多房棘球蚴囊泡来源EVs及其emu-miR-10-5p促进小鼠肝星状细胞活化

目的明确多房棘球绦虫囊泡来源胞外囊泡(Extracellular vesicles,EVs)及其emu-miR-10-5p在体外调节HSCs活化中的作用。方法小鼠腹腔注射原头节构建继发性多泡型包虫病模型,以小鼠体内病灶为材料培养多房棘球绦虫囊泡并进行PCR鉴定。透射电镜观察培养囊泡组织中胞外囊泡结构。差速-超速离心法分离囊泡培养上清中EVs,透射电镜、纳米粒径以及Western blot对分离EVs进行鉴定。CCK-8检测不同浓度EVs作用后HSCs活力。EVs作用于HSCs后RT-qPCR和Western blot检测α-SMA、CollagenⅠ和CollagenⅢ表达水平。生物信息学分析确定emu-miR-10-5p为特异性miRNA。过表达emu-miR-10-5p后EdU检测HSCs增殖,RT-qPCR个Western blot检测细胞中α-SMA、CollagenⅠ和CollagenⅢ表达水平。干扰囊泡组织中emu-miR-10-5p后提取培养上清液中EVs作用于HSCs后,检测HSCs细胞中α-SMA、CollagenⅠ和CollagenⅢ表达水平。结果成功通过小鼠体内病灶材料培养出多房棘球蚴囊泡,经PCR扩增特异性基因证实为多房棘球绦虫来源。TEM观察培养囊泡表面有较多的囊状结构。通过差速-超速离心分离出多房棘球蚴囊泡来源EVs,并经TEM证实为球形、膜状结构。囊泡来源EVs能被HSCs内化,并促进HSCs增殖和活化。通过综合分析多房棘球绦虫感染小鼠血清、棘球蚴组织、棘球蚴囊泡来源EVs、生发层细胞、原头节miRNA测序结果,发现emu-miR-10-5p在上述成分中均存在表达,并通过RT-qPCR证实emu-miR-10-5p在多房棘球蚴囊泡来源EVs中存在的量较高。进一步研究证实过表达emu-miR-10-5p能促进HSCs增殖,增加HSCs中α-SMA、Collagen I和CollagenⅢmRNA及蛋白表达水平。而干扰囊泡中emu-miR-10-5p后从培养上清液中提取的EVs未增加α-SMA、Collagen I和CollagenⅢmRNA及蛋白表达水平。结论多房棘球蚴囊泡来源EVs及emu-miR-10-5p能够促进HSCs活化。

Investigation on influencing factors of wheel polygonization of a plateau high-speed EMU train

Purpose–This study aims to investigate the cause of high-order wheel polygonization in a plateau high-speed electric multiple unit(EMU)train.Design/methodology/approach–A series of field tests were conducted to measure the vibration accelerations of the axle box and bogie when the wheels of the EMU train passed through tracks with normal rail roughness after re-profiling.Additionally,the dynamic characteristics of the track,wheelset and bogie were also measured.These measurements provided insights into the mechanisms that lead to wheel polygonization.Findings–The results of the field tests indicate that wheel polygonal wear in theEMUtrain primarily exhibits 14–16 and 25–27 harmonic orders.The passing frequencies of wheel polygonization were approximately 283–323 Hz and 505–545 Hz,which closely match the dominated frequencies of axle box and bogie vibrations.These findings suggest that the fixed-frequency vibrations originate from the natural modes of the wheelset and bogie,which can be excited by wheel/rail irregularities.Originality/value–The study provides novel insights into the mechanisms of high-order wheel polygonization in plateau high-speed EMU trains.Futher,the results indicate that operating the EMU train on mixed lines at variable speeds could potentially mitigate high-order polygonal wear,providing practical value for improving the safety,performance and maintenance efficiency of high-speed EMU trains.

Matlab-Simulink Simulation Model of Electric Multiple Unit(EMU)Series ŽS 413/417 in Traction and Electric Braking Mode

In the paper,the Matlab-Simulink model of simulation of operation of theŽS 413/417 series EMU(electric multiple unit)in traction and braking mode is exposed where changes are observed:stator currents of three-phase traction motors,traction electric motor speeds and EMU,electromagnetic torque on the rotor shaft of the traction electric motor and DC(direct current)bus voltage.The model allowed review of the listed parameters for:different allowed values of contact network voltage and total voltage distortion at the place of connection of the EMU to the contact network,different mechanical loads of EMU and traction electric motor and different train speeds and rotation of traction electric motors.Appropriate conclusions were made through the analysis of the simulation results obtained.

