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Investigation of the Low Molecular Weight Thiol Composition in a Metastatic Prostate Cancer Cell Line (LNCaP) by LC-UV-MS and NMR after Labelling with the Ellman Reagent
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作者 Stephen Childs Nicolas Haroune +1 位作者 Lee Williams Michael Gronow 《American Journal of Analytical Chemistry》 2017年第1期1-18,共18页
The low molecular weight thiols present in the deproteinized extract of a prostate cancer cell line (LNCaP-FGC) were analysed after derivatization with the Ellman reagent (ESSE). The mixed disulphides formed (RSSE) we... The low molecular weight thiols present in the deproteinized extract of a prostate cancer cell line (LNCaP-FGC) were analysed after derivatization with the Ellman reagent (ESSE). The mixed disulphides formed (RSSE) were fractionated, characterized and quantified by liquid chromatography on a C-18 column using UV detection. This revealed the presence, in femtomoles per cell, of glutathione (8.30 ± 0.73), cysteine (2.71 ± 0.04) and cysteinylglycine (0.83 ± 0.10), accounting for the bulk of the thiol present. Further analysis of the cell extracts using a novel and sensitive mass spectrometry technique allowed the detection of low level of an additional derivative which was identified as cysteinylglycerate using NMRspectroscopy. 展开更多
关键词 Prostate Cancer Low Molecular Weight THIOLS THIOL Analysis Ultra-High Performance Liquid Chromatography Novel THIOL LC-MS NMR ellman’s reagent
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Ellman’s试剂测定脱脂豆粕中巯基含量 被引量:11
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作者 田其英 谌卉 华欲飞 《食品与生物技术学报》 CAS CSCD 北大核心 2008年第6期107-110,共4页
采用3种不同方法对干热处理后的脱脂豆粕进行处理,再加入Ellman’s试剂来测定脱脂豆粕中的巯基含量。通过理论和数据分析得到,方法二即脱脂豆粕、尿素-SDS溶液和Ellman’s试剂相混合反应可用于测定脱脂豆粕中的巯基含量。
关键词 脱脂豆粕 ellman’s试剂 巯基含量 方差分析
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Effect of reagent films on coupling relationship between glass surface and particles 被引量:1
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作者 李明 吴超 +1 位作者 刘一静 闫晖 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第11期2799-2805,共7页
The experiments were conducted to investigate the behavior of airborne particles adhering to the glass slides which were coated by several reagent films. The results showed that the adhesion level could be significant... The experiments were conducted to investigate the behavior of airborne particles adhering to the glass slides which were coated by several reagent films. The results showed that the adhesion level could be significantly changed by the reagent films. There were no evident rules between the average size of particles and sampling time interval, the placing angle and reagent concentration. The average particle size on the surface coated by composite reagent (2-3 μm) was smaller than that on the single reagent coated surface, while the largest particle size (4-5 μm) was observed on the surface coated with the Tween 60. The experiment also demonstrated that the best adhesive performance was obtained on the surface which was coated with 0.5% SDBS and 0.5% fluorocarbon composite reagents. The experiment results indicated that each reagent had a certain optimum adhesive range to the particle. The composite reagents with different proportion of single reagents exhibited some particular physical and chemical properties, which could effectively change the adhesive performance between the solid surface and the particles. 展开更多
关键词 micro-particles ADHESION glass surface reagent film size distribution
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改良Ellman法检测血液胆碱酯酶的初步探讨 被引量:1
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作者 焦路阳 刘彦慧 赵玉洲 《新乡医学院学报》 CAS 1999年第2期176-177,共2页
笔者采用碱性羟胺法[1]和改良Elman法[2]测定30例有机磷农药中毒患者血液胆碱酯酸,把两法进行比较,报告如下。1对象与方法11对象1997年在我院诊治的有机磷农药中毒患者,中毒途径为消化道和皮肤吸收。12方... 笔者采用碱性羟胺法[1]和改良Elman法[2]测定30例有机磷农药中毒患者血液胆碱酯酸,把两法进行比较,报告如下。1对象与方法11对象1997年在我院诊治的有机磷农药中毒患者,中毒途径为消化道和皮肤吸收。12方法在患者入院12~24h内同时采取... 展开更多
关键词 ellman 检测 有机磷农药中毒 血液 胆碱酯酶
