems,and the increase in the distribution of impervious surfaces.The improved RSEI can reflect the eco-environmental quality effectively of the oasis in arid area with relatively high applicability.The high efficiency exhibited with this approach makes it convenient for rapid,high frequency and macroscopic monitoring of eco-environmental quality in study area.

Universal Index Formula of Parameterized Combination Operator for Eco-environmental Quality Evaluation

[Objective] The aim was to evaluate the regional eco-environmental quality by using the universal index formula of parameterization combination operator based normalized index values.[Method] Through setting reference values and normalized transformation formulae for typical ecological environmental indexes appropriately,the difference among the standard normalized values would become very small after normalized transformation,and the ecological environmental indices expressed by normalized values can be equivalent to normalized indices.Under certain optimization conditions,shuffled frog leaping based on immune evolutionary particle swarm optimization algorithm was applied to optimize the parameters in parameterization combination operator formula,and the universal index formula suited to eco-environmental quality assessment was established finally.[Result] The universal index formula of parameterization combination operator,appropriate for any m(1≤m≤23) ecological environmental indices,was used to assess the eco-environmental quality of towns surrounding Headland Reservoir,and the results were in full accordance with those of unascertained measure method,that is,the eco-environmental quality of five towns around Headland Reservoir was the fourth grade.[Conclusion]The universal index formula of parameterization combination operator,suited to eco-environmental quality evaluation,is simple and intuitive in form,easy in computation and universal in application.

Grey Clustering Evaluation on Regional Eco-environmental Quality Based on Normalized Index Value

[Objective] The aim was to assess regional eco-environmental quality by means of grey clustering method based on normalized index value. [Method] Eco-environmental quality in Chaohu basin was assessed by using grey clustering method based on normalized index value, and the evaluation results were compared with those of unascertained measure method to verify the feasibility of grey clustering method used to evaluate regional eco-environmental quality. [Result] In the grey clustering assessment method based on normalized index value, indices whose standard normalized values in the same grade were close to each other were classified into one class and had the same whitening function, which reduced the number of whitening functions. Grey clustering method based on normalized index value was used to assess eco-environmental quality in Chaohu basin, and the evaluation results were basically in accordance with those of unascertained measure method, namely eco-environmental quality in Hefei, Chaohu and Lu’an belonged to the third (pass), fourth (worse) and fifth grade (bad), except for one grade difference in overall basin, and the results showed that the method had practicality and could be applied to assess regional eco-environmental quality. [Conclusion] The study could provide theoretical foundation for the establishment of comprehensive management countermeasures of regional ecological environment.

Assessing the impacts of ecological-living-productive land changes on eco-environmental quality in Xining City on Qinghai-Tibet Plateau, China

The Ecological-living-productive land(ELPL)classification system was proposed in an effort to steer China's land pattern to an ecological-centered path,with the development model shifting from a single function into more integrated multifunction land use.The focus is coordinating the man-land contradictions and developing an intensive,efficient and sustainable land use policy in an increasingly tense relationship between humans and nature.Driven by socioeconomic change and rapid population growth,many cities are undergoing urban sprawl,which involves the consumption of cropland and ecological land and threatens the ecological balance.This paper aims to quantitatively analyze the critical effects of ELPL changes on eco-environmental quality according to land use classification based on leading function of ecology,living and production from 1990 to 2015 with a case study of Xining City.Also,four future land use scenarios were simulated for 2030 using the Future Land Use Simulation(FLUS)model that couples human and natural effects.Our results show a decrease in productive land(PL)and an increase in ecological land(EL)and living land(LL)in Xining City.Forestry ecological land(FEL)covered the top largest proportion;agriculture productive land(APL)showed the greatest reduction and urban and rural living land(U-RLL)presented a dramatic increase.The eco-environmental quality improved in 1990-2010,mainly affected by the conversion of APL to FEL and GEL.However,the encroachment of U-RLL into APL,other ecological land(OEL)and FEL was the main contributor to the decline in eco-environmental quality in 2010-2015 as well as the primary reason for the increase area of lower-quality.The Harmonious Development(HD)-Scenario,characterized by a rational allocation of LL and PL and a better eco-environment,would have implications for planning and monitoring future management of ELPL,and may represent a valuable reference for local policy-makers.

