1 Introduction Ethylene, one of the simplest organic molecules with biological activity, is a planthormone that controls many processes of plant growth and development. Its synthesisis strictly regulated in plants. It...1 Introduction Ethylene, one of the simplest organic molecules with biological activity, is a planthormone that controls many processes of plant growth and development. Its synthesisis strictly regulated in plants. It is induced during some specific developmental stages,such as fruit ripening, seed germination and tissue senescence and abscission.展开更多
目的探讨蚯蚓纤溶酶(earthworm fibrinolytic enzyme,EFE)对裸鼠人肝癌细胞移植瘤生长及CD44v6表达的影响。方法建立肝癌SMMC-7721细胞裸鼠移植瘤模型,随机分为生理盐水组、EFE组、5-Fu组及联合用药组,计算抑瘤率及两药相互作用系数(coe...目的探讨蚯蚓纤溶酶(earthworm fibrinolytic enzyme,EFE)对裸鼠人肝癌细胞移植瘤生长及CD44v6表达的影响。方法建立肝癌SMMC-7721细胞裸鼠移植瘤模型,随机分为生理盐水组、EFE组、5-Fu组及联合用药组,计算抑瘤率及两药相互作用系数(coefficient of drug in interaction,CDI),并行血常规检测评价EFE的副反应。同时采用免疫组化SP染色法、RT-PCR及Western blot方法观察EFE组及生理盐水组瘤组织中CD44v6的表达情况。结果与生理盐水组相比,EFE组和5-Fu组抑瘤率分别为20.53%和23.07%;联合用药组抑瘤率为54.20%,且CDI<1。血常规检测提示EFE对造血系统无明显副反应。免疫组化和Western blot结果显示,EFE组CD44v6蛋白的表达水平较生理盐水组分别下调47.16%、28.37%(P<0.05)。RT-PCR结果表明,EFE组CD44v6 mRNA的表达水平亦较生理盐水组低,下调16.44%(P<0.05)。结论蚯蚓纤溶酶对裸鼠人肝癌移植瘤的生长具有一定的抑制作用,与5-Fu存在一定的协同效应,并可以抑制黏附分子CD44v6的表达。展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘1 Introduction Ethylene, one of the simplest organic molecules with biological activity, is a planthormone that controls many processes of plant growth and development. Its synthesisis strictly regulated in plants. It is induced during some specific developmental stages,such as fruit ripening, seed germination and tissue senescence and abscission.
文摘目的探讨蚯蚓纤溶酶(earthworm fibrinolytic enzyme,EFE)对裸鼠人肝癌细胞移植瘤生长及CD44v6表达的影响。方法建立肝癌SMMC-7721细胞裸鼠移植瘤模型,随机分为生理盐水组、EFE组、5-Fu组及联合用药组,计算抑瘤率及两药相互作用系数(coefficient of drug in interaction,CDI),并行血常规检测评价EFE的副反应。同时采用免疫组化SP染色法、RT-PCR及Western blot方法观察EFE组及生理盐水组瘤组织中CD44v6的表达情况。结果与生理盐水组相比,EFE组和5-Fu组抑瘤率分别为20.53%和23.07%;联合用药组抑瘤率为54.20%,且CDI<1。血常规检测提示EFE对造血系统无明显副反应。免疫组化和Western blot结果显示,EFE组CD44v6蛋白的表达水平较生理盐水组分别下调47.16%、28.37%(P<0.05)。RT-PCR结果表明,EFE组CD44v6 mRNA的表达水平亦较生理盐水组低,下调16.44%(P<0.05)。结论蚯蚓纤溶酶对裸鼠人肝癌移植瘤的生长具有一定的抑制作用,与5-Fu存在一定的协同效应,并可以抑制黏附分子CD44v6的表达。