This paper deals with the photosynthetic properties of Eucalyptue camphoia. E. camaldulenaia. E. gunni-i. E. viminalia. E. giandia. E. dunii and E. maidenii. including the daily bariations of photosynthetic and respir...This paper deals with the photosynthetic properties of Eucalyptue camphoia. E. camaldulenaia. E. gunni-i. E. viminalia. E. giandia. E. dunii and E. maidenii. including the daily bariations of photosynthetic and respiratory rates, the comparison of the photosynthetic rates of various Eucalyriua spp. measured,the relationship between photo-synthetic rate and chlorophyll content, as well as the light saturation point.展开更多
The blue gum chalcid, Leptocybe invasa Fisher & La Salle, invaded China in 2007 and has subsequently caused substantial damage to eucalyptus trees. In the current paper, we investigated the susceptibility of 10 Eucal...The blue gum chalcid, Leptocybe invasa Fisher & La Salle, invaded China in 2007 and has subsequently caused substantial damage to eucalyptus trees. In the current paper, we investigated the susceptibility of 10 Eucalyptus spp. and Eucahetus dunnii to L. invasa in the field, determined the density of galls as well as the gall volume on these tree species, and monitored the population dynamics of wasps in Hainan and Guangdong provinces of China. The order of susceptibility to L. invasa was Eucalyptus urophyllaxEucalyptus camaldulensis〉E, urophylla (coppices)〉Eucalyptus exserta〉 Eucalyptus grandisxE, urophylla in Hainan, and Eucalyptus propinqua〉Eucalyptus saligna〉E, exserta〉Eucalyptus microcorys〉Eucahetus dunnii〉E, camaldulensis〉Eucalyptus tereticornis〉Eucalyptus robust in Guangdong, China. Although L. invasa generally damages the midribs and petioles of young leaves and the tender bark of twigs of eucalyptus, galls were not observed on leaves of E. microcorys, E. camaldulensis, or E. dunnii. Gall volume significantly differed among the tree species, and gall volume and wasp number were positively correlated. In Dongfang, Hainan Province, the overwintering period of L. invasa emergencing through the year was from the end of December to March of the next year, and the number of population was the greatest on E. urophylla^E, camaldulensis, and the smallest on E. grandis E. urophylla. In Guangzhou, Guangdong Province, L. invasa hardly emerged in winter from December to June of the next year, and the population was the greatest on E. propinqua, and the smallest on E. microcorys.展开更多
The present study was carried out at Punjab Agricultural University,Ludhiana,India.Nineteen clones of Eucalyptus spp.were evaluated for tolerance to gall wasp.The total number of galls per plant were recorded at 1 mon...The present study was carried out at Punjab Agricultural University,Ludhiana,India.Nineteen clones of Eucalyptus spp.were evaluated for tolerance to gall wasp.The total number of galls per plant were recorded at 1 month after planting(MAP) and the number of galls per branch were recorded at 4 and 7 MAP.Gall wasp incidence(number of galls) was recorded on a whole plant basis during the seedling stage and the number of galls were recorded from the upper,middle and lower crown places at 4 and 7 MAP.Categorization was done on the basis of relative gall-wasp damage by keeping in view the total number of galls per plant and branch(leaves and petiole).No clone was found free from gall damage.Clones C-526,P-23 and P-103 were the least attacked by the gall wasp.Incidence as indicated by number of galls caused by Leptocybe invasa on eucalypts was higher in March than in December.展开更多
AIM: To investigate the presence and potency of natural enzyme inhibitors with hypoglycemic potentials amongst Eucalyptus Spp. by in vitro assays.METHODS: The leaf extracts of the three different Eucalyptus species [E...AIM: To investigate the presence and potency of natural enzyme inhibitors with hypoglycemic potentials amongst Eucalyptus Spp. by in vitro assays.METHODS: The leaf extracts of the three different Eucalyptus species [E. globulus(EG), E. citriodora(EC), E. camaldulensis(ECA)] were subjected to in vitro assay procedures to explore the prevalence of natural enzyme inhibitors(NEIs) after preliminary qualitative and quantitative phytochemical evaluations, to study their inhibitory actions against the enzymes like α-amylase, α-glucosidase, aldose reductase, angiotensin converting enzyme and dipeptidyl peptidase 4 playing pathogenic roles in type 2 diabetes. The antioxidant potential and total antioxidant capacity of the species were also evaluated.RESULTS: Major bioactive compounds like polyphenols(341.75 ± 3.63 to 496.85 ± 3.98) and flavonoids(4.89 ± 0.01 to 7.15 ± 0.02) were found in appreciable quantity in three species. Based on the IC50 values of the extracts under investigation, in all assays the effectivity was in the order of EG > ECA > EC. The results of the ferric reducing antioxidant power assay showed that the reducing ability of the species was also in the order of EG > ECA > EC. A strong correlation(R2 = 0.81-0.99) was found between the phenolic contents and the inhibitory potentials of the extracts against the targeted enzymes.CONCLUSION: These results show immense hypoglycemic potentiality of the Eucalyptus Spp. and a remarkable source of NEIs for a future phytotherapeutic approach in Type 2 diabetes.展开更多
