Experimental Study of Emilia Sonchifolia Combined with Coptidis Rhizoma for the Prevention and Treatment of Oral Ulcer

Objective: This paper aims to investigate the therapeutic effect of the combination of Emilia Sonchifolia and Coptidis Rhizoma on oral ulcer rats. Methods: 36 SD rats of half male and half female were kept for 7 days, and 6 rats among them were selected as normal group by random sampling method, and the rest rats were randomly divided into model group, positive control group, Emilia Sonchifolia group, Coptidis Rhizoma group and combined group after the establishment of oral ulcer model. The normal group and model group were given blank film, the positive control group was given Guilin Watermelon Frost, and the Emilia Sonchifolia group, Coptidis Rhizoma group and combined group were given the corresponding oral film, which was administered to the ulcer for 7 days continuously, 2 times per day. The healing of oral ulcer was assessed at the end of the last day of administration, and the ulcer area was calculated on the 1st, 3rd, 5th and 7th days after successful modeling, and the serum levels of IL-2 and TNF-α in rats were detected by Emilia Sonchifolia. Results: The grading of ulcer healing in the positive control group and the combined group was better than the other groups, and the difference was statistically significant when compared with the model group (p Coptidis Rhizoma group, Emilia Sonchifolia group, combined group and positive control group was smaller than that in the model group, and the difference was statistically significant (p Emilia Sonchifolia group, Coptidis Rhizoma group and combined group were all effective in reducing the area of oral ulcer, among which the combined group was more effective. The level of pro-inflammatory factor TNF-α was reduced and the level of anti-inflammatory factor IL-2 was increased in the Emilia Sonchifolia group, Coptidis Rhizoma group and combined group, and the difference was statistically significant (p Conclusion: The combination of Coptidis Rhizoma and Emilia Sonchifolia is effective in the treatment of oral ulcer in rats, and the effect of the combination is better than that of the drug alone.

Effect of Emilia sonchifolia(Linn.)DC on alcohol- induced oxidative stress in pancreas of male albino rats

Objective:To explore the efficacy of n-hexane extract of Emilia sonchifolia(E.sonchifobia) against ethanol induced pancreatic dysfunction in the young Wistar albino rats.Methods:The rats were divided into four groups.Control rats in group I received distilled water orally,groupⅡreceived oral administration of 20%(w/v) ethanol dissolved in drinking water,groupⅢreceived oral administration of 20%(w/v) ethanol in distilled water+n-hexane extract of E.sonchifolia (250 mg/kg body weight),and groupⅣreceived oral administration of n-hexane extract of E.sonchifolia(250 mg/kg body weight) alone.Liver marker enzymes aspartate aminotransferase (AST),alanine aminotransferase(ALT),pancreatic enzymatic antioxidants superoxide dismutase. lipid peroxidation,catalase,glutathione peroxidase,non-enzymatic antioxidants glutathione and vitamin C were measured and compared.Results:Administration of 20%ethanol lor 16 weeks significantly increased the liver marker enzymes AST,ALT(P【0.05).reduced the pancreatic enzymatic antioxidants superoxide dismutase.lipid peroxidation,catalase,glutathione peroxidase,glutathione and vitamin C(P【0.05).Histopathological examination showed that the ethanol provoked the oxidative stress which was demonstrated as pancreatic necrosis and oedema.Simultaneous administration of n-hexane extract of E.sonchifolia(250 mg/kg body weight) protected the pancreas against the damage induced by ethanol which was confirmed by the histopathological studies and the normalization of biochemical parameters.Conclusions: Thus n-hexane extract of E.sonchifolia shows a promise in therapeutic use in alcohol induced oxidative stress.

Optimization of the Ultrasonic-assisted Extraction Process for Protocatechuic Acid from Emilia sonchifolia DC by Response Surface Methodology

