Although many studies have found that cadmium(Cd)can be toxic to microalgae,only a few reports focused on the role of extracellular polymeric substances(EPS)in Cd(Ⅱ)detoxification.The biochemical and physiological en...Although many studies have found that cadmium(Cd)can be toxic to microalgae,only a few reports focused on the role of extracellular polymeric substances(EPS)in Cd(Ⅱ)detoxification.The biochemical and physiological endpoints of Microcystis aeruginosa,including the composition and functional groups of soluble EPS(SL-EPS),loosely bound EPS(LB-EPS),and tightly bound EPS(TB-EPS),were detected to elucidate the toxicity and detoxification mechanisms of Cd(Ⅱ)for cyanobacteria.Toxicological and physiological assays on M.aeruginosa showed that the 0.25-mg/L Cd(Ⅱ)resulted in a larger inhibition on growth and F_(v)/F_(m).Nevertheless,Cd(Ⅱ)significantly induced much higher contents of superoxide dismutase(SOD),intracellular microcystin LR(MC-LR),extracellular MC-LR,and EPS.Scanning electron microscopy with energy dispersive X-ray spectroscopy confirmed that Cd(Ⅱ)was absorbed into the EPS layer.Fourier transform infrared spectrum analysis revealed that the functional groups bound with Cd(Ⅱ)of algae biomass,SL-EPS,LB-EPS,and TB-EPS were somewhat different.The C=O/C=N groups ofδ-lactam or protein were their prominent functional groups,suggesting that amide or proteins in the EPS played a key role in the adsorption in Cd(Ⅱ).The concentration of 0.25 mg/L of Cd(Ⅱ)may change the chemical structure of EPS by altering the production of protein-like substances containing tryptophan.This study indicated that M.aeruginosa could detoxify Cd(Ⅱ)stress via induction of antioxidant capacity(higher SOD activity and MC synthesis),EPS production,and modification in chemical structure of EPS.展开更多
The seismic performance of a caisson structure under two types of models with a saturated sandy foundation(CSS)and an expanded polystyrene(EPS)composite soil foundation(CES)are studied using shaking table tests.The ma...The seismic performance of a caisson structure under two types of models with a saturated sandy foundation(CSS)and an expanded polystyrene(EPS)composite soil foundation(CES)are studied using shaking table tests.The macro phenomena of the two different foundation models are described and analyzed.The effects of the replacement of EPS composite soil on seismic-induced liquefaction of backfill and the dynamic performance of a caisson structure are evaluated in detail.The results show that the excess pore water pressure generation in the CES is significantly slower than that in the CSS during the shaking.The dynamic earth pressure acting on the caisson has a triangular shape.The response of horizontal acceleration,displacement,settlement,and rotation angle of the caisson in the CES is smaller than that in the CSS,which means the caisson in the CES has a better seismic performance.Furthermore,the out-of-phase phenomenon between dynamic earth thrust and inertial force in the CES is more obvious than that in the CSS,which is beneficial to reduce the lateral force and improve the stability of the caisson structure.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.31800457,32170383)。
文摘Although many studies have found that cadmium(Cd)can be toxic to microalgae,only a few reports focused on the role of extracellular polymeric substances(EPS)in Cd(Ⅱ)detoxification.The biochemical and physiological endpoints of Microcystis aeruginosa,including the composition and functional groups of soluble EPS(SL-EPS),loosely bound EPS(LB-EPS),and tightly bound EPS(TB-EPS),were detected to elucidate the toxicity and detoxification mechanisms of Cd(Ⅱ)for cyanobacteria.Toxicological and physiological assays on M.aeruginosa showed that the 0.25-mg/L Cd(Ⅱ)resulted in a larger inhibition on growth and F_(v)/F_(m).Nevertheless,Cd(Ⅱ)significantly induced much higher contents of superoxide dismutase(SOD),intracellular microcystin LR(MC-LR),extracellular MC-LR,and EPS.Scanning electron microscopy with energy dispersive X-ray spectroscopy confirmed that Cd(Ⅱ)was absorbed into the EPS layer.Fourier transform infrared spectrum analysis revealed that the functional groups bound with Cd(Ⅱ)of algae biomass,SL-EPS,LB-EPS,and TB-EPS were somewhat different.The C=O/C=N groups ofδ-lactam or protein were their prominent functional groups,suggesting that amide or proteins in the EPS played a key role in the adsorption in Cd(Ⅱ).The concentration of 0.25 mg/L of Cd(Ⅱ)may change the chemical structure of EPS by altering the production of protein-like substances containing tryptophan.This study indicated that M.aeruginosa could detoxify Cd(Ⅱ)stress via induction of antioxidant capacity(higher SOD activity and MC synthesis),EPS production,and modification in chemical structure of EPS.
基金National Natural Science Foundation of China under Grant Nos. 52178336 and 52108324Natural Science Research Project of Colleges and Universities in Jiangsu Province of China under Grant No. 18KJA560002+1 种基金the Middle-Aged&Young Science Leaders of Qinglan Project of Universities in Jiangsu Province of ChinaPostgraduate Research&Practice Innovation Program in Jiangsu Province of China under Grant No. KYCX24_1585
文摘The seismic performance of a caisson structure under two types of models with a saturated sandy foundation(CSS)and an expanded polystyrene(EPS)composite soil foundation(CES)are studied using shaking table tests.The macro phenomena of the two different foundation models are described and analyzed.The effects of the replacement of EPS composite soil on seismic-induced liquefaction of backfill and the dynamic performance of a caisson structure are evaluated in detail.The results show that the excess pore water pressure generation in the CES is significantly slower than that in the CSS during the shaking.The dynamic earth pressure acting on the caisson has a triangular shape.The response of horizontal acceleration,displacement,settlement,and rotation angle of the caisson in the CES is smaller than that in the CSS,which means the caisson in the CES has a better seismic performance.Furthermore,the out-of-phase phenomenon between dynamic earth thrust and inertial force in the CES is more obvious than that in the CSS,which is beneficial to reduce the lateral force and improve the stability of the caisson structure.