Jute mallow is a nutritious leafy vegetable. The leaves are rich in proteins, vitamins and essential amino acids. Molecular characterization of Jute mallow with focus on improvement of leaf yield is scarcely reported....Jute mallow is a nutritious leafy vegetable. The leaves are rich in proteins, vitamins and essential amino acids. Molecular characterization of Jute mallow with focus on improvement of leaf yield is scarcely reported. In the present study, inter sequence simple repeats (ISSR) molecular markers were employed to assess genetic diversity and relationships of 83 accessions of Jute mallow from different parts of Africa and Asia conserved at the World Vegetable Center East and Southern Africa. A total of 89 bands were amplified by 8 ISSR primers. Number of polymorphic bands per primer ranged from 2 to 6 with an average of 2.75 bands per primer. Polymorphic information content (PIC) values ranged from 0.390 to 0.760 with average of 0.53. Average Nei’s gene diversity (h) and Shannon’s information index (I) were 0.335 and 0.494 respectively. The highest pairwise genetic distance was 0.431 observed in a population from East Africa accessions. PC1 and PC2 axis explained 21.69% and 11.66% of the total variation respectively. UPGMA cluster analysis grouped the accessions into six main clusters at genetic similarity coefficient of 0.53 as standard value for classification. These results have important implications for jute mallow breeding and conservation.展开更多
Bio-jarosite,an iron mineral synthesized biologically using bacteria,is a substitute for iron catalysts in the Fenton oxidation of organic pollutants.Iron nanocatalysts have been widely used as Fenton catalysts becaus...Bio-jarosite,an iron mineral synthesized biologically using bacteria,is a substitute for iron catalysts in the Fenton oxidation of organic pollutants.Iron nanocatalysts have been widely used as Fenton catalysts because they have a larger surface area than ordinary catalysts,are highly recyclable,and can be treated efficiently.This study aimed to explore the catalytic properties of bio-jarosite iron nanoparticles syn-thesized with green methods using two distinct plant species:Azadirachta indica and Eucalyptus gunni.The focus was on the degradation of dicamba via Fenton oxidation.The synthesized nanoparticles exhibited different particle size,shape,surface area,and chemical composition characteristics.Both particles were effective in removing dicamba,with removal efficiencies of 96.8%for A.indica bio-jarosite iron nano-particles(ABFeNPs)and 93.0%for E.gunni bio-jarosite iron nanoparticles(EBFeNPs)within 120 min of treatment.Increasing the catalyst dosage by 0.1 g/L resulted in 7.6%and 43.0%increases in the dicamba removal efficiency for EBFeNPs and ABFeNPs with rate constants of 0.025 min^(-1) and 0.023 min^(-1),respectively,confrming their catalytic roles.Additionally,the high efficiency of both catalysts was demonstrated through five consecutive cycles of linear pseudo-first-order Fenton oxidation reactions.展开更多
Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resi...Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resistance necessitates updating diagnostic techniques to ensure higher sensitivity and specificity, especially with advancements in science and medicine. This study aimed to evaluate the prevalence of UTIs and antibiotic resistance profiles through urine culture, as well as to identify Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp. in urine samples using a molecular approach with multiplex real-time PCR. From May 3 to July 25, 2023, at the Pietro Annigoni Biomolecular Research Center (CERBA) and Saint Camille Hospital of Ouagadougou (HOSCO), 209 urine samples collected from patients with suspected UTIs were analyzed using both urine culture and multiplex real-time PCR. Among the 209 patients, 52.15% were male and 47.85% female, with an average age of 46.87 ± 21.33 years. Urine cultures revealed an overall UTI prevalence of 23.44%, with a prevalence of 8.13% in men versus 15.31% in women (P = 0.023). The bacterial prevalence rates were as follows: Escherichia coli (12.92%), Klebsiella spp. (7.18%), Enterobacter cloacae (1.44%), Staphylococcus aureus (0.96%), and other bacteria. Klebsiella spp. demonstrated 100% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, while Escherichia coli showed 96.2% and 65.4% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, respectively. PCR analysis of the target bacteria revealed mono-infection prevalence rates of Klebsiella pneumoniae (10.39%), Klebsiella oxytoca (7.79%), and Acinetobacter spp. (7.79%), along with a co-infection prevalence rate of Klebsiella pneumoniae/Acinetobacter spp. (1.30%). This study demonstrated that PCR, with its high sensitivity and specificity, could effectively distinguish Klebsiella pneumoniae from Klebsiella oxytoca and detect Acinetobacter spp. in less than 24 hours—something urine culture alone could not achieve. The relative ease of automating urine PCR testing, combined with its diagnostic accuracy and rapid turnaround time, makes it a valuable addition to modern medical practice for the laboratory diagnosis of UTIs.展开更多
Understan ding the mechanism of harmful algal bloom formation is vital for effectively preventing algal bloom outbreaks in coastal environments.Karenia spp.blooms in the East China Sea show a significant correlation w...Understan ding the mechanism of harmful algal bloom formation is vital for effectively preventing algal bloom outbreaks in coastal environments.Karenia spp.blooms in the East China Sea show a significant correlation with nutrient regimes.However,the impact of key components of nutrients,especially dissolved organic nitrogen(DON),on the blooms of Karenia spp.is not clear.Quantitative research is still lacking.In this study,the cruise observations,field mesocosm-flask culture experiments,and a multinitrogen-tri-phytoplankton-detritus model(NTPD) are combined to reveal the quantitative influence of nutrient regimes on the shift of Prorocentrum donghaiense and Karenia spp.in the East China Sea.It has a synchronism rhythm of diatom-P.donghaienseKarenia spp.-diatom loop in the field culture experiment,which is consistent with the results of the cruise observation.The results showed that the processes of terrigenous DON(TeDON) and dissolved inorganic nitrogen(DIN:NO_(3)^(-)-N,NH_(4)^(+)-N) absorption promoted P.donghaiense to become the dominant algae in the community;whereas the processes of DON from P.donghaiense absorption promoted Karenia spp.to become the dominant algae in ambient DIN exhaustion.In addition,the three-dimensional fluorescence components of humus C,tyrosine and fulvic acid can indicate the processes of growth and extinction of P.donghaiense and Karenia spp.,respectively.This study infers that P.donghaiense and Karenia spp.regime shift mechanism associated with the nutrient regime in coastal waters,which provides a scientific basis for the environmental management of coastal eco system health.展开更多
