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Olive Leaf Disease Detection via Wavelet Transform and Feature Fusion of Pre-Trained Deep Learning Models
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作者 Mahmood A.Mahmood Khalaf Alsalem 《Computers, Materials & Continua》 SCIE EI 2024年第3期3431-3448,共18页
Olive trees are susceptible to a variety of diseases that can cause significant crop damage and economic losses.Early detection of these diseases is essential for effective management.We propose a novel transformed wa... Olive trees are susceptible to a variety of diseases that can cause significant crop damage and economic losses.Early detection of these diseases is essential for effective management.We propose a novel transformed wavelet,feature-fused,pre-trained deep learning model for detecting olive leaf diseases.The proposed model combines wavelet transforms with pre-trained deep-learning models to extract discriminative features from olive leaf images.The model has four main phases:preprocessing using data augmentation,three-level wavelet transformation,learning using pre-trained deep learning models,and a fused deep learning model.In the preprocessing phase,the image dataset is augmented using techniques such as resizing,rescaling,flipping,rotation,zooming,and contrasting.In wavelet transformation,the augmented images are decomposed into three frequency levels.Three pre-trained deep learning models,EfficientNet-B7,DenseNet-201,and ResNet-152-V2,are used in the learning phase.The models were trained using the approximate images of the third-level sub-band of the wavelet transform.In the fused phase,the fused model consists of a merge layer,three dense layers,and two dropout layers.The proposed model was evaluated using a dataset of images of healthy and infected olive leaves.It achieved an accuracy of 99.72%in the diagnosis of olive leaf diseases,which exceeds the accuracy of other methods reported in the literature.This finding suggests that our proposed method is a promising tool for the early detection of olive leaf diseases. 展开更多
关键词 olive leaf diseases wavelet transform deep learning feature fusion
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Protective effects of oleic acid and polyphenols in extra virgin olive oil on cardiovascular diseases
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作者 Yan Lu Jun Zhao +6 位作者 Qiqi Xin Rong Yuan Yu Miao Manli Yang Hui Mo Keji Chen Weihong Cong 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期529-540,共12页
The Mediterranean diet has long been recognized as one of the most effective ways to prevent and improve cardiovascular disease.Extra virgin olive oil(EVOO)is the typical sources of fat in the Mediterranean diet which... The Mediterranean diet has long been recognized as one of the most effective ways to prevent and improve cardiovascular disease.Extra virgin olive oil(EVOO)is the typical sources of fat in the Mediterranean diet which have been shown to have noteworthy nutritional value and positive impact on human health.It is worth noting that EVOO owes its superior nutritional value to its bioactive composition.The main component of EVOO is monounsaturated fatty acids(MUFAs)in the form of oleic acid.Oleic acid accounts for up to 70%-80%of EVOO.Secondly,EVOO contains approximately more than 30 phenolic compounds,of which HT is essential for the protection against cardiovascular diseases.In this review,we focused on the potential mechanisms of oleic acid and polyphenols combat cardiovascular diseases risk in terms of oxidative stress,inflammation,blood pressure,endothelial function and cholesterol.This review might provide a reference for the studies on cardiovascular protective effects of EVOO. 展开更多