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芬顿试剂(Fenton's reagent法降解对苯二酚和工业废水 被引量:4
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作者 常文贵 谢红璐 《渤海大学学报(自然科学版)》 CAS 2004年第1期15-18,共4页
可溶性亚铁盐和H2O2的组合称为芬顿试剂(Fenton’s reagent),是一种氧化能力很强的氧化剂,常用于废水的净化处理。本文以对苯二酚为代表物,探讨用Fenton试剂氧化的反应条件,并用最佳的实验条件处理工业废水,90分钟工业废水的COD去除率可... 可溶性亚铁盐和H2O2的组合称为芬顿试剂(Fenton’s reagent),是一种氧化能力很强的氧化剂,常用于废水的净化处理。本文以对苯二酚为代表物,探讨用Fenton试剂氧化的反应条件,并用最佳的实验条件处理工业废水,90分钟工业废水的COD去除率可达71.6%。 展开更多
关键词 芬顿试剂 羟基自由基 废水处理 COD去除率 苯二酚 工业废水
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Preparation of ZnO Nanoparticles and Photocatalytic H2 Production Activity from Different Sacrificial Reagent Solutions
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作者 彭天右 吕红金 +1 位作者 曾鹏 张晓虎 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2011年第4期464-470,I0004,共8页
ZnO nanoparticles were synthesized via a direct precipitation method followed by a hetero- geneous azeotropic distillation and calcination processes, and then characterized by X-ray power diffraction, scanning electro... ZnO nanoparticles were synthesized via a direct precipitation method followed by a hetero- geneous azeotropic distillation and calcination processes, and then characterized by X-ray power diffraction, scanning electron microscopy, transmission electron microscopy, and ni- trogen adsorption-desorption measurement. The effects of Pt-loading amount, calcination temperature, and sacrificial reagents on the present ZnO suspension were investigated, photocatalytic H2 evolution efficiency from the The experimental results indicate that ZnO rianoparticles calcined at 400℃ exhibit the best photoactivity for the H2 production in comparison with the samples calcined at 300 and 500℃, and the photoeatalytie H2 production efficiency from a methanol solution is much higher than that from a triethanolamine solution. It can be ascribed to the oxidization of methanol also contributes to the H2 production during the photochemical reaction process. Moreover, the photocatalytic mechanism for the H2 production from the present ZnO suspension system containing methanol solution is also discussed in detail. 展开更多
关键词 Photocatalytic hydrogen production ZnO Photocatalytic activity Sacrificial reagent
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改良“Trizol Reagent”试剂提取总RNA方法 被引量:1
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作者 李少玲 袁青 高英茂 《山东医科大学学报》 2001年第3期196-196,共1页
关键词 Trizo reagent 异硫氢酸胍 RNA 提取
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改良的Ellman's法测定小鼠肝脏还原型谷胱甘肽含量 被引量:1
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作者 黎煌久 陈焕昭 刘幸平 《汕头大学医学院学报》 2000年第2期16-16,19,共2页
目的 :观察辅酶Q(CoQ10 )对急性肝损伤的保护作用。方法 :采用改良的Ellman s法测定小鼠肝脏还原型谷胱甘肽(GSH)的含量。结果 :CoQ10 高、低剂量组 ,肝脏GSH含量均较生理盐水 (NS)对照组高 ,其差异有显著和极显著性意义 (P <0 0 5... 目的 :观察辅酶Q(CoQ10 )对急性肝损伤的保护作用。方法 :采用改良的Ellman s法测定小鼠肝脏还原型谷胱甘肽(GSH)的含量。结果 :CoQ10 高、低剂量组 ,肝脏GSH含量均较生理盐水 (NS)对照组高 ,其差异有显著和极显著性意义 (P <0 0 5和P <0 0 1)。结论 :CoQ10 能减少超量醋氨酚 (AAP) 展开更多
关键词 谷胱甘肽 ellman's 辅酶Q10 急性肝损伤
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用Lipofectin Reagent转染猪子宫上皮细胞 被引量:1
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作者 吴惠贤 《畜牧兽医学报》 CAS CSCD 北大核心 1993年第2期175-180,共6页
本文用Lipofectin Reagent作为转染细胞的间型脂质,使Liposome与SV40 ts A209 DNA形成一种混合物转染猪子宫上皮细胞。此法比用磷酸钙和二乙氨乙基葡聚糖转染细胞的方法简单,高效、重复性好。转染后的猪子宫上皮细胞形状和大小略有改变... 本文用Lipofectin Reagent作为转染细胞的间型脂质,使Liposome与SV40 ts A209 DNA形成一种混合物转染猪子宫上皮细胞。此法比用磷酸钙和二乙氨乙基葡聚糖转染细胞的方法简单,高效、重复性好。转染后的猪子宫上皮细胞形状和大小略有改变;对温度敏感,用相同培养液培养7天,在33℃条件下细胞的生长速度是在40℃时的5倍. 展开更多
关键词 子宫 上皮细胞 转染 DNA
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Ellman射频刀在唇腺手术中的应用 被引量:1
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作者 李岩 于晓萍 王庆 《中国临床医学》 2013年第4期592-593,共2页
目的:比较Ellman射频刀与传统手术刀在唇腺手术中的手术效果。方法:将2012年9月—2013年2月在复旦大学附属中山医院行唇腺手术的60例患者随机分成Ellman射频刀组(n=32)和传统手术刀组(n=28)。分别评价2组患者手术时间、术后反应和术后... 目的:比较Ellman射频刀与传统手术刀在唇腺手术中的手术效果。方法:将2012年9月—2013年2月在复旦大学附属中山医院行唇腺手术的60例患者随机分成Ellman射频刀组(n=32)和传统手术刀组(n=28)。分别评价2组患者手术时间、术后反应和术后囊肿复发情况,并进行统计学分析。结果:传统手术刀组患者平均手术时间(24.75±3.39)min,Ellman射频刀组平均手术时间(14.97±3.51)min,差异有统计学意义(P<0.01)。术后1 d复诊,Ellman射频刀组患者黏膜肿胀和术后疼痛百分比分别为62.6%(20/32)、56.2%(18/32),明显低于传统手术刀组[100%(28/28)及82.1%(23/28)],(P均<0.01);术后1周复诊,患者均未复发黏液性囊肿。结论:与传统手术刀相比,应用Ellman射频刀进行唇腺手术安全可靠、手术可控性高、手术时间短、出血少、术后反应轻、囊肿复发率低。 展开更多
