Accurate diagnosis of different bronchopul- monary diseases is important in clinical practice. This study involved 20 healthy volunteers and 77 patients with bronchopulmonary diseases, including chronic obstructive pu...Accurate diagnosis of different bronchopul- monary diseases is important in clinical practice. This study involved 20 healthy volunteers and 77 patients with bronchopulmonary diseases, including chronic obstructive pulmonary disease （COPD）, bronchial asthma, pulmonary tuberculosis, and community-acquired pneumonia. The absorption spectrum of exhaled air samples was recorded on an intra-cavity photo-acoustic gas analyzer （ILPA-1, Special Technologies, Ltd., Russia） with photo-acoustic detectors and CO2 laser with a tuning range from 9.2 to 10.8μm. In conclusion, analysis of the Mahalanobis distance-based absorption spectral profiles of breath air from bronchopulmonary patients and healthy volunteers allows the formulation of a preliminary diagnosis.展开更多
Direct determination of the oxidative potential in exhaled air(OPEA)as a biological indicator of the redox balance status in airways requires an extremely sensitive sensing approach to counterbalance the high dilution...Direct determination of the oxidative potential in exhaled air(OPEA)as a biological indicator of the redox balance status in airways requires an extremely sensitive sensing approach to counterbalance the high dilution of the exhaled air matrix.In opposite to standard colorimetric assays based on Beer-Lambert Law,the use of simple multiscattering-enhanced absorbance(MEA)strategy results in an improved photonic sensing system.Based on this strategy and using the ferrous-xylenol orange assay,analytical performances allowing the detection of 3 pmol of H_(2)O_(2) could be obtained.The comparative analysis of photometric configurations–standard vs MEA–highlights the large absorbance gain obtained for tiny H_(2)O_(2) amounts–from 0 to 30 pmol–whereas similar sensing trends are observed at higher concentration range.The automated introduction of exhaled air sample into the reaction compartment via needle-impinger coupled with the MEA-based photonic cell enables ultrasensitive determination of OPEA in short time(<5 min).A pilot study conducted on healthy subjects(n=22)and chronic obstructive pulmonary disease(COPD)patients(n=23)volunteers shows a significant increase of the OPEA for COPD versus controls and strong negative correlation between OPEA and spirometry parameters(Tiffeneau index:FEV1/FVC)for ex-/non-smokers.Application perspectives to assess inflammatory episodes in obstructive lung diseases are thus made possible using this sensing approach.展开更多
文摘Accurate diagnosis of different bronchopul- monary diseases is important in clinical practice. This study involved 20 healthy volunteers and 77 patients with bronchopulmonary diseases, including chronic obstructive pulmonary disease （COPD）, bronchial asthma, pulmonary tuberculosis, and community-acquired pneumonia. The absorption spectrum of exhaled air samples was recorded on an intra-cavity photo-acoustic gas analyzer （ILPA-1, Special Technologies, Ltd., Russia） with photo-acoustic detectors and CO2 laser with a tuning range from 9.2 to 10.8μm. In conclusion, analysis of the Mahalanobis distance-based absorption spectral profiles of breath air from bronchopulmonary patients and healthy volunteers allows the formulation of a preliminary diagnosis.
基金This study was supported in the frame of the project OxAirDirect(Grant EST2015-1-151)funded by the French Agency for Food,Environmental and Occupational Health&Safety(ANSES)the project ROBoCoP(Grant IZCOZ0-177,067)funded by the Swiss National Science Foundation(SNSF)the framework of CliniMARK COST Action(CA16113).
文摘Direct determination of the oxidative potential in exhaled air(OPEA)as a biological indicator of the redox balance status in airways requires an extremely sensitive sensing approach to counterbalance the high dilution of the exhaled air matrix.In opposite to standard colorimetric assays based on Beer-Lambert Law,the use of simple multiscattering-enhanced absorbance(MEA)strategy results in an improved photonic sensing system.Based on this strategy and using the ferrous-xylenol orange assay,analytical performances allowing the detection of 3 pmol of H_(2)O_(2) could be obtained.The comparative analysis of photometric configurations–standard vs MEA–highlights the large absorbance gain obtained for tiny H_(2)O_(2) amounts–from 0 to 30 pmol–whereas similar sensing trends are observed at higher concentration range.The automated introduction of exhaled air sample into the reaction compartment via needle-impinger coupled with the MEA-based photonic cell enables ultrasensitive determination of OPEA in short time(<5 min).A pilot study conducted on healthy subjects(n=22)and chronic obstructive pulmonary disease(COPD)patients(n=23)volunteers shows a significant increase of the OPEA for COPD versus controls and strong negative correlation between OPEA and spirometry parameters(Tiffeneau index:FEV1/FVC)for ex-/non-smokers.Application perspectives to assess inflammatory episodes in obstructive lung diseases are thus made possible using this sensing approach.
