Objective] This study aimed to investigate the relationship between pistil-late flower developmental morphology and stigma receptivity in Euphorbia pulcherri-ma. [Method] One-year-old adult plants of E. pulcherrima cu...Objective] This study aimed to investigate the relationship between pistil-late flower developmental morphology and stigma receptivity in Euphorbia pulcherri-ma. [Method] One-year-old adult plants of E. pulcherrima cultivar ‘Mil enium’ were used as experimental materials to analyze pistil ate flower development process by macroscopic and scanning electron microscope （SEM） observation. Stigma receptivity was detected with benzidine-hydrogen peroxide method and in vivo pol en germina-tion method. [Result] The pistil ate flower development process of E. pulcherrima was consisted of columnar stigma phase, stigma lobe slightly opening phase, Y-shape stigma lobe phase, inverse V-shaped stigma lobe phase and stigma curling phase. Pistil ate flower development and stigma receptivity of E. pulcherrima exhibit-ed certain correlation. The stigma receptive period lasted from stigma lobe slightly opening phase to inverse V-shaped stigma lobe phase; inverse V-shaped stigma lobe phase was appropriate for pol ination, lasting for 3-5 d, with V-shaped stigma lobe and a large amount of exudates on stigma surface according to SEM obser-vation. Stigma receptivity of E. pulcherrima detected with benzidine-hydrogen perox-ide method was consistent with that detected in vivo pol en germination method. [Conclusion] ln breeding practice, the optimal pol ination period of E. pulcherrima can be determined based on the developmental morphology of pistil ate flower.展开更多
By the observation of chloroplast ultrastructure in different period of bract colors of Euphorbia pulcherrima, the paper studied the change of chloroplast ultrastructural in the transition process of bract colors, ide...By the observation of chloroplast ultrastructure in different period of bract colors of Euphorbia pulcherrima, the paper studied the change of chloroplast ultrastructural in the transition process of bract colors, identified the relationship between E.pulcherrima color change and the chloroplast ultrastructure to provide theorical bases for the cultivation management and further study of E. pulcherrima. Ultrastructural study showed that in the process of change from green to red, the chloroplast of bracts disintegrated gradually, lamellar structure was destroyed gradually, and the content of chloroplasts in mesophyll cells was also reduced gradually. When bracts color resumed to turn green gradually, the content of chloroplasts in mesophyll cells was also increased gradually.展开更多
The microsporogenesis and male gametophyte development of Euphorbia pulcherrima were studied with paraffin section method. The results showed that the anther of Euphorbia pulcherrima had four chambers. The development...The microsporogenesis and male gametophyte development of Euphorbia pulcherrima were studied with paraffin section method. The results showed that the anther of Euphorbia pulcherrima had four chambers. The development of anther wall was dicotyledonous type; its wall consisted of epidermis, endothecium, middle layer and tapetum. The mature male gametophyte development experienced seven major stages as the followings： archesporial cells, aporgenous cells, microspore mother cells, tetrads of microspores （tetrahedral）, uninucleate microspores, 2-uncleate pollens and 2-celled pollen stage. The anther wall varied with the pollen development： the epidermis of anther ruptures at the anther mature stage; the middle layer disappears at tetrad stage; the endothelium became fibrous layer at the anther mature stage; the tapetum （glandular） was the most development at the microspore mother cells stage and disappeared at anther mature stage. The abnormal anther development was observed simultaneously.展开更多