Integrated Evaluation Model for Eco-Environmental Quality in Mountainous Region Based on Remote Sensing and GIS

Based on Remote Sensing （RS）, Geographic Information System （GIS）, and combining Principal Component Analysis, this paper designed a numerical integrated evaluation model for mountain eco-environment on the base of grid scale. Using this model, we evaluated the mountain eco-environmental quality in a case study area-the upper reaches of Minjiang River, and achieved a good result, which accorded well with the real condition. The study indicates that, the integrated evaluation model is suitable for multi-layer spatial factor computation, effectively lowing man＇s subjective influence in the evaluation process; treating the whole river basin as a system, the model shows full respect to the circulation of material and energy, synthetically embodies the determining impact of such natural condition as water-heat and landform, as well as human interference in natural eco-system; the evaluation result not only clearly presents mountainous vertical distribution features of input factors, but also provides a scientific and reliable thought for quantitatively evaluating mountain eco-environment.

Study on the Change of Eco-Environmental Quality in Jiufeng Mountain Area Based on InVEST Model

Habitat is not only an important place for animals and plants to inhabit and reproduce, but also the basis of human survival and development. With the large-scale development of mineral resources, land use types have changed sharply, fragile habitats tend to collapse, and the future sustainable development has lost a stable carrier. Habitat quality is a comprehensive expression of the interaction of many factors such as land, biology and water resources for many years. Natural factors are the most direct factors affecting habitat quality, and human activities are the most intense factors. The habitat module of invest model was used to evaluate the changes and temporal and spatial characteristics of habitat quality in Jiufeng Mountain from 1990 to 2018. It is found that the habitat quality in Jiufeng Mountain area shows a downward trend, and the ecological environment is very fragile. The high value areas of eco-environmental quality are mainly distributed in mountainous and hilly areas with high vegetation coverage;The low value areas of eco-environmental quality are mainly distributed in industrial and mining land and gully areas. With the development of society and economy and the development of mining industry in Jiufengshan area, the area of construction land is also increasing.

Dynamic Assessment on Regional Eco-environmental Quality Using AHP-Statistics Model—A Case Study of Chaohu Lake Basin

An indicator system closely connected with eco-environment, in which indicator involves in such fields as society, economy, resources, environment and ecology, is first proposed for eco-environmental quality assessment. Then, a hierarchical model with four levels is established by virtue of these indicators and attributes. In the model, weights of indicators and attributes are determined by combining Delphi method with the Analytic Hierarchy Process (AHP) and a statistic method is used to eliminate the influences arising from the differences in dimension and magnitude of indicators. On these grounds, an AHP-statistics model is provided for regional eco-environmental quality assessment. As a case, such AHP-statistics model is utilized in the dynamic analysis of regional eco-environmental assessment in Chaohu Lake basin. Study results show that natural environmental quality in the watershed was in the declining state while social environmental quality was in a markedly improved situation from 1996, and the synthetic eco-environmental quality was gradually and slowly improved under the common influences of both natural and social environmental factors. Example of application testified the capacities of above methodology to evaluate the real and dynamic state of regional eco-environmental quality.

Multi-agent Blind Model and Its Application to Regional Eco-environmental Quality Assessment

Through denoting each expert as an agent and viewing a multiple criteria decision-making as a synthesis problem of aggregating experts’ ratings, a multi-agent blind model (MABM) is developed for regional eco- environmental quality assessment. In this model, the ratings of the evaluated object under an index, given by expert group, are first utilized to construct a series of blind numbers. In general, each index will correspond to different blind numbers. On the basis of aggregating index weights, the rank score in the form of a blind number is obtained for the evaluated object. Then, by means of calculating expected value of the above blind number, its rank score is further converted into a crisp value. By way of comparing the expected value with classification standards, eco-environmental quality of the evaluated sample could be identified successfully in the end. As a case, the MABM is used to evaluate the eco-environmental quality of Chaohu Lake basin. Study result shows that the MABM is a useful model for regional eco-environmental quality assessment.

