Deformation mechanism and roof pre-splitting control technology of gob-side entry in thick hard main roof full-mechanized longwall caving panel

This paper explores the deformation mechanism and control technology of roof pre-splitting for gob-side entries in hard roof full-mechanized longwall caving panel(LTCC).The investigation utilizes a comprehensive approach that integrates field monitoring,theoretical analysis,and numerical simulation.Theoretical analysis has illuminated the influence of the length of the lateral cantilever beam of the main roof(LCBM)above the roadway on the stability of the gob-side entry behind the panel.Numerical simulations have further revealed that the longer LCBM results in heightened vertical stress within the coal pillar,developed cracks around the roadway,and more pronounced damage to the roadway.Moreover,numerical simulations also demonstrate the potential of roof pre-splitting technology in optimizing the fracture position of the hard roof.This technology significantly reduces the length of the LCBM,thereby alleviating stress concentration in the coal pillars and integrated coal rib while minimizing the destruction of the gob-side entry.Therefore,this manuscript first proposes the use of roof pre-splitting technology to control roadway deformation,and automatically retain the entry within a hard roof LTCC panel.Field implementation has demonstrated that the proposed automatically retained entry by roof pre-splitting technology effectively reduces gob-side entry deformation and achieves automatically retained entry.

Ground response and failure mechanism of gob-side entry by roof cutting with hard main roof

This study is the result of long-term efforts of the authors’team to assess ground response of gob-side entry by roof cutting(GSERC)with hard main roof,aiming at scientific control for GSERC deformation.A comprehensive field measurement program was conducted to determine entry deformation,roof fracture zone,and anchor bolt(cable)loading.The results indicate that GSERC deformation presents asymmetric characteristics.The maximum convergence near roof cutting side is 458 mm during the primary use process and 1120 mm during the secondary reuse process.The entry deformation is closely associated with the primary development stage,primary use stage,and secondary reuse stage.The key block movement of roof cutting structure,a complex stress environment,and a mismatch in the supporting design scheme are the failure mechanism of GSERC.A controlling ideology for mining states,including regional and stage divisions,was proposed.Both dynamic and permanent support schemes have been implemented in the field.Engineering practice results indicate that the new support scheme can efficiently ensure long-term entry safety and could be a reliable approach for other engineering practices.

Polypyrimidine Tract-Binding Protein Enhances Zika Virus Translation by Binding to the 5'UTR of Internal Ribosomal Entry Site

Objectives To identify the 5'untranslated region of Zika virus(ZIKV 5'UTR)RNA-binding proteins and to investigate the impact of the binding protein on the activity of internal ribosomal entry site(IRES)located in ZIKV 5'UTR and virus production.Methods Interacting proteins in U251 cells were captured using tRSA-tagged ZIKV 5'UTR RNA and tRSA-ZIKV 5'UTR RNA-binding proteins were visualized by SDS-PAGE silver staining,Subsequently,liquid chromatographytandem mass spectrometry(LC-MS/MS),bioinformatics analysis,and Western blot were used to identify the candidate proteins binding to ZIKV 5'UTR.Dicistronic expression assay and plaque forming assay were performed to analyze the effect of the binding protein on ZIKV IRES activity and ZIKV production,respecitvely.Results tRSA RNA pull-down assay,LC-MS/MS,and Western blot analysis showed that polypyrimidine tractbinding protein(PTB)bound to the ZIKV 5'UTR.Furthermore,dual luciferase reporter assay revealed that overexpression of PTB significantly enhanced the IRES activity of ZIKV(t=10.220,P<0.001),while PTB knockdown had the opposite effect(t=4.897,P<0.01).Additionally,virus plaque forming assay demonstrated that up-regulation of PTB expression significantly enhanced viral titer(t=6.400,P<0.01),whereas reducing PTB expression level weakened virus infectivity(t=5.055,P<0.01).Conclusion PTB positively interacts with the ZIKV 5'UTR and enhances IRES activity and virus production.

