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Comparison of Self-Perception of Esthetics and Orthodontic Treatment Needs among Dental and Non-Dental Students with Assessment by Orthodontist
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作者 Yousef Al-Thomali 《Open Journal of Stomatology》 CAS 2023年第2期89-96,共8页
Objectives: The present study was conducted to compare the self-perception of esthetics and wish for seeking orthodontic treatment among dental and non-dental students. The realistic orthodontic treatment needs were c... Objectives: The present study was conducted to compare the self-perception of esthetics and wish for seeking orthodontic treatment among dental and non-dental students. The realistic orthodontic treatment needs were confirmed by expert orthodontist. Methods: A cross-sectional study was conducted among 255 male students (Dental—75, Medical—93 and Pharmacy—87 students). The questionnaire included 20 items covering dental esthetics self-perception (10 questions), self-confidence and psychological impact (5 questions), and the need for seeking orthodontic treatment (5 questions) on a five-point Likert scale. The realistic orthodontic treatment needs were confirmed by photographs using aesthetic component of IOTN and clinical examination for the participants and analysis of jaw models using Dental aesthetic index (DAI). Kruskal-Wallis ANOVA was used to check the significant difference between groups. Results: The overall average esthetic self-perception score for total sample was 3.83. The overall average psychological impact score for the whole sample was 2.67. The total percentage of participants who either agree or strongly agree with the question related to their perception of need for seeking orthodontic treatment was 70%. According to the assessment of orthodontic needs using AC-IOTN, 19.8% required definitive treatment. According to the assessment of orthodontic treatment needs based on DAI, 20.3% and 9.9% needed highly desirable (DAI 31 - 35) and definite need of treatment (DAI ≥ 36) respectively. Conclusion: It can be concluded that there was no difference between the esthetic perception of dental and non-dental students. The self-perception for seeking orthodontic treatment was over-reported by participants compared to real requirement as assessed by orthodontic specialist. Dental esthetics has no psychological impact on academic performance and self-confidence as perceived by study participants. 展开更多
关键词 SELF-PERCEPTION Orthodontic Treatment Needs Orthodontist Opinion esthetics
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Satisfaction of Adult Patients about Their Smile Aesthetics Compared to Dental Professionals Observation
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作者 Basil Yousef Alamassi Mohammad Saleh Al Onazi Ahmad Abdullah Al Zoman 《Open Journal of Stomatology》 2016年第11期236-244,共10页
Objective: The aim of the present study was to evaluate personal satisfaction of adult dental patients about their smiles aesthetics in comparison to dental professionals view. Method: One hundred twenty adult patient... Objective: The aim of the present study was to evaluate personal satisfaction of adult dental patients about their smiles aesthetics in comparison to dental professionals view. Method: One hundred twenty adult patients (76 male and 44 female) visiting the screening clinics of Riyadh colleges of dentistry and Pharmacy were asked to evaluate their smiles by completing a questionnaire containing five questions concerning five aesthetic elements which are: Overall smile;color;shape;alignment of their teeth;and appearance of their gum during smiling. They were asked to rate each of the five categories in Visual Analog Scale (VAS) graded from 0 to 10. A smiling photograph was taken for each patient to be evaluated by two restorative specialists using the same scale. Patients and dental professional mean evaluation scores were statistically analyzed for any significant differences. Result: The average age of the patients was 39.1 years. The mean patients’ satisfaction with their own smiles was [6.47] on the VAS which was statistically higher than the average of dentists’ evaluation scores [4.95] (p Conclusion: Patients’ satisfaction about their dental aesthetics was statistically higher than the dental professional assessments. Dental professionals appear to be more critical in their evaluation of patients smile aesthetics. Age showed no significant relation with the level of satisfaction while gender and reason of dental visit showed a significant relation with patients smile satisfaction. 展开更多
关键词 dental AESTHETIC SATISFACTION SMILE Perception
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Self-assembly of differentiated dental pulp stem cells facilitates spheroid human dental organoid formation and prevascularization
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作者 Fei Liu Jing Xiao +4 位作者 Lei-Hui Chen Yu-Yue Pan Jun-Zhang Tian Zhi-Ren Zhang Xiao-Chun Bai 《World Journal of Stem Cells》 SCIE 2024年第3期287-304,共18页
