死亡相关蛋白(Thanatos-associated proteins,THAP)家族的许多成员已被证实与细胞增殖、凋亡和癌症密切相关,而THAP8的功能尚不清楚。为了探究THAP8在癌症中的表达,对TCGA和Oncomine数据库进行了分析,发现THAP8 m RNA在肺癌组织的表达...死亡相关蛋白(Thanatos-associated proteins,THAP)家族的许多成员已被证实与细胞增殖、凋亡和癌症密切相关,而THAP8的功能尚不清楚。为了探究THAP8在癌症中的表达,对TCGA和Oncomine数据库进行了分析,发现THAP8 m RNA在肺癌组织的表达显著高于癌旁正常组织;接着采用荧光定量PCR技术分析了22对肺癌和癌旁正常组织中THAP8的表达差异,结果与数据库分析一致。为了探讨THAP8在肺癌组织中高表达的原因,用JASPAR软件预测THAP8启动子上的转录因子结合位点,发现THAP8基因启动子区含有4个缺氧诱导元件。荧光素酶活性分析实验证明,缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)可以结合到THAP8启动子上的缺氧诱导元件并促进THAP8基因的转录。在CoCl_(2)诱导的缺氧模型中,随着CoCl_(2)浓度的增加,HIF-1α和THAP8蛋白的表达水平升高。此外,在Beas-2B细胞中,过表达HIF-1α可以促进THAP8的表达。为了进一步研究THAP8在肺癌细胞中的功能,用慢病毒介导的shRNA敲低THAP8基因,分析THAP8基因沉默对肺癌细胞生物学行为的影响。结果表明:在H460和H1437肺癌细胞中,敲低THAP8基因可显著降低细胞的活力、克隆形成和侵袭能力。这些结果说明:THAP8可以受缺氧诱导,并促进肺癌细胞的活力、克隆形成和侵袭,是一个新的癌基因。展开更多
Set in a highly advanced society, Kazuo Ishiguro's Never Let Me Go tells a tragic story of a group of clones who are created to donate their organs for the purpose of saving homo sapiens' lives. This thesis ai...Set in a highly advanced society, Kazuo Ishiguro's Never Let Me Go tells a tragic story of a group of clones who are created to donate their organs for the purpose of saving homo sapiens' lives. This thesis aims to explore homo sapiens' living condition under modern civilization revealed in Kazuo Ishiguro's Never Let Me Go based on Marcuse's exploration of Western civilization. By creating a future world where technological rationality is at its apex, Kazuo Ishiguro shows us that people are still haunted by mortality and no happiness is guaranteed under that kind of civilization. Then by showing how those clones face their mortality under modern civilization, Kazuo Ishiguro pushes us to reflect on how human beings should live their lives in this modern world.展开更多
文摘死亡相关蛋白(Thanatos-associated proteins,THAP)家族的许多成员已被证实与细胞增殖、凋亡和癌症密切相关,而THAP8的功能尚不清楚。为了探究THAP8在癌症中的表达,对TCGA和Oncomine数据库进行了分析,发现THAP8 m RNA在肺癌组织的表达显著高于癌旁正常组织;接着采用荧光定量PCR技术分析了22对肺癌和癌旁正常组织中THAP8的表达差异,结果与数据库分析一致。为了探讨THAP8在肺癌组织中高表达的原因,用JASPAR软件预测THAP8启动子上的转录因子结合位点,发现THAP8基因启动子区含有4个缺氧诱导元件。荧光素酶活性分析实验证明,缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)可以结合到THAP8启动子上的缺氧诱导元件并促进THAP8基因的转录。在CoCl_(2)诱导的缺氧模型中,随着CoCl_(2)浓度的增加,HIF-1α和THAP8蛋白的表达水平升高。此外,在Beas-2B细胞中,过表达HIF-1α可以促进THAP8的表达。为了进一步研究THAP8在肺癌细胞中的功能,用慢病毒介导的shRNA敲低THAP8基因,分析THAP8基因沉默对肺癌细胞生物学行为的影响。结果表明:在H460和H1437肺癌细胞中,敲低THAP8基因可显著降低细胞的活力、克隆形成和侵袭能力。这些结果说明:THAP8可以受缺氧诱导,并促进肺癌细胞的活力、克隆形成和侵袭,是一个新的癌基因。
文摘Set in a highly advanced society, Kazuo Ishiguro's Never Let Me Go tells a tragic story of a group of clones who are created to donate their organs for the purpose of saving homo sapiens' lives. This thesis aims to explore homo sapiens' living condition under modern civilization revealed in Kazuo Ishiguro's Never Let Me Go based on Marcuse's exploration of Western civilization. By creating a future world where technological rationality is at its apex, Kazuo Ishiguro shows us that people are still haunted by mortality and no happiness is guaranteed under that kind of civilization. Then by showing how those clones face their mortality under modern civilization, Kazuo Ishiguro pushes us to reflect on how human beings should live their lives in this modern world.