关键词 Cardiovascular diseases Extra virgin olive oil Oleic acid POLYPHENOLS
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Thermal Decomposition of Olive-Solid Waste by TGA: Characterization and Devolatilization Kinetics under Nitrogen and Oxygen Atmospheres
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作者 Yahya H. Khraisha 《Journal of Power and Energy Engineering》 2024年第3期31-47,共17页
Despite the fact that a few countries in the Mediterranean and the Middle East have limited crude oil reserves, they have abundant biomass feedstocks. For instance, Jordan relies heavily on the importation of natural ... Despite the fact that a few countries in the Mediterranean and the Middle East have limited crude oil reserves, they have abundant biomass feedstocks. For instance, Jordan relies heavily on the importation of natural gas and crude oil for its energy needs;but, by applying thermochemical conversion techniques, leftover olive oil can be used to replace these energy sources. Understanding the chemical, physical, and thermal characteristics of raw materials is essential to obtaining the most out of these conversion processes. Thermogravimetric analysis was used in this study to examine the thermal behavior of olive-solid residue (kernel) at three different heating rates (5, 20 and 40 C/min) in nitrogen and oxygen atmospheres. The initial degradation temperature, the residual weight at 500 and 700˚C and the thermal degradation rate during the devolatilization stage (below 400˚C) were all determined. It was found that in N<sub>2</sub> and O<sub>2</sub> atmospheres, both the initial degradation temperature and the degradation rate increase with increasing heating rates. As heating rates increase in the N<sub>2</sub> atmosphere, the residual weight at 500 or 700˚C decreases slightly, but at low heating rates compared to high heating rates in the O<sub>2</sub> atmosphere, it decreases significantly. This suggests that a longer lignin oxidation process is better than a shorter one. Coats and Redfern approach was used to identify the mechanism and activation energy for the devolatilization stage of pyrolysis and oxidation reactions. The process mechanism analysis revealed that the model of first-order and second-order reactions may adequately describe the mechanism of heat degradation of the devolatilization step of olive-solid waste for pyrolysis and oxidation processes, respectively. 展开更多
关键词 Biomass olive-Solid Waste THERMOGRAVIMETRY Pyrolysis Oxidation Heating Rates Kinetics
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Production of Biogas from Olive Mill Waste Waters Treated by Cow Manure
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作者 Fatma Laabidi Rafik Besbès Bassem Jamoussi 《Green and Sustainable Chemistry》 CAS 2023年第1期62-71,共10页
