Menopausal metabolic syndrome(MMS) is a series of syndrome caused by ovarian function decline and hormone insufficiency, and is a high risk factor for cardiovascular diseases(CVD) and type II diabetes mellitus(T2DM). ...Menopausal metabolic syndrome(MMS) is a series of syndrome caused by ovarian function decline and hormone insufficiency, and is a high risk factor for cardiovascular diseases(CVD) and type II diabetes mellitus(T2DM). Erzhiwan(EZW),composed of Herba Ecliptae and Fructus Ligustri Lucidi, is a traditional Chinese herbal formula that has been used to treat menopausal syndrome for many years. We added Herba Epimedii, Radix Rehmanniae, and Fructus Corni into EZW, to prepare a new formula, termed Jiawei Erzhiwan(JE). The present study was designed to determine the anti-MMS effects of JE using ovariectomized(OVX) adult female rats that were treated with JE for 4 weeks, and β-tc-6 cells and INS cells were used to detected the protect effectiveness of JE. Our results showed JE could increase insulin sensitivity and ameliorated hyperlipidemia. Metabolomics analysis showed that the serum levels of branched and aromatic amino acids were down-regulated in serum by JE administration. Moreover, JE enhanced the function of islet βcells INS-1 and β-tc-6, through increasing the glucose stimulated insulin secretion(GSIS), which was abolished by estrogen receptor(ER)antagonist, indicating that JE functions were mediated by ER signaling. Additionally, JE did not induce tumorigenesis in rat mammary tissue or promoted proliferation of MCF-7 and Hela cells. In conclusion, our work demonstrated that JE ameliorated OVX-induced glucose and lipid metabolism disorder through activating estrogen receptor pathway and promoting GSIS in islet β cells, thus indicating that JE could be a safe and effective medication for MMS therapy.展开更多
目的:观察中药二至丸对老年痴呆(alzhe im er d isease AD)模型小鼠脑组织Na+-K+-ATPase、Ca2+-ATPase活性及学习记忆的影响。方法:颈背部皮下注射D-半乳糖建立亚急性老年痴呆模型,测定脑组织中Na+-K+-ATPase、Ca2+-ATPase活性、采用跳...目的:观察中药二至丸对老年痴呆(alzhe im er d isease AD)模型小鼠脑组织Na+-K+-ATPase、Ca2+-ATPase活性及学习记忆的影响。方法:颈背部皮下注射D-半乳糖建立亚急性老年痴呆模型,测定脑组织中Na+-K+-ATPase、Ca2+-ATPase活性、采用跳台法测定小鼠的学习记忆能力。结果:中药二至丸能明显提高Na+-K+-ATPase、Ca2+-ATPase活性、缩短小鼠学习潜伏期,延长记忆潜伏期、减少错误次数。结论:中药二至丸能改善老年痴呆模型小鼠的学习记忆能力,提高ATP酶的活性,维持脑组织的正常功能。展开更多
基金supported by the Funds for Creative Research Groups of China,National Natural Science Foundation of China(No.81421005)the New Century Excellent Talents in University(NCET-12-0976)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Fundamental Research Funds for the Central Universities(No.ZD2014YW0030)
文摘Menopausal metabolic syndrome(MMS) is a series of syndrome caused by ovarian function decline and hormone insufficiency, and is a high risk factor for cardiovascular diseases(CVD) and type II diabetes mellitus(T2DM). Erzhiwan(EZW),composed of Herba Ecliptae and Fructus Ligustri Lucidi, is a traditional Chinese herbal formula that has been used to treat menopausal syndrome for many years. We added Herba Epimedii, Radix Rehmanniae, and Fructus Corni into EZW, to prepare a new formula, termed Jiawei Erzhiwan(JE). The present study was designed to determine the anti-MMS effects of JE using ovariectomized(OVX) adult female rats that were treated with JE for 4 weeks, and β-tc-6 cells and INS cells were used to detected the protect effectiveness of JE. Our results showed JE could increase insulin sensitivity and ameliorated hyperlipidemia. Metabolomics analysis showed that the serum levels of branched and aromatic amino acids were down-regulated in serum by JE administration. Moreover, JE enhanced the function of islet βcells INS-1 and β-tc-6, through increasing the glucose stimulated insulin secretion(GSIS), which was abolished by estrogen receptor(ER)antagonist, indicating that JE functions were mediated by ER signaling. Additionally, JE did not induce tumorigenesis in rat mammary tissue or promoted proliferation of MCF-7 and Hela cells. In conclusion, our work demonstrated that JE ameliorated OVX-induced glucose and lipid metabolism disorder through activating estrogen receptor pathway and promoting GSIS in islet β cells, thus indicating that JE could be a safe and effective medication for MMS therapy.
文摘目的:观察中药二至丸对老年痴呆(alzhe im er d isease AD)模型小鼠脑组织Na+-K+-ATPase、Ca2+-ATPase活性及学习记忆的影响。方法:颈背部皮下注射D-半乳糖建立亚急性老年痴呆模型,测定脑组织中Na+-K+-ATPase、Ca2+-ATPase活性、采用跳台法测定小鼠的学习记忆能力。结果:中药二至丸能明显提高Na+-K+-ATPase、Ca2+-ATPase活性、缩短小鼠学习潜伏期,延长记忆潜伏期、减少错误次数。结论:中药二至丸能改善老年痴呆模型小鼠的学习记忆能力,提高ATP酶的活性,维持脑组织的正常功能。
