Objective:To study the clinical efficacy and effects of danzhi xiaoyao pill combined with metformin on related factors in patients with polycystic ovary syndrome (PCOS).Methods:A total of 80 patients with PCOS in our ...Objective:To study the clinical efficacy and effects of danzhi xiaoyao pill combined with metformin on related factors in patients with polycystic ovary syndrome (PCOS).Methods:A total of 80 patients with PCOS in our hospital from January 2014 to December 2017 were enrolled in this study. The subjects were divided into the control group (n=40) and the experimental group (n=40) randomly. The control group were treated with metformin, the experimental group were treated with danzhi xiaoyao pill combined with metformin, and the two groups were treated for 3 menstrual cycles. The serum CRP, IGF-1, TNF-α, FSH, LH, E2, T, FINS, FPG, VF, LEP, APN and HOMA-IR of the two groups before and after treatment were compared.Results: There were no significantly differences of the serum CRP, IGF-1, TNF-α, FSH, LH, E2, T, FINS, FPG, VF, LEP, APN and HOMA-IR of the two groups before treatment. After treatment, the serum CRP, IGF-1, TNF-α, LH, E2, T, FINS, FPG, VF, LEP and HOMA-IR of the two groups were significantly lower than before treatment, the serum APN of the two groups were significantly higher than before treatment, and that of the experimental group after treatment were significantly better than the control group. Conclusion: Danzhi xiaoyao pill combined with metformin on patients with PCOS has a good efficacy. It can reduce the inflammatory cytokines and sex hormones, and improve the insulin resistance and sugar, lipid metabolic disorders. Therefore, it is worthy of clinical application.展开更多
Objective:To investigate the effects of 50% ethyl alcohol(EtOH)extracts from Danzhi Xiaoyao Pill(丹栀逍遥丸,DXP)on the proliferation of MCF-7 human breast cancer cells and potential mechanisms.Methods:ATP-Lite assay w...Objective:To investigate the effects of 50% ethyl alcohol(EtOH)extracts from Danzhi Xiaoyao Pill(丹栀逍遥丸,DXP)on the proliferation of MCF-7 human breast cancer cells and potential mechanisms.Methods:ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line;and antioxidant activity was measured by the oxygen radical absorbance capacity (ORAC).The effects of DXP on nitric oxide(NO)production were tested by lipopolysaccharide(LPS)- stimulated RAW 264.7 murine macrophages using the Griess reaction.Results:The 50% EtOH DXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10,0.25 and 0.50 mg/mL dose- dependently with the proliferation inhibited by more than 85%.The ORAC value of the DXP was 820μmoL Trolox equivalent/g,about 40% of the vitamin C value.DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL(P<0.05,P<0.01).Conclusion:The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells,with the effect possibly related to its antioxidant activity and the inhibition of NO production.展开更多
文摘Objective:To study the clinical efficacy and effects of danzhi xiaoyao pill combined with metformin on related factors in patients with polycystic ovary syndrome (PCOS).Methods:A total of 80 patients with PCOS in our hospital from January 2014 to December 2017 were enrolled in this study. The subjects were divided into the control group (n=40) and the experimental group (n=40) randomly. The control group were treated with metformin, the experimental group were treated with danzhi xiaoyao pill combined with metformin, and the two groups were treated for 3 menstrual cycles. The serum CRP, IGF-1, TNF-α, FSH, LH, E2, T, FINS, FPG, VF, LEP, APN and HOMA-IR of the two groups before and after treatment were compared.Results: There were no significantly differences of the serum CRP, IGF-1, TNF-α, FSH, LH, E2, T, FINS, FPG, VF, LEP, APN and HOMA-IR of the two groups before treatment. After treatment, the serum CRP, IGF-1, TNF-α, LH, E2, T, FINS, FPG, VF, LEP and HOMA-IR of the two groups were significantly lower than before treatment, the serum APN of the two groups were significantly higher than before treatment, and that of the experimental group after treatment were significantly better than the control group. Conclusion: Danzhi xiaoyao pill combined with metformin on patients with PCOS has a good efficacy. It can reduce the inflammatory cytokines and sex hormones, and improve the insulin resistance and sugar, lipid metabolic disorders. Therefore, it is worthy of clinical application.
文摘Objective:To investigate the effects of 50% ethyl alcohol(EtOH)extracts from Danzhi Xiaoyao Pill(丹栀逍遥丸,DXP)on the proliferation of MCF-7 human breast cancer cells and potential mechanisms.Methods:ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line;and antioxidant activity was measured by the oxygen radical absorbance capacity (ORAC).The effects of DXP on nitric oxide(NO)production were tested by lipopolysaccharide(LPS)- stimulated RAW 264.7 murine macrophages using the Griess reaction.Results:The 50% EtOH DXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10,0.25 and 0.50 mg/mL dose- dependently with the proliferation inhibited by more than 85%.The ORAC value of the DXP was 820μmoL Trolox equivalent/g,about 40% of the vitamin C value.DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL(P<0.05,P<0.01).Conclusion:The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells,with the effect possibly related to its antioxidant activity and the inhibition of NO production.