BACKGROUND The self-assembly of solid organs from stem cells has the potential to greatly expand the applicability of regenerative medicine.Stem cells can self-organise into microsized organ units,partially modelling ... BACKGROUND The self-assembly of solid organs from stem cells has the potential to greatly expand the applicability of regenerative medicine.Stem cells can self-organise into microsized organ units,partially modelling tissue function and regeneration.Dental pulp organoids have been used to recapitulate the processes of tooth development and related diseases.However,the lack of vasculature limits the utility of dental pulp organoids.AIM To improve survival and aid in recovery after stem cell transplantation,we demonstrated the three-dimensional(3D)self-assembly of adult stem cell-human dental pulp stem cells(hDPSCs)and endothelial cells(ECs)into a novel type of spheroid-shaped dental pulp organoid in vitro under hypoxia and conditioned medium(CM).METHODS During culture,primary hDPSCs were induced to differentiate into ECs by exposing them to a hypoxic environment and CM.The hypoxic pretreated hDPSCs were then mixed with ECs at specific ratios and conditioned in a 3D environment to produce prevascularized dental pulp organoids.The biological characteristics of the organoids were analysed,and the regulatory pathways associated with angiogenesis were studied.RESULTS The combination of these two agents resulted in prevascularized human dental pulp organoids(Vorganoids)that more closely resembled dental pulp tissue in terms of morphology and function.Single-cell RNA sequencing of dental pulp tissue and RNA sequencing of Vorganoids were integrated to analyse key regulatory pathways associated with angiogenesis.The biomarkers forkhead box protein O1 and fibroblast growth factor 2 were identified to be involved in the regulation of Vorganoids.CONCLUSION In this innovative study,we effectively established an in vitro model of Vorganoids and used it to elucidate new mechanisms of angiogenesis during regeneration,facilitating the development of clinical treatment strategies. 展开更多
关键词 Human dental pulp stem cells Prevascularized organoids Integrated analyses ANGIOGENESIS Forkhead box protein O1
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Prevalence of Dental Anomalies among Patients Who Report to the Komfo Anokye Teaching Hospital’s Orthodontic Clinic
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作者 Ama Agyeibea Amuasi Daniel K. Sabbah +1 位作者 Alexander Oti-Achempong Rahman N. Mamah 《Open Journal of Stomatology》 2024年第2期103-117,共15页
BACKGROUND: Dental anomalies are variations from the established well-known general anatomy and morphology of the tooth as a result of disturbances during tooth formation. They can be developmental, congenital, or acq... BACKGROUND: Dental anomalies are variations from the established well-known general anatomy and morphology of the tooth as a result of disturbances during tooth formation. They can be developmental, congenital, or acquired and may be localized to a single tooth or involve systemic conditions. AIM: To evaluate the prevalence of dental anomalies in patients who report to the Komfo Anokye Teaching Hospital (KATH) dental clinics. METHOD: A descriptive cross-sectional design was used with a sample size of 92 patients aged 18 or older, obtained through convenience sampling. Data analysis was performed using SPSS version 26.0. RESULTS: The study included 92 patients aged 18 to 72 years, with 47.8% males and 52.2% females. Dental anomalies were observed in 51.1% of participants, with a higher prevalence in females (55.3%). The most common anomalies were diastema (48.3%), impacted teeth (22.0%), dilaceration (11.9%), and peg-shaped lateral teeth (6.8%). CONCLUSION: This study highlights the importance of conducting thorough dental examinations to identify and address dental anomalies, which may have implications for treatment. Early detection and correction of these anomalies are crucial to prevent future complications. 展开更多
关键词 dental Anomalies PREVALENCE Impacted Teeth Dilaceration
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Associated Factors of Dental Caries among Intellectually Disabled Persons in Specialized Centers of Ouagadougou
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作者 Jocelyne Valerie Gare Filwende Dan Kientega +3 位作者 Ouedraogo Carole Nathaie Tenin Ouattara Seydou Kabore Wendpoulomde Aime Désire Nicolas Meda 《Open Journal of Epidemiology》 2024年第2期297-311,共15页
Introduction: Several studies have reported a high prevalence of dental caries among the people with intellectual disabilities. In order to identify the sociodemographic, clinical and behavioral factors associated wit... Introduction: Several studies have reported a high prevalence of dental caries among the people with intellectual disabilities. In order to identify the sociodemographic, clinical and behavioral factors associated with it, we conducted a study in specialized centers welcoming subjects with intellectual disabilities in Ouagadougou (Burkina Faso). Objectives: The objective of this study was to identify the factors associated with it among intellectually disabled in the specialized centers of Ouagadougou. Methods: The study was cross-sectional analytical. The survey took place between November 2020 and January 2021 among people with intellectual disabilities in reception centers receiving a grant from the Ministry of Women, National Solidarity, Family and Humanitarian Action. Data collection was done with a questionnaire adapted from the World Health Organization (WHO) and dental caries was recorded according to WHO criteria in decayed, missing or filled teeth generating a DMF index. Logistic regression was used to identify factors associated with the presence of dental caries. Results: A total of 193 participants were included in the study with an average age of 12.4 years ± 7.9. The overall prevalence of caries measured with the DMF index was 58.6% 95% CI [51.2 - 65.8]. It was significantly associated with female gender (OR = 4.2;95% CI [1.4 - 12.5], p = 0,01), the clinical form of mental illness (epilepsy OR = 3.8 95%;CI [1.2 - 12.7], p = 0,02, trisomy OR = 5.0;95% CI [1.1 - 22.9], p = 0,03, motor autonomy OR = 0.2 95%;CI [0.1 - 0.7], p = 0,01) and at use of toothpaste OR = 9.33;95% CI [1.05 - 82.7], p = 0,04. Conclusion: Dental caries remains a very present pathology in most people living with an intellectual disability. Also, it is necessary to put in place prevention strategies to reduce its prevalence and improve access to oral care for these people. 展开更多