[Objectives] This study was conducted to optimize the extraction process of protocatechuic acid from Emilia sonchifolia DC. [Methods] The optimal extraction conditions were determined by single factor,response surface analysis and variance analysis,and the content of protocatechuic acid was determined by HPLC. [Results] The protocatechuic acid standard curve equation was: y = 1 435 x + 8 403,R^2= 0. 999 8,indicating a good linear relationship. The optimal extraction conditions were as follows: a temperature at 80 ℃,an extraction time of 1 h,a material-to-liquid ratio at 1:10 and an ultrasonic power of 600 W,and the content of protocatechuic acid extracted was 1. 93 mg/g. The method showed a RSD of 0. 41%,less than 2%,and the detection limit was 0. 0000047261 g/ml.The experimental sample X1 was the low-level 0. 1 mg/ml standard solution,which showed recovery of protocatechuic acid between 100.8% and 105.2%,with a RSD of 0. 013%;and the sample X2 was the high-level 1. 0 mg/ml standard solution,which exhibited recovery between 100. 6% and 102. 2%,with a RSD of 0.076%. Thus,the recovery was high,and the requirements of the performance index were met. [Conclusions] The detection method is stable and reliable and can produce satisfactory results.

Sand Liquefaction Phenomena During the Seismic Crisis of May 2012 in Emilia,Northern Italy

In May 2012,the Emilia region of the Po Valley was struck by a seismic crisis with two major events of magnitude M_w 6.1and M_w 5.9.The first event induced widespread sand blows formed along buried channels and old crevasse splay deposits.In the days immediately following the events,the detailed mapping and sampling of the erupted sand was fundamental to record all the seismically-induced phenomena.The study of a trench dug across large fractures at San Carlo(Ferrara)provided also valuable information on the sand blows mechanism and regome.The sedimentological and compositional characteristics of the fracture-filling materials indicate that the sands were erupted from a layer located between 6.8 and 7.5 m depth.Older and deeper Holocene and Pleistocene sand layers were not apparently involved in the liquefaction phenomena.

Computational Stimulation and Experimental Study on Corrosion Inhibition Qualities of <i>Emilia sonchifolia</i>Leaf Extract for Copper (CU131729) in Hydrochloric Acid

Copper corrosion inhibition by Emilia sonchifolia (ES) leaf extract has been studied in 2 M hydrochloric acid solution using electrochemical measurement, energy dispersive X-ray emission spectroscopy and surface examination techniques. Computational simulations were adopted to describe probable reactiveness of individual ES leaf constituents and mechanism of interaction with copper crystal. Results obtained from potentiodynamic polarization revealed a shift in corrosion potentials of copper (CU131729) to more positive potentials in the presence of ES leaf extract with increased effect as inhibitor concentration increased and also a decrease in both current densities suggesting a mixed type inhibitor characteristics with pronounced anodic protection. Surface elemental characterization revealed presence of the inhibitor species in the corrosion products formed on corroded CU131729 surface confirming the formation of complex chelating ligands through interactions with the metal surface. Quantum chemical calculations and molecular dynamics simulations were employed to theoretically analyse the interactions of individual ES leaf constituent with copper surface at the molecular level and obtained results revealed strong and spontaneous adsorption with high binding energies which affirms observed quality inhibition action. Quantum chemical descriptors such as energy of HOMO and LUMO, energy gap, number of transferred electrons, global hardness and softness, electrophilicity, and interaction energy were computed and discussed.

幻灯片 西班牙Sra. Emilia办公室

Sra.Emilia广告公司位于西班牙,他们的新办公室由Erbalunga estudio为其打造而成。项目旨在为这个广告公司设计一个既适合商业活动,又能激发员工创意的办公环境。既有的空间较为狭窄,西侧是巨大的玻璃立面,东侧是两扇宽阔的窗户.

不同烹饪方式对一点红抗氧化活性的影响

为了探究不同烹饪方式对一点红抗氧化活性的影响,分别采用9种不同烹饪方式(漂烫0.5 min、漂烫1.5 min、漂烫2.5 min、煮制5 min、煮制10 min、煮制15 min、油炒2 min、油炒4 min、油炒6 min)对其处理后测定总多酚含量、总黄酮含量、DPPH清除率、总抗氧化能力、维生素C含量、叶绿体色素含量等指标。结果表明,总多酚含量最高、总抗氧化能力最强、叶绿素含量、类胡萝卜素含量最高的烹饪方式是油炒6 min;总黄酮含量、DPPH清除率最高的是漂烫2.5 min;维生素C含量最高的是漂烫0.5 min。主成分综合分析结果显示,综合得分排名最高的是油炒6 min。因此,油炒6 min是保留一点红最高抗氧化活性的最佳烹饪方式。

Acute and Prolonged Effects of a Polyherbal Formulation on Blood Glucose, Lipid Profile and Liver Function in Normal and Streptozotocin-Induced Diabetic Rats