关键词 ellman射频刀 唇腺手术 复发
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Mechanisms of aid-leaching reagent calcium fluoride in the extracting vanadium processes from stone coal 被引量:23
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作者 Fei Wang Yi-Min Zhang +4 位作者 Jing Huang Tao Liu Yi Wang Xiao Yang Jie Zhao 《Rare Metals》 SCIE EI CAS CSCD 2013年第1期57-62,共6页
The mechanisms of aid-leaching reagent calcium fluoride in the extracting vanadium processes were researched by comparing the blank extraction with the extraction with calcium fluoride as aid-leaching reagent. The lea... The mechanisms of aid-leaching reagent calcium fluoride in the extracting vanadium processes were researched by comparing the blank extraction with the extraction with calcium fluoride as aid-leaching reagent. The leaching experiments were carried out under the conditions of 95 ~C, 6 h, 1.5 ml.g-1, 15 vol % H2SO4, and 5 % calcium fluoride (mass ratio of CaF2 to stone coal). The concentrations of hydrogen ion were adjusted from initial 1.82 and 4.79 mol.L-1 to the pH of 1.80 4- 0.05 by 200 g.L-1 lime milk, respectively. The solvent extraction experiments were carded out under the organic phase using 15 vol% D2EHPA, 5 vol% TBP, and 80 vol% sulfonated kerosene and O/A phase ratio of 1/4. The leaching rate of vanadium and six-stage countercurrent solvent extraction rate of vanadium can be remarkably improved from 66.71 to 92.97 % and 81.15 to 98.81%, respectively, when calcium fluoride was added as aid-leaching reagent. In the leaching process, fluorine can enhance the breakage of the structure of vanadium-bearing minerals. Meanwhile, fluoride ion can combine with iron ion to generate acid-insoluble iron fluoride hydrate (Fe2Fs.7H20) and consume all ferric ions and a portion of ferrous ions. In adjusting pH value process, the massive impurities are decreased to form KA1SO4 and FeA1Fs. In solvent extraction process, fluorine can prevent the formation of ferric hydroxide colloids and eliminate the formed unstable ferric hydroxide colloids and the silicon-containing colloids to promote the sufficient contact between the aqueous phase and the organic phase and the sufficient reaction. 展开更多
关键词 VANADIUM Stone coal Aid-leaching reagent Calcium fluoride MECHANISMS
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Exploitation of Fenton and Fenton-like reagents as alternative conditioners for alum sludge conditioning 被引量:27
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作者 Maha A. Tony Y. Q. Zhao Aghareed M. Tayeb 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2009年第1期101-105,共5页
The use of Fenton's reagent (Fe^2+/H2O2) and Fenton-like reagents containing transition metals of Cu(Ⅱ), Zn(Ⅱ), Co(Ⅱ), and Mn(Ⅱ) for an alum sludge conditioning to improve its dewaterability was invest... The use of Fenton's reagent (Fe^2+/H2O2) and Fenton-like reagents containing transition metals of Cu(Ⅱ), Zn(Ⅱ), Co(Ⅱ), and Mn(Ⅱ) for an alum sludge conditioning to improve its dewaterability was investigated. The results obtained were compared with those obtained from conditioning the same alum sludge using cationic and anionic polymers. Experimental results show that Fenton's reagent was the best among the Fenton and Fenton-like reagents for the alum sludge conditioning. A considerable effectiveness of capillary suction time (CST) reduction efficiency of 47% can be achieved under test conditions of Fe^2+/H2O2 = 20/125 mg/g DS (dry solid) and pH 6.0. The observation of floc-like particles after Fenton's reagent conditioning of alum sludge suggested that the mechanism of Fenton's reagent conditioning was different from that of polymer conditioning. In spite of the lower efficiency in the CST reduction of Fenton's reagent in alum sludge conditioning compared to that of polymer conditioning, Fenton's reagent offers a more environmentally safe option. Tiffs study provided an example of proactive treatment engineering, which is aimed at seeking a safe alternative to the use of polymers in sludge conditioning towards achieving a more sustainable sludge management strategy. 展开更多