Roles of host proteases in the entry of SARS-CoV-2

The spike protein(S)of SARS-CoV-2 is responsible for viral attachment and entry,thus a major factor for host suscep-tibility,tissue tropism,virulence and pathogenicity.The S is divided with S1 and S2 region,and the S1 contains the receptor-binding domain(RBD),while the S2 contains the hydrophobic fusion domain for the entry into the host cell.Numerous host proteases have been implicated in the activation of SARS-CoV-2 S through various c leavage sites.In this article,we review host proteases including furin,trypsin,transmembrane protease serine 2(TMPRSS2)and cathepsins in the activation of SARS-CoV-2 S.Many betacoronaviruses including SARS-CoV-2 have polybasic residues at the S1/S2 site which is subjected to the cleavage by furin.The S1/S2 cleavage facilitates more assessable RBD to the receptor ACE2,and the binding triggers further conformational changes and exposure of the S2'site to proteases such as type Il transmembrane serine proteases(TTPRs)including TMPRSS2.In the presence of TMPRSS2 on the target cells,SARS-CoV-2 can utilize a direct entry route by fusion of the viral envelope to the cellular membrane.In the absence of TMPRSS2,SARS-CoV-2 enter target cells via endosomes where multiple cathepsins cleave the S for the successful entry.Additional host proteases involved in the cleavage of the S were discussed.This article also includes roles of 3C-like protease inhibitors which have inhibitory activity against cathepsin L in the entry of SARS-CoV-2,and discussed the dual roles of such inhibitors in virus replication.

Application of Thermodynamic Database to Corrosion of ZrO_(2) Containing Submerged Entry Nozzle in Steel Continuous Casting Process

The CALPHAD thermodynamic databases are very useful to analyze the complex chemical reactions happening in high temperature material process.The FactSage thermodynamic database can be used to calculate complex phase diagrams and equilibrium phases involving refractories in industrial process.In this study,the FactSage thermodynamic database relevant to ZrO_(2)-based refractories was reviewed and the application of the database to understanding the corrosion of continuous casting nozzle refractories in steelmaking was presented.

Ca^(2+)-induced myelin pathology precedes axonal spheroid formation and is mediated in part by store-operated Ca^(2+)entry after spinal cord injury

The formation of axonal spheroid is a common feature following spinal cord injury.To further understand the source of Ca^(2+)that mediates axonal spheroid formation,we used our previously characterized ex vivo mouse spinal cord model that allows precise perturbation of extracellular Ca^(2+).We performed twophoton excitation imaging of spinal cords isolated from Thy1YFP+transgenic mice and applied the lipophilic dye,Nile red,to record dynamic changes in dorsal column axons and their myelin sheaths respectively.We selectively released Ca^(2+)from internal stores using the Ca^(2+)ionophore ionomycin in the presence or absence of external Ca^(2+).We reported that ionomycin dose-dependently induces pathological changes in myelin and pronounced axonal spheroid formation in the presence of normal 2 m M Ca^(2+)artificial cerebrospinal fluid.In contrast,removal of external Ca^(2+)significantly decreased ionomycin-induced myelin and axonal spheroid formation at 2 hours but not at 1 hour after treatment.Using mice that express a neuron-specific Ca^(2+)indicator in spinal cord axons,we confirmed that ionomycin induced significant increases in intra-axonal Ca^(2+),but not in the absence of external Ca^(2+).Periaxonal swelling and the resultant disruption in the axo-myelinic interface often precedes and is negatively correlated with axonal spheroid formation.Pretreatment with YM58483(500 n M),a well-established blocker of store-operated Ca^(2+)entry,significantly decreased myelin injury and axonal spheroid formation.Collectively,these data reveal that ionomycin-induced depletion of internal Ca^(2+)stores and subsequent external Ca^(2+)entry through store-operated Ca^(2+)entry contributes to pathological changes in myelin and axonal spheroid formation,providing new targets to protect central myelinated fibers.

Experimental observation on water entry of a sphere in regular wave

This paper presents a novel experiment to observe the whole water entry process of a free-falling sphere into a regular wave.A time-accurate synchronizing system modulates the moment elaborately to ensure the sphere impacting onto the water surface at the desirable wave phase.Four high-speed cameras focus locally to measure the high-precision size of the cavity evolution.Meanwhile,the aggregated field view of the camera array covers both the splash above the free surface and the entire cavity in the wave.The detailed methodologies are described and verified for the hardware set-up and the image post-processing.The theoretical maximum deviation is 1.7%on the space scale.The integral morphology of the cavity is captured precisely in the coordinate system during the sphere penetrates through the water at four representative wave phases and the still water.The result shows that the horizontal velocity of the fluid particle in the wave impels the cavity and changes the shape distinctly.Notably,the wave motion causes the cavity to pinch offearlier at the wave trough phase and later at the wave crest phase than in the still water.The wave motion influences the falling process of the sphere slightly in the present parameters.

