A novel mitigator of enzymatic browning--hawthorn leaf extract and its application in the preservation of fresh-cut potatoes

Objectives:The purpose of this study was to evaluate the antibrowning functions of hawthorn leaf extract on fresh-cut potato and its possible mechanism.Materials and Methods:Fresh-cut potatoes were treated with different concentrations(0.01%,0.05%,and 0.1%)of hawthorn leaf extract and preserved at 4 C for 8 days.The appears nee and colour of potato slices were evaluated,along with the content of the phenol,malondialdehyde(MDA),and hydrogen peroxide(H2O2)during cold storage.Meanwhile,the activities of polyphenol oxidase(PPO),peroxidase(POD),phenylalanine ammonia-lyase(PAL),lipoxygenase(LOX),catalase(CAT),superoxide dismutase(SOD),and the antioxidant capacity were determ in ed.Furthermore,the composition of hawthorn leaf extract was analyzed by high-performance liquid chromatographytandem mass spectrometry(HPLC-MS/MS).Results:The addition of hawthorn leaf extract effectively delayed the browning process.It not only enhanced the CAT activity and antioxidant capacity but also reduced the LOX activity and accumulation of MDA and H_(2)O_(2).Meanwhile,the activities of PPO,POD,and PAL as well as the content of phe nol were con trolled.Additionally,25 phe nols,34 flavonoids,and 5 proa nthocya nidins were identified through high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS),ideluding caffeic acid,quercetin and catechol.Conelusion:Hawthorn leaf extract significantly alleviated the browning of fresh-cut potato.It could serve as a n atural an tibrowning alter native by stabilizing the membra ne and modulating reactive oxygen species and redox reactions.

Widely Untargeted Metabolome Profiling Provides Insight into Browning and Nutritional Quality Changes in Short-Term Stored Fresh-Cut Potato(Solanum tuberosum L.)Shreds

Potato(Solanum tuberosum L.)is susceptible to enzymatic browning after fresh processing,resulting in color change and potential alteration in the nutritional quality.In this study,a popular potato cultivar,Feiwuruita,was used to profile the metabolites involved in color and nutritional quality changes in fresh shreds stored at 0 and 4 h at 25°C(designated CK and CK4H,respectively).The shreds turned brown within 4 h of storage.In all,723 metabolites consisting 12 classes of compounds were detected in the samples,largely lipids,phenolic acids,alkaloids,amino acids and derivatives,flavonoids,organic acids,nucleotides and derivatives.Of these,163 metabolites accumulated differentially between CK and CK4H shreds.Polyphenolic compounds(phenolic acids and flavonoids)mostly increased in the shreds after 4 h storage.Conversely,the short-term storage drastically reduced lipid compounds(25 LysoPC and 19 LysoPE),while essential alkaloids and terpenoid compounds that are beneficial to human health increased in accumulation.The findings present global metabolome and nutritional composition changes in short-term stored shreds of Feiwuruita.This study provides important foundation for future studies on browning prevention/reduction and for better utilization of Feiwuruita.

香蕉加工过程酶促褐变控制的研究

研究了香蕉果实不同部位的褐变情况以及去皮、切片、打浆、钝酶工艺中的褐变,通过褐变抑制试验,筛选了几种护色剂。结果表明,香蕉的果皮、果肉上的丝络、果心部位均比果肉易于褐变。去皮、切片、打浆处理对香蕉酶促褐变的影响程度由大到小为:打浆>切片>去皮,打浆时间越长,褐变程度越高。微波法钝酶比热水法、蒸汽法护色效果好,且能保持香蕉的风味。柠檬酸、异抗坏血酸、4-乙基间苯二酚、植酸分别对香蕉浆液均有护色作用,其最佳配比为:柠檬酸0.32%、异抗坏血酸0.06%、4-乙基间苯二酚0.12%、植酸0.02%。