Soil microbes may be critical players in determining the allelopathic potential of some plants. Low levels of plant community biodiversity in Eucalyptus plantations have been attributed to the allelopathic potential o...Soil microbes may be critical players in determining the allelopathic potential of some plants. Low levels of plant community biodiversity in Eucalyptus plantations have been attributed to the allelopathic potential of these tree species. The role of soil microbes in the allelopathic effect of leaf leachates of the hybrid tree Eucalyptus grandis x E. urophylla, was tested in Petri dish assays with Brassica chinensis as a receiver plant. Soils were collected from either a local garden (soil A) or a Eucalyptus plantation (soil B) and half of each soil was sterilized to remove microbes. These soils were then treated with E. grandis x E. urophylla leachates for 0-72 h. Seed germination of B. chinensis was significantly inhibited in soils treated with leaf leachates relative to untreated soils. The inhibitory effect of the leaf leachates was more pronounced in sterilized soils. Total phenolic content was obvious lower in nonsterile leachate-treated soils than in sterile soils. Biomass of B. chinensis was negatively correlated with the total phenolic content in soils. Our findings suggest that soil microbes can alleviate the allelopathic potential of Eucalyptus and thereby its negative impact on plant growth.展开更多
用CO(NH_2)_2和Na_2SO_4模拟氮-硫沉降,采用二因素三水平法对氮-硫单一及复合沉降6个月后尾巨桉(Eucalyptus urophylla S.T.Blake×E.grandis Hill ex Maiden)和杉木〔Cunninghamia lanceolata(Lamb.)Hook.〕幼苗根、茎、叶片和全...用CO(NH_2)_2和Na_2SO_4模拟氮-硫沉降,采用二因素三水平法对氮-硫单一及复合沉降6个月后尾巨桉(Eucalyptus urophylla S.T.Blake×E.grandis Hill ex Maiden)和杉木〔Cunninghamia lanceolata(Lamb.)Hook.〕幼苗根、茎、叶片和全株中的全氮和全磷含量及叶片N∶P比的变化进行比较;在此基础上,分析了各器官间以及各器官与全株间全氮和全磷含量的相关性。对尾巨桉的研究结果表明:低和高水平(50和100 kg·hm^(-2)·a^(-1))单一氮沉降条件下各器官和全株的全氮含量均显著高于对照、叶片N∶P比也高于对照,而各器官和全株的全磷含量总体上与对照无显著差异;在低和高水平(15和30 kg·hm^(-2)·a^(-1))单一硫沉降及氮-硫复合沉降条件下,各器官和全株的全氮含量、全株的全磷含量及叶片的N∶P比大多显著高于对照,而各器官的全磷含量则与对照无显著差异。对杉木的研究结果表明:低和高水平单一氮沉降条件下茎、叶和全株的全氮含量均显著高于对照、根的全氮含量则低于对照,各器官和全株的全磷含量及叶片的N∶P比与对照无显著差异;低和高水平单一硫沉降条件下根、叶片和全株的全氮含量及叶片的N∶P比大多显著高于对照,而茎的全氮含量和全株的全磷含量则显著低于对照,各器官的全磷含量与对照无显著差异;氮-硫复合沉降条件下各器官和全株的全氮含量及叶片的N∶P比大多显著高于对照,而各器官和全株的全磷含量则与对照无显著差异。从全株的全氮和全磷含量以及叶片N∶P比的变幅看,2个树种全株的全氮含量对氮-硫复合沉降的响应最强。此外,氮-硫沉降条件下2个树种各器官间的全氮和全磷含量总体无显著相关性,仅尾巨桉根与叶片的全磷含量呈显著正相关;而尾巨桉各器官与全株的全氮和全磷含量呈显著或极显著正相关,杉木叶片与全株的全氮和全磷含量及其茎与全株的全磷含量均呈显著正相关。研究结果显示:在一定水平的短期氮-硫沉降条件下2个树种幼苗的氮和磷含量均增加,其中氮含量对氮-硫复合沉降的响应效应最强、磷含量对单一氮沉降的响应效应最强;尾巨桉对氮-硫沉降的响应效应强于杉木,且其生长限制元素呈现由氮限制转向氮-磷共同限制的趋势。展开更多