关键词 dental Caries Mental Disability Intellectual Disability Associated Factors OUAGADOUGOU
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An overview of autophagy in the differentiation of dental stem cells
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作者 XITONG ZHAO TIANJUAN JU +3 位作者 XINWEI LI CHANGFENG LIU LULU WANG LI-AN WU 《BIOCELL》 SCIE 2024年第1期47-64,共18页
Dental stem cells(DSCs)have attracted significant interest as autologous stem cells since they are easily accessible and give a minimal immune response.These properties and their ability to both maintain self-renewal ... Dental stem cells(DSCs)have attracted significant interest as autologous stem cells since they are easily accessible and give a minimal immune response.These properties and their ability to both maintain self-renewal and undergo multi-lineage differentiation establish them as key players in regenerative medicine.While many regulatory factors determine the differentiation trajectory of DSCs,prior research has predominantly been based on genetic,epigenetic,and molecular aspects.Recent evidence suggests that DSC differentiation can also be influenced by autophagy,a highly conserved cellular process responsible for maintaining cellular and tissue homeostasis under various stress conditions.This comprehensive review endeavors to elucidate the intricate regulatory mechanism and relationship between autophagy and DSC differentiation.To achieve this goal,we dissect the intricacies of autophagy and its mechanisms.Subsequently,we elucidate its pivotal roles in impacting DSC differentiation,including osteo/odontogenic,neurogenic,and angiogenic trajectories.Furthermore,we reveal the regulatory factors that govern autophagy in DSC lineage commitment,including scaffold materials,pharmaceutical cues,and the extrinsic milieu.The implications of this review are far-reaching,underpinning the potential to wield autophagy as a regulatory tool to expedite DSC-directed differentiation and thereby promote the application of DSCs within the realm of regenerative medicine. 展开更多
关键词 dental stem cells Cell differentiation AUTOPHAGY MITOPHAGY Autophagy regulation
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Conditioned medium from human dental pulp stem cells treats spinal cord injury by inhibiting microglial pyroptosis
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作者 Tao Liu Ziqian Ma +8 位作者 Liang Liu Yilun Pei Qichao Wu Songjie Xu Yadong Liu Nan Ding Yun Guan Yan Zhang Xueming Chen 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第5期1105-1111,共7页
Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury.However,whether the human dental pulp stem cell secretome can contribute to functional recovery... Human dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury.However,whether the human dental pulp stem cell secretome can contribute to functional recovery after spinal cord injury remains unclear.In the present study,we established a rat model of spinal cord injury based on impact injury from a dropped weight and then intraperitoneally injected the rats with conditioned medium from human dental pulp stem cells.We found that the conditioned medium effectively promoted the recovery of sensory and motor functions in rats with spinal cord injury,decreased expression of the microglial pyroptosis markers NLRP3,GSDMD,caspase-1,and interleukin-1β,promoted axonal and myelin regeneration,and inhibited the formation of glial scars.In addition,in a lipopolysaccharide-induced BV2 microglia model,conditioned medium from human dental pulp stem cells protected cells from pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway.These results indicate that conditioned medium from human dental pulp stem cells can reduce microglial pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1βpathway,thereby promoting the recovery of neurological function after spinal cord injury.Therefore,conditioned medium from human dental pulp stem cells may become an alternative therapy for spinal cord injury. 展开更多
关键词 BV2 conditioned medium dental pulp stem cells GSDMD MICROGLIA NEUROINFLAMMATION NLRP3 PYROPTOSIS spinal cord injury
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Hepatocyte growth factor enhances the ability of dental pulp stem cells to ameliorate atherosclerosis in apolipoprotein E-knockout mice
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作者 Han Duan Ning Tao +8 位作者 Lin Lv Kai-Xin Yan Yong-Gang You Zhuang Mao Chang-Yao Wang Xue Li Jia-Yan Jin Chu-Tse Wu Hua Wang 《World Journal of Stem Cells》 SCIE 2024年第5期575-590,共16页
