A new ellagitannin from Cleidion bracteosum

A new ellagitannin, named bractin （1）, had been isolated from the dried aerial part of Cleidion bracteosum. Its structure was determined based on various of spectral analysis.

Antifungal ellagitannin isolated from Euphorbia antisyphilitica Zucc

Objective:To study antifungal activity of a new ellagitannin isolated from the plant residues of Euphorbia antisyphilitica(E.antisyphilitica)Zucc in the wax extraction process.Methods:An extract was prepared from dehydrated and pulverized residues and fractionated by liquid chromatography on Amberilte XAD-16,until obtained an ellagitannin-rich ethanolic fraction which was treated by rotaevaporation to recover the ellagitannin as fine powder.An aqueous solution was prepared and treated through ionic exchange liquid chromatography(Q XL)and gel permeation chromatography(G 25).The ellagitannin-rich fraction was thermogravimetrically evaluated(TGA and DTA)to test the thermo-stability of ellagic acid(monomeric unit).Then ellagitannin powder was analyzed by infrared spectrospcopy to determinate the functional groups and.also mass spectroscopy was used to determine the molecular ion.Results:The principal functional groups of ellagitannin were determined,the molecular weight was 860.7 g/mol;and an effective antifungal activity against phytopathogenic fungi was demonstrated.Conclusions:It can be concluded that the new ellagitannin(860.7 g/mol)isolated from E.antisyphilitica Zucc is an effective antifungal agent against Alternaria alternata,Fusarium oxyzporum,Colletotrichum gloeosporoides and Rhizoctnia solani.

Efficacy of ellagic acid and ellagitannins on diabetes mellitus:A meta-analysis of preclinical and clinical trials

A meta-analysis of 13 preclinical trials and 11 clinical trials to assess efficacy of ellagic acid and ellagitannins on diabetes mellitus was conducted.Seven biomarkers of interest closely related with diabetes mellitus were monitored in the studies.The mean difference and 95%confidence interval were pooled using random-effect model.Heterogeneity test was conducted using Cochrane Q-test and Higgins I2 test,and Funnel plots were used to detect publication bias.High heterogeneity was found for both preclinical and clinal trials,with I2 levels above 75%.Preclinical studies found significant reductions in fasting glucose level(p<0.001),glycated hemoglobin(p=0.008),total cholesterol(p<0.001),and low-density lipoprotein(p=0.018),but insignificant changes in triglycerides(p=0.057),insulin(p=0,223),and high-density lipoprotein(p=0.144).Clinical trials found a significant increase in high-density lipoprotein(p=0.034))and a significant decrease in glycated hemoglobin(p=0.02),but insignificant changes were reported for fasting glucose level(p=0.090),insulin(p=0.164),homeostasis model assessment of insulin resistance(p=0.328),total cholesterol(p=0.977),triglycerides(p=0.834),and low-density lipoprotein(p=0.888).The results support ellagic acid and ellagitannins showing some efficacy in diabetes mellitus intervention.

藏药余甘子化学成分研究

目的 :研究藏药余甘子的化学成分。方法 :各种色谱法分离纯化 ,理化和现代波谱法鉴定结构。结果 :分离鉴定出 11个化合物 ,分别为没食子酸 (Ⅰ ) ,鞣花酸 (Ⅱ ) ,1 O 没食子酰基 β D 葡萄糖 (Ⅲ ) ,3,6 二 O 没食子酰基 D 葡萄糖 (Ⅳ ) ,诃子酸 (Ⅴ ) ,槲皮素 (Ⅵ ) ,诃黎勒酸 (Ⅶ ) ,鞣云实精 (Ⅷ ) ,3 乙基没食子酸 (3 ethoxy 4 ,5 dihydroxy benzoicacid ,Ⅸ ) ,isostrictiniin(Ⅹ ) ,1,6 二 O 没食子酰基 β D 葡萄糖 (Ⅺ )。 结论 :3 乙基没食子酸 (3 ethoxy 4 ,5 dihydroxy benzoicacid)为一新化合物 ,isostrictiniin为首次从余甘子中得到的化合物。

