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A HPLC method for the determination of entrapment efficiency of a new 5-FPE liposome formulation
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作者 向蓉 陈世武 +2 位作者 张辅民 倪京满 田瑄 《Journal of Chinese Pharmaceutical Sciences》 CAS 2009年第2期186-189,共4页
A simple HPLC method was established for the determination of entrapment efficiency of a new 5-FPE liposome formulation. Chromatographic separation was performed on a Kromasil 100 A C18 column (350 mm×4.6 mm, 5 ... A simple HPLC method was established for the determination of entrapment efficiency of a new 5-FPE liposome formulation. Chromatographic separation was performed on a Kromasil 100 A C18 column (350 mm×4.6 mm, 5 μm). The mobile phase was consisted of methanol-water (58:42, v/v). The flow rate of mobile phase was set at 0.8 mL/min. The UV detection wavelength was 271 nm, and the column temperature was 30 ℃. The linear range of 5-FPE was from 0.8-12.8 μg/mL, r = 0.9999. The RSD of intm-day and inter-day precision were less than 2.97%. The average recovery was from 96.8%-104.6% with RSD less than 2.24%. The method was simple, rapid, accurate, and sensitive. It is suitable for the determination of entrapment efficiency of the 5-FPE liposome formulation. 展开更多
关键词 Podophyllotoxin HPLC entrapment efficiency LIPOSOME
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Pharmaceutical Characteristics of Daunorubicin Stealth Liposomes
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作者 张华 齐宪荣 张强 《Journal of Chinese Pharmaceutical Sciences》 CAS 2001年第4期200-202,共3页
This report studied on pharmaceutical characteristics of the stealth liposome containing dau-norubicin (DNR). The shape, size, entrapment efficiency and stability of the daunorubicin stealth liposomes (DNRSL) were exa... This report studied on pharmaceutical characteristics of the stealth liposome containing dau-norubicin (DNR). The shape, size, entrapment efficiency and stability of the daunorubicin stealth liposomes (DNRSL) were examined. Visible spectrophotometry and the HPLC method were established for determination of the DNR in the DNRSL. The release of DNR from DNRSL in HBS (pH 7.5) and rat serum at 37 oC were examined. The results showed that the DNRSL had high entrapment efficiency (>85%), small size and slow release. 展开更多
关键词 Stealth liposomes DAUNORUBICIN entrapment efficiency Drug release in vitro
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Immunological Effect of Subunit Influenza Vaccine Entrapped by Liposomes
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作者 SHUI-HUA ZHANG JIA-XU LIANG +3 位作者 SHU-YAN DAI XIAO-LIN QIU YAN-RONG YI YUN PAN 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2009年第5期388-393,共6页
Objective To elevate the immunological effect of subunit influenza vaccine in infants and aged people (over 60) using liposomal adjuvant in the context of its relatively low immunity and to investigate the relation ... Objective To elevate the immunological effect of subunit influenza vaccine in infants and aged people (over 60) using liposomal adjuvant in the context of its relatively low immunity and to investigate the relation between vaccine antigens and liposomal characteristics. Methods Several formulations of liposomal subunit influenza vaccine were prepared. Their relevant characteristics were investigated to optimize the preparation method. Antisera obtained from immunizinged mice were used to evaluate the antibody titers of various samples by HI and EL1SA. Results Liposomal trivalent influenza vaccine prepared by film evaporation in combinedation with freeze-drying significantly increased its immunological effect in SPF Balb/e mice. Liposomal vaccine stimulated the antibody titer of H3N2, H1N1, and B much stronger than conventional influenza vaccine. As a result, liposomal vaccine (mean size: 4.5-5.5 pm, entrapment efficiency: 30%-40%) significantly increased the immunological effect of subunit influenza vaccine. Conclusion The immune effect of liposomal vaccine depends on different antigens, and enhanced immunity is not positively correlated with the mean size of liposome or its entrapped efficiency. 展开更多
关键词 LIPOSOME Subunit influenza vaccine Size distribution Mean size Entrapped efficiency
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