Strawberry (Fragaria spp.) is one of the most important fruits classified as exotic fruits imported into Cameroon. To have an inventory of its cultivation in Cameroon, a survey study was carried out among eight farms ...Strawberry (Fragaria spp.) is one of the most important fruits classified as exotic fruits imported into Cameroon. To have an inventory of its cultivation in Cameroon, a survey study was carried out among eight farms of Fragaria spp. from January 2021 to February 2022. The plant was introduced in Cameroon in 2018. There are 13 varieties of Fragaria spp. currently cultivated. Among these 13 varieties, eleven are hybrids of Fragaria x ananassa (“Amiga”, “Amine”, “Camarosa”, “Chandler”, “Charlotte”, “Elsanta”, “Gariguette”, “Madame Moutot”, “Ostara”, “Ruby gem” and “San Andreas”), and two of the hybrids of Fragaria vesca (“Maestro” and “Mara des bois”). The cropping system, irrigation system, and type of fertilizers applied differ from one strawberry farm to another. Biofertilizers (such as mycorrhizal), inorganic and organic fertilizers are actually used to improve production. The potential annual production of strawberries from January 2021 to February 2022, estimated based on the survey data, was 21.216 tons for all growers. Among these eight production farms, the Lolodorf BIO Farm presents 6000 kg (six tons) of strawberries and 100,000 stolons (seedlings) produced, from seven varieties of Fragaria spp. cultivated, with 6 varieties which are hybrids variety Fragaria x ananassa (“Amiga”, “Amine”, “Chandler”, “Gariguette”, “Madame Moutot”, and “Ruby gem”), and one which is a hybrid of Fragaria vesca (“Mara des bois”). Certain diseases were also observed and recorded depending on the growing areas.展开更多
BACKGROUND Neuropathic pain(NP)is the primary symptom of various neurological condi-tions.Patients with NP often experience mood disorders,particularly depression and anxiety,that can severely affect their normal live...BACKGROUND Neuropathic pain(NP)is the primary symptom of various neurological condi-tions.Patients with NP often experience mood disorders,particularly depression and anxiety,that can severely affect their normal lives.Microglial cells are as-sociated with NP.Excessive inflammatory responses,especially the secretion of large amounts of pro-inflammatory cytokines,ultimately lead to neuroinflam-mation.Microglial pyroptosis is a newly discovered form of inflammatory cell death associated with immune responses and inflammation-related diseases of the central nervous system.METHODS Two models,an in vitro lipopolysaccharide(LPS)-stimulated microglial cell model and a selective nerve injury model using BTX-A and SPP1 knockdown treatments,were used.Key proteins in the pyroptosis signaling pathway,NLRP3-GSDMD,were assessed using western blotting,real-time quantitative polymerase chain reaction,and immunofluorescence.Inflammatory factors[interleukin(IL)-6,IL-1β,and tumor necrosis factor(TNF)-α]were assessed using enzyme-linked immuno-sorbent assay.We also evaluated microglial cell proliferation and apoptosis.Furthermore,we measured pain sensation by assessing the delayed hind paw withdrawal latency using thermal stimulation.RESULTS The expression levels of ACS and GSDMD-N and the mRNA expression of TNF-α,IL-6,and IL-1βwere enhanced in LPS-treated microglia.Furthermore,SPP1 expression was also induced in LPS-treated microglia.Notably,BTX-A inhibited SPP1 mRNA and protein expression in the LPS-treated microglia.Additionally,depletion of SPP1 or BTX-A inhibited cell viability and induced apoptosis in LPS-treated microglia,whereas co-treatment with BTX-A enhanced the effect of SPP1 short hairpin(sh)RNA in LPS-treated microglia.Finally,SPP1 depletion or BTX-A treatment reduced the levels of GSDMD-N,NLPRP3,and ASC and suppressed the production of inflammatory factors.CONCLUSION Notably,BTX-A therapy and SPP1 shRNA enhance microglial proliferation and apoptosis and inhibit microglial death.It improves pain perception and inhibits microglial activation in rats with selective nerve pain.展开更多
Afforestation and reforestation are useful approaches to improve carbon sequestration. With the advent of forest plantations, growing environment conditions have become increasingly restrictive for light, soil nutrien...Afforestation and reforestation are useful approaches to improve carbon sequestration. With the advent of forest plantations, growing environment conditions have become increasingly restrictive for light, soil nutrients, and interactions between trees to acquire available resources. Tree biomass data are essential for understanding the forest carbon cycle and plant adaptations to the environment. The distribution of tree biomass depends on the sum of multiple stand conditions. The data are from a dedicated experiment with two very contrasting areas of fertility, and two planting densities, including a high density at planting in order to achieve thinning. The plant material consists of the high-performance clones of Eucalyptus urophylla × E. grandis and the reference clone E. PF1. We hypothesize that the distribution of biomass changes as the intensity of competition changes and that this is accelerated by the fertility of the sites in time. The results indicate that fertilization, planting density and clones have an impact on biomass partitioning.展开更多
