[Objective] The aim was to study on changing rules of physical and chemical properties of Eucalyptus uraphylla spp. forest at different ages in Southwest of Yunnan Province. [Method] In the research, field survey and ...[Objective] The aim was to study on changing rules of physical and chemical properties of Eucalyptus uraphylla spp. forest at different ages in Southwest of Yunnan Province. [Method] In the research, field survey and laboratory analysis were adopted to study on physical and chemical properties of soils for the Eucalyptus uraphylla spp. at the first cutting ages in Lancang County. [Result] With the increasing of forest ages, for physical properties of soils, soil bulk density was increasing, and soil structure became poor; water contents in soils and capillary porosity were improving, but total soil porosity and non-capillary porosity were decreasing. For chemical properties, pH of soil dropped, but soil acidity improved; organic matter, total N, hydrolysable nitrogen, available phosphorus, and available potassium all declined; exchangeable Ca and Mg were improving due to fertilization and Ca and Mg lower consumption. The results showed that plays important roles in water absorption of soils and vegetation growth were improved with age increase by Eucalyptus uraphylla spp. forest, but ventilation and fertility declined accordingly. [Conclusion] The research indicated that dry branches and fallen leaves should be preserved and nutrient cycling should be protected during introduction and management of Eucalyptus uraphylla spp. to guarantee the nutrients in dry branches and leaves are back to soils, providing theoretical references for management of forest plantation and environment protection.展开更多
Eucalyptus has become an important genus in China because it displays adaptability to a wide range of site conditions and produces pulp wood and veneer on short rotations. The aim of this study was to estimate genetic...Eucalyptus has become an important genus in China because it displays adaptability to a wide range of site conditions and produces pulp wood and veneer on short rotations. The aim of this study was to estimate genetic parameters and relationships and consider impli- cations for development of clonal forestry. We assessed growth traits, stem-branch characteristics, crown diameter and height of fresh branch for 20 Eucalyptus hybrid clones in China measured at the age of 44 months. Analysis of variance showed that there were significant differences in growth traits, height of fresh branch and stem straightness among clones. Significant differences in height, volume, crown diameter, height of fresh branch and branch size among replicates were also recorded. Coefficients of vari- ation ranged from 9.84 to 28.54 % for growth traits, 12.03 to 17.25 % for stem-branch characteristics, 18.26 % for crown diameter and 11.73 % for height of fresh branch. Estimates of repeatabilities for height, diameter at breastheight over bark, volume, crown diameter, height of fresh branch, stem straightness and branch size at clone mean level were 0.86, 0.80, 0.80, 0.54, 0.85, 0.77 and 0.44 respectively. Diameter at breast height over bark and height had strongly positive phenotypic and genotypic correlations with volume, ranging from 0.96 to 1.00. The positive genotypic and phenotypic correlations between growth traits and other studied traits suggested that fast growing clones always had bigger crown diameter, higher height of fresh branch, straighter stems and relatively smaller branches.展开更多
The present study was carried out at Punjab Agricultural University,Ludhiana,India.Nineteen clones of Eucalyptus spp.were evaluated for tolerance to gall wasp.The total number of galls per plant were recorded at 1 mon...The present study was carried out at Punjab Agricultural University,Ludhiana,India.Nineteen clones of Eucalyptus spp.were evaluated for tolerance to gall wasp.The total number of galls per plant were recorded at 1 month after planting（MAP） and the number of galls per branch were recorded at 4 and 7 MAP.Gall wasp incidence（number of galls） was recorded on a whole plant basis during the seedling stage and the number of galls were recorded from the upper,middle and lower crown places at 4 and 7 MAP.Categorization was done on the basis of relative gall-wasp damage by keeping in view the total number of galls per plant and branch（leaves and petiole）.No clone was found free from gall damage.Clones C-526,P-23 and P-103 were the least attacked by the gall wasp.Incidence as indicated by number of galls caused by Leptocybe invasa on eucalypts was higher in March than in December.展开更多
植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用...植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用同源克隆的方法 ,首次分离到PSAG基因的2个成员:PSAG1、PSAG2(GenBank登录号分别为JX317082、JX317083),分别为800、827 bp,分别编码150、160个氨基酸;PSAG1、PSAG2的基因组序列分析表明2个成员均没有内含子。生物信息学分析表明:PSAG1、PSAG2具有PSⅠ的Ⅹ亚基超家族(photosystemⅠreaction center subunitⅩpsaK)保守结构域,是不具有信号肽的跨膜蛋白,具有亲水性;PSAG1、PSAG2均有4个位点发生磷酸化。宦溪野生蕉PSAG在进化过程中形成了特殊的结构特征,即PSAG1和PSAG2没有内含子,并且在不同物种间保守区具有高度的一致性,为保持PSAG功能的稳定性提供了重要保证。展开更多
Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. Most of the disease-resistant genes encode pro- teins with conserved leucine rich repeat and nucle...Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. Most of the disease-resistant genes encode pro- teins with conserved leucine rich repeat and nucleotide binding site domains. In this study, we cloned and char- acterized gene-resistant analogs （RGAs） from Eucalyptus grandis using degenerate PCR, with primers specifically targeting these two domains. The amplified fragments were cloned into the pGEM-T vector and transformed into Escherichia coli. Among the 90 clones obtained, 13 were sequenced and compared with each other and with previ- ously identified gene-resistant diseases. A BLASTX search in GenBank revealed high similarities among the con- served domains of these cloned genes with RGA genes. Some clones, however, showed no significant similarity with DNA sequences in GenBank. Southern blotting ana- lysis identified several polymorphic RFLP loci between distinct genotypes. However, none of them co-segregated with the Puccinia psidii Winter resistance gene 1 （Ppr1） in a population study.展开更多
Eighteen clones of Eucalyptus tereticornis （Sm.） were evaluated for three years by adopting randomized block design for various growth parameters at Hoshiarpur, Punjab, India and compared with two checks. Significan...Eighteen clones of Eucalyptus tereticornis （Sm.） were evaluated for three years by adopting randomized block design for various growth parameters at Hoshiarpur, Punjab, India and compared with two checks. Significant variations were recorded for height, diameter at breast height （DBH） and clear bole height （CBH）. The broad sense heritability was low to moderate for both height and CBH. The genetic gain for height and CBH increased substantially pe se with the increase in age of trees. The average genetic gain for thres years was recorded maximum for height （159.60%） followed by DBH （110.97%） and CBH （70.34%）. Clone 17 attained maximum DBH over other genotypes for second and third year followed by clones 14 and 11. Clone 5 showed an upward trend for DBH and maintained its superiority for CBH as the age of the tree increased. Similarly, clone 11 changed its ranking from 9th to 8th to 3rd for DBH and from 9th to 4th to 2nd for CBH, respectively for the age of one, two and three years. Nonetheless, clones 6 and 10 per- formed poorly for all the characters studied. Clones 17, 14 and 5 were found to be the most promising clones for commercial deployment.展开更多
基金Supported by National Natural Science Foundation of China (40961031)Applied Basic Research Programs of Yunnan Province (2009CD022)Young and Middle-aged Teachers Training of Yunnan University (XT412003)~~
文摘[Objective] The aim was to study on changing rules of physical and chemical properties of Eucalyptus uraphylla spp. forest at different ages in Southwest of Yunnan Province. [Method] In the research, field survey and laboratory analysis were adopted to study on physical and chemical properties of soils for the Eucalyptus uraphylla spp. at the first cutting ages in Lancang County. [Result] With the increasing of forest ages, for physical properties of soils, soil bulk density was increasing, and soil structure became poor; water contents in soils and capillary porosity were improving, but total soil porosity and non-capillary porosity were decreasing. For chemical properties, pH of soil dropped, but soil acidity improved; organic matter, total N, hydrolysable nitrogen, available phosphorus, and available potassium all declined; exchangeable Ca and Mg were improving due to fertilization and Ca and Mg lower consumption. The results showed that plays important roles in water absorption of soils and vegetation growth were improved with age increase by Eucalyptus uraphylla spp. forest, but ventilation and fertility declined accordingly. [Conclusion] The research indicated that dry branches and fallen leaves should be preserved and nutrient cycling should be protected during introduction and management of Eucalyptus uraphylla spp. to guarantee the nutrients in dry branches and leaves are back to soils, providing theoretical references for management of forest plantation and environment protection.
基金finically supported by the National Twelfth Five-Year Science and Technology Plan ‘‘Breeding of High yield and High Resistance New Species of Eucalyptus’’(2012BAD01B0401),‘‘Genetic Research of Pulp Yield and Veneer of Eucalyptus urophylla Hybrid Clones’’(RITFYWZX201304)
文摘Eucalyptus has become an important genus in China because it displays adaptability to a wide range of site conditions and produces pulp wood and veneer on short rotations. The aim of this study was to estimate genetic parameters and relationships and consider impli- cations for development of clonal forestry. We assessed growth traits, stem-branch characteristics, crown diameter and height of fresh branch for 20 Eucalyptus hybrid clones in China measured at the age of 44 months. Analysis of variance showed that there were significant differences in growth traits, height of fresh branch and stem straightness among clones. Significant differences in height, volume, crown diameter, height of fresh branch and branch size among replicates were also recorded. Coefficients of vari- ation ranged from 9.84 to 28.54 % for growth traits, 12.03 to 17.25 % for stem-branch characteristics, 18.26 % for crown diameter and 11.73 % for height of fresh branch. Estimates of repeatabilities for height, diameter at breastheight over bark, volume, crown diameter, height of fresh branch, stem straightness and branch size at clone mean level were 0.86, 0.80, 0.80, 0.54, 0.85, 0.77 and 0.44 respectively. Diameter at breast height over bark and height had strongly positive phenotypic and genotypic correlations with volume, ranging from 0.96 to 1.00. The positive genotypic and phenotypic correlations between growth traits and other studied traits suggested that fast growing clones always had bigger crown diameter, higher height of fresh branch, straighter stems and relatively smaller branches.
