ELECTRON MICROSCOPE ANALYSIS OF MITOCHONDRIA ON RENAL TUBULE CELL SUFFERED WARM ISCHEMIC AND REPER FUSIVE DAMAGE IN RABBITS

ＥＬＥＣＴＲＯＮＭＩＣＲＯＳＣＯＰＥＡＮＡＬＹＳＩＳＯＦＭＩＴＯＣＨＯＮＤＲＩＡＯＮＲＥＮＡＬＴＵＢＵＬＥＣＥＬＬＳＵＦＦＥＲＥＤＷＡＲＭＩＳＣＨＥＭＩＣＡＮＤＲＥＰＥＲＦＵＳＩＶＥＤＡＭＡＧＥＩＮＲＡＢＢＩＴＳＥＬＥＣＴＲＯＮＭＩＣＲＯＳＣＯＰＥＡ...

基于SEM的土体微观结构三维分析与分维计算方法

土体微观结构研究很早就建立了三维空间的分形模型,但分形维数计算所需参数很难由常规土工试验得到,限制了分形理论在土体研究中的应用。基于陕西定边与甘肃兰州原状土样的扫描电镜(SEM)试验,提出了三维化处理与三角形网格原理联合方法,可以得到土体颗粒(孔隙)不同测量尺度对应的表面积与体积计算值,由此实现分形维数的计算,并通过分形理论在土水特征中的应用验证了方法的有效性与可靠性。另外,三维化处理还可获取视孔隙率、比面等土体微观结构参数,可以作为土体微观结构定量分析研究的新途径。

Chalkiness Characters and Scanning Electron Microscope Observation of Rice Grain Endosperm of Japonica Varieties in Southern Henan

Chalkiness characters affect not only the grain appearance,milling,eating and cooking qualities but also the grain nutritional quality in rice,thus it is one of the most important traits in rice. It is very important for us to investigate the relation of the chalkiness formation and the development of endosperm structure and starch granule of different rice varieties. Here,we have investigated the chalkiness characters such as chalkiness rate,chalkiness degree and chalkiness area in 15 japonica rice varieties from southern Henan. Furthermore,the endosperm structure and starch granules of rice grain were also observed with scanning electron microscope. The results showed that the 15 japonica varieties have a significantly linear relationship between the chalkiness rate and chalkiness degree. Among the varieties,the biggest difference is the chalkiness rate,the second is the chalkiness area,and the last is the chalkiness degree. Moreover,there is a certain correlation between the distribution of starch granules,the arrangement of endosperm cells and the occurrence of grain chalkiness in the different rice varieties. For the same variety,the starch granules of chalky and non-chalky grains have obvious difference,while the starch granules from the transparent part of chalky rice and non-chalky rice do not have significant difference. The results would provide useful references for the improvement of grain quality in rice.

OBSERVATION OF THE EFFECT OF S－（－）USNIC ACID SODIUM ON TRICHOMONAS VAGINALIS BY TRANSMISSION ELECTRON MICROSCOPE

This paper reports the ultrastructural changes of trichomonas vaginaiis (T. vag. ) under the action of s-(-)usnic acid sodium in vitro. These changes can be shown by the following results:At first, the degranulation of the rough endoplasmic reticulum (RER) took place, the polyribosomes disaggregated. And then, the Golgi complex and the endoplasmic reticulum dilated. The cytoplasmic matrix presented and inhomogeneous apperance. Finally, the biomembrane loosed and fractured.The cell nuclei presented the karyorrhexis.

Acoustical properties of a 3D printed honeycomb structure filled with nanofillers:Experimental analysis and optimization for emerging applications

The novelty of this research lies in the successful fabrication of a 3D-printed honeycomb structure filled with nanofillers for acoustic properties,utilizing an impedance tube setup in accordance with ASTM standard E 1050-12.The Creality Ender-3,a 3D printer,was used for printing the honeycomb structures,and polylactic acid(PLA)material was employed for their construction.The organic,inorganic,and polymeric compounds within the composites were identified using fourier transformation infrared(FTIR)spectroscopy.The structure and homogeneity of the samples were examined using a field emission scanning electron microscope(FESEM).To determine the sound absorption coefficient of the 3D printed honeycomb structure,numerous samples were systematically developed using central composite design(CCD)and analysed using response surface methodology(RSM).The RSM mathematical model was established to predict the optimum values of each factor and noise reduction coefficient(NRC).The optimum values for an NRC of 0.377 were found to be 1.116 wt% carbon black,1.025 wt% aluminium powder,and 3.151 mm distance between parallel edges.Overall,the results demonstrate that a 3Dprinted honeycomb structure filled with nanofillers is an excellent material that can be utilized in various fields,including defence and aviation,where lightweight and acoustic properties are of great importance.