BACKGROUND Atherosclerosis(AS),a chronic inflammatory disease of blood vessels,is a major contributor to cardiovascular disease.Dental pulp stem cells(DPSCs)are capable of exerting immunomodulatory and anti-inflammato... BACKGROUND Atherosclerosis(AS),a chronic inflammatory disease of blood vessels,is a major contributor to cardiovascular disease.Dental pulp stem cells(DPSCs)are capable of exerting immunomodulatory and anti-inflammatory effects by secreting cytokines and exosomes and are widely used to treat autoimmune and inflam-mation-related diseases.Hepatocyte growth factor(HGF)is a pleiotropic cytokine that plays a key role in many inflammatory and autoimmune diseases.AIM To modify DPSCs with HGF(DPSC-HGF)and evaluate the therapeutic effect of DPSC-HGF on AS using an apolipoprotein E-knockout(ApoE-/-)mouse model and an in vitro cellular model.METHODS ApoE-/-mice were fed with a high-fat diet(HFD)for 12 wk and injected with DPSC-HGF or Ad-Null modified DPSCs(DPSC-Null)through tail vein at weeks 4,7,and 11,respectively,and the therapeutic efficacy and mechanisms were analyzed by histopathology,flow cytometry,lipid and glucose measurements,real-time reverse transcription polymerase chain reaction(RT-PCR),and enzyme-linked immunosorbent assay at the different time points of the experiment.An in vitro inflammatory cell model was established by using RAW264.7 cells and human aortic endothelial cells(HAOECs),and indirect co-cultured with supernatant of DPSC-Null(DPSC-Null-CM)or DPSC-HGF-CM,and the effect and mechanisms were analyzed by flow cytometry,RT-PCR and western blot.Nuclear factor-κB(NF-κB)activators and inhibitors were also used to validate the related signaling pathways.RESULTS DPSC-Null and DPSC-HGF treatments decreased the area of atherosclerotic plaques and reduced the expression of inflammatory factors,and the percentage of macrophages in the aorta,and DPSC-HGF treatment had more pronounced effects.DPSCs treatment had no effect on serum lipoprotein levels.The FACS results showed that DPSCs treatment reduced the percentages of monocytes,neutrophils,and M1 macrophages in the peripheral blood and spleen.DPSC-Null-CM and DPSC-HGF-CM reduced adhesion molecule expression in tumor necrosis factor-αstimulated HAOECs and regulated M1 polarization and inflammatory factor expression in lipopolysaccharide-induced RAW264.7 cells by inhibiting the NF-κB signaling pathway.CONCLUSION This study suggested that DPSC-HGF could more effectively ameliorate AS in ApoE-/-mice on a HFD,and could be of greater value in stem cell-based treatments for AS. 展开更多
关键词 ATHEROSCLEROSIS Apolipoprotein E-knockout mice Cell therapy dental pulp stem cells Hepatocyte growth factor
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Human dental pulp stem/stromal cells in clinical practice
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作者 Mohammed E Grawish 《World Journal of Stem Cells》 SCIE 2024年第2期54-57,共4页
Dental pulp stem/stromal cells(DPSCs)are fibroblast-like,neural crest-derived,and multipotent cells that can differentiate into several lineages.They are relatively easy to isolate from healthy and inflamed pulps,with... Dental pulp stem/stromal cells(DPSCs)are fibroblast-like,neural crest-derived,and multipotent cells that can differentiate into several lineages.They are relatively easy to isolate from healthy and inflamed pulps,with little ethical concerns and can be successfully cryopreserved and thawed.The therapeutic effects of DPSCs derived from animal or human sources have been extensively studied through in-vitro and in-vivo animal experiments and the findings indicated that DPSCs are effective not only for dental diseases but also for systemic diseases.Understanding that translational research is a critical step through which the fundamental scientific discoveries could be translated into applicable diagnostics and therapeutics that directly benefit humans,several clinical studies were carried out to generate evidence for the efficacy and safety of autogenous or allogeneic human DPSCs(hDPSCs)as a treatment modality for use in cell-based therapy,regenerative medicine/dentistry and tissue engineering.In clinical medicine,hDPSCs were effective for treating acute ischemic stroke and human exfoliated deciduous teeth-conditioned medium(SHED-CM)repaired vascular damage of the corpus cavernous,which is the main cause of erectile dysfunction.Whereas in clinical dentistry,autologous SHED was able to rege-nerate necrotic dental pulp after implantation into injured teeth,and micrografts enriched with autologous hDPSCs and collagen sponge were considered a treatment option for human intrabony defects.In contrast,hDPSCs did not add a significant regenerative effect when they were used for the treatment of post-extraction sockets.Large-scale clinical studies across diverse populations are still lacking to provide robust evidence on the safety and efficacy of hDPSCs as a new treatment option for various human diseases including dental-related problems. 展开更多
关键词 dental pulp stem/stromal cells Human clinical studies Regenerative medicine Regenerative dentistry Cell-based therapy
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Hepatitis B Vaccination in Medical and Dental Students: A Cross-Sectional Study
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作者 Md. Usama Nasr Md. Kamran Saleem Abdullah Saeed 《Open Journal of Internal Medicine》 2024年第1期122-130,共9页