The olive mill waste waters (OMWW) generated from olive oil extraction is a major environmental concern since they are characterized by their role as a pollutant (high organic and mineral matters) and their pH acid. T... The olive mill waste waters (OMWW) generated from olive oil extraction is a major environmental concern since they are characterized by their role as a pollutant (high organic and mineral matters) and their pH acid. The aim of this study was to valorize (OMWW) by anaerobic fermentation in the presence of cow manure in order to produce biogas and reduce their toxic load. Many tests were carried out by fermenting (OMWW) with polyphenols in the presence of cow manure in thermophile temperatures. The performance of this treatment was valuated through measurements of biogas production and by the determination of different parameters of fermented media (pH, volume of the biogas and polyphenols). 展开更多
关键词 olive Mill Waste Waters Anaerobic Fermentation Cow Manure BIOGAS POLYPHENOLS
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Olive oil ameliorate allergic response in ovalbumin-induced food allergy mouse by promoting intestinal mucosal immunity
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作者 Yu Ma Ming Liu +5 位作者 Donghui Li Jie Li Zixin Guo Yunjun Liu Shengnan Wan Yixiang Liu 《Food Science and Human Wellness》 SCIE CSCD 2023年第3期801-808,共8页
The numerous health benefits of olive oil are widely known,however,it also provides anti-allergic properties that have not yet been fully defined.In this study,the anti-allergic activity of olive oil was evaluated by ... The numerous health benefits of olive oil are widely known,however,it also provides anti-allergic properties that have not yet been fully defined.In this study,the anti-allergic activity of olive oil was evaluated by analyzing the clinical symptoms and immune-related factors in BALB/c mice that had ingested600 mg/(kg·day)olive oil for two weeks prior to the evaluation.An allergy model was subsequently constructed for analysis,the results of which showed that the olive oil reduced the scores of allergic symptoms in the mice,and up-regulated the hypothermia and the decline in the immune organ index.Moreover,fewer allergy-related cytokines and reduced intestinal inflammation was discovered in the olive oil-treated group.In addition,analysis of intestinal mucosal immune-related factors revealed that the olive oil promoted the expression of intestinal tight junction proteins(Claudin-1,Occludin,and ZO-1)and IL-22,and helped maintain the integrity of the intestinal epithelial physical barrier.Increased levels of mucin 2 andβ-defensin were also found in the intestinal mucus of the olive oil-treated mice.These findings suggest that the oral administration of olive oil effectively attenuated the ovalbumin-induced allergic immune response in the mice,and had a positive effect on intestinal epithelial mucosal immunity. 展开更多
关键词 olive oil Ovalbumin-induced food allergy Anti-food allergy Intestinal mucosal immunization
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Chimonanthus nitens Oliv. leaves flavonoids alleviate hyperuricemia by regulating uric acid metabolism and intestinal homeostasis in mice
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作者 Wenya Meng Lingli Chen +4 位作者 Kehui Ouyang Suyun Lin Yang Zhang Jing He Wenjun Wang 《Food Science and Human Wellness》 SCIE CSCD 2023年第6期2440-2450,共11页
