A predicted tau glutathione S-transferase(GST) subunit encoding gene,named GhGST,was isolated from Gossypium hirsutum with RACE method from SSH library based on Verticillium
Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ...Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ascidians,including those expressed in the larval tail muscle,the adult body-wall muscle,and adult heart muscle.In this study,a novel striated non-tail muscle actin gene was identified from the RNA-seq data of Ciona savignyi embryos.Phylogenetic analysis,alignment of the N-terminal amino acid sequences and comparation of diagnostic residues provided evidence that it had high similarity with vertebrate cardiac and skeletal muscle actin.In situ hybridization and promoter-driven GFP reporter assay revealed that it was specifically expressed in the primordia of the oral and atrial siphon.We hereby defined it as siphon-specific muscle actin coding gene(Cs-SMA).A 201 bp(−1350 bp to−1150 bp)sequence containing T-box and Six1/2 binding motif within the upstream region of Cs-SMA confined the expression of GFP in the siphons of electroporated embryos.Six1/2 binding motif was experimentally confirmed to play indispensable role in controlling the siphon-specific expression of Cs-SMA.The tissue-specific expression of Cs-SMA in the siphon primordia indicated its potential crucial roles in Ciona embryogenesis and organogenesis.展开更多
GPX-GI is a cytosolic tetrameric Se-dependent glutathione peroxidase, similar in properties to GPX-1. Unlike the almost ubiquitous GPX-1, GPX-GI is mainly expressed in the epithelium of gastrointestinal tract. GPX-GI ...GPX-GI is a cytosolic tetrameric Se-dependent glutathione peroxidase, similar in properties to GPX-1. Unlike the almost ubiquitous GPX-1, GPX-GI is mainly expressed in the epithelium of gastrointestinal tract. GPX-GI contributes to at least fifty percent of GPX activity in rodent small intestmal epithelium. The total GPX activity consists of at least 70% of selenium-dependent GPX activity in this compartment.By analyzing a panel of mouse mterspecies DNA from the Jackson Laboratory's backcross resource,we mapped Gpx2 gene to mouse chromosome 12 between D12Mit4 and D12Mit5, near the Ccs1 locus which contains a colon cancer susceptibility gene. A pseudogene, Gpx2-ps is mapped to mouse chromosome 7.Comparison of Gpx2 gene expression in three pairs of C57BL/6Ha and ICR/Ha mice which are respectively resistant and sensitive to dimethylhydrazine-induced colon cancer, we found a higher Gpx2 mRNA level in C57BL/6Ha colon than ICR/Ha colon. Interestingly, a lower level of GPX activity is found in the resistant strain of mice. Because GPX-1 has three times higher specific activity than GPX GI, our data suggest that the decreased GPX activity may result from a higher level of Gpx2 gene expression in those cells co-express GPx1 gene展开更多
High affinity phosphate transporterplays an important role in plantadapting to low phosphorus. Isolationof genes coding this kind of proteinhas attracted worldwide scholars toaccomplish. We aimed to isolate thegene an...High affinity phosphate transporterplays an important role in plantadapting to low phosphorus. Isolationof genes coding this kind of proteinhas attracted worldwide scholars toaccomplish. We aimed to isolate thegene and transfer it to target plants展开更多
The gene encoding urease subunit A (ureA) of Helicobacter pylori (H.pylori) was cloned from H.pylori isolate by polymerase chain reaction (PCR). Sterile distilled water instead of DNA served as negative control. The n...The gene encoding urease subunit A (ureA) of Helicobacter pylori (H.pylori) was cloned from H.pylori isolate by polymerase chain reaction (PCR). Sterile distilled water instead of DNA served as negative control. The nucleotide sequence of the amplified product was determined. Homologous analysis of the ureA against that reported by Clayton CL and the GenBank and SwissProt databases were performed with the BLAST program at the Genome Net through the Internet. 0.8 kb PCR product was amplified from all H.pylori clinical isolators. The nucleotide sequence of the ureA was determined. The nucleotide sequence of the ureA began with ATG as the initiation codon and terminated in TAA as stop codon. The coding regions had a 44 % G+C content. The DNA sequence was 98 % homologous to that reported by Clayton CL (688 out of 702 residues were identical). The derived amino acid sequences of the ureA were 99 % homologous to that reported by Clayton CL (232 out of 234 residues were identical). The nucleotide sequence and the predicted protein showed significant homology to ureA of H.pylori in the NCBI Entrez database.展开更多