Customized High-speed EMU Design Practice for Jakarta-Bandung HSR

The high-speed EMUs for Jakarta-Bandung high-speed railway(HSR)represent an important image and symbol of the Jakarta-Bandung HSR Project.By relying on the technical platform of the model CR400 EMU,the EMU is upgraded for the customized design in terms of adaptability to geographical environment and track conditions,integration of Indonesian cultural characteristics,etc.In the course of development,in order to accurately grasp the boundary conditions and solve matching adaptation problems,studies are conducted on the exploration of design boundary and the related special technology.With the systematic analysis and design experience summary of the high-speed EMUs for Jakarta-Bandung HSR,the paper aims to provide reference for the O&M of high-speed EMUs for Jakarta-Bandung HSR and the other similar overseas HSR projects.

基于EMU8086的微机原理教学实践探索——以8086数据寻址方式为例

从微机原理课程特点及教学现状出发,分析传统教学方式的弊端。探讨了EMU8086在微机原理课程线上线下混合式教学模式中的应用,以8086的数据寻址方式的教学为例,介绍了EMU8086仿真软件的特点和功能,分析了8086数据寻址方式在该课程中的重要性。通过实际案例介绍如何使用EMU8086进行8086数据寻址方式的教学。实践表明,在混合式教学模式下,通过使用EMU8086辅助教学,学生可以更直观地理解8086处理器的工作原理和数据寻址方式,能够有效提高学生对微机原理的理解和实际操作能力。

An abnormal carbody swaying of intercity EMU train caused by low wheel-rail equivalent conicity and damping force unloading of yaw damper

Low-frequency carbody swaying phenomenon often occurs to railway vehicles due to hunting instability,which seriously deteriorates the ride comfort of passengers.This paper investigates low-frequency carbody swaying through experimental analysis and numerical simulation.In the tests,the carbody acceleration,the wheel-rail profiles,and the dynamic characteristics of dampers were measured to understand the characteristics of the abnormal carbody vibration and to find out its primary contributor.Linear and nonlinear numerical simulations on the mechanism and optimization measures were carried out to solve this carbody swaying issue.The results showed that the carbody swaying is the manifest of carbody hunting instability.The low equivalent conicity and the decrease of dynamic damping of the yaw damper are probably the cause of this phenomenon.The optimization measures to increase the equivalent conicity and dynamic damping of the yaw damper were put forward and verified by on-track tests.The results of this study could enrich the knowledge of carbody hunting and provide a reference for solving abnormal carbody vibrations.

Maintenance Optimization for EMU Trains Considering Environmental Impacts and Correlations

The complexity of the actual operating environment of EMU trains and the interaction between the reliability of system components have become a huge challenge for the maintenance scheduling of EMU trains. In response to these problems, the evolution of reliability and failure rate under the influence of environmental factors, failure correlations and economy correlations is analyzed. We assume bogie systems form the EMU train in series. The failure correlation matrix of the bogie systems is modeled. With the lowest total maintenance cost as the optimization objective, a decision-making model for EMU train maintenance is established. A dynamic maintenance strategy is proposed for the model, which can improve maintenance plans efficiently. Artificial bee colony algorithm is applied to further iteratively optimize the threshold parameters in the strategy. The results are calculated and verified by a numerical example. The results show the effectiveness of the maintenance decision model. The dynamic maintenance strategy in this paper is compared with the traditional opportunistic maintenance strategy. The proposed maintenance strategy outperforms the traditional opportunistic maintenance strategy in the numerical example.

Proteus和Emu8086在微机原理实验教学中的应用

微机原理是实践性强的课程。解决工程实践问题对改善微机原理教学和提高学生动手能力、创新能力具有重要意义。文中分析了目前微机原理教学中存在的问题,提出用Proteus和Emu8086构建联合仿真实验解决实践难的问题,以8086和8255A构建了交通灯仿真实例进行验证。仿真实验表明,构建的交通灯虚拟仿真实验可实现8086的模拟仿真,为解决微机原理实验困难和进行教学改革提供了一种新的方法。