This study aimed to evaluate the alleviating effect of Chimonanthus nitens Oliv.leaves flavonoids(CLF)on hyperuricemia induced by potassium oxonate in mice.The results showed that CLF lowered the serum levels of uric ... This study aimed to evaluate the alleviating effect of Chimonanthus nitens Oliv.leaves flavonoids(CLF)on hyperuricemia induced by potassium oxonate in mice.The results showed that CLF lowered the serum levels of uric acid(UA),creatinine and blood urea nitrogen,downregulated hepatic mRNA expressions of xanthine oxidase(XO),phosphate ribose pyrophosphate synthetase(PRPS)and adenosine deaminase(ADA)in hyperuricemia mice.In addition,CLF repaired renal injury by significantly down-regulating mRNA and protein expressions of renal UA reabsorption-related proteins and up-regulating the mRNA and protein expressions of UA secretory-related proteins.Finally,CLF inhibited UA synthesis and promoted UA excretion to alleviate hyperuricemia.Besides,CLF supplementation repaired the intestinal barrier function as demonstrated by significant increased mRNA levels of intestinal zonula occludens-1(ZO-1),Occludin,mucin 2(MUC2)and mucin 4(MUC4),as well as decreased mRNA levels of toll-like receptor 4(TLR4)and myeloid differentiation factor 88(MyD88)in mice.Further research showed that CLF treatment restored intestinal homeostasis mediated by improving the composition of gut microbiota and elevating the abundance of beneficial bacteria like Lactobacillus,Alistipes,Prevotellaceae_UCG-001 and Parasutterella.Overall,our findings revealed a novel function of CLF as a promising therapeutic candidate for the treatment of hyperuricemia. 展开更多
关键词 Chimonanthus nitens oliv.leaves FLAVONOIDS HYPERURICEMIA Renal damage Gut microbiota
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Nesting Biology and Site Selection of Olive Ridley—A Coherence of Nature
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作者 Arun Malarvizhi P. M. Mohan 《Open Journal of Marine Science》 2023年第2期29-39,共11页
Nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. In the present study, characteristics of the breeding turtle, Olive Ridley (Lepidochely... Nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. In the present study, characteristics of the breeding turtle, Olive Ridley (Lepidochelys olivacea) at Ramnagar along N-E coast of North Andaman Islands were investigated, for the nesting periods 2016-2017. Olive Ridley is the dominant sea turtles, with more than 300 individuals nesting every season with at least a 75% hatching success rate. It is one of the conducive, highly protective and undisturbed nesting sites for Olive Ridley on North Andaman coast. In the present study an initiative is made, to identify nature’s cues and the biological characteristics of Olive Ridley, which leads it to nest at Ramnagar beach, with a high hatchling success rate. 展开更多
关键词 Sea Turtle olive Ridley Ramnagar Beach Nesting Beach Sand Grain Hatch-ling North Andaman
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The Probable Cause for Nesting Pattern of Olive Ridley (Lepidochelys olivacea) at Ramnagar Beach, North East Coast of Andaman Island, India