Recently, much research has indicated that more and more cancers pose a threat to human life. Cancers are caused by oncogenes. Many human oncogenes have been found and most of them are located on chromosomes. The disc...Recently, much research has indicated that more and more cancers pose a threat to human life. Cancers are caused by oncogenes. Many human oncogenes have been found and most of them are located on chromosomes. The discovery of the oncogene plays a significant role in the treatment of cancer. The p53 tumor suppressor gene has received much attention because it frequently mutates or deletes in tumor cells of most people. Thus, the study of oncogenes is significant. In order to establish the Galois field (GF(7)), the indefinite gene is introduced as D and oncogene is introduced as O, and P. Taking the polynomial coefficients a0, a1, a2 ∈ GF(7) and the bijective function f: GF(7) → {D, A, C, O, G, T, P}, where f(0) = D, f(1) = A, f(2) = C, f(3) = O, f(4) = G, f(5) = T, and f(6)= P, the bijective → may be written as φ(a0 +a1x + a2x2). Based on the algebraic structure, we can not only analyse the DNA sequence of oncogenes, but also predict possible new cancers.展开更多
Many eukaryotic genes are members of multi-gene families due to gene duplications, which generate new copies that allow functional divergence. However, the relationship between
Autism is a heterogeneous neurodevelopmental and neuropsychiatric disorder with no precise etiology.Deficits in cognitive functions uncover at early stages and are known to have an environmental and genetic basis.Sinc...Autism is a heterogeneous neurodevelopmental and neuropsychiatric disorder with no precise etiology.Deficits in cognitive functions uncover at early stages and are known to have an environmental and genetic basis.Since autism is multifaceted and also linked with other comorbidities associated with various organs,there is a possibility that there may be a fundamental cellular process responsible for this.These reasons place mitochondria at the point of interest as it is involved in multiple cellular processes predominantly involving meta-bolism.Mitochondria encoded genes were taken into consideration lately because it is inher-ited maternally,has its own genome and also functions the time of embryo development.Various researches have linked mitochondrial mishaps like oxidative stress,ROS production and mt-DNA copy number variations to autism.Despite dramatic advances in autism research worldwide,the studies focusing on mitochondrial dysfunction in autism is rather minimal,especially in India.India,owing to its rich diversity,may be able to contribute significantly to autism research.It is vital to urge more studies in this domain as it may help to completely understand the basics of the condition apart from a genetic standpoint.This review focuses on the worldwide and Indian scenario of autism research;mitochondrial abnormalities in autism and possible therapeutic approaches to combat it.展开更多
文摘A predicted tau glutathione S-transferase(GST) subunit encoding gene,named GhGST,was isolated from Gossypium hirsutum with RACE method from SSH library based on Verticillium
基金funded by the National Key Research and Development Program of China(Nos.2019YFE0190900,2018YFD0900705).
文摘Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ascidians,including those expressed in the larval tail muscle,the adult body-wall muscle,and adult heart muscle.In this study,a novel striated non-tail muscle actin gene was identified from the RNA-seq data of Ciona savignyi embryos.Phylogenetic analysis,alignment of the N-terminal amino acid sequences and comparation of diagnostic residues provided evidence that it had high similarity with vertebrate cardiac and skeletal muscle actin.In situ hybridization and promoter-driven GFP reporter assay revealed that it was specifically expressed in the primordia of the oral and atrial siphon.We hereby defined it as siphon-specific muscle actin coding gene(Cs-SMA).A 201 bp(−1350 bp to−1150 bp)sequence containing T-box and Six1/2 binding motif within the upstream region of Cs-SMA confined the expression of GFP in the siphons of electroporated embryos.Six1/2 binding motif was experimentally confirmed to play indispensable role in controlling the siphon-specific expression of Cs-SMA.The tissue-specific expression of Cs-SMA in the siphon primordia indicated its potential crucial roles in Ciona embryogenesis and organogenesis.
