New Evidence for the Hypothesis of Reducing Natural Enemy Pressure of Eupatorium adenophorum: Solenopsis invicta Competing with Doxrylus orientalis to Feed on E. adenophorum

Natural enemy insects are important factors in regulating plant invasion. The interactions between multiple insect species resulting from invasive plants remain poorly understood despite their potential. We observed notorious invasive plants Eupatorium adenophorum Sprengel were competedly fed by Solenopsis invicta Buren (Hymenoptera: Formicidae) and Dorylus orientalis Westwood (Hymenoptera: Formicidae) beside the scientific research base of Kunming University. It was first observed that D. orientalis was eating the epidermis, cortex, phloem and other root and stem tissues of E. adenophorum in soil. Two months later, it was observed that S. invicta ate the epidermis, cortex, phloem and other aboveground stem tissues of E. adenophorum. S. invicta attacked D. orientalis and displaced their living space by causing the later dead, injured, or even disabled. This phenomenon suggested that local herbivorous insects have adapted to E. adenophorum well, which will intensify the naturalization process of E. adenophorum in local habitats. In a homogeneous garden planting experiment of E. adenophorum conducted at the experimental base of Kunming University, the biomass of the introduced (China) populations of E. adenophorum was lower than that of the native (Mexico) populations, although there was no statistically significant difference. These results indicate a possible positive correlation between the increase in natural enemy pressure and the decrease in fitness of E. adenophorum.

Review on studies of Eupatorium adenophorum--an important invasive species in China

Eupatorium adenophorum Spreng. was introduced in Yunnan Province of China around 1940. Since then it has been spreading rapidly, particularly in the southern and southwestern parts of China and caused serious economic loss. The biological research and inte-grated control on E. adenophorum were carried out from 1980's in Yunnan Province. Together with other 15 invasive external species, the weed has been listed in the White Paper by The State Environmental Protection Administration of China. This paper briefly reviews the studies on natural distribution, biological character, ecological character, chemical component, hazard, potential application and the control of E. adenophorum. The research direction for this invasive external species in future was also discussed.

Ecological adaptation of Eupatorium adenophorum populations to light intensity

Eupatorium adenophorum is one of main invasive plants in China and has caused great economic losses. A study was conducted to determine the biomass allocation, leaf morphology and growth response of E. adenophorum seedlings that grew under five different intensities （relative irradiances RI 10%, 20 %, 30%, 55%, 100%） for 14 months. Results reveal that the species shows typical leaf morphological adaptation to different light conditions. The total biomass of seedlings increased with the increase of light intensity from 10% to 55% RI but decreased at RI 100% （full sunlight）. Height growth increased with the increase of light intensity from 10% to 30% RI but decreased when light intensity was over 30% RI. At low light levels, plants enhanced light availability by means of increasing biomass allocation to leaves and formation of larger, thinner leaves with high specific leaf area （SLA）, leading to a high leaf area ratio （LAR） and high stem strips length （SSL）. The mean relative growth rate （RGR） of the plant increased with the light intensity increase and attained the maximum at 55% RI. The growth of seedlings at 30%-55% RI was much better than that at full light condition. This might be an adaptive strategy that supports the vigorous invasiveness of this species, because a high-shaded canopy could prevent other plant species from surviving and growing. This study indicates that E. adenophorum could adapt to different light conditions, especially to low light habit. This can explain its greater invasiveness.

Evaluation on Killing Effects of Sensitive Plant Growth Inhibitors on Eupatorium adenophorum