以辐热积(每日温度相对热效应×光合有效辐射,Phototherm al product,PTP)为光温指标,通过不同扦插期的试验研究了温度和辐射与‘千禧’一品红发育和品质指标的定量关系,明确了从短日至单苞期、单蕾期、多蕾期、开花期所需要的PTP...以辐热积(每日温度相对热效应×光合有效辐射,Phototherm al product,PTP)为光温指标,通过不同扦插期的试验研究了温度和辐射与‘千禧’一品红发育和品质指标的定量关系,明确了从短日至单苞期、单蕾期、多蕾期、开花期所需要的PTP。结果表明标准化株高净增量(株高净增量/扦插苗初始叶面积)与PTP呈幂函数关系;主干叶片净增数、侧枝叶片数、苞片数与PTP均呈线性关系;冠高比与PTP呈负指数关系。展开更多
基金Supported by Fund for Independent Innovation of Agricultural Science and Technologyin Jiangsu Province[CX(10)411]~~
文摘Objective] This study aimed to investigate the relationship between pistil-late flower developmental morphology and stigma receptivity in Euphorbia pulcherri-ma. [Method] One-year-old adult plants of E. pulcherrima cultivar ‘Mil enium’ were used as experimental materials to analyze pistil ate flower development process by macroscopic and scanning electron microscope （SEM） observation. Stigma receptivity was detected with benzidine-hydrogen peroxide method and in vivo pol en germina-tion method. [Result] The pistil ate flower development process of E. pulcherrima was consisted of columnar stigma phase, stigma lobe slightly opening phase, Y-shape stigma lobe phase, inverse V-shaped stigma lobe phase and stigma curling phase. Pistil ate flower development and stigma receptivity of E. pulcherrima exhibit-ed certain correlation. The stigma receptive period lasted from stigma lobe slightly opening phase to inverse V-shaped stigma lobe phase; inverse V-shaped stigma lobe phase was appropriate for pol ination, lasting for 3-5 d, with V-shaped stigma lobe and a large amount of exudates on stigma surface according to SEM obser-vation. Stigma receptivity of E. pulcherrima detected with benzidine-hydrogen perox-ide method was consistent with that detected in vivo pol en germination method. [Conclusion] ln breeding practice, the optimal pol ination period of E. pulcherrima can be determined based on the developmental morphology of pistil ate flower.
文摘By the observation of chloroplast ultrastructure in different period of bract colors of Euphorbia pulcherrima, the paper studied the change of chloroplast ultrastructural in the transition process of bract colors, identified the relationship between E.pulcherrima color change and the chloroplast ultrastructure to provide theorical bases for the cultivation management and further study of E. pulcherrima. Ultrastructural study showed that in the process of change from green to red, the chloroplast of bracts disintegrated gradually, lamellar structure was destroyed gradually, and the content of chloroplasts in mesophyll cells was also reduced gradually. When bracts color resumed to turn green gradually, the content of chloroplasts in mesophyll cells was also increased gradually.
文摘The microsporogenesis and male gametophyte development of Euphorbia pulcherrima were studied with paraffin section method. The results showed that the anther of Euphorbia pulcherrima had four chambers. The development of anther wall was dicotyledonous type; its wall consisted of epidermis, endothecium, middle layer and tapetum. The mature male gametophyte development experienced seven major stages as the followings： archesporial cells, aporgenous cells, microspore mother cells, tetrads of microspores （tetrahedral）, uninucleate microspores, 2-uncleate pollens and 2-celled pollen stage. The anther wall varied with the pollen development： the epidermis of anther ruptures at the anther mature stage; the middle layer disappears at tetrad stage; the endothelium became fibrous layer at the anther mature stage; the tapetum （glandular） was the most development at the microspore mother cells stage and disappeared at anther mature stage. The abnormal anther development was observed simultaneously.
文摘以辐热积(每日温度相对热效应×光合有效辐射,Phototherm al product,PTP)为光温指标,通过不同扦插期的试验研究了温度和辐射与‘千禧’一品红发育和品质指标的定量关系,明确了从短日至单苞期、单蕾期、多蕾期、开花期所需要的PTP。结果表明标准化株高净增量(株高净增量/扦插苗初始叶面积)与PTP呈幂函数关系;主干叶片净增数、侧枝叶片数、苞片数与PTP均呈线性关系;冠高比与PTP呈负指数关系。