ELECTRON MICROSCOPE EVIDENCE OF VIRUS INFECTION IN CULTURED MARINE FISH

Electron microscope investigation on the red sea bream ( Pagrosomus major ), bastard halibut ( Paralichthys olivaceus ) and stone flounder ( Kareius bicoloratus ) in North China revealed virus infection in the bodies of the dead and diseased fish. These viruses included the lymphocystis disease virus (LDV), parvovirus, globular virus, and a kind of baculavirus which was not discovered and reported before and is now tentatively named baculavirus of stone flounder ( Kareius bicoloratus ).

Detection of Bisphenol A (BPA) in Plastic Bottles Using Vertical Cultivation at Various Temperatures

Polycarbonate plastics containing bisphenol A (BPA) used to manufacture drinking water bottles. Kurdistan region in northern Iraq is a developed area with increased pollution from plastic bottles. Trace amounts of BPA have been detected in bottled water samples. The absorption of BPA was measured with HPLC using a vertical cultivation system with Bulbs of the Allium Cepa plant planted in these plastic bottles with monitored growth. Vertical cultivation was found to have a low level of BPA in the plant cells, making it a safe cultivation method under specific climate conditions. The mean concentration of BPA in vertical cultivation is 0.19 ug/ml (3.8 ng for a 20 uL injection), and the Limit of Quantification (LOQ) is 0.63 ug/ml (12.7 ng for 20 uL injection). While Scanning Electron Microscope (SEM) shows that the concentrations are relatively low in water samples stored at room temperature compared to those exposed to direct sunlight (40°C) and water bottle samples stored at (-4°C), The correlation coefficients were found to be good (0.9992). SEM is used for plastic bottle samples stored at different temperatures. The images identify compound decay and explore the morphology of BPA in manufactured plastic materials.

Scanning Electron Microscopic Observation on Morphologic Characteristics of Sperms in Uremic Patients

To observe the morphologic characteristics of spermatozoon ultramicroscopic structure in uremic subjects.Method Semen sample from lO patients with uremia and 5 healthy men were observed under light microscope and scanning electronic microscope.Results Abnormalities were found in sperms of uremic patients either in the sperm head （acrosome, acrosomic deficit, nuclear abnormality, pointed head, headless and double head of spermatozoon）, neck （rupture, separation and enlargement）, or tail （mitochondrial swelling, mitochondrial deficit, tailless, double tail, short tail and curled tail）; whereas none of the above-mentioned abnormalities was observed in healthy men. Conclusion Sperms of uremic patients had many morphologic and structural abnormalities in the head, neck and tail.

Characterization of Li-rich layered oxides by using transmission electron microscope

Lithium-rich layered oxides(LrLOs) deliver extremely high specific capacities and are considered to be promising candidates for electric vehicle and smart grid applications. However, the application of LrLOs needs further understanding of the structural complexity and dynamic evolution of monoclinic and rhombohedral phases, in order to overcome the issues including voltage decay, poor rate capability, initial irreversible capacity loss and etc. The development of aberration correction for the transmission electron microscope and concurrent progress in electron spectroscopy, have fueled rapid progress in the understanding of the mechanism of such issues. New techniques based on the transmission electron microscope are first surveyed, and the applications of these techniques for the study of the structure, migration of transition metal, and the activation of oxygen of LrLOs are then explored in detail, with a particular focus on the mechanism of voltage decay.