[Objective] Considering invasion of Eupatorium adenophorum, a growth in-hibitor of the plant was developed based on plant sensitivity, to make evaluation on control effects and to determine the optimal concentration. [Method] According to field test method, the effects of treatments with growth inhibitor at 0.5%, 1%, 1.5%and 2% on Eupatorium adenophorum were explored and the growth of other weeds was observed to research selectivity of plant inhibitor on the plant. [Result] The growth inhibitor had significant effects on ground parts of Eupatorium adenophorum. Specifical y, after 2 h, Eupatorium adenophorum was damaged seriously and the damage degree went worse upon inhibitor concentration. After 5 d, the control effect of the inhibitorreached 41.5% with concentration at 1.5%, reached 90.2% with the concentration at 1%, and 100% with the concentration at 1.5% and 2%. After 15 d, the control effect achieved 64.6%, 91.7%, 98.9% and 100% with concentrations at 0.5%, 1%, 1.5% and 2%. Stil , the effects of growth inhibitors on root system were limited. For example, new branches would grow from base part if the inhibitor con-centration is too low. On the other hand, the growth inhibitor is of sensitivity and selectivity, which would not hurt other plants. [Conclusion] It is feasible to rapidly control growth and development and even kil Eupatorium adenophorum based on plant sensitivity and it is proved that the growth inhibitor at 1.5% would considerably restrict and kil Eupatorium adenophorum. Therefore, the concentration of growth in-hibitors should be over 1.5%.

Two New Sesquiterpenes from Eupatorium lindleyanum

Two new germacrane sesquiterpenes： 3,8,14-trihydroxy- 1 （10）E,4Z, 11 （13）-germacra- trien-12, 6-olide, 8-（2-methyl-4-hydroxyl-2E-butenoyl）, 3,14-diacetoxy （eupalinolide A, 1）, and 3,8,14-trihydroxy-1（10） E, 4E, 11（13）-germacratrien-12,6-olide, 8-2-methyl-4-hydroxyl-2E- butenoyl）, 3,14-diacetoxy （eupalinolide B, 2） were isolated from Eupatorium lindleyanum. Their structures were elucidated by means of ^1H and ^13C NMR spectroscopic analysis, including 2D NMR technique.

紫茎泽兰(Eupatorium adenophorum)入侵地的生物多样性

生物多样性演变是全球变化的重要组成部分,物种入侵对生物多样的影响已成为当前全球变化领域的研究热点之一。本文研究了西双版纳紫茎泽兰(Eupatorium adenophorum)入侵地的植物、土壤动物与土壤微生物的多样性,结果表明:(1)不同程度入侵区的植物种类及其重要值差别较大,在重度入侵区,紫茎泽兰的重要值高达237.74%,但随着入侵程度的降低,植物种类有所增加且紫茎泽兰的重要值随之降低;丰富度指数、多样性指数、优势度指数与均匀度指数均表现为轻度入侵区>中度入侵区>重度入侵区。(2)三种入侵区的土壤动物隶属23目,其中弹尾目(Collembola)、蜱螨目(Acarina)和膜翅目(Hymenoptera)为优势种群,三者总量占捕获总数的61.77%;丰富度指数、多样性指数、优势度指数、均匀度指数与密度类群指数均表现为轻度入侵区>中度入侵区>重度入侵区。(3)土壤微生物可培养数量类群方面,细菌表现为中度入侵区>重度入侵区>轻度入侵区,放线菌、真菌、自生固氮菌与氨氧化细菌均表现为重度入侵区>中度入侵区>轻度入侵区;多样性指数表现为重度入侵区>轻度入侵区>中度入侵区,优势度指数表现为轻度入侵区=中度入侵区>重度入侵区,均匀度指数表现为重度入侵区>轻度入侵区=中度入侵区。可见,紫茎泽兰入侵地的植物与土壤动物多样性随入侵程度加重而降低,但土壤微生物多样性却随入侵程度加重反而呈上升趋势。今后,加强室内模拟与野外定点长期观测相结合试验是探究紫茎泽兰入侵对生物多样性影响机制的研究方向。

入侵物种飞机草(Eupatorium odoratum)化学成分及生物活性研究进展

就外来入侵恶性杂草飞机草(Eupatorium odoratum)的化学成分及生物活性研究情况进行综述,旨在为治理和综合利用飞机草工作提供参考依据.

Eupatoranolide, a New Sesquiterpene Lactone from Eupatorium adenophorum

A new sesquiterpene lactone eupatoranolide was isolated from the flowers of Eupatorium adenophorum, its structure was elucidated as 2 beta-acetoxy-(7 alpha, 9 beta H)-3.6(11)-cadinadien-12(7)-olide by spectral analysis.