文摘The present study was carried out at Punjab Agricultural University,Ludhiana,India.Nineteen clones of Eucalyptus spp.were evaluated for tolerance to gall wasp.The total number of galls per plant were recorded at 1 month after planting（MAP） and the number of galls per branch were recorded at 4 and 7 MAP.Gall wasp incidence（number of galls） was recorded on a whole plant basis during the seedling stage and the number of galls were recorded from the upper,middle and lower crown places at 4 and 7 MAP.Categorization was done on the basis of relative gall-wasp damage by keeping in view the total number of galls per plant and branch（leaves and petiole）.No clone was found free from gall damage.Clones C-526,P-23 and P-103 were the least attacked by the gall wasp.Incidence as indicated by number of galls caused by Leptocybe invasa on eucalypts was higher in March than in December.
文摘植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用同源克隆的方法 ,首次分离到PSAG基因的2个成员:PSAG1、PSAG2(GenBank登录号分别为JX317082、JX317083),分别为800、827 bp,分别编码150、160个氨基酸;PSAG1、PSAG2的基因组序列分析表明2个成员均没有内含子。生物信息学分析表明:PSAG1、PSAG2具有PSⅠ的Ⅹ亚基超家族(photosystemⅠreaction center subunitⅩpsaK)保守结构域,是不具有信号肽的跨膜蛋白,具有亲水性;PSAG1、PSAG2均有4个位点发生磷酸化。宦溪野生蕉PSAG在进化过程中形成了特殊的结构特征,即PSAG1和PSAG2没有内含子,并且在不同物种间保守区具有高度的一致性,为保持PSAG功能的稳定性提供了重要保证。
基金the Suzano Celulose S/A for logistical supportthe‘‘Conselho Nacional de Desenvolvimento Científico e Tecnológico’’(CNPq)+1 种基金the‘‘Coordenao de Aperfeioamento de Pessoal de Nível Superior’’(CAPES)the‘‘Fundao de Amparo à Pesquisa do Estado de Minas Gerais’’(FAPEMIG)for financial support to this study
文摘Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. Most of the disease-resistant genes encode pro- teins with conserved leucine rich repeat and nucleotide binding site domains. In this study, we cloned and char- acterized gene-resistant analogs （RGAs） from Eucalyptus grandis using degenerate PCR, with primers specifically targeting these two domains. The amplified fragments were cloned into the pGEM-T vector and transformed into Escherichia coli. Among the 90 clones obtained, 13 were sequenced and compared with each other and with previ- ously identified gene-resistant diseases. A BLASTX search in GenBank revealed high similarities among the con- served domains of these cloned genes with RGA genes. Some clones, however, showed no significant similarity with DNA sequences in GenBank. Southern blotting ana- lysis identified several polymorphic RFLP loci between distinct genotypes. However, none of them co-segregated with the Puccinia psidii Winter resistance gene 1 （Ppr1） in a population study.
文摘Eighteen clones of Eucalyptus tereticornis （Sm.） were evaluated for three years by adopting randomized block design for various growth parameters at Hoshiarpur, Punjab, India and compared with two checks. Significant variations were recorded for height, diameter at breast height （DBH） and clear bole height （CBH）. The broad sense heritability was low to moderate for both height and CBH. The genetic gain for height and CBH increased substantially pe se with the increase in age of trees. The average genetic gain for thres years was recorded maximum for height （159.60%） followed by DBH （110.97%） and CBH （70.34%）. Clone 17 attained maximum DBH over other genotypes for second and third year followed by clones 14 and 11. Clone 5 showed an upward trend for DBH and maintained its superiority for CBH as the age of the tree increased. Similarly, clone 11 changed its ranking from 9th to 8th to 3rd for DBH and from 9th to 4th to 2nd for CBH, respectively for the age of one, two and three years. Nonetheless, clones 6 and 10 per- formed poorly for all the characters studied. Clones 17, 14 and 5 were found to be the most promising clones for commercial deployment.
文摘目的:克隆悬钩子属植物肌动蛋白基因(actin),为研究该物种中其他基因的表达和调控提供内标基因。方法:利用一对特异性引物从黑莓、悬钩子杂种和树莓品种中克隆actin cDNA片段,对其进行序列分析和半定量RT-PCR表达分析。结果:从3个品种中均获得一条783 bp actin cDNA片段,编码260个氨基酸,3条actin片段与其他植物核苷酸序列的同源性都在82%以上,与其他植物氨基酸序列同源性也均在95%以上;树莓和悬钩子杂种品种的actin核苷酸和氨基酸序列同源性均达99%,系统进化分析也发现两者亲缘关系较近些;表达分析发现actin基因在各品种不同组织中均有一定的表达量。结论:首次克隆了悬钩子属植物肌动蛋白基因actin,将来自黑莓和树莓品种的序列登录在GenBank,登录号分别为HQ439556和HQ439557。
