Cryopreservation is the main functional means for storage of excess embryos produced from artificial reproductive technologies;the process assumes embryos chemical nature is highly conserved across embryos of the same...Cryopreservation is the main functional means for storage of excess embryos produced from artificial reproductive technologies;the process assumes embryos chemical nature is highly conserved across embryos of the same species. However, in practice there appears to be a high degree of variability in embryo tolerance to cryopreservation, suggesting potential differences in embryo chemistry. The objective of the current study was to develop reproducible means of estimating relative embryos weight and associating those weights to lipid content. Relative embryo weights of frozen/thawed in-vivo Jersey and Crossbred beef breed embryos were determined using a modified specific gravity chamber. Embryo weights were then correlated with lipid content. Results suggest that beef cattle embryos are 20% - 27% heavier than Jersey embryos展开更多
There can be little argument that embryo quality is one of the most critical factors in the success of assisted reproductive techniques. Yet the current methods of grading embryos are subjective at best. While a numbe...There can be little argument that embryo quality is one of the most critical factors in the success of assisted reproductive techniques. Yet the current methods of grading embryos are subjective at best. While a number of different groups have described more qualitative means of assessing embryo quality, the current standard remains morphology. Morphology has proven a good standard, but it does not allow for the detection of chromosomal abnormalities nor can it assess the biochemical status of the embryo prior to transfer. This laboratory recently described a method to estimate embryo weight and suggested weight might be a good indicator of biochemical status. The objective of the present study was to determine the relationship between maternal body composition and embryo weight and determine the influence maternal chemistry had on embryo development. The data continue to suggest that maternal body composition, especially body fat, influences the chemical nature of the embryo and may play a critical role in long-term survival.展开更多
Summary： Whether the type of culture media utilized in assisted reproductive technology has impacts on laboratory outcomes and birth weight of newborns in in-vitro fertilization （IVF）/intracytoplasmic sperm injecti...Summary： Whether the type of culture media utilized in assisted reproductive technology has impacts on laboratory outcomes and birth weight of newborns in in-vitro fertilization （IVF）/intracytoplasmic sperm injection （ICSI） was investigated. A total of 673 patients undergoing IVF/ICSI and giving birth to live singletons after fresh embryo transfer on day 3 from Jan. 1, 2010 to Dec. 31, 2012 were included. Three types of culture media were used during this period： Quinn＇s Advantage （QA）, Single Step Medium （SSM）, and Continuous Single Culture medium （CSC）. Fertilization rate （FR）, normal fertilization rate （NFR）, cleavage rate （CR）, normal cleavage rate （NCR）, good-quality embryo rate （GQER） and neonatal birth weight were compared using one-way ANOVA and Z2 tests. Multiple linear regression analysis was performed to determine the impact of culture media on laboratory outcomes and birth weight. In IVF cycles, GQER was significantly decreased in SSM medium group as compared with QA or CSC media groups （63.6% vs. 69.0% in QA; vs. 71.3% in CSC, P=0.011）. In ICSI cycles, FR, NFR and CR were significantly lower in CSC medium group than in other two media groups. No significant difference was observed in neonatal birthweight among the three groups （P=0.759）. Multiple linear regression analyses confirmed that the type of culture medium was correlated with FR, NFR, CR and GQER, but not with neonatal birth weight. The type of culture media had potential influences on laboratory outcomes but did not exhibit an impact on the birth weight of singletons in ART.展开更多
The effects of thyroxine and atropine in ameliorating phosphamidon intoxication in chick embryos was studied. Treatment of phosphamidon significantly enhanced the moriality and abnormalityrates, decreased the average ...The effects of thyroxine and atropine in ameliorating phosphamidon intoxication in chick embryos was studied. Treatment of phosphamidon significantly enhanced the moriality and abnormalityrates, decreased the average body weights, and cholinesterase activity in chick embryos. When thyroxine was administered to the phosphamidon intoxicated embryos, the above parameters changedsignificantly, indicating an ameliorating effect of thyroxine against phosphamidon intoxication in chick embryos. The combined thyroxine and atropine therapy did not further improve the ameliorating effect. Since in many respects chick embryo development parallels that of mammalian embryos,a short-term use of thyroxine as a protective agent against organophosphate toxicity might be useful展开更多