Control Effect of Several Replacement Plants on Invasive Weed Eupatorium adenophorum Spreng

To select the suitable plant species controlling Eupatorium adenophorum in hilly area, ecological adaptability, competitiveness and control efficacy of many replacement plants with economic value in Guizhou Province of China were studied. The results showed that the coverage of paspalum wetsfeteini and Dolichos lablab were 100% and 93%, which increased by 3.70 and 3.44 times compared with Lolium perenne, respectively; their relative crowding coefficient with E. adenophorum were 7.09 and 22.78, which increased by 2.43 and 7.80 times compared with L perenroe, respectively. Using excavation method, the control efficacies of replacement plants were 99.3 % and 96.9%, respectively, while the control efficacy of replacement plants using mowing method was lower than 66.4%. The overwintering rate of P. wetsfeteini in the following year was 95% ; its coverage was still 100% and its control efficacy against E. adenophorum remained over 99%. D. lablab was difficult to survive the winter in the north region beyond 26.2°N, so it could only be applied as annual replacement plant. The coverage of Setaria anceps, Cajanus cajan and other test plants were less than 90%, with poor control efficacy against E. adenophorum.

Toxicity Assessment of Cadinene Sesquiterpenes from Eupatorium adenophorum in Mice

This study evaluated toxic efficacy of Eupatorium adenophorum extracts,against the Kunming mice.In acute study,we firstly tested median lethal dose(LD50)in mice of three cadinene sesquiterpenes 2-deoxo-2-(acetyloxy)-9-oxoageraphorone(DAOA),9-oxo-agerophorone(OA)and 9-oxo-10,11-dehydro-agerophorone(ODA)from Eupatorium adenophorum(Ea).DAOA(215–4640 mg/kg BW,given orally)showed lowest LD50 at 926 mg/kg BW for male mice in contrast with OA(1470 mg/kg BW)and ODA(1470 mg/kg BW).In sub-acute study,repeated doses(75–300 mg/kg BW,for 7 days)of DAOA/OA increased blood parameters,liver and spleen index in dose dependent relationship,along with decrease in thymus index.The blood biochemical and histopathological examination showed that DAOA/OA dose 300 mg/kg BW significantly causes pathological changes of hepatic lobules and hepatocytes,which are consistent with cholestasis and hepatic injury.75 mg/kg dose of DAOA/OA was found to be approximately/totally safe over the span of 7 days treatment showing no change in all above described parameters.Cadinene sesquiterpenes guarantee low risk to environment as a type of low toxic botanical components,which may find potential application in biopesticides development field.

川西南紫茎泽兰(Eupatorium adenophorum)旱季光合生理及其在四川盆地的入侵风险分析

起源于墨西哥的紫茎泽兰是一种入侵我国云南和川西南等地危害极大的杂草.通过在金沙江干热河谷旱季对不同海拔和干旱程度的紫茎泽兰的光合作用进行原位观测和控制条件监测发现,紫茎泽兰光合作用旱季日变化方面具有典型的双峰曲线,光合作用抑制时间为14∶00-15∶00.干旱和高海拔可能提高紫茎泽兰叶片的净光合速率,水分利用效率亦随着干旱和海拔升高而提高,这表明紫茎泽兰对干旱河谷旱季干旱和高温环境的适应.同时,在叶面有效光合辐射(PAR)1,740μmol.m-2.s-1、叶面温度44℃的条件下仍然能够正常光合作用,并具有较高的净光合作用速率,显示出极高的抗高温和高光强胁迫能力,这些生理特点决定了紫茎泽兰比其它植物具有更大的光合作用优势和抗胁迫环境优势,从而决定其在营养生长竞争中对其它大多数植物的比较优势.紫茎泽兰净光合作用对叶面温度和光照强度的响应结果显示,紫茎泽兰光饱和点随着温度的升高而降低,最大净光合作用峰值在25℃至40℃之间,高温对紫茎泽兰光合作用有抑制作用,温度对光照有一定的补偿作用,这暗示紫茎泽兰潜在扩散区域气候特点是冬季具有较高光照或者较高温度或者二者同时具备,低温低光照不利于紫茎泽兰越冬和获得竞争优势.综上所述,紫茎泽兰在四川首要的潜在扩散区域和预防重点是横断山区中段和中北段干旱河谷地区(干暖和干温河谷)、川南宜宾和泸州各区县、以及盆地中部一些光热条件较好的局部区域.