基金supported by the Sci-technology program of Guangxi Province(No.Guikezhong 1347001)
文摘Soil microbes may be critical players in determining the allelopathic potential of some plants. Low levels of plant community biodiversity in Eucalyptus plantations have been attributed to the allelopathic potential of these tree species. The role of soil microbes in the allelopathic effect of leaf leachates of the hybrid tree Eucalyptus grandis x E. urophylla, was tested in Petri dish assays with Brassica chinensis as a receiver plant. Soils were collected from either a local garden (soil A) or a Eucalyptus plantation (soil B) and half of each soil was sterilized to remove microbes. These soils were then treated with E. grandis x E. urophylla leachates for 0-72 h. Seed germination of B. chinensis was significantly inhibited in soils treated with leaf leachates relative to untreated soils. The inhibitory effect of the leaf leachates was more pronounced in sterilized soils. Total phenolic content was obvious lower in nonsterile leachate-treated soils than in sterile soils. Biomass of B. chinensis was negatively correlated with the total phenolic content in soils. Our findings suggest that soil microbes can alleviate the allelopathic potential of Eucalyptus and thereby its negative impact on plant growth.
文摘用CO(NH_2)_2和Na_2SO_4模拟氮-硫沉降,采用二因素三水平法对氮-硫单一及复合沉降6个月后尾巨桉(Eucalyptus urophylla S.T.Blake×E.grandis Hill ex Maiden)和杉木〔Cunninghamia lanceolata(Lamb.)Hook.〕幼苗根、茎、叶片和全株中的全氮和全磷含量及叶片N∶P比的变化进行比较;在此基础上,分析了各器官间以及各器官与全株间全氮和全磷含量的相关性。对尾巨桉的研究结果表明:低和高水平(50和100 kg·hm^(-2)·a^(-1))单一氮沉降条件下各器官和全株的全氮含量均显著高于对照、叶片N∶P比也高于对照,而各器官和全株的全磷含量总体上与对照无显著差异;在低和高水平(15和30 kg·hm^(-2)·a^(-1))单一硫沉降及氮-硫复合沉降条件下,各器官和全株的全氮含量、全株的全磷含量及叶片的N∶P比大多显著高于对照,而各器官的全磷含量则与对照无显著差异。对杉木的研究结果表明:低和高水平单一氮沉降条件下茎、叶和全株的全氮含量均显著高于对照、根的全氮含量则低于对照,各器官和全株的全磷含量及叶片的N∶P比与对照无显著差异;低和高水平单一硫沉降条件下根、叶片和全株的全氮含量及叶片的N∶P比大多显著高于对照,而茎的全氮含量和全株的全磷含量则显著低于对照,各器官的全磷含量与对照无显著差异;氮-硫复合沉降条件下各器官和全株的全氮含量及叶片的N∶P比大多显著高于对照,而各器官和全株的全磷含量则与对照无显著差异。从全株的全氮和全磷含量以及叶片N∶P比的变幅看,2个树种全株的全氮含量对氮-硫复合沉降的响应最强。此外,氮-硫沉降条件下2个树种各器官间的全氮和全磷含量总体无显著相关性,仅尾巨桉根与叶片的全磷含量呈显著正相关;而尾巨桉各器官与全株的全氮和全磷含量呈显著或极显著正相关,杉木叶片与全株的全氮和全磷含量及其茎与全株的全磷含量均呈显著正相关。研究结果显示:在一定水平的短期氮-硫沉降条件下2个树种幼苗的氮和磷含量均增加,其中氮含量对氮-硫复合沉降的响应效应最强、磷含量对单一氮沉降的响应效应最强;尾巨桉对氮-硫沉降的响应效应强于杉木,且其生长限制元素呈现由氮限制转向氮-磷共同限制的趋势。