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作者 Arun Malarvizhi M. M. Ilaamurughu P. M. Mohan 《Open Journal of Marine Science》 2023年第1期7-27,共21页
The nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. Over a period of time, several reports have been made in this context associated with th... The nesting behaviour of sea turtles remains a subject to study, due to their enigmatic pattern of seasonal breeding activities. Over a period of time, several reports have been made in this context associated with the nesting behaviour of the Olive Ridley turtles. In the present study, characteristics of the breeding beach and nesting pattern of Olive Ridley (Lepidochelys olivacea) at Ramnagar along N-E coast of Andaman Islands were investigated, during the nesting periods 2016-2017. The study area hosts Olive Ridley, the dominant sea turtles with more than 300 individuals nesting each year. For this study, the number of sea turtles visited, nested, the sediment characters, salinity, and temperature were taken. The exposed sandy nesting beach characteristics are prone to varying degrees of morphological changes every day. The results depict that even though similar grain size (Coarse Sand to Fine Sand and Very well sorted to Poorly Sorted), with an ambient incubating temperature, pH and salinity with wide nesting area, the selective nesting in the particular location of the beach identified because of comfortable energy conditions in the waters (1.5 m/s) favours the female turtles to reach the beach at the preferable site of Ramnagar and nest. 展开更多
关键词 Sea turtle olive Ridley Ramnagar Beach Nesting Environment Morphological Changes Sand Grain HATCHLING North Andaman
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Integrated Use of Organic and Bio-fertilizers to Improve Yield and Fruit Quality of Olives Grown in Low Fertility Sandy Soil in an Arid Environment
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作者 Bassam F.Alowaiesh M.M.Gad Mohamed Saleh M.Ali 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第6期1813-1829,共17页
Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers o... Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers on the physiological growth,yield and fruit quality of olive trees under newly reclaimed poor-fertility sandy soil in an arid environment.During a field experiment carried out at El-Qantara,North Sinai,Egypt over two consecutive seasons(2019-2020 and 2020-2021),olive Kalamata trees were evaluated under three organic fertilizer treatments alone or in combination with three bio-fertilizers treatments.Organic fertilizer was applied as goat manure(16.8 kg/tree/year),or olive pomace(8.5 kg/tree/year)in mid-December of each season vs.untreated trees.The bio-fertilizers were applied as N-fixing bacteria(150 g/tree)was inculated in early March of each season,or amino acid mixture(1.5%)was applied three times,at 70%of full bloom,21 days after full bloom,and a month later in comparison to a non-fertilized trees(control).The cultivar used was Kalamata,a dual-purpose cultivar for oil and table olives whose value increases when processed as table olives.The results indicated that the goat manure followed by olive pomace significantly enhanced photosynthetic pigments(chlorophyll a,b,and carotenoids),leaf mineral contents(N,P,K,Ca,Mg