Soil ploughing is an important stage in the preparation of planting, causing disturbance to the physical, chemical and biological properties of the soil. Soil ploughing can affect the availability of nutrients and wat...Soil ploughing is an important stage in the preparation of planting, causing disturbance to the physical, chemical and biological properties of the soil. Soil ploughing can affect the availability of nutrients and water resources, and its effect can be short, medium or long-term. Soil ploughing accelerates surface heating and air circulation and encourages mineralisation by transforming organic matter into mineral salts, making nutrients soluble and accessible to plants. The aim of this study is to determine how soil ploughing affects the distribution of nutrients in the soil profile. The study focuses on nitrogen, carbon, phosphorus, calcium and magnesium, which are major elements of soil fertility on the Batéké plateaux in Congo. The results indicate that ploughing significantly modifies the distribution at depth des elements nutritifs: there is more accumulation at the surface than at depth (ei: nitrogen 1.34 t/ha ± 0.035 at 10 cm compared with 1.034 t/ha ± 0.098 at 50 cm) with a higher concentration of carbon (13.89 t/ha ± 0.87) followed by nitrogen (1.34 t/ha ± 0.035).展开更多
Resistant bacteria can be transmitted to humans through feces or contaminated meat from local chickens. Bacterial strains were isolated from the intestinal contents of 400 local chicken samples from various sales site...Resistant bacteria can be transmitted to humans through feces or contaminated meat from local chickens. Bacterial strains were isolated from the intestinal contents of 400 local chicken samples from various sales sites. These strains were then characterized using bacteriological and biochemical methods to identify resistant strains. In a study conducted in Ouagadougou, we systematically collected chicken fecal samples from 20 locations across the city, followed by isolation and identification of Salmonella spp. using specific enrichment and culture methods, as well as Escherichia coli. Bacterial strains were characterized using antibiotic resistance profiles were determined through agar diffusion tests, revealing sensitivity or resistance to a range of antibiotics based on established scientific criteria. The results showed that out of the 400 samples collected, 81.25% and 63.5% were contaminated by Escherichia coli and Salmonella spp., respectively. Among these, 86.15% of identified Escherichia coli and 50.78% of Salmonella spp. displayed resistance to at least one tested antibiotic. Among 280 Escherichia coli isolates identified resistant to at least one antibiotic, 31.07% were resistant to cefotaxime (CTX), 20.35% to ceftazidime (CAZ), 21.07% to ceftriaxone (CTR), 75% to amoxicillin clavulanic acid (AMC), 23.57% aztreoname (ATM) and 27.14% were resistant to imipenem (IMP). In the case of the 129 Salmonella spp. isolates resistant to at least one tested antibiotic, 34.88% were resistant to CTX;41.08% to CAZ;35.65% to CTR, 92% to AMC, 39.53% to ATM and finally 47.28% were resistant to IMP. Our study revealed high prevalence of resistance in bacterial strains isolated from local chickens sold outdoors in Ouagadougou. These findings raise significant public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.展开更多
Management of the Pointe-Noire Forest requires high-performance tools for simulating tree and stand growth and assessing the sustainability of plantations. Modelling the dynamics of even-aged and mono-species stands i...Management of the Pointe-Noire Forest requires high-performance tools for simulating tree and stand growth and assessing the sustainability of plantations. Modelling the dynamics of even-aged and mono-species stands is a very active research topic. The approaches adopted by researchers vary according to the objectives and species considered: dendrometrical, Eco physiological or architectural. Thanks to the particular nature of these plantations and the trial set-up, it will be possible to explore the various aspects of production, clearly separating the part linked to genetics (three clones tested) from the part linked to the environment (via fertilisation) and the part associated with competition between trees (via planting densities and thinning regimes). This study will make a major contribution to the applicability of the self-thinning line and the RDI (Reineke Density Index) (Reineke, 1933) to fast-growing plantations. This research work will contribute to two points: 1) product diversification, which is a way of coping with international variations in timber markets, and 2) understanding how ecosystems function in exceptionally poor conditions, which will then enable the environmental impacts of the various recommended silvicultural itineraries to be assessed. The results obtained show that competition between trees in a stand of eucalyptus at very high density (10,000 stems/ha) and in two environments of very contrasting fertility is different depending on the clone. The decision on the date of the first thinning with a view to silviculture for timber and energy wood, which aims to ensure sustained and sustainable production of eucalyptus wood in these soils, should be taken between 12 and 14 months. The competition band is strong between 14 and 17 months, when the RDI = 0.8 is double that observed at 12 months.展开更多
基金Subsist by the National Natural Science foundation of China
文摘This paper deals with the photosynthetic properties of Eucalyptue camphoia. E. camaldulenaia. E. gunni-i. E. viminalia. E. giandia. E. dunii and E. maidenii. including the daily bariations of photosynthetic and respiratory rates, the comparison of the photosynthetic rates of various Eucalyriua spp. measured,the relationship between photo-synthetic rate and chlorophyll content, as well as the light saturation point.