Dynamics of the eco-environmental quality in response to land use changes in rapidly urbanizing areas: A case study of Wuhan, China from 2000 to 2018

The dramatic land use changes that occur in rapidly urbanized areas are important inducement to changes in the eco-environmental quality.Investigating urban land use changes and their eco-environmental quality responses can provide theoretical support and a decision-making basis for sustainable and high-quality development in rapidly urbanizing areas.Taking Wuhan,China,as the study area,this paper extracts land use information using Landsat satellite remote sensing images and a support vector machine classification.Based on this,a remote sensing-based ecological index evaluation model including humidity,greenness,dryness and heat is constructed to explore the changes in land use and their eco-environmental quality responses from 2000 to 2018.The results show that(1)the structure,extent and spatial layout of land use in Wuhan from 2000 to 2018 have undergone tremendous changes under rapid urbanization,and the change of construction land is the greatest among all land use types;(2)the overall quality of eco-environment in Wuhan continues to improve as the scale of the improved eco-environment areas is greater than that of the deteriorated areas.The direction and magnitude of the impact of each indicator on the eco-environmental quality are different;(3)the improvement or deterioration of eco-environmental quality is closely related to the changes of different land use types within the study area.The eco-environmental quality shows significant spatial heterogeneity,especially between the main urban areas and the suburban areas.This paper argues that reasonably adjusting the land use structure can serve to maintain or even improve the quality of the regional eco-environment.Finally,this study puts forward suggestions for the coordinated development of land use and the eco-environment in rapidly urbanizing areas.

Dynamic changes of rumen microbiota and serum metabolome revealed increases in meat quality and growth performances of sheep fed bio‑fermented rice straw

Background Providing high-quality roughage is crucial for improvement of ruminant production because it is an essential component of their feed.Our previous study showed that feeding bio-fermented rice straw(BF)improved the feed intake and weight gain of sheep.However,it remains unclear why feeding BF to sheep increased their feed intake and weight gain.Therefore,the purposes of this research were to investigate how the rumen micro-biota and serum metabolome are dynamically changing after feeding BF,as well as how their changes influence the feed intake,digestibility,nutrient transport,meat quality and growth performances of sheep.Twelve growing Hu sheep were allocated into 3 groups:alfalfa hay fed group(AH:positive control),rice straw fed group(RS:negative control)and BF fed group(BF:treatment).Samples of rumen content,blood,rumen epithelium,muscle,feed offered and refusals were collected for the subsequent analysis.Results Feeding BF changed the microbial community and rumen fermentation,particularly increasing(P<0.05)relative abundance of Prevotella and propionate production,and decreasing(P<0.05)enteric methane yield.The histomorphology(height,width,area and thickness)of rumen papillae and gene expression for carbohydrate trans-port(MCT1),tight junction(claudin-1,claudin-4),and cell proliferation(CDK4,Cyclin A2,Cyclin E1)were improved(P<0.05)in sheep fed BF.Additionally,serum metabolome was also dynamically changed,which led to up-regulating(P<0.05)the primary bile acid biosynthesis and biosynthesis of unsaturated fatty acid in sheep fed BF.As a result,the higher(P<0.05)feed intake,digestibility,growth rate,feed efficiency,meat quality and mono-unsaturated fatty acid concentration in muscle,and the lower(P<0.05)feed cost per kg of live weight were achieved by feeding BF.Conclusions Feeding BF improved the growth performances and meat quality of sheep and reduced their feed cost.Therefore,bio-fermentation of rice straw could be an innovative way for improving ruminant production with mini-mizing production costs.

The impact of Internet access on household dietary quality:Evidence from rural China

Over the past few decades,the Internet has rapidly diffused across China.The spread of the Internet has had a profound economic and social impact on Chinese rural areas.Existing research shows that Internet access significantly impacts agricultural production and improves smallholder farmers’income.Beyond these,the Internet can affect other dimensions of social welfare.However,research about the impact of Internet access on dietary quality in rural China remains scarce.This study utilizes multi-period panel data from Fixed Observation Point in rural China from 2009 to 2015 to estimate the impact of Internet access on dietary quality and food consumption of rural households and conducts a causal analysis.Regression models with time and household fixed effects allow robust estimation while reducing potential issues of unobserved heterogeneity.The estimates show that Internet access has significantly increased rural household dietary quality(measured by the Chinese Diet Balance Index).Further research finds that Internet access has increased the consumption of animal products,such as aquatic and dairy products.We also examine the underlying mechanisms.Internet access improves dietary quality and food consumption mainly through increasing household income and food expenditure.These results encourage the promotion of Internet access as a valuable tool for nutritional improvements,especially in rural areas.