and Fe),tree canopy volume,number of flowers per inflorescence,number of inflorescences per shoot,initial fruit set,fruit retention.For fruit quality,fruit length and width,fruit weight,and total fruit yield was increased compared to the non-fertilized control.Likewise,The bio-fertilizer N-fixing bacteria followed by the amino acid mixture significantly improved all of the aforementioned parameters.Accordingly,it is recommended,both environmentally and economically to utilize organic and bio-fertizers,particularly goat manure combined with N-fixing bacteria,in low-fertility soil to sustain olive production as well as reducing mineral fertilization. 展开更多
关键词 Organic and bio-fertilizers olivES kalamata vegetative growth leaf mineral contents fruit quality
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超声波辅助橄榄油提取大麻二酚工艺研究
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作者 高宝昌 石雨 +7 位作者 田媛 李国巍 李纯思 张正海 王义志 张治国 徐海鑫 孙宇峰 《黑龙江科学》 2024年第8期6-9,13,共5页
以工业大麻花叶为原料,采用超声波辅助橄榄油提取大麻二酚(CBD),利用中心组合(CCD)方法对提取工艺的4种因素进行优化。实验结果表明,最佳提取条件为:超声波功率95 W、提取温度62℃、提取时间45 min、料液比1:9(g/mL),该条件下CBD浓度为3... 以工业大麻花叶为原料,采用超声波辅助橄榄油提取大麻二酚(CBD),利用中心组合(CCD)方法对提取工艺的4种因素进行优化。实验结果表明,最佳提取条件为:超声波功率95 W、提取温度62℃、提取时间45 min、料液比1:9(g/mL),该条件下CBD浓度为39.01μg/mL,提取率达到91.35%,此结果为CBD的多元化开发应用奠定了技术基础。 展开更多
关键词 工业大麻 大麻二酚 橄榄油 响应面法
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不同种植年限川党参根际土壤细菌群落结构研究
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作者 罗敏 朱晓富 +5 位作者 徐广 罗舜 卢胜鄂 郭瑀 杨丹 邓才富 《中国土壤与肥料》 CAS CSCD 北大核心 2024年第3期32-39,共8页
为了探究生长年限对川党参根际土壤细菌群落结构特征及其多样性的影响,阐明细菌群落变化规律,采用Illumina MiSeq高通量测序技术对1年生、2年生、3年生和4年生的川党参根际土壤细菌16S rRNA序列进行测序分析。结果表明:(1)生长年限显著... 为了探究生长年限对川党参根际土壤细菌群落结构特征及其多样性的影响,阐明细菌群落变化规律,采用Illumina MiSeq高通量测序技术对1年生、2年生、3年生和4年生的川党参根际土壤细菌16S rRNA序列进行测序分析。结果表明:(1)生长年限显著影响川党参根际土壤细菌群落多样性,其OTU数、Alpha多样性指数均随生长年限增加呈先升高后降低的趋势;(2)生长年限改变了细菌群落组成,但在门水平上优势菌门(相对丰度>5%)相对稳定,均为变形菌门(Proteobacteria)、未分类细菌门(unclassified_Bacteria)、酸杆菌门(Aci-dobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)和绿弯菌门(Chloroflexi),其相对丰度总和占85.71%~91.39%;在属水平上优势菌群相差较大,仅有全噬菌属(Holophaga)和念珠菌属(Candidatus Udaeo-bacter)是4个年限所共有的优势菌属,革兰氏阴性菌cTPY-13(Gram-negative bacterium cTPY-13)则为2年生独有优势菌属,其余则分别为不同年限有相应共有优势菌属;(3)PCoA分析与UPGMA聚类分析显示,各生长年限间细菌群落差异明显,随生长年限增加,不同组间细菌群落结构差异先增大后逐渐减小,细菌群落在2~3年生时演变较为剧烈;(4)土壤速效养分是影响根际土壤细菌群落的主要环境因子,其程度大小为水解氮(HN)>有效磷(AP)>速效钾(AK),优势菌门中酸杆菌门与AP、AK呈显著负相关(P<0.05),拟杆菌门与AP、AK呈显著正相关(P<0.05),而绿弯菌门与HN呈显著负相关(P<0.05)。综上所述,川党参生长年限延长会影响根际土壤细菌群落结构和多样性,从而改变其根际微生态系统。 展开更多
关键词 川党参 根际土壤 细菌群落 高通量测序
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橄榄仁抗氧化肽的分离鉴定及分子对接
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作者 张迎阳 董亚云 +1 位作者 邹平 郑文武 《食品与发酵工业》 CAS CSCD 北大核心 2024年第5期189-198,共10页
该研究利用酶解法结合凝胶色谱柱从橄榄仁中分离纯化得到6个抗氧化肽,采用分子对接模拟6种抗氧化肽与超氧化物歧化酶(superoxide dismutase, SOD)的结合机制。采用酶解法从橄榄仁中提取抗氧化活性肽,以水解度、DPPH自由基清除率为指标,... 该研究利用酶解法结合凝胶色谱柱从橄榄仁中分离纯化得到6个抗氧化肽,采用分子对接模拟6种抗氧化肽与超氧化物歧化酶(superoxide dismutase, SOD)的结合机制。采用酶解法从橄榄仁中提取抗氧化活性肽,以水解度、DPPH自由基清除率为指标,从6种商用酶中筛选出最适蛋白酶为木瓜蛋白酶,以底物浓度、酶底比、酶解时间、温度进行单因素试验,选取影响酶解反应的3个因素(酶底比>温度>时间)进行响应面法优化得出最佳工艺条件为底物质量浓度50 g/L、酶底比为3.3%(质量分数)、温度20℃、时间88 min。对最优条件下的粗肽液进行SephadexG-25和SephadexG-15分离纯化得到6个具有良好活性的抗氧化肽,分别为WLSF、TSVLR、LVYLVR、AGGKPFQ、YYPFKGFA、AFNVDERLAR。TSVLR显示出最高的DPPH自由基清除活性81.07%和羟自由基清除能力42.48%。分子对接研究表明,纯化的肽通过疏水效应和氢键与抗氧化相关的关键蛋白SOD有效结合。结果表明,橄榄仁可作为天然食物来源制取功能性食品的一个新的探究方向。 展开更多
