Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified ...Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified probe （TPE-Py-DNA） as a fluorescence reporter and exonuclease llI （Exo lIl） as a signal amplifier. With the aid of telomerase, repeat units （TrAGGG）n are extended from the end of template substrate oligonucleotides （TS primer） that form duplex DNAs with TPE-Py-DNA. Then, Exo llI catalyzes the diges- tion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal. The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally, we obtain the target-to-signal amplification ratio of 1 ：N2. This strategy exhibits good performance for detecting clinical urine samples （distinguishing 15 cancer patients＇ samples from 8 healthy ones） and checking intracellular telomerase activity （differentiating cell lines including HeLa, MDA-MB-231, MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug）, which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer.展开更多
The detection of biomarkers is of great significance in the diagnosis of numerous diseases,especially cancer.Herein,we developed a sensitive and universal fluorescent aptasensor strategy based on magnetic beads,DNA G-...The detection of biomarkers is of great significance in the diagnosis of numerous diseases,especially cancer.Herein,we developed a sensitive and universal fluorescent aptasensor strategy based on magnetic beads,DNA G-quadruplex,and exonuclease Ⅲ(Exo Ⅲ).In the presence of a target protein,a label-free single strand DNA(ssDNA)hybridized with the aptamer was released as a trigger DNA due to specific recognition between the aptamer and target.Subsequently,ssDNA initiates the ExoⅢ-aided recycling to amplify the fluorescence signal,which was caused by N-methylmesoporphyrin IX(NMM)insertion into the G-quadruplex structure.This proposed strategy combines the excellent specificity between the aptamer and target,high sensitivity of the fluorescence signal by G-quadruplex and ExoⅢ-aided recycling amplification.We selected(50-1200 nmol/L)MUC1,a common tumor biomarker,as the proof-of-concept target to test the specificity of our aptasenso r.Results reveal that the sensor sensitively and selectively detected the target protein with limits of detection(LODs)of 3.68 and 12.83 nmol/L in buffer solution and 10%serum system,respectively.The strategy can be easily applied to other targets by simply substituting corresponding aptamers and has great potential in the diagnosis and monitoring of several diseases.展开更多
Mercury ion(Hg^(2+)),a highly noxious of heavy metalion,has detrimental effects on the ecological environment and human health.Herein,we have developed an exonuclease III(Exo III)assisted catalytic hairpin assembly fo...Mercury ion(Hg^(2+)),a highly noxious of heavy metalion,has detrimental effects on the ecological environment and human health.Herein,we have developed an exonuclease III(Exo III)assisted catalytic hairpin assembly formation of a trivalent G-quadruplex/hemin DNAzyme for colorimetric detection of Hg^(2+).A hairpin DNA(Hr)was designed with thymine-Hg^(2+)-thymine pairs that catalyzed by Exo III is prompted to happen upon binding Hg^(2+).A released DNA fragment triggers the catalytic assembly of other three hairpins(H1,H2,and H3)to form many trivalent G-quadruplex/hemin DNA enzymes for signal output.The developed sensor shows a dynamic range from 2 pM to 2μM,with an impressively low detection limit of 0.32 pM for Hg^(2+)detection.Such a sensor also has good selectivity toward Hg^(2+)detection in the presence of other common metal ions.This strategy shows the great potential for visual detection with portable type.展开更多
目的对比改良肩关节镜下双纽扣钢板动态固定与锁骨钩钢板切开复位内固定治疗RockwoodⅢ~Ⅴ型肩锁关节脱位的中期疗效。方法回顾性分析2015年1月至2019年12月华中科技大学同济医学院附属同济医院收治的51例RockwoodⅢ~Ⅴ型肩锁关节脱位...目的对比改良肩关节镜下双纽扣钢板动态固定与锁骨钩钢板切开复位内固定治疗RockwoodⅢ~Ⅴ型肩锁关节脱位的中期疗效。方法回顾性分析2015年1月至2019年12月华中科技大学同济医学院附属同济医院收治的51例RockwoodⅢ~Ⅴ型肩锁关节脱位患者资料,其中RockwoodⅢ型19例,Ⅳ型18例,Ⅴ型14例;男32例,女19例;年龄11~65岁,平均(38.6±17.2)岁。21例行改良肩关节镜下双纽扣钢板动态固定术(A组),30例行锁骨钩钢板切开复位内固定术(B组)。比较两组术中及术后住院情况,采用Constant-Murley评分、加利福尼亚大学洛杉矶分校(University of California at Los Angeles,UCLA)评分、疼痛视觉模拟评分(visual analogue scale,VAS)评估肩关节功能。结果患者术后随访24~29个月,平均(24.4±5.2)个月。相较于B组,A组术中出血量更少,住院时间更短,住院总费用更低,但手术时间更长,差异均有统计学意义(P<0.05)。术后3周、6周、12周,A组的Constant-Murley评分、UCLA评分和VAS均优于B组(P<0.05);末次随访时以上3项评价指标组间比较差异均无统计学意义(P>0.05)。两组Constant-Murley评分和UCLA评分术后生存率比较,术后早期A组明显优于B组,末次随访时两组差异无统计学意义。结论对于RockwoodⅢ~Ⅴ型肩锁关节脱位,术后早期(术后3周、6周、12周)关节镜下改良纽扣钢板动态固定更有优势,术后中期(术后24个月)两种方式的治疗效果差异无统计学意义。展开更多
基金supported by the National Natural Science Foundation of China(21375042,21405054,21525523,21574048,and21404028)the National Basic Research Program of China(2015CB932600,2013CB933000,and 2016YFF0100800)+1 种基金the Special Fund for Strategic New Industry Development of Shenzhen,China(JCYJ20150616144425376)1000 Young Talent Program(to F.Xia)
文摘Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified probe （TPE-Py-DNA） as a fluorescence reporter and exonuclease llI （Exo lIl） as a signal amplifier. With the aid of telomerase, repeat units （TrAGGG）n are extended from the end of template substrate oligonucleotides （TS primer） that form duplex DNAs with TPE-Py-DNA. Then, Exo llI catalyzes the diges- tion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal. The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally, we obtain the target-to-signal amplification ratio of 1 ：N2. This strategy exhibits good performance for detecting clinical urine samples （distinguishing 15 cancer patients＇ samples from 8 healthy ones） and checking intracellular telomerase activity （differentiating cell lines including HeLa, MDA-MB-231, MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug）, which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer.