Eye-shaped keyboard for dual-hand text entry in virtual reality

We propose an eye-shaped keyboard for high-speed text entry in virtual reality (VR), having the shape of dual eyes with characters arranged along the curved eyelids, which ensures low density and short spacing of the keys. The eye-shaped keyboard references the QWERTY key sequence, allowing the users to benefit from their experience using the QWERTY keyboard. The user interacts with an eye-shaped keyboard using rays controlled with both the hands. A character can be entered in one step by moving the rays from the inner eye regions to regions of the characters. A high-speed auto-complete system was designed for the eye-shaped keyboard. We conducted a pilot study to determine the optimal parameters, and a user study to compare our eye-shaped keyboard with the QWERTY and circular keyboards. For beginners, the eye-shaped keyboard performed significantly more efficiently and accurately with less task load and hand movement than the circular keyboard. Compared with the QWERTY keyboard, the eye-shaped keyboard is more accurate and significantly reduces hand translation while maintaining similar efficiency. Finally, to evaluate the potential of eye-shaped keyboards, we conducted another user study. In this study, the participants were asked to type continuously for three days using the proposed eye-shaped keyboard, with two sessions per day. In each session, participants were asked to type for 20min, and then their typing performance was tested. The eye-shaped keyboard was proven to be efficient and promising, with an average speed of 19.89 words per minute (WPM) and mean uncorrected error rate of 1.939%. The maximum speed reached 24.97 WPM after six sessions and continued to increase.

An Innovative Non-Pillar Coal-Mining Technology with Automatically Formed Entry: A Case Study

A non-pillar coal-mining technology with an automatically formed entry is proposed,which reduces the waste of coal resources and the underground entry drivage workload.Three key techniques in this technology cooperate to achieve automatic formation and retaining of the gob-side entry,and to realize nonpillar mining.Constant-resistance large deformation(CRLD)support ensures the stability of the entry roof;directional presplitting blasting(DPB)separates the entry roof and the gob roof;and a blockinggangue support system(BGSS)integrates the caved rock material as an effective entry rib.An industrial test was conducted to verify the engineering effects of these key techniques.The field application results showed that the retained entry was under the pressure-relief zone due to the broken-expansion nature of the caved rock mass within the DPB height.After going through a provisional dynamic pressure-bearing zone,the retained entry entered the stability zone.The final stable entry meets the requirements of safety and production.The research results demonstrate the good engineering applicability of this technology.By taking the framework of the technology design principles into consideration and adjusting the measures according to different site conditions,it is expected that the proposed non-pillar coal-mining technology can be popularized on a large scale.

Patterns and security technologies for co-extraction of coal and gas in deep mines without entry pillars

Retaining gob-side entryways and the stability of gas drainage boreholes are two essential techniques in the co-extraction of coal and gas without entry pillars （CECGWEP）. However, retained entryways located in deep coal mines are hard to maintain, especially for constructing boreholes in confined spaces, owing to major deformations. Consequently, it is difficult to drill boreholes and maintain their stability, which therefore cannot guarantee the effectiveness of gas drainage. This paper presents three measures for conducting CECGWEP in deep mines on the basis of effective space in retained entryways for gas drainage, They are combinations of retaining roadways and face-lagging inclined boreholes, retaining roadways and face-advancing inclined boreholes, and retaining roadways and high return airway inclined boreholes. Several essential techniques are suggested to improve the maintenance of retained entryways and the stabilization of boreholes. For the particular cases considered in this study, two field trials have verified the latter two measures from the results obtained from the faces 1111（1） and 11112（1） in the Zhuji Mine. The results indicate that these models can effectively solve the problems in deep mines. The maximum gas drainage flow for a single hole can reach 8.1 m^3/min and the effective drainage distance can be extended up to 150 m or more.

New insights into store-independent Ca^(2+) entry: secretory pathway calcium ATPase 2 in normal physiology and cancer

Recent studies in secretory pathway calcium ATPases （SPCA） revealed novel functions of SPCA2 in interacting with store-operated Ca2＋ channel Oral I and inducing Ca2＋ influx at the cell surface. Importantly, SPCA2-mediated Ca2＋ signaling is uncoupled from its conventional role of Ca2＋-ATPase and independent of store-operated Ca2＋ signaling pathway. SPCA2-induced store-independent Ca2＋ entry （SICE） plays essential roles in many important physiological processes, while unbalanced SICE leads to enhanced cell proliferation and tumorigenesis. Finally, we have summarized the clinical implication of SICE in oral cancer prognosis and treatment. Inhibition of SICE may be a new target for the development of cancer therapeutics.