Gill surface architecture of mullet, Mugil soiuy (Basilewsky) : A scanning electron microscope study

Specimens of Mugil soiuy were collected from the coastal waters of Bohai Sea in Tianjin. The gillfilaments were cut with fine scissors and soaked for 24h in a fixative consisting of 2.5% glutaralde-hyde in 0.1 mol lphosporic acid buffer. After three washes in a buffer solution the tissue was fixedin a mixture of 1% osmium tetraoxide at 4℃ for 1h. The tissue was dehydrated in graded ethandethanols and dried. The tissue was examined and photographed with an SEM at an accelerating voltage

ELECTRON MICROSCOPIC OBSERVATION OF DIFFERENTIATION OF LEUKEMIC CELL CLONE AFTER CFU-MIX CULTURE

By scanning and transmission electron microscopy, leukemlc celb were obwrved after CFU-Mix culture. Even though granulocytlc growth factor, erythropoietin and lymphocytlc growth factor were added at vitor, acute leukemlc celb still showed defects In differentiation and maturation. These were characterized by abnormal colony which consisted of smooth cells, bizarre shape, nuclear-cytoplasmic asynchrony In development, and appearance of nuclear bleb. However, chronic myelogenous leukemlc celb were more nature than the acute ones, manifesting in normal colony with finger- like projections and ruffled membrane. Macrophages and eosinophils could be observed. It b suggested that there b a difference In differentiation between acute and chronic leukemia cells.

COEXISTENCE OF OSTEOARTHRITIS AND OSTEOPOROSIS IN FEMORAL HEAD: A SCANNING ELECTRON MICROSCOPIC STUDY

Objective To ascertain the coexistence of osteoarthritis and osteoporosis in the femoral head. Methods During total hip replacement for osteoarthritis of hip joint in 12 female patients with an average age of 56 years, the femoral heads were procured. After processing the femoral had, specimens were studied under scanning electron microscope. Results Different degrees of osteoarthritic lesions (characterized by degeneration of articular cartilage and hyperplasia of subchondral bone tissues) could coexust with osteoporotic lesions (characterized by icicle-like trabeculae ) in the femoral had. The hyperplas- tic bone tissues of osteoarthritis lay in the vicinity of icicle-like trabeculae. However, they did not care into contact, nor did they exert influence on each other. Coexistence of these two different lesions covered a brood territory extending from the deep surface of subchondral bone plate to the head-neck junction of femoral head. Conclusion The results suggest that osteoarthritis and osteoporosis do coexist in the femoral head.

Observation of healing between artificial bladder and tissue by scanning electronic microscope

Object To detect the healing between artificial bladder and tissue. Method:Healing between 4 artificial bladder and surrounding tissue was observed by scanning electronic microscope. Result 4 prosthesis all could heal with tissue,but healing extent was different during different time. Conclusion:This artificial bladder could perform a good connection with tissue and the structure of nylon, surface area of prosthesis, time and infection maybe influenced the healing.

致密砂岩碳酸盐矿物SEM-CL和EPMA矿物学表征及其成岩意义——以川西坳陷须家河组为例

扫描电镜—阴极发光(SEM-CL)可通过同时分析矿物的微观结构和阴极发光特性来反映成岩机制及流体来源,但碳酸盐矿物在SEM-CL下的发光特性及其影响因素尚不明确。应用光学显微镜阴极发光(OM-CL)、电子探针(EPMA)和SEM-CL等技术手段,以川西坳陷须家河组致密砂岩中碳酸盐矿物为研究对象,分析碳酸盐矿物在OM-CL和SEM-CL下发光特性的差异,评估影响碳酸盐矿物SEM-CL发光特性的因素,探讨砂岩中方解石胶结物的成岩意义。结果表明,相比OM-CL,SEM-CL在区分碳酸盐岩屑和碳酸盐胶结物方面具有更高的灵敏度。当Mn/Fe值低于0.1或Mn/Fe值介于0.1~0.5且Fe含量低于0.1%时,碳酸盐矿物具有中低强度的SEM-CL发光强度;当Mn/Fe值高于0.5且Fe含量高于0.1%时,碳酸盐矿物在SEM-CL照射下几乎不发光。Mn/Fe值和Fe含量是影响SEM-CL照射下碳酸盐矿物发光强度的主要因素。阴极发光分析和EPMA元素组成面扫描分析表明,方解石胶结物的元素分布是均质的,形成于同一成岩世代,晚于长石等矿物的溶解。岩屑砂岩和岩屑石英砂岩的方解石胶结物具有相同的来源,表现为高Mn/Fe特征;钙屑砂岩的方解石胶结物具低Mn高Fe特征,成分组成与白云石岩屑相似,推测流体可能受到白云石岩屑溶解的影响。

Bacteriological and electron microscopic examination of primary intrahepatic stones

BACKGROUND: Primary intrahepatic cholelithiasis is usually combined with biliary tract infection. This research was undertaken to investigate the relationship between intrahepatic stones and biliary tract infection. METHODS: Thirty-five bile samples and 30 stones specimens were cultured for bacteria and 12 stones specimens were examined with a scan electron microscope (SEM) or a transmission electron microscope (TEM). RESULT: 94.2% bile samples and 96.7% stones specimens were positive in bacteria culture. Bacteria were found in stones under SEM and TEM. CONCLUSION: Bacteria in stones are associated with the infection of the biliary tract.