核桃仁中多酚类物质的液相／电喷雾质谱分析

利用HPLC-ESI-MSn方法对核桃(Juglans regiaL.)仁中多酚类物质组成进行了分析,发现的多酚类物质有鞣花酰基葡萄糖4种异构体、橡椀?咸烟?种异构体、二鞣花酰基葡萄糖2种异构体、二没食子酰基葡萄糖2种异构体、鞣花酰基和橡椀?咸烟?种异构体。结果表明,采用HPLC-ESI-MSn方法可以对核桃仁中多酚类物质进行有效的分析。

叶下珠中新多酚化合物的分离与鉴定

目的：研究植物药叶下珠（ＰｈｙｌａｎｔｈｕｓｕｒｉｎａｒｉａＬ．）的化学成分。方法：用各种色谱技术进行分离纯化，经ＭＳ，１Ｈ，１３ＣＮＭＲ和ＨＭＢＣ光谱分析鉴定其结构。结果：分得４个多酚类化合物，其结构分别鉴定为１Ｏ没食子酰基３，６六羟基联苯二甲酰基２，４去羟甲基诃子酰基吡喃葡糖（Ｉ），焦酚（ｐｙｒｏｇａｌｏｌ，Ｉ），咖啡酸（ｃａｆｅｉｃａｃｉｄ，ＩＩ）和（ｂｒｅｖｉｆｏｌｉｎｃａｒｂｏｘｙｌｉｃａｃｉｄ，ＩＶ）。结论：化合物Ｉ为一新的鞣花单宁（命名为ｐｈｙｌａｎｔｈｕｓｉｎＵ），Ｉ，ＩＩ，ＩＶ为首次从该植物中分得。

叶下珠新鞣花鞣质的分离与鉴定(英文)

目的 研究叶下珠 (PhyllanthusurinariaL .)的化学成分。方法 用多种色谱法进行分离纯化 ,现代波谱法鉴定结构。结果 分离得到一个新的鞣花鞣质叶下珠素G ,结构为 1 O galloyl 2 phyllanthoyl 3 ,6 (R) HHDP β D glucose。结论 叶下珠素G(phyllanthusiinG)

广西甜茶多酚提取工艺研究

通过对水抽提、醇抽提、水提碱转法三种甜茶多酚抽提工艺进行对比,得出水提碱转法为较好的一种抽提方法。通过单因素和正交实验(L16(44))得出:影响甜茶多酚提取效率的主要因素为抽提时间,其次为pH,受提取温度和料液比的影响小;最佳提取工艺为提取温度85℃,时间30min,pH8.5,料液比1∶10,在此条件下的甜茶多酚得率为3.91%。

没食子鞣质,1,3,4-三-O-没食子酰基-6-O-咖啡酰基-β-D-吡喃葡萄糖,抑制人肝癌HepG_2细胞的增殖和调控miRNA的表达(英文)

目的 miRNA在细胞的增殖,分化和凋亡中起重要作用。1,3,4-三-O-没食子酰基-6-O-咖啡酰基-β-D-吡喃葡萄糖(BJA32515)是从日本蛇菰中分离的天然多酚化合物,该化合物对肿瘤细胞的增殖和miRNA的影响未见报道。本文旨在研究BJA32515对人肝癌细胞HepG2增殖的抑制作用以及对miRNA表达的影响。方法采用CCK-8的方法检测HepG2细胞的增殖;用流式细胞法检测HepG2细胞的凋亡;采用miRNA芯片分析方法测定HepG2细胞的miRNA表达以及用RT-PCR的方法验证miRNA的表达。结果 BJA32515以时间和剂量依赖的方式抑制HepG2细胞的增殖,并促进细胞的凋亡。miRNA芯片结果显示,BJA32515能诱导HepG2细胞33个miRNA的表达上调以及59个miRNA的下调。RT-PCR结果也证实BJA32515可诱导let-7a和miR-29a的上调以及miR-373和miR-197的下调,与芯片分析的结果一致。结论BJA32515抑制HepG2细胞增殖的作用机制与miRNA的表达调控有关。