目的:系统评价低分子肝素联合黄体酮治疗胚胎移植患者反复种植失败(RIF)的有效性和安全性,为其临床应用提供参考。方法:计算机检索Cochrane图书馆、PubMed、Embase、中国知网和万方数据库,收集低分子肝素联合黄体酮(试验组)对比黄体酮(...目的:系统评价低分子肝素联合黄体酮治疗胚胎移植患者反复种植失败(RIF)的有效性和安全性,为其临床应用提供参考。方法:计算机检索Cochrane图书馆、PubMed、Embase、中国知网和万方数据库,收集低分子肝素联合黄体酮(试验组)对比黄体酮(对照组)用于RIF患者的随机对照试验(RCT)或观察性研究,检索时限为各数据库建库起至2020年7月。对符合纳入标准的研究进行资料提取,并采用Cochrane 5.1.0偏倚风险评估工具和Newcastle-Ottawa Scale评价量表分别对RCT和观察性研究进行质量评价。采用Rev Man 5.3软件进行Meta分析。结果:共纳入15项研究,包括10项RCT和5项观察性研究,合计2353例患者。Meta分析结果显示,试验组患者的着床率[RR=1.41,95%CI(1.24,1.60),P<0.01]、妊娠率[RR=1.38,95%CI(1.23,1.56),P<0.01]、活产率[RR=1.41,95%CI(1.19,1.67),P<0.01]均显著高于对照组,差异均有统计学意义;流产率[RR=0.54,95%CI(0.33,0.86),P=0.01]显著低于对照组,差异有统计学意义。亚组分析结果显示,年龄≥35岁患者着床率[RR=2.38,95%CI(1.31,4.31),P=0.004]、妊娠率[RR=2.58,95%CI(1.62,4.11),P<0.001]均高于对照组,差异均有统计学意义。两组患者不良反应主要是少量出血和注射部位瘀斑,未见严重不良反应。结论:低分子肝素联用黄体酮可提高RIF患者的胚胎着床率、妊娠率和活产率,降低其流产率,且安全性较好。展开更多
文摘Cryopreservation is the main functional means for storage of excess embryos produced from artificial reproductive technologies;the process assumes embryos chemical nature is highly conserved across embryos of the same species. However, in practice there appears to be a high degree of variability in embryo tolerance to cryopreservation, suggesting potential differences in embryo chemistry. The objective of the current study was to develop reproducible means of estimating relative embryos weight and associating those weights to lipid content. Relative embryo weights of frozen/thawed in-vivo Jersey and Crossbred beef breed embryos were determined using a modified specific gravity chamber. Embryo weights were then correlated with lipid content. Results suggest that beef cattle embryos are 20% - 27% heavier than Jersey embryos
文摘There can be little argument that embryo quality is one of the most critical factors in the success of assisted reproductive techniques. Yet the current methods of grading embryos are subjective at best. While a number of different groups have described more qualitative means of assessing embryo quality, the current standard remains morphology. Morphology has proven a good standard, but it does not allow for the detection of chromosomal abnormalities nor can it assess the biochemical status of the embryo prior to transfer. This laboratory recently described a method to estimate embryo weight and suggested weight might be a good indicator of biochemical status. The objective of the present study was to determine the relationship between maternal body composition and embryo weight and determine the influence maternal chemistry had on embryo development. The data continue to suggest that maternal body composition, especially body fat, influences the chemical nature of the embryo and may play a critical role in long-term survival.
文摘Summary： Whether the type of culture media utilized in assisted reproductive technology has impacts on laboratory outcomes and birth weight of newborns in in-vitro fertilization （IVF）/intracytoplasmic sperm injection （ICSI） was investigated. A total of 673 patients undergoing IVF/ICSI and giving birth to live singletons after fresh embryo transfer on day 3 from Jan. 1, 2010 to Dec. 31, 2012 were included. Three types of culture media were used during this period： Quinn＇s Advantage （QA）, Single Step Medium （SSM）, and Continuous Single Culture medium （CSC）. Fertilization rate （FR）, normal fertilization rate （NFR）, cleavage rate （CR）, normal cleavage rate （NCR）, good-quality embryo rate （GQER） and neonatal birth weight were compared using one-way ANOVA and Z2 tests. Multiple linear regression analysis was performed to determine the impact of culture media on laboratory outcomes and birth weight. In IVF cycles, GQER was significantly decreased in SSM medium group as compared with QA or CSC media groups （63.6% vs. 69.0% in QA; vs. 71.3% in CSC, P=0.011）. In ICSI cycles, FR, NFR and CR were significantly lower in CSC medium group than in other two media groups. No significant difference was observed in neonatal birthweight among the three groups （P=0.759）. Multiple linear regression analyses confirmed that the type of culture medium was correlated with FR, NFR, CR and GQER, but not with neonatal birth weight. The type of culture media had potential influences on laboratory outcomes but did not exhibit an impact on the birth weight of singletons in ART.
文摘The effects of thyroxine and atropine in ameliorating phosphamidon intoxication in chick embryos was studied. Treatment of phosphamidon significantly enhanced the moriality and abnormalityrates, decreased the average body weights, and cholinesterase activity in chick embryos. When thyroxine was administered to the phosphamidon intoxicated embryos, the above parameters changedsignificantly, indicating an ameliorating effect of thyroxine against phosphamidon intoxication in chick embryos. The combined thyroxine and atropine therapy did not further improve the ameliorating effect. Since in many respects chick embryo development parallels that of mammalian embryos,a short-term use of thyroxine as a protective agent against organophosphate toxicity might be useful
文摘目的:系统评价低分子肝素联合黄体酮治疗胚胎移植患者反复种植失败(RIF)的有效性和安全性,为其临床应用提供参考。方法:计算机检索Cochrane图书馆、PubMed、Embase、中国知网和万方数据库,收集低分子肝素联合黄体酮(试验组)对比黄体酮(对照组)用于RIF患者的随机对照试验(RCT)或观察性研究,检索时限为各数据库建库起至2020年7月。对符合纳入标准的研究进行资料提取,并采用Cochrane 5.1.0偏倚风险评估工具和Newcastle-Ottawa Scale评价量表分别对RCT和观察性研究进行质量评价。采用Rev Man 5.3软件进行Meta分析。结果:共纳入15项研究,包括10项RCT和5项观察性研究,合计2353例患者。Meta分析结果显示,试验组患者的着床率[RR=1.41,95%CI(1.24,1.60),P<0.01]、妊娠率[RR=1.38,95%CI(1.23,1.56),P<0.01]、活产率[RR=1.41,95%CI(1.19,1.67),P<0.01]均显著高于对照组,差异均有统计学意义;流产率[RR=0.54,95%CI(0.33,0.86),P=0.01]显著低于对照组,差异有统计学意义。亚组分析结果显示,年龄≥35岁患者着床率[RR=2.38,95%CI(1.31,4.31),P=0.004]、妊娠率[RR=2.58,95%CI(1.62,4.11),P<0.001]均高于对照组,差异均有统计学意义。两组患者不良反应主要是少量出血和注射部位瘀斑,未见严重不良反应。结论:低分子肝素联用黄体酮可提高RIF患者的胚胎着床率、妊娠率和活产率,降低其流产率,且安全性较好。