基金supported by the Forest Special Fund of Guangdong Province for Technological Innovation Project, China(2010KJCX015-01)
文摘The blue gum chalcid, Leptocybe invasa Fisher & La Salle, invaded China in 2007 and has subsequently caused substantial damage to eucalyptus trees. In the current paper, we investigated the susceptibility of 10 Eucalyptus spp. and Eucahetus dunnii to L. invasa in the field, determined the density of galls as well as the gall volume on these tree species, and monitored the population dynamics of wasps in Hainan and Guangdong provinces of China. The order of susceptibility to L. invasa was Eucalyptus urophyllaxEucalyptus camaldulensis〉E, urophylla (coppices)〉Eucalyptus exserta〉 Eucalyptus grandisxE, urophylla in Hainan, and Eucalyptus propinqua〉Eucalyptus saligna〉E, exserta〉Eucalyptus microcorys〉Eucahetus dunnii〉E, camaldulensis〉Eucalyptus tereticornis〉Eucalyptus robust in Guangdong, China. Although L. invasa generally damages the midribs and petioles of young leaves and the tender bark of twigs of eucalyptus, galls were not observed on leaves of E. microcorys, E. camaldulensis, or E. dunnii. Gall volume significantly differed among the tree species, and gall volume and wasp number were positively correlated. In Dongfang, Hainan Province, the overwintering period of L. invasa emergencing through the year was from the end of December to March of the next year, and the number of population was the greatest on E. urophylla^E, camaldulensis, and the smallest on E. grandis E. urophylla. In Guangzhou, Guangdong Province, L. invasa hardly emerged in winter from December to June of the next year, and the population was the greatest on E. propinqua, and the smallest on E. microcorys.
文摘The present study was carried out at Punjab Agricultural University,Ludhiana,India.Nineteen clones of Eucalyptus spp.were evaluated for tolerance to gall wasp.The total number of galls per plant were recorded at 1 month after planting(MAP) and the number of galls per branch were recorded at 4 and 7 MAP.Gall wasp incidence(number of galls) was recorded on a whole plant basis during the seedling stage and the number of galls were recorded from the upper,middle and lower crown places at 4 and 7 MAP.Categorization was done on the basis of relative gall-wasp damage by keeping in view the total number of galls per plant and branch(leaves and petiole).No clone was found free from gall damage.Clones C-526,P-23 and P-103 were the least attacked by the gall wasp.Incidence as indicated by number of galls caused by Leptocybe invasa on eucalypts was higher in March than in December.
文摘AIM: To investigate the presence and potency of natural enzyme inhibitors with hypoglycemic potentials amongst Eucalyptus Spp. by in vitro assays.METHODS: The leaf extracts of the three different Eucalyptus species [E. globulus(EG), E. citriodora(EC), E. camaldulensis(ECA)] were subjected to in vitro assay procedures to explore the prevalence of natural enzyme inhibitors(NEIs) after preliminary qualitative and quantitative phytochemical evaluations, to study their inhibitory actions against the enzymes like α-amylase, α-glucosidase, aldose reductase, angiotensin converting enzyme and dipeptidyl peptidase 4 playing pathogenic roles in type 2 diabetes. The antioxidant potential and total antioxidant capacity of the species were also evaluated.RESULTS: Major bioactive compounds like polyphenols(341.75 ± 3.63 to 496.85 ± 3.98) and flavonoids(4.89 ± 0.01 to 7.15 ± 0.02) were found in appreciable quantity in three species. Based on the IC50 values of the extracts under investigation, in all assays the effectivity was in the order of EG > ECA > EC. The results of the ferric reducing antioxidant power assay showed that the reducing ability of the species was also in the order of EG > ECA > EC. A strong correlation(R2 = 0.81-0.99) was found between the phenolic contents and the inhibitory potentials of the extracts against the targeted enzymes.CONCLUSION: These results show immense hypoglycemic potentiality of the Eucalyptus Spp. and a remarkable source of NEIs for a future phytotherapeutic approach in Type 2 diabetes.