榛子叶化学成分研究(Π)

目的 对榛子叶中的鞣质成分进行了研究 ,开发利用黑龙江省榛子叶资源。方法 利用 1 H- NMR,1 3C- NMR,FAB- MS,CD谱及 1 H- 1 HCOSY二维谱与化学方法鉴定。结果 从中得到 4个化学成分 :榛叶素 B(heterophylliin B, ) ,tellimagrandin ( ) ,casuarictin ( ) ,casuarinin ( )。结论 榛叶素

鞣花鞣质类成分研究进展

鞣花鞣质(ellagitannin)是一类复杂的植物多酚类化合物,是许多常用天然药物的药效物质,被广泛应用于医药、化妆品、食品、保健品等多个领域。本文就鞣花鞣质的结构来源、生物活性和代谢转归进行综述,为富含鞣花鞣质类有效成分的天然药物的深入研究和资源开发提供依据。

国产橡木与欧美橡木主要成分的差异分析

对蒙古栎和辽东栎两种国产橡木中影响葡萄酒陈酿的主要成分进行分析,并与法国的无柄橡和美国白橡进行对比。结果表明,蒙古栎中鞣花单宁、总酚、反式橡木内酯以及鞣花酸和没食子酸的含量与美国橡木较为接近,且顺反橡木内酯之间的比值也与美国橡木近似,但顺式橡木内酯的含量显著低于美国橡木,而与法国橡木接近;而辽东栎无论是鞣花单宁和总酚的含量,还是顺反橡木内酯以及低分子酚类化合物的含量,在所测试的4种橡木中,均表现为最低,且与法国和美国橡木相比具有显著差异。因此,从橡木用于葡萄酒陈酿的潜力来看,蒙古栎是更适合开发国产橡木桶的树种。

粗叶悬钩子Rubus aleaefolius Poir.中三个新的细胞周期抑制剂 :一个新的和两个已知的水解鞣质(英文)

通过跟踪分离活性成分 ,从粗叶悬钩子RubusaleaefoliusPoir .中分到一个新的鞣花酸鞣质化合物 1和两个已知的没食子酸鞣质 1,2 ,3,6 tetra O galloyl β D glucopyranose(2 )和 1,2 ,3,4 ,6 penta O galloyl β D glucopyranose(3) ,并通过谱学手段确定了新化合物 1的化学结构。化合物 1～ 3将tsFT2 10癌细胞的细胞周期抑制在G0 /G1期 ,MIC值分别为2 2 1μmol·L-1(1) ,7 9μmol·L-1(2 )和 6 6 μmol·L-1(3) ,系三个新的G0 /G1期抑制剂。化合物 1～ 3作为鞣质类化合物的细胞周期抑制剂亦属首例 。

Potential of the ellagic acid-derived gut microbiota metabolite–Urolithin A in gastrointestinal protection

Urolithin A(UA)is a metabolic compound generated during the biotransformation of ellagitannins by the intestinal bacteria.The physiologically relevant micromolar concentrations of UA,achieved in the plasma and gastrointestinal tract(GI)after consumption of its dietary precursors,have been revealed to offer GI protection.The health benefit has been demonstrated to be principally related to anticancer and anti-inflammatory effects.UA has been shown to possess the capability to regulate multiple tumor and inflammatory signaling pathways and to modulate enzyme activity,including those involved in carcinogen biotransformation and antioxidant defense.The purpose of this review is to gather evidence from both in vitro and in vivo studies showing the potential of UA in GI protection alongside suggested mechanisms by which UA can protect against cancer and inflammatory diseases of the digestive tract.The data presented herein,covering both studies on the pure compound and in vivo generated UA form its natural precursor,support the potential of this metabolite in treatment interventions against GI ailments.

