Because of its low sensitivity to radiations, therapeutic ratio of neuroglioma is relatively small. And chemo- therapy is not ideal to the disease, either. Therefore, studies are needed towards finding radio-resistanc...Because of its low sensitivity to radiations, therapeutic ratio of neuroglioma is relatively small. And chemo- therapy is not ideal to the disease, either. Therefore, studies are needed towards finding radio-resistance gene. Human cerebral glioma SHG-44, mouse cerebral glioma C6, and small cell lung cancer NCI-H446 (for compari- son), were irradiated by Co γ-rays to 1, 3 and 8Gy, and changes of DNA/RNA ratios in the cells were studied 60 with LSCM (laser scanning confocal microscopy). It was found that the fluorescence dye of DNA in three kinds of cells in the control group is significantly higher than the RNA fluorescence dye (p<0.01). Degree of the damages to the irradiated NCI-H446 cells increased gradually with the dose, and damages to the cerebral glioma cells was less severe at the same radiation dose as the NCI-H446 cells.展开更多
目的通过体外细胞培养探讨粘附分子CD44在成骨细胞及破骨样细胞形成过程中的作用。方法在体外培养的骨髓间充质干细胞向成骨细胞分化过程中,用免疫细胞化学方法来检测其细胞CD44的表达及其意义;并在细胞核因子kB受体活化因子配基(recept...目的通过体外细胞培养探讨粘附分子CD44在成骨细胞及破骨样细胞形成过程中的作用。方法在体外培养的骨髓间充质干细胞向成骨细胞分化过程中,用免疫细胞化学方法来检测其细胞CD44的表达及其意义;并在细胞核因子kB受体活化因子配基(receptor activator of nuclear factor-k B ligand,RANKL)和巨噬细胞集落刺激因子(Macrophage-colony stimulating factor,M-CSF)诱导的破骨样细胞培养体系中加入CD44抗体,通过抗酒石酸酸性磷酸酶(TRAP)染色鉴定破骨样细胞的形成。结果成骨细胞培养体系中,空白组及地塞米松干预组间细胞CD44的表达没有显著性差异,但是指数增长期,地塞米松干预组的CD44的表达上升慢;破骨样细胞培养体系中,第6和9天,CD44抗体干预组的TRAP阳性细胞率明显低于对照组。结论 CD44在成骨细胞形成、增殖、成熟过程没有促进作用,但在破骨样细胞形成过程中起着一定的促进作用,它与单个核细胞的融合有一定的关系。展开更多
文摘Because of its low sensitivity to radiations, therapeutic ratio of neuroglioma is relatively small. And chemo- therapy is not ideal to the disease, either. Therefore, studies are needed towards finding radio-resistance gene. Human cerebral glioma SHG-44, mouse cerebral glioma C6, and small cell lung cancer NCI-H446 (for compari- son), were irradiated by Co γ-rays to 1, 3 and 8Gy, and changes of DNA/RNA ratios in the cells were studied 60 with LSCM (laser scanning confocal microscopy). It was found that the fluorescence dye of DNA in three kinds of cells in the control group is significantly higher than the RNA fluorescence dye (p<0.01). Degree of the damages to the irradiated NCI-H446 cells increased gradually with the dose, and damages to the cerebral glioma cells was less severe at the same radiation dose as the NCI-H446 cells.
文摘目的通过体外细胞培养探讨粘附分子CD44在成骨细胞及破骨样细胞形成过程中的作用。方法在体外培养的骨髓间充质干细胞向成骨细胞分化过程中,用免疫细胞化学方法来检测其细胞CD44的表达及其意义;并在细胞核因子kB受体活化因子配基(receptor activator of nuclear factor-k B ligand,RANKL)和巨噬细胞集落刺激因子(Macrophage-colony stimulating factor,M-CSF)诱导的破骨样细胞培养体系中加入CD44抗体,通过抗酒石酸酸性磷酸酶(TRAP)染色鉴定破骨样细胞的形成。结果成骨细胞培养体系中,空白组及地塞米松干预组间细胞CD44的表达没有显著性差异,但是指数增长期,地塞米松干预组的CD44的表达上升慢;破骨样细胞培养体系中,第6和9天,CD44抗体干预组的TRAP阳性细胞率明显低于对照组。结论 CD44在成骨细胞形成、增殖、成熟过程没有促进作用,但在破骨样细胞形成过程中起着一定的促进作用,它与单个核细胞的融合有一定的关系。