关键词 橄榄仁 抗氧化肽 响应面优化 分离鉴定 分子对接
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响应面结合模糊数学感官评价法优化油橄榄果渣曲奇饼干的配方及其品质测定
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作者 樊亮 叶文斌 +3 位作者 胡文斌 杨文 孙娜 王林林 《甘肃农业大学学报》 CAS CSCD 北大核心 2024年第2期282-295,共14页
【目的】开发油橄榄果渣曲奇饼干。【方法】以低筋面粉、黄油和鲜鸡蛋为材料复配陇南油橄榄果渣、麦芽糖醇、金耳和纹党多糖,添加碳酸氢钠和氯化钠等食品添加剂研制油橄榄果渣低糖复合曲奇饼干,采用响应面结合模糊数学感官评价法优化了... 【目的】开发油橄榄果渣曲奇饼干。【方法】以低筋面粉、黄油和鲜鸡蛋为材料复配陇南油橄榄果渣、麦芽糖醇、金耳和纹党多糖,添加碳酸氢钠和氯化钠等食品添加剂研制油橄榄果渣低糖复合曲奇饼干,采用响应面结合模糊数学感官评价法优化了配方,对其抗氧化性、理化指标和微生物指标进行了检验。【结果】油橄榄果渣添加量为15%、麦芽糖醇添加量为30%、金耳添加量为10%和纹党多糖添加量为0.35%,曲奇饼干形态完整,断面结构细密、无粉末,硬度适中,薄厚均匀、口感细腻、咀嚼性好,酥脆、无颗粒感,油橄榄风味突出,气味协调、颜色均一卫生指标符合国家标准,具有很好的营养价值和抗氧化功能。【结论】为油橄榄果渣的进一步利用提供了参考。 展开更多
关键词 油橄榄果渣 曲奇饼干 配方优化 响应面试验 模糊数学法 抗氧化
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种苗期川党参根际土壤细菌群落变化研究
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作者 罗敏 邓才富 +2 位作者 朱晓富 徐广 罗舜 《亚太传统医药》 2024年第1期51-55,共5页
目的:研究川党参种苗期根际土壤细菌群落的结构特征和多样性变化,掌握种苗期土壤微生物群落的演替规律,为川党参连作障碍的防治提供理论依据。方法:采集1年生(Op1)和2年生(Op2)的川党参根际土壤,采用Illumina MiSeq高通量测序技术进行细... 目的:研究川党参种苗期根际土壤细菌群落的结构特征和多样性变化,掌握种苗期土壤微生物群落的演替规律,为川党参连作障碍的防治提供理论依据。方法:采集1年生(Op1)和2年生(Op2)的川党参根际土壤,采用Illumina MiSeq高通量测序技术进行细菌16S rRNA序列V3-V4区的高通量测序,对种苗期根际土壤细菌群落变化进行分析。结果:通过MiSeq测序共得到882369条优化序列,其中1年生注释到9926个OTU,2年生注释到7560个OTU。种苗期随生长年限的增加,川党参根际土壤速效养分、细菌群落丰富度和多样性均呈增加趋势。在细菌门分类水平上,1年生有5个优势菌群(相对丰度均大于5%),2年生有7个优势菌群,共有优势菌群为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes);在属分类水平上排名前五菌群中只有全噬菌属(Holophaga)与念珠菌属(Candidatus Udaeobacter)为共有优势菌群,且1年生有11个独有菌群。PCoA分析与UPGMA聚类分析表明1年生与2年生川党参根际土壤细菌群落具有显著差异,随生长年限延长根际土壤细菌群落结构演变明显。结论:川党参种苗期根际土壤速效养分、土壤细菌群落多样性呈递增变化,细菌群落结构相似性呈递减变化。研究为揭示川党参种植和连作障碍形成的原因提供了理论依据。 展开更多
关键词 川党参 根际土壤 细菌群落 种苗期
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川党参的化学成分及其与磷酸二酯酶4D2的分子对接研究
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作者 刘新桥 胡贝 +3 位作者 袁桥玉 宋炜 杨中正 吴娅 《中南民族大学学报(自然科学版)》 CAS 2024年第2期175-180,共6页
采用硅胶柱色谱、葡聚糖凝胶以及反相高效液相色谱法等对川党参的乙酸乙酯和石油醚部位进行分离纯化,并通过^(1)H NMR和^(13)C NMR等波谱技术进行结构鉴定.利用Sybyl X 2.1.1软件,以Total score值为评价标准,评价川党参化学成分与磷酸... 采用硅胶柱色谱、葡聚糖凝胶以及反相高效液相色谱法等对川党参的乙酸乙酯和石油醚部位进行分离纯化,并通过^(1)H NMR和^(13)C NMR等波谱技术进行结构鉴定.利用Sybyl X 2.1.1软件,以Total score值为评价标准,评价川党参化学成分与磷酸二酯酶4D2(PDE4D2)的结合作用.利用Pymol和Discovery Studio 2017 R2 Client软件分析PDE4D2与化合物之间的相互作用力.结果从乙酸乙酯和石油醚部位分离出12个化合物,分别鉴定为mangnostellin D(1)、(7S,8S)-3-methoxy-3',7-epoxy-8,4'-oxyneoligna-4,9,9,'-triol(2)、isolariciresinol(3)、curcasinlignan B(4)、(4E,12Z)-threo-tetradeca-4,12-diene-8,10-diyne-1,6,7,14-tetraol(5)、(-)-(7R,8S,7'E)-3',4-dihydroxy-3-methoxy-8,4'-oxyneoligna-7'-ene-7,9,9'-triol(6)、tetradeca-4E,8E,12E-triene-10-yne-1,6,7-triol(7)、lobetyol(8)、1-α-linoleoylglycerol(9)、glycerol 1-(9,12,15-octadecatrienoate)(10)、3,5-二甲氧基-4-羟基-苯丙酮(11)、syringic acid ethyl ester(12);其中化合物1、4、10、11和12为首次从该植物中分离得到;分子对接结果表明化合物1、2、5和化合物6与PDE4D2有较好的结合活性. 展开更多
关键词 川党参 化学成分 分子对接 磷酸二酯酶4D2 抗炎
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基于PCA-SVM结合共聚焦拉曼光谱的特级初榨橄榄油掺伪压榨菜籽油定量分析
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作者 彭楠 方俊 毛潭 《中国油脂》 CAS CSCD 北大核心 2024年第2期70-74,共5页
为了促进国内橄榄油市场的健康发展,对掺伪同样存在天然类胡萝卜素的低温压榨菜籽油的特级初榨橄榄油进行了定量鉴别研究。采用共聚焦拉曼光谱技术对不同掺伪浓度油样进行测试,基于密度泛函理论对油样的拉曼光谱峰的归属进行了理论分析... 为了促进国内橄榄油市场的健康发展,对掺伪同样存在天然类胡萝卜素的低温压榨菜籽油的特级初榨橄榄油进行了定量鉴别研究。采用共聚焦拉曼光谱技术对不同掺伪浓度油样进行测试,基于密度泛函理论对油样的拉曼光谱峰的归属进行了理论分析,并对拉曼光谱数据进行主成分分析(PCA),然后利用支持向量机(SVM)构建PCA-SVM模型。另外,对PCA-SVM模型的检出限进行了研究。结果表明:特级初榨橄榄油与低温压榨菜籽油的拉曼光谱存在一定差异,最明显的光谱差异主要集中在谱峰1008、1161、1528 cm^(-1)和谱段2800~3000 cm^(-1)内,与密度泛函理论对不同油样拉曼光谱峰的分析一致;不考虑类胡萝卜素特征信号建立的PCA-SVM模型决定系数大于0.989,均方根误差小于2.990%,检出限为2%(低温压榨菜籽油体积分数);在特级初榨橄榄油掺伪定量分析中,考虑类胡萝卜素的特征信号有助于提高模型预测精度,但仅限于掺伪低价植物油中无类胡萝卜素存在的情况;PCA-SVM模型在不考虑类胡萝卜素特征信号的情况下依然具有良好的定量预测效果。综上,所建立的PCA-SVM模型可以用于掺伪2%以上低温压榨菜籽油的特级初榨橄榄油的定量鉴别。 展开更多