关键词 alum sludge CONDITIONING Fenton and Fenton-like reagents organic polymers cost estimate
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Reagent types and action mechanisms in ilmenite flotation:A review 被引量:7
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作者 Jiaozhong Cai Jiushuai Deng +5 位作者 Liang Wang Mingzhen Hu Hongxiang Xu Xiaoan Hou Bozeng Wu Shimei Li 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2022年第9期1656-1669,共14页
Ilmenite is an essential mineral for the extraction of titanium.Conventional physical separation methods have difficulty recovering fine ilmenite,and dressing plants have begun applying flotation to recover ilmenite.T... Ilmenite is an essential mineral for the extraction of titanium.Conventional physical separation methods have difficulty recovering fine ilmenite,and dressing plants have begun applying flotation to recover ilmenite.The interaction of reagent groups with Ti and Fe sites on the ilmenite surface dramatically influences the ilmenite flotation.However,the investigation on Fe sites has received more attention because the activity of Ti is lower than that of Fe.For the activators on ilmenite flotation,most are metal ions but typically lead ions.The metal ions of activators promote ilmenite flotation by increasing the active sites on the ilmenite surface.Combined reagents have a better selective separation of ilmenite than single reagents due to their synergistic effect.Combining the lead ion(Pb^(2+))and the benzyl hydroxamic acid(BHA)into a Pb-BHA complex has a marked effect on ilmenite flotation,which puts forward a new idea of developing combined reagents for ilmenite flotation.This review considers reagent types and action mechanisms in ilmenite flotation.On the basis of the analysis of previous research,a brief future outlook of reagent types and action mechanisms in ilmenite flotation is also proposed in this study. 展开更多
关键词 ILMENITE FLOTATION reagent action mechanism combined reagents
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Diagnosis of spontaneous bacterial peritonitis: An update on leucocyte esterase reagent strips 被引量:16
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作者 Anastasios Koulaouzidis 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第9期1091-1094,共4页
Ascites remain the commonest complication of decompensated cirrhosis. Spontaneous bacterial peritonitis (SBP) is defined as the infection of ascitic fluid (AF) in the absence of a contiguous source of infection and/or... Ascites remain the commonest complication of decompensated cirrhosis. Spontaneous bacterial peritonitis (SBP) is defined as the infection of ascitic fluid (AF) in the absence of a contiguous source of infection and/or an intraabdominal inflammatory focus. An AF polymorphonuclear (PMN) leucocyte count ≥ 250/mm 3 -irrespective of the AF culture resultis universally accepted nowadays as the best surrogate marker for diagnosing SBP. Frequently the results of the manual or automated PMN count do not reach the hands of the responsible medical personnel in a timely manner. However, this is a crucial step in SBP management. Since 2000, 26 studies (most of them published as full papers) have checked the validity of using leukocyte esterase reagent strips (LERS) in SBP diagnosis. LERS appear to have low sensitivity for SBP, some LERS types more than others. On the other hand, though, LERS have consistently given a high negative predictive value (> 95% in the majority of the studies) and this supports the use of LERS as a preliminary screening tool for SBP diagnosis. Finally, an AF-tailored dipstick has been developed. Within the proper setting, it is set to become the mainstream process for handling AF samples. 展开更多
关键词 PERITONITIS Leukocytes ESTERASES DIAGNOSIS reagent strips BACTERIAL