基金supported by grants from the National Natural Science Foundation of China (No.21472060)Shenzhen Municipal government (Nos.JCYJ20160301153959476 and JCYJ20160324163734374)Shenzhen Reform Commission (Disciplinary Development Program for Chemical Biology)
文摘The detection of biomarkers is of great significance in the diagnosis of numerous diseases,especially cancer.Herein,we developed a sensitive and universal fluorescent aptasensor strategy based on magnetic beads,DNA G-quadruplex,and exonuclease Ⅲ(Exo Ⅲ).In the presence of a target protein,a label-free single strand DNA(ssDNA)hybridized with the aptamer was released as a trigger DNA due to specific recognition between the aptamer and target.Subsequently,ssDNA initiates the ExoⅢ-aided recycling to amplify the fluorescence signal,which was caused by N-methylmesoporphyrin IX(NMM)insertion into the G-quadruplex structure.This proposed strategy combines the excellent specificity between the aptamer and target,high sensitivity of the fluorescence signal by G-quadruplex and ExoⅢ-aided recycling amplification.We selected(50-1200 nmol/L)MUC1,a common tumor biomarker,as the proof-of-concept target to test the specificity of our aptasenso r.Results reveal that the sensor sensitively and selectively detected the target protein with limits of detection(LODs)of 3.68 and 12.83 nmol/L in buffer solution and 10%serum system,respectively.The strategy can be easily applied to other targets by simply substituting corresponding aptamers and has great potential in the diagnosis and monitoring of several diseases.
基金Supported by The Science and Technology Project of General Administration of Quality Supervision,Inspection and Quarantine (2015IK126)The Science and Technology Project of Changsha City of Hunan Province of China (KQ1602124).
文摘Mercury ion(Hg^(2+)),a highly noxious of heavy metalion,has detrimental effects on the ecological environment and human health.Herein,we have developed an exonuclease III(Exo III)assisted catalytic hairpin assembly formation of a trivalent G-quadruplex/hemin DNAzyme for colorimetric detection of Hg^(2+).A hairpin DNA(Hr)was designed with thymine-Hg^(2+)-thymine pairs that catalyzed by Exo III is prompted to happen upon binding Hg^(2+).A released DNA fragment triggers the catalytic assembly of other three hairpins(H1,H2,and H3)to form many trivalent G-quadruplex/hemin DNA enzymes for signal output.The developed sensor shows a dynamic range from 2 pM to 2μM,with an impressively low detection limit of 0.32 pM for Hg^(2+)detection.Such a sensor also has good selectivity toward Hg^(2+)detection in the presence of other common metal ions.This strategy shows the great potential for visual detection with portable type.
文摘目的对比改良肩关节镜下双纽扣钢板动态固定与锁骨钩钢板切开复位内固定治疗RockwoodⅢ~Ⅴ型肩锁关节脱位的中期疗效。方法回顾性分析2015年1月至2019年12月华中科技大学同济医学院附属同济医院收治的51例RockwoodⅢ~Ⅴ型肩锁关节脱位患者资料,其中RockwoodⅢ型19例,Ⅳ型18例,Ⅴ型14例;男32例,女19例;年龄11~65岁,平均(38.6±17.2)岁。21例行改良肩关节镜下双纽扣钢板动态固定术(A组),30例行锁骨钩钢板切开复位内固定术(B组)。比较两组术中及术后住院情况,采用Constant-Murley评分、加利福尼亚大学洛杉矶分校(University of California at Los Angeles,UCLA)评分、疼痛视觉模拟评分(visual analogue scale,VAS)评估肩关节功能。结果患者术后随访24~29个月,平均(24.4±5.2)个月。相较于B组,A组术中出血量更少,住院时间更短,住院总费用更低,但手术时间更长,差异均有统计学意义(P<0.05)。术后3周、6周、12周,A组的Constant-Murley评分、UCLA评分和VAS均优于B组(P<0.05);末次随访时以上3项评价指标组间比较差异均无统计学意义(P>0.05)。两组Constant-Murley评分和UCLA评分术后生存率比较,术后早期A组明显优于B组,末次随访时两组差异无统计学意义。结论对于RockwoodⅢ~Ⅴ型肩锁关节脱位,术后早期(术后3周、6周、12周)关节镜下改良纽扣钢板动态固定更有优势,术后中期(术后24个月)两种方式的治疗效果差异无统计学意义。