Background: Few studies have often focused on medicinal plant mixtures, yet the most used in low-and middle-income areas as alternative drug to treat diseases. Objective: To evaluate the antidiabetic effects of Emilia coccinea (Ec) (Asteraceae), Scoparia dulcis (Sd) (Plantaginaceae) and Tetracarpidium conophorum (Tc) (Euphorbiaceae) aqueous extracts mixture (EcSdTc) in rats. Methodology: Single plant aqueous extracts (Ec, Sd and Tc) and their mixtures (EcSd, EcTc, SdTc and EcSdTc) (each at the doses of 125 and 250 mg/kg body weight respectively) were evaluated in acute administration on blood glucose in normal, glucose-overloaded and diabetic rats;then EcSdTc mixture was assessed in prolonged administration (21 days) on blood glucose, body weight, serum biochemical and antioxidant parameters in diabetic rats. Diabetes was induced by single intraperitoneal administration of streptozotocin (STZ;50 mg/kg), and glibenclamide (10 mg/kg) was used as standard drug. Results: In acute administration, EcTc250, EcSdTc125, SdTc250, SdTc125, EcSd250, and EcSdTc250 extracts mixtures reduced (p Conclusion: EcSdTc aqueous extracts mixture has potent hypoglycemic and antidiabetic effects, probably due to their bioactive compounds synergistic and/or additive actions, justifying its traditional use as alternative remedies.

幼儿园“会说话的墙”创设路径--基于瑞吉欧环境观

墙面环境具有较大的教育意义,其创设的质量关系到幼儿身心发展。瑞吉欧环境创设因注重师幼及家长地位的平等性、主题表征的丰富性、环境更新的及时性、幼儿活动记录的意义性等,一直受到广泛的关注。目前,我国幼儿园墙面环境的创设过程仍存在着现实问题,而借鉴瑞吉欧环境创设理念有助于幼儿园摆脱创设主体地位不平等、主题单调且更新周期长以及幼儿创造表征遭遮蔽等困境。探寻幼儿园“会说话的墙”创设的路径,可从建立平等关系、丰富墙面主题、关注幼儿经验等方面切入。

一点红黄酮成分的分析及含量测定

对一点红中黄酮类成分进行了定性分析和定量测定,结果表明,在一点红中含有黄酮、黄酮醇、二氢黄酮、二氢黄酮醇等多种黄酮类化合物,并以芦丁为标样,用分光光度法测出总黄酮含量为4.02%.

一点红黄酮类化合物分离及其抗菌性能研究

本实验采用70%的乙醇水溶液回流提取一点红中的黄酮类化合物,然后通过石油醚萃取除掉其中色素等杂质。提取物进一步通过聚酰胺薄板进行薄层色谱分析[展层剂为正丁醇:水:醋(10:5:1)],且通过聚酰胺柱进行分离纯化(洗脱液为70%乙醇水溶液)。薄层色谱分析结果(以芦丁标样为对照)以及相关的黄酮化学检识实验结果表明,一点红黄酮类化合物具有与芦丁成分相似的黄酮类化合物和较高的纯度,对金黄色葡萄球菌具有较强的抑菌作用,对大肠杆菌及枯草杆菌的抑菌效果次之。

一点红总黄酮的提取及对羟自由基的清除作用研究

目的探讨一点红总黄酮的提取、鉴别及对羟自由基清除作用,以充分利用一点红植物资源,避免资源的浪费。方法采用超声波乙醇浸提法从一点红中提取黄酮类物质,对所提取的黄酮类物质进行验证,并用分光光度法测定含量,用一点红总黄酮对羟自由基清除作用进行试验。结果测得样品中总黄酮的含量C=0.816 8 mg/ml,回收率为100%,其纯度和产率均较高。结论该方法采用全物理过程,无任何污染,是提取一点红黄酮类物质的有效途径。一点红总黄酮提取液对Fenton体系产生的.OH自由基有很好的清除作用。