Mitochondrial dysfunction and quality control lie at the heart of subarachnoid hemorrhage

The dramatic increase in intracranial pressure after subarachnoid hemorrhage leads to a decrease in cerebral perfusion pressure and a reduction in cerebral blood flow.Mitochondria are directly affected by direct factors such as ischemia,hypoxia,excitotoxicity,and toxicity of free hemoglobin and its degradation products,which trigger mitochondrial dysfunction.Dysfunctional mitochondria release large amounts of reactive oxygen species,inflammatory mediators,and apoptotic proteins that activate apoptotic pathways,further damaging cells.In response to this array of damage,cells have adopted multiple mitochondrial quality control mechanisms through evolution,including mitochondrial protein quality control,mitochondrial dynamics,mitophagy,mitochondrial biogenesis,and intercellular mitochondrial transfer,to maintain mitochondrial homeostasis under pathological conditions.Specific interventions targeting mitochondrial quality control mechanisms have emerged as promising therapeutic strategies for subarachnoid hemorrhage.This review provides an overview of recent research advances in mitochondrial pathophysiological processes after subarachnoid hemorrhage,particularly mitochondrial quality control mechanisms.It also presents potential therapeutic strategies to target mitochondrial quality control in subarachnoid hemorrhage.

A Review of the Eco-Environmental Impacts of the South-to-North Water Diversion:Implications for Interbasin Water Transfers

Interbasin water-transfer schemes provide an engineering solution for reconciling the conflict between water demand and availability.In the context of climate change,which brings great uncertainties to water resource distribution,interbasin water transfer plays an increasingly important role in the global water–food–energy nexus.However,the transfer of water resources simultaneously changes the hydrological regime and the characteristics of local water bodies,affecting biotic communities accordingly.Compared with high economic and technical inputs water-transfer projects require,the environmental and ecological implications of water-transfer schemes have been inadequately addressed.This work selects the largest water-transfer project in China,the South-to-North Water Diversion(SNWD)Project,to critically review its eco-environmental impacts on donor and recipient basins,as well as on regions along the diversion route.The two operated routes of the SNWD Project represent two typical water diversion approaches:The Middle Route uses an excavated canal,while the East Route connects existent river channels.An overview of the eco-environmental implications of these two routes is valuable for the design and optimization of future water-transfer megaprojects.

Effect of low-degree astigmatism on the objective visual quality

AIM:To evaluate the effect of low-degree astigmatism on objective visual quality through the Optical Quality Analysis System(OQAS).METHODS:This study enrolled 46 participants(aged 23 to 30y,90 eyes)with normal or corrected-to-normal vision.The cylindrical lenses(0,0.5,0.75,1.0,and 1.25 D)were placed at the axial direction(180°,45°,90°,and 135°)in front of the eyes with the best correction to form 16 types of regular low-degree astigmatism.OQAS was used to detect the objective visual quality,recorded as the objective scattering index(OSI),OQAS values at contrasts of 100%,20%,and 9%predictive visual acuity(OV100%,OV20%,and OV9%),modulation transfer function cut-off(MTFcut-off)and Strehl ratio(SR).The mixed effect linear model was used to compare objective visual quality differences between groups and examine associations between astigmatic magnitude and objective visual quality parameters.RESULTS:Apparent negative relationships between the magnitude of low astigmatism and objective visual quality were observed.The increase of OSI per degree of astigmatism at 180°,45°,90°,and 135°axis were 0.38(95%CI:0.35,0.42),0.50(95%CI:0.46,0.53),0.49(95%CI:0.45,0.54)and 0.37(95%CI:0.34,0.41),respectively.The decrease of MTFcut-off per degree of astigmatism at 180°,45°,90°,and 135°axis were-10.30(95%CI:-11.43,-9.16),-12.73(95%CI:-13.62,-11.86),-12.75(95%CI:-13.79,-11.70),and-9.97(95%CI:-10.92,-9.03),respectively.At the same astigmatism degree,OSI at 45°and 90°axis were higher than that at 0°and 135°axis,while MTFcut-off were lower.CONCLUSION:Low astigmatism of only 0.50 D can significantly reduce the objective visual quality.