Background: Medical and dental students are at risk of Hepatitis B Virus (HBV) infection. The study aimed to assess the vaccination status against Hepatitis B Virus of students in clinical and non-clinical years of a ... Background: Medical and dental students are at risk of Hepatitis B Virus (HBV) infection. The study aimed to assess the vaccination status against Hepatitis B Virus of students in clinical and non-clinical years of a private medical and dental college, and their knowledge, attitude, and awareness about the subject. Methods: A cross-sectional study was conducted using a pretested, self-administered questionnaire among 203 medical and dental students of CMH Lahore Medical College and Institute of Dentistry (CMH LMC & IOD) in Lahore, Pakistan. Participants were evaluated for their knowledge and vaccination status against Hepatitis B Virus. Students were considered to be fully vaccinated (recipients of 3 doses), partially vaccinated (recipients of 1 or 2 doses), and unvaccinated. Comparisons were made between students of clinical and non-clinical years. Data was entered and analysed using Statistical Package for the Social Sciences (SPSS) version 23. Results: Only 66% (n = 134) of the 203 participants had ever received a Hepatitis B Virus vaccine out of which a meagre 17.2% (n = 35) were fully vaccinated. No significant difference was found in vaccine uptake between students of clinical and non-clinical years (p-value = 0.181) despite significant differences seen in the knowledge of vaccination schedule (p-value = 0.001), the prevalence of needle-stick injuries (p-value = 0.001), and knowledge of protocols to be followed after a needle-stick injury (p-value = 0.001). Conclusion: Our study found that a large proportion of the student population is vulnerable to HBV infection. There is a need to create awareness regarding the subject to increase vaccine uptake. HBV vaccination should be offered to all currently enrolled students and be made mandatory at the time of admission in the future. 展开更多
关键词 Hepatitis B Vaccine Medical Students dental Students Hepatitis B Vaccination Needle-Stick Injuries
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Comparison of Oral Manifestations in Hospitalized COVID-19 Positive Patients and COVID-19 Negative Dental Outpatients. A Case Series Study and Literature Review
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作者 Flora Zervou-Valvi Emmanouil M. Valvis +5 位作者 Angeliki Giannopoulou Eleana Stoufi Charilaos Samaras Olga Spiropoulou Antonia Hadjicosta Smaragda Diamanti 《International Journal of Otolaryngology and Head & Neck Surgery》 2024年第3期225-250,共26页
Background: The Coronavirus disease 2019 (COVID-19) is a respiratory infectious disease, also named Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which can cause various systemic manifestations that po... Background: The Coronavirus disease 2019 (COVID-19) is a respiratory infectious disease, also named Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), which can cause various systemic manifestations that pose a threat to human life. Oral lesions in patients with COVID-19 may appear during or after the illness and may or may not be a consequence of the viral infection. Objective: In this case series we compare the oral manifestations in hospitalized COVID-19 positive patients and COVID-19 negative dental outpatients. Methods: 60 hospitalized COVID-19 patients and 41 control patients, were examined for oral signs and symptoms. The controls were dental patients who visited the hospital for dental care without complaining of any problems related to the oral cavity itself. Results: We have observed a strong association between certain clinical findings and COVID-19, including alterations in taste (ageusia, dysgeusia, and hypogeusia), anosmia, hairy tongue, tongue imprints, red tongue, erythematous candidiasis, pseudomembranous candidiasis, and exfoliative cheilitis. A trend but not statistically significant association at the level of 5% was also noted for colored tongue, linea alba, and pale mucosa. On the contrary, fissured tongue and oral mucosa pigmentation were more frequent in the controls, statistically significant at the level of 5%. Conclusion: COVID-19 has been found to impact the oral cavity, resulting in various oral lesions that can be attributed to either the direct action of the virus or the patient’s immune response. 展开更多
关键词 Case Series COVID-19 SARS-CoV-2 Oral Lesions/Manifestations Hospitalized Patients Control/dental Patients
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Dental Aesthetics: A Study Comparing Patients’ Own Opinions with Those of Dentists
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作者 Richard John Moon Brian James Millar 《Open Journal of Stomatology》 2017年第4期225-233,共9页
Objective: A beautiful smile is perceived as important but the components that contribute to the patient’s concept of a beautiful smile have not been fully investigated. Hence this study aimed to compare the views of... Objective: A beautiful smile is perceived as important but the components that contribute to the patient’s concept of a beautiful smile have not been fully investigated. Hence this study aimed to compare the views of patients on their own dental aesthetics with those of a group of dentists. It also assessed the patients’ willingness to undergo aesthetic treatment. Methods: Fifty patients, who ranged in age from 24 to 76 years, completed self-assessment questionnaires. Photographs were taken of these patients, which were subsequently assessed by six dentists using a questionnaire with visual analogue scale to assess each parameter. Results: Significant differences (p < 0.05) were found between the opinions of the dentists and the patients. Older patients were generally more satisfied with their smile than the dentists. Eighty-six percent of the patients were willing to undergo aesthetic treatment, although factors such as the complexity of treatment, time involved, discomfort and financial costs, deterred many. The cost of treatment was the main deterrent. The younger patients were least likely to be put off treatment. Conclusion: Patients’ views of their own smile differed from the dentists’ opinion. Those who were the least satisfied and were most likely to undergo aesthetic treatment were in the younger age groups. Satisfaction increased with age and older patients were less likely to seek the aesthetic treatment. 展开更多