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Optimization of efficient stable reagent of alkaline thiourea solution for gold leaching 被引量:5
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作者 王云燕 柴立元 +2 位作者 闵小波 何德文 彭兵 《Journal of Central South University of Technology》 EI 2003年第4期292-296,共5页
Na2SiO3 and Na2SeO3 were chosen as stable reagents of alkaline thiourea solution substituting Na2SO3, according to the structure-property relationship between the stability of alkaline thiourea and the structure of th... Na2SiO3 and Na2SeO3 were chosen as stable reagents of alkaline thiourea solution substituting Na2SO3, according to the structure-property relationship between the stability of alkaline thiourea and the structure of thiourea and sulfite ion, and the effect of the stable reagents on stability of alkaline thiourea was investigated. The results show that contrary to Na2SeO3, Na2SO3 and Na2SiO3 affect the stability of alkaline thiourea solution remarkably. The stable effect of Na2SiO3 on alkaline thiourea is obviously better than that of Na2SO3. The stable reagents Na2SO3 and Na2SiO3 decrease the decomposition rate of alkaline thiourea solution greatly, and the decomposition rate of alkaline thiourea reduces from (72.5%) to (33.8%) with addition of (0.3 mol·L-1) Na2SiO3. Dissolution currents of gold in the alkaline thiourea solution containing Na2SO3 and Na2SiO3 are (2.0) (mA·cm-2) and (3.5) (mA·cm-2) at the potential of 0.42 V, respectively, and Na2SO3 is consumed excessively due to the oxidation reaction of Na2SO3 occurring in the studied potential range. Na2SiO3 is an efficient stable reagent of alkaline thiourea solution, and gold dissolution is accelerated much more obviously by Na2SiO3 than by Na2SO3. 展开更多
关键词 ALKALINE THIOUREA gold LEACHING STABLE reagent NA2SIO3 Na2SO3 Na2SeO3
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Novel Synthetic Method for the Vilsmeier-Haack Reagent and Green Routes to Acid Chlorides, Alkyl Formates, and Alkyl Chlorides 被引量:12
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作者 Yoshikazu Kimura Daisuke Matsuura 《International Journal of Organic Chemistry》 2013年第3期1-7,共7页
An environmentally benign and practical preparation method for the Vilsmeier-Haack reagent (VH) has been developed by using phthaloyl dichloride with DMF in toluene or 2-chlorotoluene. Phthalic anhydride as the byprod... An environmentally benign and practical preparation method for the Vilsmeier-Haack reagent (VH) has been developed by using phthaloyl dichloride with DMF in toluene or 2-chlorotoluene. Phthalic anhydride as the byproduct was recovered in high yield by simple filtration. Some aromatic acids have been transformed into the corresponding acid chlorides in good yields by employing the isolated VH. Treatment of primary or secondary alcohols with VH gave alkyl formates or alkyl chlorides by depending on the reaction conditions. 展开更多
关键词 Phthaloyl DICHLORIDE Vilsmeier-Haack reagent ACID Chlorides ALKYL FORMATES ALKYL Chlorides
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Studies on New Additions to 5-Methoxy-2 (5H)-Furanone: 1, 4-Addition of Grignard Reagents, and 1,3-Dipolar Cycloaddition of Silyl Nitronates 被引量:3
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作者 Zhao Yang WANG Jia Ling CUI +1 位作者 Bao Shan DU Qing Hua CHEN 《Chinese Chemical Letters》 SCIE CAS CSCD 2001年第4期293-296,共4页
1,4-Addition reaction of Grignard's reagent to 5-methoxy-2(5H)-furanone 1 was accomplished. which provided a new possible route for synthesizing beta -alkyl-Y-alkyloxy-Y-butyrolactone. A novel concise method of pr... 1,4-Addition reaction of Grignard's reagent to 5-methoxy-2(5H)-furanone 1 was accomplished. which provided a new possible route for synthesizing beta -alkyl-Y-alkyloxy-Y-butyrolactone. A novel concise method of preparing fused heterocyclic compounds was offered by 1,3-dipolar cycloaddition reaction of silyl nitronates to 1. 展开更多
关键词 Michael addition Grignard reagent 1 3-dipolar cycloaddition silyl nitronates.
