An embryo classification system for Pinus tabuliformis Carr. was established by time-tracing sam- pling and observation of zygotic embryos. The zygotic embryos were divided into nine stages. Key elements of the zygoti...An embryo classification system for Pinus tabuliformis Carr. was established by time-tracing sam- pling and observation of zygotic embryos. The zygotic embryos were divided into nine stages. Key elements of the zygotic embryo and female gametophyte （FG） tissue of P. tabuliformis were analyzed, using inductively coupled plasma-emission spectroscopy. Several elements--includ- ing aluminum, iron, sodium, and copper--are found in both embryo and FG tissue. Boron, phosphorus, magnesium,zinc, and calcium are also required for zygotic embryo development and therefore accumulated. Manganese is selectively excluded from the embryo. The zygotic embryo development needs a low-sodium and high-potassium nutrition proportion. The results of elemental analysis from zygotic embryos and FGs can provide the mineral targets for optimizing the formulation of culture medium for somatic embryogenesis.展开更多
基金financially supported by an open fund of the Key Laboratory of Forest Genetics and BiotechnologyMinistry of Education and Jiangsu Province+3 种基金Nanjing Forestry University(FGB200901)the‘‘948’’project of China(2014-4-59)the Project of National Natural Science Foundation of China(No.31370658)‘‘the Mutual Fund of Beijing Government and Central Universities in Beijing(GJ2011-2)’’
文摘An embryo classification system for Pinus tabuliformis Carr. was established by time-tracing sam- pling and observation of zygotic embryos. The zygotic embryos were divided into nine stages. Key elements of the zygotic embryo and female gametophyte （FG） tissue of P. tabuliformis were analyzed, using inductively coupled plasma-emission spectroscopy. Several elements--includ- ing aluminum, iron, sodium, and copper--are found in both embryo and FG tissue. Boron, phosphorus, magnesium,zinc, and calcium are also required for zygotic embryo development and therefore accumulated. Manganese is selectively excluded from the embryo. The zygotic embryo development needs a low-sodium and high-potassium nutrition proportion. The results of elemental analysis from zygotic embryos and FGs can provide the mineral targets for optimizing the formulation of culture medium for somatic embryogenesis.