文摘Jute mallow is a nutritious leafy vegetable. The leaves are rich in proteins, vitamins and essential amino acids. Molecular characterization of Jute mallow with focus on improvement of leaf yield is scarcely reported. In the present study, inter sequence simple repeats (ISSR) molecular markers were employed to assess genetic diversity and relationships of 83 accessions of Jute mallow from different parts of Africa and Asia conserved at the World Vegetable Center East and Southern Africa. A total of 89 bands were amplified by 8 ISSR primers. Number of polymorphic bands per primer ranged from 2 to 6 with an average of 2.75 bands per primer. Polymorphic information content (PIC) values ranged from 0.390 to 0.760 with average of 0.53. Average Nei’s gene diversity (h) and Shannon’s information index (I) were 0.335 and 0.494 respectively. The highest pairwise genetic distance was 0.431 observed in a population from East Africa accessions. PC1 and PC2 axis explained 21.69% and 11.66% of the total variation respectively. UPGMA cluster analysis grouped the accessions into six main clusters at genetic similarity coefficient of 0.53 as standard value for classification. These results have important implications for jute mallow breeding and conservation.
文摘Bio-jarosite,an iron mineral synthesized biologically using bacteria,is a substitute for iron catalysts in the Fenton oxidation of organic pollutants.Iron nanocatalysts have been widely used as Fenton catalysts because they have a larger surface area than ordinary catalysts,are highly recyclable,and can be treated efficiently.This study aimed to explore the catalytic properties of bio-jarosite iron nanoparticles syn-thesized with green methods using two distinct plant species:Azadirachta indica and Eucalyptus gunni.The focus was on the degradation of dicamba via Fenton oxidation.The synthesized nanoparticles exhibited different particle size,shape,surface area,and chemical composition characteristics.Both particles were effective in removing dicamba,with removal efficiencies of 96.8%for A.indica bio-jarosite iron nano-particles(ABFeNPs)and 93.0%for E.gunni bio-jarosite iron nanoparticles(EBFeNPs)within 120 min of treatment.Increasing the catalyst dosage by 0.1 g/L resulted in 7.6%and 43.0%increases in the dicamba removal efficiency for EBFeNPs and ABFeNPs with rate constants of 0.025 min^(-1) and 0.023 min^(-1),respectively,confrming their catalytic roles.Additionally,the high efficiency of both catalysts was demonstrated through five consecutive cycles of linear pseudo-first-order Fenton oxidation reactions.
文摘Urinary tract infections (UTIs) caused by uropathogens are a significant public health problem, and their treatment primarily relies on antibiotic therapy. However, the increasing global development of antibiotic resistance necessitates updating diagnostic techniques to ensure higher sensitivity and specificity, especially with advancements in science and medicine. This study aimed to evaluate the prevalence of UTIs and antibiotic resistance profiles through urine culture, as well as to identify Klebsiella pneumoniae, Klebsiella oxytoca, and Acinetobacter spp. in urine samples using a molecular approach with multiplex real-time PCR. From May 3 to July 25, 2023, at the Pietro Annigoni Biomolecular Research Center (CERBA) and Saint Camille Hospital of Ouagadougou (HOSCO), 209 urine samples collected from patients with suspected UTIs were analyzed using both urine culture and multiplex real-time PCR. Among the 209 patients, 52.15% were male and 47.85% female, with an average age of 46.87 ± 21.33 years. Urine cultures revealed an overall UTI prevalence of 23.44%, with a prevalence of 8.13% in men versus 15.31% in women (P = 0.023). The bacterial prevalence rates were as follows: Escherichia coli (12.92%), Klebsiella spp. (7.18%), Enterobacter cloacae (1.44%), Staphylococcus aureus (0.96%), and other bacteria. Klebsiella spp. demonstrated 100% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, while Escherichia coli showed 96.2% and 65.4% resistance to Amoxicillin and Amoxicillin/Clavulanic Acid, respectively. PCR analysis of the target bacteria revealed mono-infection prevalence rates of Klebsiella pneumoniae (10.39%), Klebsiella oxytoca (7.79%), and Acinetobacter spp. (7.79%), along with a co-infection prevalence rate of Klebsiella pneumoniae/Acinetobacter spp. (1.30%). This study demonstrated that PCR, with its high sensitivity and specificity, could effectively distinguish Klebsiella pneumoniae from Klebsiella oxytoca and detect Acinetobacter spp. in less than 24 hours—something urine culture alone could not achieve. The relative ease of automating urine PCR testing, combined with its diagnostic accuracy and rapid turnaround time, makes it a valuable addition to modern medical practice for the laboratory diagnosis of UTIs.