EaIspF1, Essential Enzyme in Isoprenoid Biosynthesis from Eupatorium adenophorum, Reveals a Novel Role in Light Acclimation

Isoprenoids are a functionally and structurally diverse class of natural organic chemicals. The universal precursors of all isoprenoids, isopentenyl diphosphate and dimethylallyl diphosphate are synthesized through the mevalonate and 2C-methyl- D-erythritol 4-phosphate （MEP） pathways, respectively. Many isoprenoids produced through the MEP pathway play an important role in plant acclimation to different light environments. Eupatorium adenophorum, an invasive weed in China, presents a remarkable capacity to acclimate to various light environments, which constitutes its solid foundation of being a successful invasive species. Thus we aimed at gaining a deeper insight into the regulation of MEP pathway in E. adenophorum to further understand the invasive mechanism. 2C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase （IspF or MCS） is an essential enzyme in the MEP pathway. In this paper, a novel IspF gene was cloned and characterized from E. adenophorum. Tissue-specific expression assays revealed a higher expression of EalspF1 in leaves than in stems and roots. The expression of EalspF1 was responsive to different light conditions. Some up-regulation of EalspF1 expression was also found after the treatments with signal compounds and after wounding stress. Interestingly, the over-expression of EalspF1 in Arabidopsis led to increase carotenoids contents, resulting in an enhanced tolerance to high light. Taken together, these results indicate that the EalspFl-derived enzyme participates in isoprenoid metabolism and among others, the expression of this gene in E. adenophorum is involved in the regulation of plastidial isoprenoids, which play an important role in acclimation to various light environments.

Highly Oxygenated Monoterpenes from Eupatorium fortunei

A pair of epimers of highly-oxygenated monoterpenes were isolated from the traditional Chinese medicine Eupatorium fortunei. Their structures were elucidated on the basis of the spectral analysis as （1R＊, 2S＊, 3R＊, 4R＊, 6S＊）-1, 2, 3, 6-tetrehydroxy-p-menthane （1） and （1S＊, 2S＊, 3S＊, 4R＊, 6R＊）-1, 2, 3, 6-tetrehydroxy-p-menthane （2）.

Habitat Factor Analysis of Alien Invasive Plant Eupatorium odoratum in Guangxi Province of China

The invasion pattern of Eupatorium odoratum in invasion area was studied by random plot sampling method. Eighteen habitat factors of E. odoratum sampling plots such as altitude, gradient and exposure were quantified to precisely describe the invasion patterns of E. odoratum in various habitats with different characteristics, and carried out principal component analysis （PCA）. The results showed that the ecological information of 18 habitat factors of E. odoratum could be characterized by four comprehensive factors. The occurrence frequency of E. odoratum had positive eorrelation with the ecological factors such as exposure, soil moisture, total number of plants, number of shrubs, weeds height, distance form water source, farmland distance, air humidity, arbor height, arbor size, altitude, gradient, number of plant species and weeds coverage, which had negative correlation with air temperature.

Individual-based simulation of the diffusion process of Eupatorium adenophorum

In order to investigate the diffusion patterns of Eupatorium adenophorum, this study uses a computer to simulate various plant diffusion processes under different environments. The study develops binary cellular automata as a framework reflecting the growth and semination of plants by the change of cellular state and transition function. This model allocates 16 integers, namely 1 to 16, to indicate a life history of plant starting from seed. In each iteration （a year）, Monte-Carlo simulation is applied to decide whether an individual survives into the next year, the probability of which is estimated from previous literature. If a plant survives youth, mature or senile period, its propagation will also be considered and short-tailed dispersal kernel was employed. Applying this basic model, this study also simulates the diffusion process of E. adenophorum under four typical ecological environments. Examining the age structure of the four populations, we found that, although with significantly different numbers of plants left, most individuals were in the infancy period. Besides, their dispersal velocity showed different periodic law. These observations provided reasonable suggestions on what control strategies to be taken respectively.

