The paper aimed to study the effects of exogenous hormone on fecundity of silver fox. Vixens were timely injected with appropriate exogenous hormones at proper ratio to increase their ovulation and litter size, thereb...The paper aimed to study the effects of exogenous hormone on fecundity of silver fox. Vixens were timely injected with appropriate exogenous hormones at proper ratio to increase their ovulation and litter size, thereby improving the fecundity of silver fox. The average litter size in experimental group attained by 5.86 to 6.0 whelps, 1.61 - 1.75 whelps more than that in the control group. The hormone combination in the experimental group was high in performance/price ratio, which had no significant impact on fecundity of vixens in future.展开更多
Objectives] This study was conducted to investigate the regulatory mechanism of ethylene on DpXTH gene expression in dahlia ( Dahlia pinnata Cav.) flower petals.[Methods] Dahlia flower petals were treated with ethep...Objectives] This study was conducted to investigate the regulatory mechanism of ethylene on DpXTH gene expression in dahlia ( Dahlia pinnata Cav.) flower petals.[Methods] Dahlia flower petals were treated with ethephon (ETH) and 1-methylcyclopropene (1-MCP) at different flower development stages and for different durations in vivo . Then, the relative expression of DpXTH1 and DpXTH2 in flower samples in different treatments was quantified with real-time PCR, using β-actin as a reference gene.[Results] The expression of DpXTH1 and DpXTH2 in ETH- or 1-MCP-treated dahlia flower petals increased at first, and decreased subsequently with increasing duration of treatment. The relative expression of both DpXTH1 and DpXTH2 in ETH-treated samples reached the maximum level at 12 h. Moreover, the relative expression of both DpXTH1 and DpXTH2 in dahlia flower petals treated with ETH at early stage of flowering was higher than that of the samples treated at other flowering stages; in 1-MCP-treated samples, it was higher at full-flowering stage than at other stages. The maximum relative expression levels of DpXTH1 and DpXTH2 in ETH- and 1-MCP-treated samples were all higher than those of control.[Conclusions] Exogenous ethylene can accelerate the opening of dahlia flowers, and 1-MCP can inhibit this process, so they can be used to regulate development of dahlia flowers.展开更多
The rooting capacity of Pinus massoniana is poor,especially for mature trees,and has prevented the development of clonal forestry for P.massoniana.In this study,we varied explant types,subculture times and exogenous h...The rooting capacity of Pinus massoniana is poor,especially for mature trees,and has prevented the development of clonal forestry for P.massoniana.In this study,we varied explant types,subculture times and exogenous hormones for plantlet regeneration and assessed shoots for rooting rate and root number for P.massoniana.Following fi ve repetitive grafts,new shoots from grafts used as explant sources were rejuvenated as observed from juvenile shoot morphology and anatomy,leading to greatly enhanced plant regeneration in comparison to that of mature materials from 26-year-old P.massoniana trees.The rooting capacity of subcultured shoots increased with successive subcultures,reaching a peak at 20 subcultures with 35–40 days per subculture.However,rooting performance was signifi cantly reduced after 30 subcultures.The addition of naphthaleneacetic acid(NAA)plus indoleacetic acid in the medium improved the root number,but the combination of exogenous NAA with paclobutrazol(PBZ)increased rooting rate and root number.We thus greatly improved the rooting capacity of mature P.massoniana trees by optimizing explant types(rejuvenated),subculture times(20 subcultures,35–40 days per subculture)and addition of NAA+PBZ to the rooting medium.The conditions can be used for effi cient plantlet regeneration of P.massoniana.展开更多
文摘The paper aimed to study the effects of exogenous hormone on fecundity of silver fox. Vixens were timely injected with appropriate exogenous hormones at proper ratio to increase their ovulation and litter size, thereby improving the fecundity of silver fox. The average litter size in experimental group attained by 5.86 to 6.0 whelps, 1.61 - 1.75 whelps more than that in the control group. The hormone combination in the experimental group was high in performance/price ratio, which had no significant impact on fecundity of vixens in future.
基金Supported by Applied Basic Research Project of Suzhou City(SYN201405)
文摘Objectives] This study was conducted to investigate the regulatory mechanism of ethylene on DpXTH gene expression in dahlia ( Dahlia pinnata Cav.) flower petals.[Methods] Dahlia flower petals were treated with ethephon (ETH) and 1-methylcyclopropene (1-MCP) at different flower development stages and for different durations in vivo . Then, the relative expression of DpXTH1 and DpXTH2 in flower samples in different treatments was quantified with real-time PCR, using β-actin as a reference gene.[Results] The expression of DpXTH1 and DpXTH2 in ETH- or 1-MCP-treated dahlia flower petals increased at first, and decreased subsequently with increasing duration of treatment. The relative expression of both DpXTH1 and DpXTH2 in ETH-treated samples reached the maximum level at 12 h. Moreover, the relative expression of both DpXTH1 and DpXTH2 in dahlia flower petals treated with ETH at early stage of flowering was higher than that of the samples treated at other flowering stages; in 1-MCP-treated samples, it was higher at full-flowering stage than at other stages. The maximum relative expression levels of DpXTH1 and DpXTH2 in ETH- and 1-MCP-treated samples were all higher than those of control.[Conclusions] Exogenous ethylene can accelerate the opening of dahlia flowers, and 1-MCP can inhibit this process, so they can be used to regulate development of dahlia flowers.
基金the Project of Scientifi c and Technological Plan from the Department of Science and Technology of Guangxi under Grants 2018GXNSFDA281020,AD17195078,2017GXNSFAA198037 and AA17204087-1the Natural Science Foundation of China under Grant 31960311 and 31360178the Key Program of Guangxi Forestry Bureau under Grant[2016]13 and GL2019KT06.
文摘The rooting capacity of Pinus massoniana is poor,especially for mature trees,and has prevented the development of clonal forestry for P.massoniana.In this study,we varied explant types,subculture times and exogenous hormones for plantlet regeneration and assessed shoots for rooting rate and root number for P.massoniana.Following fi ve repetitive grafts,new shoots from grafts used as explant sources were rejuvenated as observed from juvenile shoot morphology and anatomy,leading to greatly enhanced plant regeneration in comparison to that of mature materials from 26-year-old P.massoniana trees.The rooting capacity of subcultured shoots increased with successive subcultures,reaching a peak at 20 subcultures with 35–40 days per subculture.However,rooting performance was signifi cantly reduced after 30 subcultures.The addition of naphthaleneacetic acid(NAA)plus indoleacetic acid in the medium improved the root number,but the combination of exogenous NAA with paclobutrazol(PBZ)increased rooting rate and root number.We thus greatly improved the rooting capacity of mature P.massoniana trees by optimizing explant types(rejuvenated),subculture times(20 subcultures,35–40 days per subculture)and addition of NAA+PBZ to the rooting medium.The conditions can be used for effi cient plantlet regeneration of P.massoniana.