关键词 dental Aesthetics SELF-ASSESSMENT Patient DENTIST
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Genetic modification of miR-34a enhances efficacy of transplanted human dental pulp stem cells after ischemic stroke 被引量:1
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作者 Jianfeng Wang Peibang He +7 位作者 Qi Tian Yu Luo Yan He Chengli Liu Pian Gong Yujia Guo Qingsong Ye Mingchang Li 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第9期2029-2036,共8页
Human dental pulp stem cells(hDPSCs) promote recovery after ischemic stro ke;however,the therapeutic efficacy is limited by the poor survival of transplanted cells.For in vitro expe riments in the present study,we use... Human dental pulp stem cells(hDPSCs) promote recovery after ischemic stro ke;however,the therapeutic efficacy is limited by the poor survival of transplanted cells.For in vitro expe riments in the present study,we used oxygen-glucose deprivation/reoxygenation in hDPSCs to mimic cell damage induced by ischemia/reperfusion.We found that miRNA-34a-5p(miR-34a) was elevated under oxygen-glucose deprivation/reoxygenation conditions in hDPSCs.Inhibition of miR-34a facilitated the prolife ration and antioxidant capacity and reduced the apoptosis of hDPSCs.Moreove r,dual-luciferase reporter gene assay showed WNT1and SIRT1 as the targets of miR-34a.In miR-34a knockdown cell lines,WNT1 suppression reduced cell prolife ration,and SIRT1 suppression decreased the antioxidant capacity.Togethe r,these results indicated that miR-34a regulates cell prolife ration and antioxidant stress via targeting WNT1 and SIRT1,respectively.For in vivo expe riments,we injected genetically modified hDPSCs(anti34a-hDPSCs) into the brains of mice.We found that anti34a-hDPSCs significantly inhibited apoptosis,reduced cerebral edema and cerebral infarct volume,and improved motor function in mice.This study provides new insights into the molecular mechanism of the cell prolife ration and antioxidant capacity of hDPSCs,and suggests a potential gene that can be targeted to improve the survival rate and efficacy of transplanted hDPSCs in brain after ischemic stroke. 展开更多
关键词 antioxidant capacity HO-1 human dental pulp stem cells ischemic stroke MIR-34A Nrf2 proliferation SIRT1 WNT1 β-catenin
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MSCT Dental技术推断10~20岁人群年龄的准确性研究
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作者 王蕾 秦大伟 +6 位作者 张伟 尚敏 马精晶 赵晓云 姚泽伟 秦豪杰 李凡 《中国中西医结合影像学杂志》 2023年第2期203-205,共3页
目的:探讨MSCT Dental技术在推断青少年年龄方面的准确性。方法:使用MSCT Dental技术测量88例10~20岁青少年牙齿的牙槽骨高度。利用SPSS 22.0软件构建回归模型,分析MSCT Dental技术推断年龄的准确性。结果:利用牙槽骨高度构建的模型均... 目的:探讨MSCT Dental技术在推断青少年年龄方面的准确性。方法:使用MSCT Dental技术测量88例10~20岁青少年牙齿的牙槽骨高度。利用SPSS 22.0软件构建回归模型,分析MSCT Dental技术推断年龄的准确性。结果:利用牙槽骨高度构建的模型均能进行年龄推断,但由上颌右侧第一前磨牙构建模型推断的年龄准确性最高,与实际年龄相比,差异无统计学意义(P>0.05),可用于推断年龄。结论:MSCT Dental技术用于推断青少年年龄时误差较小,多数可控制在1岁以内,实际工作中可以使用。 展开更多
关键词 虚拟法医人类学 dental技术 年龄 准确性 青少年 体层摄影术 X线计算机
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Clinical trials using dental stem cells:2022 update 被引量:2
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作者 Wen-Peng Song Lu-Yuan Jin +2 位作者 Meng-Di Zhu Hao Wang Deng-Sheng Xia 《World Journal of Stem Cells》 SCIE 2023年第3期31-51,共21页
For nearly 20 years,dental stem cells(DSCs)have been successfully isolated from mature/immature teeth and surrounding tissue,including dental pulp of permanent teeth and exfoliated deciduous teeth,periodontal ligament... For nearly 20 years,dental stem cells(DSCs)have been successfully isolated from mature/immature teeth and surrounding tissue,including dental pulp of permanent teeth and exfoliated deciduous teeth,periodontal ligaments,dental follicles,and gingival and apical papilla.They have several properties(such as self-renewal,multidirectional differentiation,and immunomodulation)and exhibit enormous potential for clinical applications.To date,many clinical articles and clinical trials using DSCs have reported the treatment of pulpitis,periapical lesions,periodontitis,cleft lip and palate,acute ischemic stroke,and so on,and DSC-based therapies obtained satisfactory effects in most clinical trials.In these studies,no adverse events were reported,which suggested the safety of DSC-based therapy.In this review,we outline the characteristics of DSCs and summ-arize clinical trials and their safety as DSC-based therapies.Meanwhile,we also present the current limitations and perspectives of DSC-based therapy(such as harvesting DSCs from inflamed tissue,applying DSC-conditioned medi-um/DSC-derived extracellular vesicles,and expanding-free strategies)to provide a theoretical basis for their clinical applications. 展开更多
关键词 dental stem cells Adult stem cells dental pulp Tissue regeneration
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Clinical application prospects and transformation value of dental follicle stem cells in oral and neurological diseases 被引量:1
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作者 Chao Yang Xin-Ya Du Wen Luo 《World Journal of Stem Cells》 SCIE 2023年第4期136-149,共14页