Control Effect of Replacement Cultivation of Several High Quality Forages against Eupatorium adenophorum

[Objective] The paper was to analyze the control effect of replacement cultivation of several high quality forages against Eupatorium adenophorum. [ Method] Five high quality forages including Lolium perenne, Dactylis glomerata, Setaria anceps, Trifolium repens and Medicago sativa were planted in the fields invaded by E. adenophorum, and their ecological adaptability and control effect against E. adenophorum were studied. [Result] Five kinds of forages could nor- mally grow at the local land. The indexes such as coverage, plant height, fresh weight of stems and leaves of S. anceps were the highest, and it showed very strong ecological adaptability as well as the best control effect on E. adenophorum, up to 97.85% and 93.81% at the first and second year, respectively, indicating good sustainable control effect. D. glomerata ranked the second place, of which the coverage after one year was 86% and the control effect on E. adenophorum was 89.68%. The other species showed poor control effect on E. adenophorum because of slow growth rate and heavily damage from companion weeds even they could establish dominant communities. [ Condusion] The paper provided reference for ecological control against E. adenophorum in local land and ecological restoration of invasive land.

Technology in Utilization of Eupatorium adenophorum Biomass Energy

A simple, easily-operated and economical carbonization technology with Eupatorium adenophorum was introduced in terms of instrument selection, carboniza- tion of Eupatorium adenophorum and preparation of carbon rod, and evaluation was made on economic profits from carbonization of Eupatorium adenophorum.

紫茎泽兰Eupatorium adenophorum Spreng.在中国入侵分布预测

原产于墨西哥的紫茎泽兰 Eupatorium adenophorum Spreng.作为一个有害的外来物种在印度、新西兰和澳大利亚生长已有很长时间。在中国 ,尤其是在南方和西南地区其蔓延速度之快 ,带来了不可忽视的经济和社会后果。我们采用了生态位模拟新方法来预测紫茎泽兰可能入侵的范围。据此 ,预测的潜在分布区包括该植物在中国境内已分布的省份及未来华中。

Differential Responses of the Activities of Antioxidant Enzymes to Thermal Stresses between Two Invasive Eupatorium Species in China

The effect of thermal stress on the antioxidant system was investigated in two invasive plants, Eupatorium adenophorum Spreng. and E. odoratum L. The former is sensitive to high temperature, whereas the latter is sensitive to low temperature. Our aim was to explore the relationship between the response of antioxidant enzymes and temperature in the two invasive weeds with different distribution patterns in China. Plants were transferred from glasshouse to growth chambers at a constant 25 ℃for I week to acclimatize to the environment. For the heat treatments, temperature was increased stepwise to 30, 35, 38 and finally to 42 ℃. For the cold treatments, temperature was decreased stepwise to 20, 15, 10 and finally to 5 ℃. Plants were kept in the growth chambers for 24 h at each temperature step. In E. adenophorum, the coordinated increase of the activities of antioxidant enzymes was effective in protecting the plant from the accumulation of active oxygen species （AOS） at low temperature, but the activities of catalase （CAT）, guaiacol peroxidase （POD）, ascorbate peroxidase （APX）, glutathione reductase （GR）, and monodehydroascorbate reductase （MDAR） were not accompanied by the increase of superoxide dismutase （SOD） during the heat treatments. As a result, the level of lipid peroxidation in E. adenophorum was higher under heat stress than under cold stress. In E. odoratum, however, the lesser degree of membrane damage, as indicated by low monodehydroascorbate content, and the coordinated increase of the oxygen. Datoxifying enzymes were observed in heat-treated plants, but the antioxidant enzymes were unable to operate in cold stress. This indicates that the plants have a higher capacity for scavenging oxygen radicals in heat stress than in cold stress. The different responses of antioxidant enzymes may be one of the possible mechanisms of the differences in temperature sensitivities of the two plant species.

佩兰治疫浅析

在古今的疫病治疗中,中医药发挥了极为重要的作用,其中芳香化湿类药物在疫病的治疗中受到了历代医家的重视。据古籍研究及文献整理结果显示,佩兰是治疗疫病的高频药物,其芳香走窜之性可宣化清阳,辛轻扬上之性则能畅通气机,具有化湿辟疫、开窍醒神、醒脾开胃等功效。通过系统梳理佩兰的药性、方剂配伍规律、医论基础、药理研究等,探讨其治疫机制,推动佩兰的研究与开发,为临床应用佩兰治疫提供参考依据,丰富疫病治疗手段。