文摘GPX-GI is a cytosolic tetrameric Se-dependent glutathione peroxidase, similar in properties to GPX-1. Unlike the almost ubiquitous GPX-1, GPX-GI is mainly expressed in the epithelium of gastrointestinal tract. GPX-GI contributes to at least fifty percent of GPX activity in rodent small intestmal epithelium. The total GPX activity consists of at least 70% of selenium-dependent GPX activity in this compartment.By analyzing a panel of mouse mterspecies DNA from the Jackson Laboratory's backcross resource,we mapped Gpx2 gene to mouse chromosome 12 between D12Mit4 and D12Mit5, near the Ccs1 locus which contains a colon cancer susceptibility gene. A pseudogene, Gpx2-ps is mapped to mouse chromosome 7.Comparison of Gpx2 gene expression in three pairs of C57BL/6Ha and ICR/Ha mice which are respectively resistant and sensitive to dimethylhydrazine-induced colon cancer, we found a higher Gpx2 mRNA level in C57BL/6Ha colon than ICR/Ha colon. Interestingly, a lower level of GPX activity is found in the resistant strain of mice. Because GPX-1 has three times higher specific activity than GPX GI, our data suggest that the decreased GPX activity may result from a higher level of Gpx2 gene expression in those cells co-express GPx1 gene
文摘High affinity phosphate transporterplays an important role in plantadapting to low phosphorus. Isolationof genes coding this kind of proteinhas attracted worldwide scholars toaccomplish. We aimed to isolate thegene and transfer it to target plants
文摘The gene encoding urease subunit A (ureA) of Helicobacter pylori (H.pylori) was cloned from H.pylori isolate by polymerase chain reaction (PCR). Sterile distilled water instead of DNA served as negative control. The nucleotide sequence of the amplified product was determined. Homologous analysis of the ureA against that reported by Clayton CL and the GenBank and SwissProt databases were performed with the BLAST program at the Genome Net through the Internet. 0.8 kb PCR product was amplified from all H.pylori clinical isolators. The nucleotide sequence of the ureA was determined. The nucleotide sequence of the ureA began with ATG as the initiation codon and terminated in TAA as stop codon. The coding regions had a 44 % G+C content. The DNA sequence was 98 % homologous to that reported by Clayton CL (688 out of 702 residues were identical). The derived amino acid sequences of the ureA were 99 % homologous to that reported by Clayton CL (232 out of 234 residues were identical). The nucleotide sequence and the predicted protein showed significant homology to ureA of H.pylori in the NCBI Entrez database.
基金Project supported in part by the Program for Innovative Research Team of Jiangnan University,China(Grant No.2008CX002)
文摘Recently, much research has indicated that more and more cancers pose a threat to human life. Cancers are caused by oncogenes. Many human oncogenes have been found and most of them are located on chromosomes. The discovery of the oncogene plays a significant role in the treatment of cancer. The p53 tumor suppressor gene has received much attention because it frequently mutates or deletes in tumor cells of most people. Thus, the study of oncogenes is significant. In order to establish the Galois field (GF(7)), the indefinite gene is introduced as D and oncogene is introduced as O, and P. Taking the polynomial coefficients a0, a1, a2 ∈ GF(7) and the bijective function f: GF(7) → {D, A, C, O, G, T, P}, where f(0) = D, f(1) = A, f(2) = C, f(3) = O, f(4) = G, f(5) = T, and f(6)= P, the bijective → may be written as φ(a0 +a1x + a2x2). Based on the algebraic structure, we can not only analyse the DNA sequence of oncogenes, but also predict possible new cancers.
文摘Many eukaryotic genes are members of multi-gene families due to gene duplications, which generate new copies that allow functional divergence. However, the relationship between
基金This work was supported by Science and Engineering Research Board(SERB)[grant number ECR/2016/001688].
文摘Autism is a heterogeneous neurodevelopmental and neuropsychiatric disorder with no precise etiology.Deficits in cognitive functions uncover at early stages and are known to have an environmental and genetic basis.Since autism is multifaceted and also linked with other comorbidities associated with various organs,there is a possibility that there may be a fundamental cellular process responsible for this.These reasons place mitochondria at the point of interest as it is involved in multiple cellular processes predominantly involving meta-bolism.Mitochondria encoded genes were taken into consideration lately because it is inher-ited maternally,has its own genome and also functions the time of embryo development.Various researches have linked mitochondrial mishaps like oxidative stress,ROS production and mt-DNA copy number variations to autism.Despite dramatic advances in autism research worldwide,the studies focusing on mitochondrial dysfunction in autism is rather minimal,especially in India.India,owing to its rich diversity,may be able to contribute significantly to autism research.It is vital to urge more studies in this domain as it may help to completely understand the basics of the condition apart from a genetic standpoint.This review focuses on the worldwide and Indian scenario of autism research;mitochondrial abnormalities in autism and possible therapeutic approaches to combat it.