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Degradation of polyvinyl-alcohol wastewater by Fenton’s reagent: Condition optimization and enhanced biodegradability 被引量:12
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作者 肖羽堂 许双双 李志花 《Journal of Central South University》 SCIE EI CAS 2011年第1期96-100,共5页
The pretreatment of refractory polyvinyl-alcohol (PVA) wastewater with low value of CODcr by Fenton's reagent was investigated to enhance the biodegradabilily. The effects of operating conditions such as pH of the ... The pretreatment of refractory polyvinyl-alcohol (PVA) wastewater with low value of CODcr by Fenton's reagent was investigated to enhance the biodegradabilily. The effects of operating conditions such as pH of the solution, Fe2+ dosage, H2O2 dosage, reaction time and initial PVA concentration on the removal efficiency of CODCr were discussed. It is demonstrated that the optimum value of pH for removal of CODcr is 5 and the most suitable dosages of H2O2 (2%) and FeSO4 (10 mg/L) are 5% and 8.0%, respectively. When the initial CODcr value of the PVA water is 760 mg/L, the favorable reaction time is 110 min. Under these optimum conditions, the removal ratio of CODcr is 58.6% 61.4%, and the value of biodegradability (CODB/CODcr) increases markedly from 8.9% 9.7% to 62.6% 68.3%. 展开更多
关键词 Fenton's reagent POLYVINYL-ALCOHOL PRETREATMENT BIODEGRADABILITY
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Quantitative Correlation between Group Connectivity Index and Property of Flotation Reagent 被引量:2
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作者 Cao, YC Jiang, YR +1 位作者 Xue, YL Wang, DZ 《Journal of Central South University》 SCIE EI CAS 2000年第1期25-28,共4页
Topological method is applied firstly to calculate the group connectivity indexes of some flotation reagents for sulfide minerals and oxide minerals. The study reveals that some properties of flotation reagents, such ... Topological method is applied firstly to calculate the group connectivity indexes of some flotation reagents for sulfide minerals and oxide minerals. The study reveals that some properties of flotation reagents, such as group electronegativity, energy criterion, solubility product of chemicals and maximum wavelength of ultraviolet absorbency, have linear correlation with the first order group connectivity index (GCI) of polar group, and the related coefficients are all larger than 0.900. The GCI can be used to characterize the structure of groups, and is a sort of new effective structural parameter to study the quantitative structure activity relationship of flotation reagents. 展开更多
关键词 FLOTATION reagentS group connectivity INDEX PROPERTY QUANTITATIVE CORRELATION
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A novel method for the spectrophotometric determination of cefradine by using sodium nitroprusside as chromogenic reagent 被引量:2
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作者 Hua Zhang Ling Li Wu +1 位作者 Quan Min Li Xin Zhen Du 《Chinese Chemical Letters》 SCIE CAS CSCD 2008年第12期1470-1474,共5页
A novel method is developed for the determination of cefradine by using sodium nitroprusside as chromogenic reagent. The experiment indicates that a russety product is formed by the reaction of cefradine with sodium n... A novel method is developed for the determination of cefradine by using sodium nitroprusside as chromogenic reagent. The experiment indicates that a russety product is formed by the reaction of cefradine with sodium nitroprusside in basic solution, and the maximum absorption wavelength (λmax) of russety product is 505 rim. And the sensitization of tetradecyl benzyl dimethyl ammonium chloride for the reaction of cefradine with sodium nitroprusside is remarkable, The apparent molar absorption coefficient (5505) is 2.81 × 103 L/mol cm. The linear equation isA = 0.0657 + 0.00804C (μg/mL) in the range of 1.50-55.0μg/mL of cefradine with a correlation coefficient r = 0.9992, and the detection limit is 1.38 p,g/mL. This method has been applied to determine cefradine in capsule and tablet samples. 展开更多
关键词 CEFRADINE Sodium nitroprusside Chromogenic reagent SPECTROPHOTOMETRY
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