To characterize the Fe(III)-reducing bacteria,enrichment cultures were initiated by inoculating deep-sea sediment from the South China Sea(SCS)into the media with hydrous ferric oxide(HFO)as the sole electron acceptor...To characterize the Fe(III)-reducing bacteria,enrichment cultures were initiated by inoculating deep-sea sediment from the South China Sea(SCS)into the media with hydrous ferric oxide(HFO)as the sole electron acceptor.As indicated by Meta 16S rDNA Amplicon Sequencing,the microorganisms related to Fe(III)-reduction in the enrichment cultures were mainly Shewanella and Enterobacter.A new facultative Fe(III)-reducing bacterium was obtained and identified as Enterobacter sp.Nan-1 based on its 16S rRNA gene sequence and physiological characterizations.Enterobacter sp.Nan-1 was not only a mesophilic bacterium capable of reducing HFO with a wide range of salinity(4,34,40,50 and 60 g L−1)efficiently,but also a piezotolerant bacterium that can proceed Fe(III)-reduction sustainedly at hydrostatic pressures between 0.1 and 50 MPa using glucose and pyruvate as carbon source.Furthermore,the geochemical characteristics of deep-sea sediment indicated that the microbial metabolism and iron reduction both remain active in the well-developed Fe(III)-reducing zone where the strain Nan-1 was obtained.To our knowledge,Enterobacter sp.Nan-1 could serve as a new applicative Fe(III)-reducing bacterium for future investigation on the iron biogeochemical cycle and diagenetic process of organic matter in the deep-sea environment.展开更多
A novel alkalizing strain Enterobacter sp.LYX-2 that could resist 400 mg/L Cd was isolated from Cd-contaminated soil,which immobilized 96.05%Cd^(2+)from medium.Cd distribution analysis demonstrated that more than half...A novel alkalizing strain Enterobacter sp.LYX-2 that could resist 400 mg/L Cd was isolated from Cd-contaminated soil,which immobilized 96.05%Cd^(2+)from medium.Cd distribution analysis demonstrated that more than half of the Cd^(2+)was converted into extracellular precipitated Cd through mobilization of the alkali-producing mechanism by the strain LYX-2,achieving the high immobilization efficiency of Cd^(2+).Biosorption experiments revealed that strain LYX-2 had superior biosorption capacity of 48.28 mg/g for Cd.Pot experiments with Brassica rapa L.were performed with and without strain LYX-2.Compared to control,15.92%bioavailable Cd was converted to non-bioavailable Cd and Cd content in aboveground vegetables was decreased by 37.10%with addition of strain LYX-2.Available Cd was mainly immobilized through extracellular precipitation,cell-surface biosorption and intracellular accumulation of strain LYX-2,which was investigated through Cd distribution,Scanning Electron Microscope and Energy-Dispersive X-ray Spectroscopy(SEM-EDS),Fourier Transform Infrared Spectroscopy(FT-IR),X-ray Photoelectron Spectroscopy(XPS)and Transmission Electron Microscopy(TEM)analysis.In addition,the application of strain LYX-2 significantly promoted the growth of vegetables about 2.4-fold.Above results indicated that highly Cd-resistant alkalizing strain LYX-2,as a novel microbial passivator,had excellent ability and reuse value to achieve the remediation of Cd-contaminated soil coupled with safe production of vegetables simultaneously.展开更多
基金the financial support by the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB06020000)the Zhejiang Geological Prospecting Bureau Science Projects(No.201713)the Geological Fund of Zhejiang Province(No.20150012).
文摘To characterize the Fe(III)-reducing bacteria,enrichment cultures were initiated by inoculating deep-sea sediment from the South China Sea(SCS)into the media with hydrous ferric oxide(HFO)as the sole electron acceptor.As indicated by Meta 16S rDNA Amplicon Sequencing,the microorganisms related to Fe(III)-reduction in the enrichment cultures were mainly Shewanella and Enterobacter.A new facultative Fe(III)-reducing bacterium was obtained and identified as Enterobacter sp.Nan-1 based on its 16S rRNA gene sequence and physiological characterizations.Enterobacter sp.Nan-1 was not only a mesophilic bacterium capable of reducing HFO with a wide range of salinity(4,34,40,50 and 60 g L−1)efficiently,but also a piezotolerant bacterium that can proceed Fe(III)-reduction sustainedly at hydrostatic pressures between 0.1 and 50 MPa using glucose and pyruvate as carbon source.Furthermore,the geochemical characteristics of deep-sea sediment indicated that the microbial metabolism and iron reduction both remain active in the well-developed Fe(III)-reducing zone where the strain Nan-1 was obtained.To our knowledge,Enterobacter sp.Nan-1 could serve as a new applicative Fe(III)-reducing bacterium for future investigation on the iron biogeochemical cycle and diagenetic process of organic matter in the deep-sea environment.
基金supported by the National Key Research and Development Program of China (No.2018YFC1800403)the National Natural Science Foundation of China (No.41571226)the Scientific Research Fund of Zhejiang University (No.XY2021020)。
文摘A novel alkalizing strain Enterobacter sp.LYX-2 that could resist 400 mg/L Cd was isolated from Cd-contaminated soil,which immobilized 96.05%Cd^(2+)from medium.Cd distribution analysis demonstrated that more than half of the Cd^(2+)was converted into extracellular precipitated Cd through mobilization of the alkali-producing mechanism by the strain LYX-2,achieving the high immobilization efficiency of Cd^(2+).Biosorption experiments revealed that strain LYX-2 had superior biosorption capacity of 48.28 mg/g for Cd.Pot experiments with Brassica rapa L.were performed with and without strain LYX-2.Compared to control,15.92%bioavailable Cd was converted to non-bioavailable Cd and Cd content in aboveground vegetables was decreased by 37.10%with addition of strain LYX-2.Available Cd was mainly immobilized through extracellular precipitation,cell-surface biosorption and intracellular accumulation of strain LYX-2,which was investigated through Cd distribution,Scanning Electron Microscope and Energy-Dispersive X-ray Spectroscopy(SEM-EDS),Fourier Transform Infrared Spectroscopy(FT-IR),X-ray Photoelectron Spectroscopy(XPS)and Transmission Electron Microscopy(TEM)analysis.In addition,the application of strain LYX-2 significantly promoted the growth of vegetables about 2.4-fold.Above results indicated that highly Cd-resistant alkalizing strain LYX-2,as a novel microbial passivator,had excellent ability and reuse value to achieve the remediation of Cd-contaminated soil coupled with safe production of vegetables simultaneously.
