Obejctive To study the lymphocytes, perforin protein and mRNA expression in endometrium of different phases in the menstrual cycle and to explore the regularity and role of the lymphocytes and perforin in the reprodu...Obejctive To study the lymphocytes, perforin protein and mRNA expression in endometrium of different phases in the menstrual cycle and to explore the regularity and role of the lymphocytes and perforin in the reproductive system Methods Immunohistochemical and in situ hybridization techniques were employed to demonstrate the population, phenotype and mediator (perforin) of lymphocytes in the proliferative and secretory endometrium Results In different phases of the endometrium, there were very few CD 3 + typical T lymphocytes, CD 4 + helper lymphocytes and CD 8 + suppressor/cytotoxic T lymphocytes, while CD 56 + lymphocyte (also named NK like cell) were abundant CD 56 + lymphocytes were absent in postmenopausal endometrium A double immunohistochemical labelling method demonstrated the co expression of perforin protein and CD 56 antigen of cells in different phases of the endometrium Quantitative analysis of CD 56 + lymphocytes and perforin positive cells increased in number from the proliferative to the secretory endometrium ( P <0 05) In situ hybridization analysis showed that perforin mRNA positive cells formed islands in proliferative endometrial stroma, but were scattered in secretory endometrium There was difference between perforin gene expression and protein expression in the proliferative endometrium Conclusions Lymphocytes and perforin expression in the endometrium at different phases of the menstrual cycle are specific and may play an important role in the reproductive system They may exert a positive influence on embryo implantation and be involved in endometrial stroma breakdown during menstruation展开更多
MicroRNAs （miRNAs） are a recently discovered class of non-coding RNAs that are expressed in many cell types, where they regulate the expression of complementary RNAs, thus modulating the stability and translation of...MicroRNAs （miRNAs） are a recently discovered class of non-coding RNAs that are expressed in many cell types, where they regulate the expression of complementary RNAs, thus modulating the stability and translation of mRNAs, miRNAs are predicted to regulate the expression of -50% of all protein coding genes in mammals. Therefore, they participate in virtually all cellular processes investigated so far. Altered miRNAs expressions are associated with both physiological （pregnancy） and pathological processes （cancer）. As the dynamic maternal-fetal interface plays a critical role in the maintenance of successful pregnancy, it is not surprising that the miRNAs that are unique to reproductive tissues are abundantly expressed. Research in this field has demonstrated the presence and dysregulation of a distinct set of pregnancy-associated miRNAs; however, most studies have centered on localizing various miRNAs in reproductive microdomains associated with normal or complicated pregnancies. Although several independent miRNA regulatory mechanisms associated with endometrial receptivity, immune cells, angiogenesis and placental development have been studied, miRNA-mediated regulation of pregnancy remains poorly understood. This review provides a summary of the current data on miRNA regulation as well as functional profiles of miRNAs that are found in the uterus, in immune cells associated with maternal tolerance to the fetus, and those involved in angiogenesis and placental development.展开更多
文摘Obejctive To study the lymphocytes, perforin protein and mRNA expression in endometrium of different phases in the menstrual cycle and to explore the regularity and role of the lymphocytes and perforin in the reproductive system Methods Immunohistochemical and in situ hybridization techniques were employed to demonstrate the population, phenotype and mediator (perforin) of lymphocytes in the proliferative and secretory endometrium Results In different phases of the endometrium, there were very few CD 3 + typical T lymphocytes, CD 4 + helper lymphocytes and CD 8 + suppressor/cytotoxic T lymphocytes, while CD 56 + lymphocyte (also named NK like cell) were abundant CD 56 + lymphocytes were absent in postmenopausal endometrium A double immunohistochemical labelling method demonstrated the co expression of perforin protein and CD 56 antigen of cells in different phases of the endometrium Quantitative analysis of CD 56 + lymphocytes and perforin positive cells increased in number from the proliferative to the secretory endometrium ( P <0 05) In situ hybridization analysis showed that perforin mRNA positive cells formed islands in proliferative endometrial stroma, but were scattered in secretory endometrium There was difference between perforin gene expression and protein expression in the proliferative endometrium Conclusions Lymphocytes and perforin expression in the endometrium at different phases of the menstrual cycle are specific and may play an important role in the reproductive system They may exert a positive influence on embryo implantation and be involved in endometrial stroma breakdown during menstruation
文摘MicroRNAs （miRNAs） are a recently discovered class of non-coding RNAs that are expressed in many cell types, where they regulate the expression of complementary RNAs, thus modulating the stability and translation of mRNAs, miRNAs are predicted to regulate the expression of -50% of all protein coding genes in mammals. Therefore, they participate in virtually all cellular processes investigated so far. Altered miRNAs expressions are associated with both physiological （pregnancy） and pathological processes （cancer）. As the dynamic maternal-fetal interface plays a critical role in the maintenance of successful pregnancy, it is not surprising that the miRNAs that are unique to reproductive tissues are abundantly expressed. Research in this field has demonstrated the presence and dysregulation of a distinct set of pregnancy-associated miRNAs; however, most studies have centered on localizing various miRNAs in reproductive microdomains associated with normal or complicated pregnancies. Although several independent miRNA regulatory mechanisms associated with endometrial receptivity, immune cells, angiogenesis and placental development have been studied, miRNA-mediated regulation of pregnancy remains poorly understood. This review provides a summary of the current data on miRNA regulation as well as functional profiles of miRNAs that are found in the uterus, in immune cells associated with maternal tolerance to the fetus, and those involved in angiogenesis and placental development.