Influence of broken kernels content on soybean quality during storage

Although it is recognized that the post-harvest system is most responsible for the loss of soybean quality,the real impact of this loss is still unknown.Brazilian regulation allows 15%and 30%of broken soybean for group I and group II(quality groups),respectively.However,the industry is not informed about the loss in the quality parameters of soybeans and its impacts during long-term storage.Therefore,the objective was to evaluate the effect of the breakage kernel percentage of soybean stored for 12 months.Content of 15% of breakage kernels did not affect soybean quality.However,content of 30% of breakage kernels affected significantly soybean quality,which was evidenced by the increase of up to 75% in moldy soybeans,72% in acidity,50% in leached solids,27% in electrical conductivity,and the decrease of up to 12% in soluble protein,6.4% in germination and 3.5% in thousand kernel weight after 8 months of storage.Although the legislation establishes a percentage limit,it is recommended to store soybeans with up to 15% breakage kernels.On the contrary,values higher than that can cause a significant reduction in soybean quality,resulting in commercial losses.

Wild Nutrition’s Food-Grown® Magnesium Supplementation Increases Sleep Quality and Sleep Duration and Reduces Stress in a Healthy Adult Population: A Double-Blind, Randomised, Placebo-Controlled Study

Background: Magnesium, an essential mineral crucial for various bodily functions, has been shown to positively influence sleep patterns. This study aimed to evaluate the efficacy of Food-Grown® magnesium in enhancing sleep quality and duration, as well as overall well-being. Methods: Eighty participants were randomly assigned to receive either 80 mg of Food-Grown® magnesium or a placebo (microcrystalline cellulose) daily for 8 weeks. Participants completed questionnaires assessing sleep quality, daytime drowsiness, quality of life, anxiety, and stress levels. Additionally, participants maintained daily sleep diaries and wore wrist-worn actigraphy devices. The primary outcome measured was the change in sleep quality and duration. Results: Seventy-one participants fulfilled all study requirements (35 in the active group and 36 in the placebo group). Magnesium supplementation significantly improved reported sleep quality, with the active group showing a 32% increase compared to 16% in the placebo group (p = 0.034). Moreover, magnesium supplementation led to a decrease in reported stress scores at week 8 compared to the placebo group (3.7 ± 2.6 vs. 5.5 ± 3.1, respectively). Both the magnesium and placebo groups exhibited significant increases in reported sleep duration and reductions in time to fall asleep, sleep disturbance, sleep latency, sleep medication usage, and total Pittsburgh Sleep Quality Index score at week 8 compared to baseline. Conclusion: Magnesium supplementation notably enhanced sleep quality and reduced stress levels compared to the placebo group. These findings highlight the potential of magnesium as a beneficial supplement for improving sleep quality and overall well-being.

Genome wide association analysis identifies candidate genes for fruit quality and yield in Actinidia eriantha

Quality and yield are the primary concerns in kiwifruit breeding,but research on the genetic mechanisms of fruit size,shape,and ascorbic acid(ASA)content is currently very limited,which restricts the development of kiwifruit molecular breeding.In this study,we obtained a total of 8.88 million highly reliable single nucleotide polymorphism(SNP)markers from 140 individuals from the natural hybrid offspring of Actinidia eriantha cv.‘White’using whole genome resequencing technology.A genome-wide association study was conducted on eight key agronomic traits,including single fruit weight,fruit shape,ASA content,and the number of inflorescences per branch.A total of 59 genetic loci containing potential functional genes were located,and candidate genes related to single fruit weight,fruit length,ASA content,number of inflorescences per branch and other traits were identified within the candidate interval,such as AeWUSCHEL,AeCDK1(cell cycle dependent kinase),AeAO1(ascorbic oxidase)and AeCO1(CONSTANS-like 4).After constructing an RNAi vector for AeAO1 and injecting it into the fruit of cv.‘Midao 31’to interfere with the expression of the AeAO1 gene,the results showed that the activity of ascorbic oxidase in the fruit of‘Midao 31’significantly decreased,while the content of ASA significantly increased.This study provides valuable insights into the genetic basis of variation in A.eriantha fruit traits,which may benefit molecular marker-assisted breeding efforts.