基金The National Natural Science Foundation of China under contract No.42130403the Fundamental Research Funds for the Central Universities under contract No.202362003the National Key Research&Development Program of China under contract No.2017YFC1404300。
文摘Understan ding the mechanism of harmful algal bloom formation is vital for effectively preventing algal bloom outbreaks in coastal environments.Karenia spp.blooms in the East China Sea show a significant correlation with nutrient regimes.However,the impact of key components of nutrients,especially dissolved organic nitrogen(DON),on the blooms of Karenia spp.is not clear.Quantitative research is still lacking.In this study,the cruise observations,field mesocosm-flask culture experiments,and a multinitrogen-tri-phytoplankton-detritus model(NTPD) are combined to reveal the quantitative influence of nutrient regimes on the shift of Prorocentrum donghaiense and Karenia spp.in the East China Sea.It has a synchronism rhythm of diatom-P.donghaienseKarenia spp.-diatom loop in the field culture experiment,which is consistent with the results of the cruise observation.The results showed that the processes of terrigenous DON(TeDON) and dissolved inorganic nitrogen(DIN:NO_(3)^(-)-N,NH_(4)^(+)-N) absorption promoted P.donghaiense to become the dominant algae in the community;whereas the processes of DON from P.donghaiense absorption promoted Karenia spp.to become the dominant algae in ambient DIN exhaustion.In addition,the three-dimensional fluorescence components of humus C,tyrosine and fulvic acid can indicate the processes of growth and extinction of P.donghaiense and Karenia spp.,respectively.This study infers that P.donghaiense and Karenia spp.regime shift mechanism associated with the nutrient regime in coastal waters,which provides a scientific basis for the environmental management of coastal eco system health.
文摘Strawberry (Fragaria spp.) is one of the most important fruits classified as exotic fruits imported into Cameroon. To have an inventory of its cultivation in Cameroon, a survey study was carried out among eight farms of Fragaria spp. from January 2021 to February 2022. The plant was introduced in Cameroon in 2018. There are 13 varieties of Fragaria spp. currently cultivated. Among these 13 varieties, eleven are hybrids of Fragaria x ananassa (“Amiga”, “Amine”, “Camarosa”, “Chandler”, “Charlotte”, “Elsanta”, “Gariguette”, “Madame Moutot”, “Ostara”, “Ruby gem” and “San Andreas”), and two of the hybrids of Fragaria vesca (“Maestro” and “Mara des bois”). The cropping system, irrigation system, and type of fertilizers applied differ from one strawberry farm to another. Biofertilizers (such as mycorrhizal), inorganic and organic fertilizers are actually used to improve production. The potential annual production of strawberries from January 2021 to February 2022, estimated based on the survey data, was 21.216 tons for all growers. Among these eight production farms, the Lolodorf BIO Farm presents 6000 kg (six tons) of strawberries and 100,000 stolons (seedlings) produced, from seven varieties of Fragaria spp. cultivated, with 6 varieties which are hybrids variety Fragaria x ananassa (“Amiga”, “Amine”, “Chandler”, “Gariguette”, “Madame Moutot”, and “Ruby gem”), and one which is a hybrid of Fragaria vesca (“Mara des bois”). Certain diseases were also observed and recorded depending on the growing areas.
文摘BACKGROUND Neuropathic pain(NP)is the primary symptom of various neurological condi-tions.Patients with NP often experience mood disorders,particularly depression and anxiety,that can severely affect their normal lives.Microglial cells are as-sociated with NP.Excessive inflammatory responses,especially the secretion of large amounts of pro-inflammatory cytokines,ultimately lead to neuroinflam-mation.Microglial pyroptosis is a newly discovered form of inflammatory cell death associated with immune responses and inflammation-related diseases of the central nervous system.METHODS Two models,an in vitro lipopolysaccharide(LPS)-stimulated microglial cell model and a selective nerve injury model using BTX-A and SPP1 knockdown treatments,were used.Key proteins in the pyroptosis signaling pathway,NLRP3-GSDMD,were assessed using western blotting,real-time quantitative polymerase chain reaction,and immunofluorescence.Inflammatory factors[interleukin(IL)-6,IL-1β,and tumor necrosis factor(TNF)-α]were assessed using enzyme-linked immuno-sorbent assay.We also evaluated microglial cell proliferation and apoptosis.Furthermore,we measured pain sensation by assessing the delayed hind paw withdrawal latency using thermal stimulation.RESULTS The expression levels of ACS and GSDMD-N and the mRNA expression of TNF-α,IL-6,and IL-1βwere enhanced in LPS-treated microglia.Furthermore,SPP1 expression was also induced in LPS-treated microglia.Notably,BTX-A inhibited SPP1 mRNA and protein expression in the LPS-treated microglia.Additionally,depletion of SPP1 or BTX-A inhibited cell viability and induced apoptosis in LPS-treated microglia,whereas co-treatment with BTX-A enhanced the effect of SPP1 short hairpin(sh)RNA in LPS-treated microglia.Finally,SPP1 depletion or BTX-A treatment reduced the levels of GSDMD-N,NLPRP3,and ASC and suppressed the production of inflammatory factors.CONCLUSION Notably,BTX-A therapy and SPP1 shRNA enhance microglial proliferation and apoptosis and inhibit microglial death.It improves pain perception and inhibits microglial activation in rats with selective nerve pain.