Since dental pulp stem cells(DPSCs)were first reported,six types of dental SCs(DSCs)have been isolated and identified.DSCs originating from the craniofacial neural crest exhibit dental-like tissue differentiation pote... Since dental pulp stem cells(DPSCs)were first reported,six types of dental SCs(DSCs)have been isolated and identified.DSCs originating from the craniofacial neural crest exhibit dental-like tissue differentiation potential and neuroectodermal features.As a member of DSCs,dental follicle SCs(DFSCs)are the only cell type obtained at the early developing stage of the tooth prior to eruption.Dental follicle tissue has the distinct advantage of large tissue volume compared with other dental tissues,which is a prerequisite for obtaining a sufficient number of cells to meet the needs of clinical applications.Furthermore,DFSCs exhibit a significantly higher cell proliferation rate,higher colony-formation capacity,and more primitive and better anti-inflammatory effects than other DSCs.In this respect,DFSCs have the potential to be of great clinical significance and translational value in oral and neurological diseases,with natural advantages based on their origin.Lastly,cryopreservation preserves the biological properties of DFSCs and enables them to be used as off-shelf products for clinical applications.This review summarizes and comments on the properties,application potential,and clinical transformation value of DFSCs,thereby inspiring novel perspectives in the future treatment of oral and neurological diseases. 展开更多
关键词 dental follicle stem cells Oral disease Neurological disease Tissue engineering REGENERATION IMMUNOREGULATION
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Actual Practical Attitude and Knowledge of Dental Implants among Senior Dental Students and General Dentists Graduated from Some Saudi and Non-Saudi Dental Schools
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作者 Mohammed M. A. Abdullah Al-Abdaly Sami Yahya Alamri +1 位作者 Ghadeer Mohammed Mohammed Al-Abdaly Ayed Abdulrahman Abdullah 《International Journal of Clinical Medicine》 CAS 2023年第1期1-19,共19页
Objective: To assess the actual practical attitude and knowledge of dental implants among senior dental students and general dentists graduated from some Saudi and Non-Saudi dental schools. Methods: A total of 300 sen... Objective: To assess the actual practical attitude and knowledge of dental implants among senior dental students and general dentists graduated from some Saudi and Non-Saudi dental schools. Methods: A total of 300 senior dental students and general dentists participated in the study. Hard copies of the self-designed, multiple-choice questionnaires were distributed to all participants. The questionnaire consisted of 31 questions in five parts. Data were collected and analyzed using Chi-square test and t-test, where p Results: There is a statistically significant relationship between the participants’ answers, and their dental schools. Participants’ general knowledge, training, and teaching of dental implants, as well as information about restorations retained for the dental implants, were higher among participants from Saudi dental schools than participants from non-Saudi dental schools, while the information about dental implants was higher among participants from non-Saudi dental schools than participants from Saudi dental schools. Conclusion: We conclude that the actual practical attitude and knowledge of dental implants among participants in the current study was insufficient. Therefore, dental implant education in the undergraduate curricula of dental schools surveyed should be updated to include teaching, laboratory training, and preclinical and clinical training. 展开更多
关键词 KNOWLEDGE dental Implants dental Schools Non-Saudi Practical Attitude SAUDI
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Managements of Special Health Care Needs Patients from Dental Assistant Point
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作者 Abdullah M. G. Al Enazi Dalal Khalaf Al Enazi +3 位作者 Haya Nasser Al Duaida Ashwag Bander Al Bakheet Mashael Muteb Al Ghobain Abeer Salem Al Harbi 《International Journal of Clinical Medicine》 CAS 2023年第2期59-64,共6页
The main role of this article is to describe dental assistants and the dental department’s role in the dental delivery system for Special health care needs (SCHN) by creating a structure to educate the dental assisti... The main role of this article is to describe dental assistants and the dental department’s role in the dental delivery system for Special health care needs (SCHN) by creating a structure to educate the dental assisting profession and project factors that have a significant impact on the dental assistant. The dental assisting management of SCHN patient’s profession including dental Assistance general responsibilities, regulation credentialing, and education. The SCHN patient’s management creates a challenge in all area of the dental department. Handling patient with a SCHN is underexplored throughout the dental staff especially when there is not enough theoretical foundation, education and training to deal with this category of patients. SCHN patients are defined as individuals who have abnormal behavioral impairments, mental conditions or/and chronic disease or abnormal laboratory results. Thus, the aim of this article is to guide all dental assistance staff in the best dental management choice for SCHN patients. All health care teams are part of patient care in most medical settings. A work environment supportive ensures positive outcomes for patient care. This article was considered as those categories of patients required more care and special way to deal with, moreover language, age, gender and education level of the patient are also considered significant parries. Not Many studies were found to support the care of SCHN patients in dentistry department. Taking experience from other area help to design a system to handle the SCHN even if it requires hiring a Dentist who is specialized in that filed. 展开更多