Some Aerothermodynamic Aspects of ESA Entry Probes

Two different entry vehicles are presented here： the Inflatable Reentry and Descent Demonstrator （IRDT）, and Huygens. Both missions involve （re）entries at conditions close to orbital, and have been performed in 2005. Specific aspects of the design and the mission of IRDT are briefly outlined. The preliminary results of the recent flight of IRDT and the methodology followed at ESTEC for the assessment of radiative fluxes for Huygens are summarised.

Virus-host Interactions during Hepatitis C Virus Entry-Implications for Pathogenesis and Novel Treatment Approaches

Hepatitis C virus (HCV) is a member of the Flaviviridae family and causes acute and chronic hepatitis. Chronic HCV infection may result in severe liver damage including liver cirrhosis and hepatocellular carcinoma. The liver is the primary target organ of HCV, and the hepatocyte is its primary target cell. Attachment of the virus to the cell surface followed by viral entry is the first step in a cascade of interactions between the virus and the target cell that is required for successful entry into the cell and initiation of infection. This step is an important determinant of tissue tropism and pathogenesis; it thus represents a major target for antiviral host cell responses, such as antibody-mediated virus neutralization. Following the development of novel cell culture models for HCV infection our understanding of the HCV entry process and mechanisms of virus neutralization has been markedly advanced. In this review we summarize recent developments in the molecular biology of viral entry and its impact on pathogenesis of HCV infection, development of novel preventive and therapeutic antiviral strategies.

Comparison and Analysis on Animal and Plant Quarantine of Entry Mails among China, Australia and New Zealand

With the significant increase of the number of entry mails in China, mail inspection has become more and more challenge. Australia and New Zealand are the two countries recognized as the ones with the most stringent inspection and quarantine measures in the world. The mail inspection work in China, Australia and New Zealand was compared on the aspects of mail quarantine system, inspection, quarantine treatment and punishment, based on which something worth learning and referring could be found and some suggestions were put forward, with the aim to provide theoretical basis for the animal and plant quarantine of entry mails.

Variation in the timing of river entry of Atlantic salmon (Salmo salar L.) in the Baltic

The timing of river entry in the Atlantic salmon is known to depend on genetic, demographic and environmental factors, but little is known about the relative magnitude of among population and among year variation and covariation in this respect in natural state Atlantic salmon rives. To investigate this, variability in the timing of river entry in three historical Finnish Atlantic salmon populations were analyzed using salmon trap data collected during 1870- 1902. The analyses reveled that 1 ） the timing of river entry differed substantially and consistently among the rivers, and that 2） variation among the rivers was much larger than variation among years. Annual variations were not explained by regional environmental conditions, whereas in one river the timing of the local flood peak was a significant predictor of the timing of river entry. Differences in the timing of salmon entry to geographically closely situated rivers suggests that a regionally fixed opening date for coastal fisheries might not be the best management strategy as it may lead to uneven exploitation of salmon populations from different rivers [ Current Zoology 55 （5） ： 342 - 349, 2009] .

Rock burst mechanism analysis in an advanced segment of gob-side entry under different dip angles of the seam and prevention technology

In order to investigate the frequent occurrences of rock burst in gob-side entry during the mining process of the mining zone No. 7, the mechanical model of main roof of fully-mechanized caving mining before breaking was established by the Winkler foundation beam theory, and the stress evolution law of surrounding rock with different dip angles of the seam during the mining process was analyzed by using FLAC3 D. The results show that: with the dip angle changing from 45° to 0°, the solid-coal side of gobside entry begins to form an L-shaped stress concentration zone at a dip angle of 30°, and the stress concentration degree goes to higher and higher levels. However, the stress concentration degree of the coalpillar side goes to lower and lower levels; the influence range and peak stress of the abutment at the lateral strata of adjacent gob increase with dip angle decreasing and reach a maximum value at a dip angle of 0°, but the tailgate is not affected; the abutment pressure superposition of two adjacent gobs leads to stress concentration further enhancing in both sides of gob-side entry. With the influence of strong mining disturbance, rock burst is easily induced by dynamic and static combined load in the advanced segment of gob-side entry. To achieve stability control similar to that in the roadway, the key control strategy is to reinforce surrounding rock and unload both sides. Accordingly, the large-diameter drilling and high-pressure water injection combined unloading and reinforced support cooperative control technology was proposed and applied in field test. The results of Electromagnetic Emission(EME) and field observation showed that unloading and surrounding rock control effect was obvious.