文摘Afforestation and reforestation are useful approaches to improve carbon sequestration. With the advent of forest plantations, growing environment conditions have become increasingly restrictive for light, soil nutrients, and interactions between trees to acquire available resources. Tree biomass data are essential for understanding the forest carbon cycle and plant adaptations to the environment. The distribution of tree biomass depends on the sum of multiple stand conditions. The data are from a dedicated experiment with two very contrasting areas of fertility, and two planting densities, including a high density at planting in order to achieve thinning. The plant material consists of the high-performance clones of Eucalyptus urophylla × E. grandis and the reference clone E. PF1. We hypothesize that the distribution of biomass changes as the intensity of competition changes and that this is accelerated by the fertility of the sites in time. The results indicate that fertilization, planting density and clones have an impact on biomass partitioning.
文摘Soil ploughing is an important stage in the preparation of planting, causing disturbance to the physical, chemical and biological properties of the soil. Soil ploughing can affect the availability of nutrients and water resources, and its effect can be short, medium or long-term. Soil ploughing accelerates surface heating and air circulation and encourages mineralisation by transforming organic matter into mineral salts, making nutrients soluble and accessible to plants. The aim of this study is to determine how soil ploughing affects the distribution of nutrients in the soil profile. The study focuses on nitrogen, carbon, phosphorus, calcium and magnesium, which are major elements of soil fertility on the Batéké plateaux in Congo. The results indicate that ploughing significantly modifies the distribution at depth des elements nutritifs: there is more accumulation at the surface than at depth (ei: nitrogen 1.34 t/ha ± 0.035 at 10 cm compared with 1.034 t/ha ± 0.098 at 50 cm) with a higher concentration of carbon (13.89 t/ha ± 0.87) followed by nitrogen (1.34 t/ha ± 0.035).
文摘Resistant bacteria can be transmitted to humans through feces or contaminated meat from local chickens. Bacterial strains were isolated from the intestinal contents of 400 local chicken samples from various sales sites. These strains were then characterized using bacteriological and biochemical methods to identify resistant strains. In a study conducted in Ouagadougou, we systematically collected chicken fecal samples from 20 locations across the city, followed by isolation and identification of Salmonella spp. using specific enrichment and culture methods, as well as Escherichia coli. Bacterial strains were characterized using antibiotic resistance profiles were determined through agar diffusion tests, revealing sensitivity or resistance to a range of antibiotics based on established scientific criteria. The results showed that out of the 400 samples collected, 81.25% and 63.5% were contaminated by Escherichia coli and Salmonella spp., respectively. Among these, 86.15% of identified Escherichia coli and 50.78% of Salmonella spp. displayed resistance to at least one tested antibiotic. Among 280 Escherichia coli isolates identified resistant to at least one antibiotic, 31.07% were resistant to cefotaxime (CTX), 20.35% to ceftazidime (CAZ), 21.07% to ceftriaxone (CTR), 75% to amoxicillin clavulanic acid (AMC), 23.57% aztreoname (ATM) and 27.14% were resistant to imipenem (IMP). In the case of the 129 Salmonella spp. isolates resistant to at least one tested antibiotic, 34.88% were resistant to CTX;41.08% to CAZ;35.65% to CTR, 92% to AMC, 39.53% to ATM and finally 47.28% were resistant to IMP. Our study revealed high prevalence of resistance in bacterial strains isolated from local chickens sold outdoors in Ouagadougou. These findings raise significant public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.
文摘Management of the Pointe-Noire Forest requires high-performance tools for simulating tree and stand growth and assessing the sustainability of plantations. Modelling the dynamics of even-aged and mono-species stands is a very active research topic. The approaches adopted by researchers vary according to the objectives and species considered: dendrometrical, Eco physiological or architectural. Thanks to the particular nature of these plantations and the trial set-up, it will be possible to explore the various aspects of production, clearly separating the part linked to genetics (three clones tested) from the part linked to the environment (via fertilisation) and the part associated with competition between trees (via planting densities and thinning regimes). This study will make a major contribution to the applicability of the self-thinning line and the RDI (Reineke Density Index) (Reineke, 1933) to fast-growing plantations. This research work will contribute to two points: 1) product diversification, which is a way of coping with international variations in timber markets, and 2) understanding how ecosystems function in exceptionally poor conditions, which will then enable the environmental impacts of the various recommended silvicultural itineraries to be assessed. The results obtained show that competition between trees in a stand of eucalyptus at very high density (10,000 stems/ha) and in two environments of very contrasting fertility is different depending on the clone. The decision on the date of the first thinning with a view to silviculture for timber and energy wood, which aims to ensure sustained and sustainable production of eucalyptus wood in these soils, should be taken between 12 and 14 months. The competition band is strong between 14 and 17 months, when the RDI = 0.8 is double that observed at 12 months.