EMU250转向架一系悬挂试验设计与研究

对一系悬挂进行力学性能试验是改善车辆动力学性能的重要手段。文章简要分析了EMU250转向架一系悬挂结构与原理,设计了一系悬挂组对滚动与滑动加载2种试验方案,重点分析了滚动加载试验方案,研究了一系悬挂垂向刚度和疲劳性能,结果表明一系悬挂垂向刚度呈正线性,疲劳次数对刚度影响较小,试验设计达到了预期效果,研究成果对轨道车辆一系悬挂的研发和试验起指导作用。

EMU8086软件在微机原理及接口技术教学中的应用

寻址方式的理论教学在微机原理及接口技术课程的教学过程中起着重要的作用,它是连接硬件和软件的纽带.在寻址方式的理论教学中通过EMU8086软件随堂演示各种寻址方式,即能激发学生的学习兴趣,又能让学生对寄存器、存储器、8086指令系统等产生感性认识,真正将软件和硬件结合.

Emu8086和Debug在《微机原理》课程教学中的应用

在微机原理教学中,可以使用Emu8086仿真软件和Debug软件进行汇编语言源程序的调试,通过两个实例就这两款软件的优缺点进行对比,便于读者有针对性地选择合适软件进行汇编语言程序的运行和调试。

EMU对欧盟经济的影响

在欧洲经济与货币一体化联盟(EMU)推动下,欧元已取代欧元区的各国货币,这对于欧盟经济和世界经济都构成巨大的影响。EMU对欧盟经济的影响,体现在宏观和微观两个层面,其中宏观性经济影响主要以相关的争论为主线,微观性经济影响主要考察对企业的影响效应。

EMU8086仿真软件在《微机原理》课程教学中的应用

针对微机原理教学中存在的问题,提出了用EMU8086仿真软件来解决的办法,并通过两个实例说明了该软件在汇编程序设计和接口技术方面的应用。

基于EMU8086的微机系统的辅助学习方法

描述了EMU8086的机理和基本功能,介绍了通过EMU8086学习汇编语言和微机接口技术的方法,对典型的虚拟接口实验及其操作方法进行了说明,归纳讨论了EMU8086在使用过程中可能遇到的一些问题。

EMU程序在吉音水利枢纽导流洞进口边坡处理中的应用

采用中国水利水电科学研究院开发的岩质边坡稳定分析程序EMU,对吉音水利枢纽工程导流洞进口右侧塌滑边坡选取典型剖面进行二维刚体极限平衡稳定分析,并进行有效处理,目前该处边坡工程已经正常运行5年。

基于Emu8086的汇编语言程序设计探讨

简单介绍Emu8086功能，通过汇编程序实例调试，对比Emu8086和Debug在汇编语言程序设计中的应用。可以看出，Emu8086的使用使得汇编语言程序的编译、执行变得更加直观和清晰，有助于提高初学者的学习兴趣。

基于Emu8086仿真的微机原理及接口技术教学整合的实践与探索

探讨了将Emu8086仿真技术与微机原理、接口技术课程教学有机结合。运用"主体-主导"的教学设计理念,合理利用Emu8086仿真软件进行设计教学,使学生直观、形象地理解微机结构及接口的工作原理和过程,提高学生的学习兴趣,激发学生的创造性和能动性,培养学生整体设计和开发能力,实现教学方式的转变。

Emu8086在汇编语言教学中的使用

在汇编语言实验教学过程中,传统的教学软件是Debug配合Masm来进行教学,但是使用这种搭配方式却存在诸多不足。通过讨论在汇编语言实验教学中所遇到的难点问题,提出如何利用Emu8086模拟器来解决。

EMU对欧盟经济的影响

在欧洲经济与货币一体化联盟 (EMU)的推动下 ,欧元已取代欧元区的各国货币 ,这对于欧盟经济和世界经济都构成巨大的影响。本文侧重研究EMU对欧盟经济的影响 ,对这种影响的分析将从宏观和微观两个层面展开 ,其中宏观性经济影响主要以相关的争论为主线 ,微观性经济影响主要考察对企业的影响效应。

基于Proteus和emu8086的交通灯设计与仿真

交通灯的智能管理是解决拥挤的城市交通,方便市民出行的重要举措。文章基于Intel 8086微处理器设计十字路口的智能交通灯控制系统,利用8086控制可编程并行接口芯片8255A驱动十字路口交通信号灯(红黄绿)的自动切换。本系统是基于proteus平台进行电路原理图的设计,利用emu8086进行程序的编写和调试。通过proteus和emu8086的联合调试和仿真,运行效果良好。