Electron Microscopic In－situ Observation for Preparation of Ultrafine La_2O_3 Nanophase Powder

In-situ observation in high voltage electron microscope(HVEM) was used to investigate transformation from amorphous La(OH)3 to nanophase La2O3. Electron diffraction pattern indicates that the transition temperature from La(OH)3 to nanophase La2O3 is 400 ℃. Particle sizes for nanophase La2O3 are from 20 to 50 nm.

Nanoelectronic devices resonant tunnelling diodes grown on InP substrates by molecular beam epitaxy with peak to valley current ratio of 17 at room temperature

This paper reports that InAs/In0.53Ga0.47As/AlAs resonant tunnelling diodes have been grown on InP substrates by molecular beam epitaxy. Peak to valley current ratio of these devices is 17 at 300K. A peak current density of 3kA/cm^2 has been obtained for diodes with AlAs barriers of ten monolayers, and an Ino.53Ga0.47As well of eight monolayers with four monolayers of InAs insert layer. The effects of growth interruption for smoothing potential barrier interfaces have been investigated by high resolution transmission electron microscope.

SCANNING ELECTRON MICROSCOPIC STUDY OF FETAL CHICKEN CALVARIAL OSTEOBLAST-LIKE CELLS CULTURED IN VITRO

Three types of osteoblast-like cells with different cnfigurations could be ob-tained through culturing fetal chicken calvaria in vitro. They were spindle-shaped cells,globular cells, and polygonal or squamous cells. With passage of culture time, there werechanges in configuration so that the spindle-shaped cells and the globular cells turnedgradually into squamous cells, in quantity which increased greatly to produce confluenceand multi-layer formation of cells, and in function as evidenced by emergence ofintracytoplasmic granules, reflecting collagen synthesis.

Ultrastructural view of colon anastomosis under propolis effect by transmission electron microscopy

AIM: To evaluate the effect of propolis administration on the healing of colon anastomosis with light and transmission electron microscopes. METHODS: Forty-eight Wistar-Albino female rats were divided into two groups and had colon resection and anastomosis. In group Ⅰ, rats were fed with standard rat chow pre- and postoperatively. The rats in group Ⅱ were fed with standard rat chow and began receiving oral supplementation of propolis 100 mg/kg per day beginning 7 d before the operation and continued until they were sacrificed. Rats were sacrificed 1, 3, 7 and 14 d after operation, and anastomotic bursting pressures measured. After the resection of anastomotic segments, histopathological examination was performed with light and transmission electron microscopes by two blinded histologists and photographed. RESULTS: The colonic bursting pressures of the propolis group were statistically significantly better than the control group. Ultrastructural histopathological analysis of the colon anastomosis revealed that propolis accelerated the phases of the healing process and stimulated mature granulation tissue formation and collagen synthesis of fibroblasts. CONCLUSION: Bursting pressure measurements and ultra structural histopathological evaluation showedthat administration of propolis accelerated the healing of colon anastomosis following surgical excision.

A LIGHT AND TRANSMISSION ELECTRON MICROSCOPIC STUDY OF HEXAMITE CAPSULARIS SP.NOV.(DIPLOMONADINA:HEXAMITIDAE) IN FISH (XENOCYPRIS DIVIDI)

A diplomonad flagellate Hexamita capsularis sp.nov.from the posterior intestine of the fish(Xenocypris dividi)from Liangzi Lake,Hubei Province,is described.The flagellate was oval,withsmooth surface and homogenous cytoplasm,6.21±0.28μm in length,3.43±0.48μm in width and with anelliptical capsule.Two recurrent flagellae arranged between two nuclei ran longitudinally to the posteriorend.Fibrillar bands connected the accessory organelles of two cytostomal tubes,the body depressed atthe posterior end of the body.