关键词 dental Management dental Assistant DENTIST Special Needs Special Health Care Need
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Daily Lifestyle Habits as Risk Factors for Plaque-Induced Gingivitis and Periodontitis Severity and Grading among Samples of Dental Students at King Khalid University
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作者 Mohammed M. A. Abdullah Al-Abdaly Amer Hassan Alasmari +3 位作者 Feras Ali Alsaeed Ali Abdullah Ali Alqhtani Fahad Hassan Alhifthi Yasir Saad Jaadan 《International Journal of Clinical Medicine》 CAS 2023年第2期65-78,共14页
Objective: To assess daily lifestyle habits as risk factors for plaque-induced gingivitis and periodontitis severity and grading among samples of dental students at King Khalid University. Material and Methods: This s... Objective: To assess daily lifestyle habits as risk factors for plaque-induced gingivitis and periodontitis severity and grading among samples of dental students at King Khalid University. Material and Methods: This study included 150 male participants. They were divided according to the levels into three equal groups: levels 5, 6, and 7 (group I), levels 8, 9, 10 (group II) and levels 11, 12, and interns (group III). This study consisted of questions related to age, gender, educational level, father’s education, mother’s education, home ownership, brushing teeth more than or twice a day, in addition to daily lifestyle habits that were daily smoking, daily use of sugary drinks, and daily use of sugary foods. Some clinical parameters of plaque-induced gingivitis and periodontitis and the percentage of radiographic bone loss were recorded. All data were collected and analyzed using Tukey’s test and Chi-square test. A P-value of less than 0.5 (p Results: One hundred fifty male dental students of different levels in the college of dentistry at King Khalid University participated in the study in three groups (I, II, and III). The mean age of group III is more than that of group II and group I, with highly statistically significant differences (p 30%) were more among the participants in group I than the participants in groups III and II. There was an increase in the percentage of affected participants with stages III and IV of clinical attachment loss (CAL), periodontal pocket depth (PPD), radiographic bone loss (%RBL), tooth mobility, as well as glycated hemoglobin (HbA1c) test values (>7%), and the number of cigarettes smoked per day (>10 cigs) in group III more than in group II and I. Conclusion: We conclude that the severity and grading of plaque-induced gingivitis were higher among the participants in group I, while the severity and grading of periodontitis were higher among the participants in group III due to the different impact of their daily lifestyle habits. 展开更多
关键词 Daily Lifestyle dental Students King Khalid University PERIODONTITIS Plaque-Induced Gingivitis
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Oral Health Management of Three Head and Neck Cancer Patients Performed by Dental Hygienists
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作者 Aiko Hoshiai Meiko Oki +3 位作者 Taiji Hoshiai Toshiaki Iida Yuka I. Sumita Shigeru Watanabe 《Health》 2023年第12期1402-1414,共13页
Introduction: Oral health management is often difficult in patients after surgical tumor resection and/or radiotherapy due to defects, bulky flaps, limitation of tongue movement, and trismus. In addition, patients who... Introduction: Oral health management is often difficult in patients after surgical tumor resection and/or radiotherapy due to defects, bulky flaps, limitation of tongue movement, and trismus. In addition, patients who have undergone radiotherapy have serious disabilities such as oral mucositis, mucosal weakening, soft tissue fibrosis, salivary gland disorder, and osteoradionecrosis. Dental hygienists must understand the characteristics of patients after surgical tumor resection and/or radiotherapy. In this report, the oral health management of three patients after maxillectomy, mandibulectomy, and radiotherapy was shown. Case Description: Case 1: A 53-year-old male patient visited our clinic after chemotherapy, radiotherapy, and partial maxillectomy for cancer of the right maxillary gingiva. Case 2: A 65-year-old male patient visited our clinic after radiotherapy for a right-sided tongue cancer, partial mandibulectomy of the right tongue to the oropharynx, a right-sided segmental mandibulectomy and a speech aid and a mandibular prosthesis were provided. Case 3: A 36-year-old female visited our clinic for radiotherapy for left-sided tongue cancer. Dental hygienists provided oral health management to the patients. In oral health management, it was suggested that intervention before cancer treatment, oral hygiene instructions tailored to patients’ symptoms, and frequent professional care are important. Conclusion: Oral health management by dental hygienists is extremely important for patients undergoing maxillofacial cancer treatment to maintain their quality of life in the long term. 展开更多
关键词 Head and Neck Cancer dental Hygienist Oral Health Management MAXILLECTOMY Mandibulectomy Interstitial Radiotherapy
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