广西一点红总生物碱的提取和抗菌活性研究

目的考察广西一点红总生物碱的分离提纯及其抗菌活性。方法通过95％乙醇回流提取一点红药材中的生物碱类物质，利用酸水提取法及有机溶剂萃取对其进行分离纯化，同时用化学检识法与薄层色谱法进行定性鉴别。采用滤纸片法研究一点红生物碱类物质的抗菌活性。结果一点红生物碱浓度在600～800mg／ml时对金黄色葡萄球菌、大肠杆菌及枯草杆菌中度敏感，随着药物（生物碱）浓度的增大，抗菌作用越明显。结论说明一点红生物碱类物质对供试菌有一定的抑菌活性。

广西传统中药一点红总黄酮含量分析

目的建立一点红总黄酮的含量测定方法，考察一点红原药材质量的优劣。方法利用正交设计法考察最佳提取条件，采用紫外-可见分光光度法，以芦丁为对照品，于510nm波长处测定总黄酮含量。结果芦丁在0．0312～0．0832mg／ml范围内呈良好线性关系（R^2=0．9984），平均加样回收率为96．8％，RSD=1．21％（n=6）。3批样品总黄酮平均含量为9．918mg／g。结论该法重复性较好，对仪器要求不高，准确可靠，可用于一点红质量控制。

一点红化学成分的研究

从一点红全草中分得5个化合物,经化学和光谱方法鉴定为simiral,β-谷甾醇,豆甾醇,棕榈酸和蜂蜜酸。

一点红黄酮分离及抗氧化研究

研究一点红黄酮的分离提纯及其清除1,1-二苯基-2-苦基苯肼自由基(DPPH·)和羟基自由基(·OH)作用。经乙醇回流提取、聚酰胺柱层析分离提纯得到一点红黄酮;采用薄层层析对其进行定性分析;采用紫外-可见分光光度法测定其清除DPPH·和·OH的能力。结果表明,一点红黄酮清除DPPH·和·OH的IC50分别是4.8×10-3、0.53mg/ml,说明它具有清除DPPH·和·OH的作用。

高效液相色谱法测定一点红中槲皮素的含量

目的建立高效液相色谱法测定一点红中槲皮素含量的方法。方法用Hypersil BDS C18色谱柱（4．6mm×200mm，5μm），流动相为甲醇-水-磷酸（55：45：0．4），检测波长为370nm。结果槲皮素在0．0416～0．416μg的范围内与峰面积呈良好线性关系（r=0．9998），平均加样回收率为102．3％，RSD为1．18％（n=6）。结论此法用于测定一点红中槲皮素的含量快速、简便、准确，为评价一点红的内在质量提供了科学依据。

一点红不同营养器官中总黄酮和总酚含量测定

目的:建立一点红不同营养器官中总黄酮和总酚含量测定方法。方法:以芦丁为对照品,采用NaNO2-Al(NO3)3-NaOH比色法测定了总黄酮含量;以没食子酸为对照品,采用Folin-Ciocalteu比色法测定了总酚含量。结果:芦丁浓度在0.01～0.08mg/ml范围内与吸光度呈良好线性关系,平均加样回收率为100.30%,RSD=1.72%,一点红叶、茎、根中总黄酮含量分别为2.54%、1.78%、1.27%;没食子酸浓度在0.002～0.010mg/ml范围内与吸光度呈良好线性关系,平均加样回收率为99.86%,RSD=1.92%,一点红叶、茎、根中总酚含量分别为21.28%、12.77%、8.51%。结论:这2种方法灵敏,准确性强,精密度高,重复性好,可以用作一点红中总黄酮和总酚含量的测定。

艾叶的营养成分分析

对艾叶的常规营养成分,VC、矿质元素及黄酮含量进行了分析测定,并与一点红进行了对比分析研究。研究结果表明:艾叶的灰分、蛋白质、粗纤维、矿质元素的含量都明显比一点红高;其中总糖是一点红的2.46倍,粗纤维是一点红的3.44倍,黄酮的含量是一点红2.94倍,Fe是一点红的2.59倍,Mn是一点红的1.47倍,Cu是一点红的2.52倍,Zn是一点红的2.11倍。

中药一点红致肝小静脉闭塞病1例并文献复习

肝小静脉闭塞病是一种病因复杂的少见病,国内报导多为服用土三七所致.本研究首次报导了一点红致肝小静脉闭塞病患者1例的临床诊治经过,并结合相关文献复习,提示临床医生对于应用一点红的患者,一旦出现腹胀、腹水、肝肿大、黄疸,应考虑肝小静脉闭塞病的发生,及时进行诊治.