Polyphenolic content, in vitro antioxidant activity and chemical composition of extract from Nephelium lappaceum L.(Mexican rambutan) husk

Objective: To determinate the recovery of total polyphenolic compounds content,in vitro antioxidant activity and HPLC/ESI/MS characterization of extract from Nephelium lappaceum L.(Mexican rambutan).Methods: The rambutan husk extract was obtained by aqueous extraction and a polyphenolic fraction was recovered using Amberlite XAD-16. The total polyphenolic compounds content was determined by the Folin Ciocalteu and butanol-HCI methods.In vitro antioxidant activity was performed using ABTS and ferric reducing antioxidant power methods.Results: Mexican rambutan husk showed a total polyphenolic content of 582 mg/g and an evident antioxidant activity by ABTS and ferric reducing antioxidant power analysis.The HPLC/ESI/MS assay allowed the identification of 13 compounds, most of which belong to ellagitannins. Geraniin, corilagin and ellagic acid were present in the sample;the mineral composition was also evaluated.Conclusions: Rambutan husk cultivated in Mexico is a promising source for the recovery of added value bioactive compounds with antioxidant activity, which have potential applications as bioactive antioxidant agents for the treatment of diseases.

柠檬桉叶化学成分研究(四报)——单宁组分研究

从柠檬桉叶醇提取物中分离和鉴定了倍酸,鞣花酸和栗木鞣花素等化合物。研究表明柠檬桉叶单宁为鞣花单宁类化合物,并有较多的C-甙类鞣花单宁,这类单宁系首次从桉树属植物发现。

蒙古栎主要风味成分在风干过程中的变化

对蒙古栎橡木在室外自然风干过程中糅花单宁、总酚和挥发性香气成分的含量的变化进行了分析。结果表明:风干2年即可使总酚以及部分挥发性化合物达到稳定,但鞣花单宁和具有不良气味的化合物如壬烯醛、苯酚等变化较缓慢,需3年的时间才能达到稳定水平。而风干时间达4年时,酚醛以及糠醛等化合物的含量由于氧化、挥发等原因则下降。因此,蒙古栎适宜的风干时间确定为3年。

鞣花酸的制备及应用研究进展

目的:综述了鞣花酸和鞣花单宁的结构和来源、鞣花酸的提取和生物法制备工艺的研究进展,以期为鞣花酸的工业生产提供科学依据。方法:总结有关鞣花酸制备的研究性论文、综述及书籍等。结果:固态发酵、液态发酵和酶转化方法生产鞣花酸的工艺优缺点各异。结论:鞣花酸的应用前景广阔,生产方式多样,生物技术法生产鞣花酸是一种绿色高效的生产的方法。

红树莓中酚类化合物研究进展

介绍了红树莓中花青素、黄酮和鞣质(鞣花酸)3种酚类化合物,探讨了国内外关于3种酚类化合物的提取、分离纯化和性质、功能活性及产品开发的研究现状,展望了红树莓3种酚类化合物的研究前景。

红毛丹果皮中老鹳草素的分离纯化及其热稳定性

老鹳草素是一种天然来源的、具有显著生物活性的可水解鞣花单宁,前期研究发现红毛丹果皮富含老鹳草素。为了提高红毛丹果皮的利用率,本实验研究了中压液相色谱反相C_(18)柱系统和制备型高效液相色谱对红毛丹果皮多酚制取物中老鹳草素的快速分离纯化技术,采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱系统对老鹳草素的热稳定性及其热降解产物进行了定性分析。结果发现真空冷冻干燥的红毛丹果皮中老鹳草素含量高达12.67 g/100 g(以果皮干质量计);其多酚制取物经中压液相色谱反向C_(18)柱系统和制备型高效液相色谱系统分离和洗脱,得到纯度为95.63%的老鹳草素产品,产率为6.00%(以果皮干质量计)。纯化后的老鹳草素不耐高温,60℃处理10 h,老鹳草素可降解损失17.13%;100℃处理2 h,降解损失率为73.97%。在老鹳草素的热降解体系中鉴定出没食子酸、柯里拉京、六羟基联苯二甲酸、云实酸、Galloyl-bis-HHDP-glucose、鞣花酸、云实素等分子质量更低的酚类物质。研究结果可为老鹳草素的可控降解及生物利用提供探讨。