1 mL of sporangial suspensions (5 x 10 5spporangia per milliliter) of Pseudoperonospora cubensis was droplet-inoculated on the surface of the second leaf of the plant grown in greenhouse ( inducing inoculatoin), then ...1 mL of sporangial suspensions (5 x 10 5spporangia per milliliter) of Pseudoperonospora cubensis was droplet-inoculated on the surface of the second leaf of the plant grown in greenhouse ( inducing inoculatoin), then the lower surfaces of the third, the forth and the fifth leaves were uniformly sprayed with inoculum of the same fungi (5 x 10 4sporangia per milliliter, about 5 mL per plant) every 3 days interval (challenge inoculation). Plants were moistened at 18- 22 C for 18 h, then kept at room temperature (24 - 28 C) and supplemented with cool-white fluorescent lights. All three challenge leaves were collected after 7 days of challenge to measure the amount of sporulation and area of necrosis. Plants prior inoculated with P. cubensis were protected 38% (based on the area of necrosis) against disaesc caused by subsequent foliar challenge with the pathogen. Protective action was about 12% after 3 days, and maintained this level until 9 days, suddenly reached 34% after 12 days, and came to a maximum after 15 days, then dropped down slowly.展开更多
文摘1 mL of sporangial suspensions (5 x 10 5spporangia per milliliter) of Pseudoperonospora cubensis was droplet-inoculated on the surface of the second leaf of the plant grown in greenhouse ( inducing inoculatoin), then the lower surfaces of the third, the forth and the fifth leaves were uniformly sprayed with inoculum of the same fungi (5 x 10 4sporangia per milliliter, about 5 mL per plant) every 3 days interval (challenge inoculation). Plants were moistened at 18- 22 C for 18 h, then kept at room temperature (24 - 28 C) and supplemented with cool-white fluorescent lights. All three challenge leaves were collected after 7 days of challenge to measure the amount of sporulation and area of necrosis. Plants prior inoculated with P. cubensis were protected 38% (based on the area of necrosis) against disaesc caused by subsequent foliar challenge with the pathogen. Protective action was about 12% after 3 days, and maintained this level until 9 days, suddenly reached 34% after 12 days, and came to a maximum after 15 days, then dropped down slowly.