Clogging behavior of submerged entry nozzles for Ti-bearing IF steel

The nozzle clogging behavior of Ti-bearing IF steel was studied by metallographic analysis,scanning electron microscopy（SEM）, energy dispersive spectroscopy（EDS）,and X-ray diffraction（XRD）.According to the experimental results,nozzle clogging primarily appears three layers.There are a lot of large-sized iron particles in the inner layer and mainly slag phase in the middle and outer layers.The principal clog constituents of the inner layer are loose alumina cluster inclusions and granular shaped alumina inclusions,containing iron particles. The clog constituents of the middle layer are mainly dendrite alumina inclusions.The primary phases existing in nozzle clogging are FeO·TiO2 and FeO·Al2O3 besidesα-Al2O3 and a-Fe.The FeO·TiO2 phases among the deposits adhere the deposits together firmly enough to lead to the inferior castability of Ti-bearing ultra low carbon steel compared with that of Ti-free low carbon Al-killed steel.

UNDERWATER ACOUSTICS AND CAVITATING FLOW OF WATER ENTRY

The fluid mechanics of water entry is studied through investigating the underwater acoustics and the supercavitation.Underwater acoustic signals in water entry are extensively measured at about 30 different positions by using a PVDF needle hydrophone.From the measurements we obtain (1)the primary shock wave caused by the impact of the blunt body on free surface;(2)the vapor pressure inside the cavity;(3)the secondary shock wave caused by pulling away of the cavity from free surface;and so on.The supercavitation induced by the blunt body is observed by using a digital high-speed video camera as well as the single shot photography.The periodic and 3 dimensional motion of the supercavitation is revealed.The experiment is carried out at room temperature.

Superposed disturbance mechanism of sequential overlying strata collapse for gob-side entry retaining and corresponding control strategies

Gob-area roof rupture movement is a key disturbance factor for gob-side entry retaining.The characteristics of gob-area sequential roof collapse of overlying strata and superposed disturbance mechanism for gob-side entry retaining are obtained via physical simulation and theoretical analysis,in which the scope of disturbed strata is enlarged from main roof to fracture zone.The experiment reveals that as a working face advances,roof strata sequentially collapse from bottom to top and produce multiple disturbances to gob-side entry retaining.Key strata among the overlying strata control each collapse.Main roof subsidence is divided into three stages:flexure subsidence prior to rupture,rotational subsidence during rupture and compressive subsidence after rupture.The amounts of deformation evident in each of the three stages are 15%,55%and 30%,respectively.After the master stratum collapses,main roof subsidence approaches its maximum value.The final span of the key stratum determines the moment and cycling of gob-side entry retaining disturbances.Main roof subsidence influences the load on the filling wall.The sequential roof collapse of overlying strata results in fluctuations in the gob-side entry retaining deformation.Calculation formulae for the final span of the key stratum and the filling wall load are obtained via theoretical analysis.A control method for the stability of the gob-side entry retaining’s surrounding rock is proposed,which includes 3 measures:a“dual-layer”proactive anchorage support,roadside filling with dynamic strength matching and auxiliary support during disturbance.Finally,the gob-side entry retaining of the Xiaoqing mine E1403 working face is presented as an engineering case capable of verifying the validity of the research conclusions.

Surrounding rock control of gob-side entry driving with narrow coal pillar and roadway side sealing technology in Yangliu Coal Mine

Gob-side entry driving can increase coal recovery ratio, and it is implied in many coal mines. Based on geological condition of 10416 working face tailentry in Yangliu Coal Mine, the surrounding rock deformation characteristics of gob-side entry driving with narrow coal pillar is analysed, reasonable size of coal pillar and reasonable roadway excavation time after mining are achieved. Surrounding rock control technology and effective roadway side sealing technology are proposed and are taken into field practice. The results showed that a safer and more efficient mining of working face can be achieved. In addition, results of this paper also have important theoretical significance and valuable reference for surrounding rock control technology of gob-side entry driving with narrow coal pillar under special geological condition.