关键词 特级初榨橄榄油 低温压榨菜籽油 PCA-SVM 拉曼光谱 密度泛函理论
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基于特级初榨橄榄油两种不同特性的DSC掺假分析方法研究
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作者 魏燕 宋志强 武卫东 《食品与发酵工业》 CSCD 北大核心 2024年第1期183-188,共6页
该文基于油脂物理特性与氧化稳定性2种特性,利用差示扫描量热法(differential scanning calorimetry,DSC)对市面上常见的特级初榨橄榄油(extra virgin olive oil,EVOO)中大豆油掺假问题进行研究。在物理特性方面,采用DSC冷却曲线来分析E... 该文基于油脂物理特性与氧化稳定性2种特性,利用差示扫描量热法(differential scanning calorimetry,DSC)对市面上常见的特级初榨橄榄油(extra virgin olive oil,EVOO)中大豆油掺假问题进行研究。在物理特性方面,采用DSC冷却曲线来分析EVOO掺假。随着大豆油掺假比例升高,油样在-40~-60℃结晶焓值ΔH由EVOO的29.78 J/g减小为40%掺假混合油的6.133 J/g,对EVOO质量比构建的一元回归方程Y=57.556X-28.44(R2=0.997)。在氧化稳定性方面,氧化温度160℃下,不同油样诱导氧化时间随大豆油掺假比例上升逐渐变短,由EVOO的25.8 min逐渐减小为40%掺假混合油的17.8 min,Y=-19.4X+25.64(R^(2)=0.996),掺假模型相关性较好。这2种DSC检验掺假分析方法都能有效鉴别EVOO中大豆油的掺假且各有特点,可根据实际情况选取合适的方法更好处理EVOO掺假问题。 展开更多
关键词 特级初榨橄榄油 大豆油 物理特性 氧化稳定性 差示扫描量热法 掺假鉴定
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油橄榄果渣的营养与功能特性及其在畜禽生产中的应用研究进展 被引量:2
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作者 韦良开 张安宁 +1 位作者 李瑞 印遇龙 《动物营养学报》 CAS CSCD 北大核心 2024年第1期1-11,共11页
油橄榄果渣是油橄榄榨油后的主要副产物,其粗脂肪、粗蛋白质、可溶性纤维、矿物质等营养成分含量丰富,且富含植物多酚、黄酮、环烯醚、甾醇等多种天然活性成分,有降血脂、抗氧化、抗菌、抗炎症等功效。油橄榄果渣作为畜禽饲料原料,不仅... 油橄榄果渣是油橄榄榨油后的主要副产物,其粗脂肪、粗蛋白质、可溶性纤维、矿物质等营养成分含量丰富,且富含植物多酚、黄酮、环烯醚、甾醇等多种天然活性成分,有降血脂、抗氧化、抗菌、抗炎症等功效。油橄榄果渣作为畜禽饲料原料,不仅可以变“废”为宝、降低饲料成本、减少环境污染,还能改善畜禽生长性能、增强机体免疫和抗氧化能力、提高畜禽产品品质。本文概述了油橄榄果渣的营养特点、生物学功能及其在畜禽中的应用现状,旨在为油橄榄果渣作为饲料原料的高效利用提供参考。 展开更多
关键词 油橄榄果渣 营养特性 生物学功能 畜牧业
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栽培方式对航天诱变当归SP_(2)繁种特性的影响
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作者 崔林刚 郭凤霞 +2 位作者 陈垣 徐波 李瑞霞 《核农学报》 CAS CSCD 北大核心 2024年第5期813-821,共9页
当归3年完成正常生活史,繁种周期长,成种株率低。为提高当归新品种选育效率,将搭载“长征7号”运载火箭在太空运行22 h(命名为航归22 h)和搭载“神舟十一号”宇宙飞船在太空运行33 d(命名为航归33 d)的当归种子作为SP0种子,以未搭载种... 当归3年完成正常生活史,繁种周期长,成种株率低。为提高当归新品种选育效率,将搭载“长征7号”运载火箭在太空运行22 h(命名为航归22 h)和搭载“神舟十一号”宇宙飞船在太空运行33 d(命名为航归33 d)的当归种子作为SP0种子,以未搭载种子为对照(CK),经育苗栽培获得SP_(2)种苗。采用留苗和移栽2种栽培方式,测定进入第3年繁种年的SP_(2)群体的种株率、农艺性状和种果重。结果表明,CK群体未能进入繁种期而被淘汰,太空诱变获得的2个群体均进入正常繁种年。航归22 h的返青株成活率较航归33 d提高14.65个百分点,成种株率提高19.7个百分点。与移栽航归33 d、留苗航归22 h和33 d群体相比较,移栽航归22 h的繁殖特性有明显改善,单株果重分别增加1527.5%、428.2%和377.7%,根重分别增加235.3%、256.8%和265.2%,根长分别增加44.5%、36.7%和6.0%,根粗分别增加83.3%、83.7%和83.9%。航归SP_(2)代群体性状综评指数大小依次为移栽航归22 h(0.8133)>留苗航归33 d(0.4083)>留苗航归22 h(0.2378)>移栽航归33 d(0.0334)。综上,太空搭载种子对当归具有诱变效应,运载火箭搭载时间短,群体诱变效应更佳,选育效率更高;采用移栽和留苗互补栽培可保障繁种的安全性。本研究结果为当归新品系扩繁提供了科学技术依据。 展开更多
关键词 当归 太空诱变 移栽 留苗 繁种
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榄仁黑豆沙月饼馅料配方优化
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作者 游云 黄晓霞 +4 位作者 刘巧瑜 易祚良 郑玲燕 曾晓房 陈海光 《粮食与饲料工业》 CAS 2024年第2期21-26,31,共7页
试验以黑豆、白砂糖、花生油、橄榄仁为原料,单甘酯、海藻酸钠为辅料,利用响应面法研究并优化榄仁黑豆沙月饼馅料的配方。结果表明,榄仁黑豆沙月饼馅料的最佳配方为:以黑豆沙质量计,白砂糖40%,花生油45%,橄榄仁16%,单甘酯0.8%,海藻酸钠0... 试验以黑豆、白砂糖、花生油、橄榄仁为原料,单甘酯、海藻酸钠为辅料,利用响应面法研究并优化榄仁黑豆沙月饼馅料的配方。结果表明,榄仁黑豆沙月饼馅料的最佳配方为:以黑豆沙质量计,白砂糖40%,花生油45%,橄榄仁16%,单甘酯0.8%,海藻酸钠0.4%。在此配方下的榄仁黑豆沙月饼馅料品质较好,外表有光泽,口感顺滑,甜味适中。橄榄仁与黑豆沙结合,使两者的保健功能和营养更加相辅相成。 展开更多
关键词 橄榄仁 黑豆 响应面 感官评价 月饼馅料
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