Expanded bed adsorption (EBA) has been widely used in industrial downstream bioprocessing. Solid matrix is the principal pillar supporting the successful application of EBA. A novel spherical cellulose-titanium dioxid...Expanded bed adsorption (EBA) has been widely used in industrial downstream bioprocessing. Solid matrix is the principal pillar supporting the successful application of EBA. A novel spherical cellulose-titanium dioxide composite matrix was prepared through the method of water-in-oil suspension thermal regeneration. Its typical physical properties were wet density 1.18g.cm-3, diameters in the range of 100-300um, porosity 85.5%, and water content 72.3%. Expansion characteristics and liquid mixing performance of the matrix in expanded bed were investigated using water and 10% (by mass) glycerol solution as mobile phases. The results indicate that the custom-assembled matrix has a stable flow hydrodynamics and exhibits the same degree of liquid-phase mixing or column efficiency as the commercially available Streamline adsorbent.展开更多
Expanded bed adsorption(EBA),a promising and practical separation technique,has been widely studied in the past two decades.The development of adsorbents for EBA process is a challenging course,with the special design...Expanded bed adsorption(EBA),a promising and practical separation technique,has been widely studied in the past two decades.The development of adsorbents for EBA process is a challenging course,with the special design and preparation according to the target molecules and specific expanded bed systems.Many types of supporting matrices for expanded bed adsorbents have been developed,and their preparation methods are being consummated gradually.These matrices are activated and then coupled with ligands to form functionalized adsorbents,including ion-exchange adsorbents,affinity adsorbents,mixed mode adsorbents,hydrophobic charge induction chromatography adsorbents and others.In this review,the preparation of the matrices for EBA process is summa-rized,and the coupling of ligands to the matrices to prepare functionalized adsorbents is discussed as well.展开更多
Expanded bed adsorption (EBA) has been introduced as a primary recovery step for protein purification from a whole fermentation broth or unclarified cell homogenates. It can also be integrated with a fermentation or c...Expanded bed adsorption (EBA) has been introduced as a primary recovery step for protein purification from a whole fermentation broth or unclarified cell homogenates. It can also be integrated with a fermentation or cell disruption process. Solid matrix is the principal pillar supporting the successful application of the EBA technology. This article summarizes the solid matrices employed in and developed for the EBA process to date. Further development of solid matrices for the expanded bed technique in the recovery of various biological substances from different sources has been addressed.展开更多
The TiO2-densified cellulose composite beads were activated by epichlorohydrin and coupled with diethylamine, to function as an anion exchanger for expanded bed chromatography. The adsorbent exhibited a favorable perf...The TiO2-densified cellulose composite beads were activated by epichlorohydrin and coupled with diethylamine, to function as an anion exchanger for expanded bed chromatography. The adsorbent exhibited a favorable performance of expanded bed adsorption for proteins, and therefore was applied to the expanded bed recovery of D-2-chloropropionic acid dehalogenase directly from the unclarified homogenate of Pseudomonas sp. NT21. The binding capacity of the dehalogenase was found to be 8.54U·ml^-1 adsorbent, and two active peaks were eluted respectively at 0.15mol·L^-1 and 0.3mol·L^-1 （NH4）2SO4. The result indicated that the overall enzyme yield was 68%, with a purification factor of 22. In comparison to other recovery processes, the yield of the expanded bed process rises at least 70%, simultaneously saving a great deal of operation time and costs.展开更多
Expanded bed adsorption (EBA) is an integrative downstream processing technique for the purification of biological substances directly from unclarified feedstock. In this study, molecular chaperone GroEL, an important...Expanded bed adsorption (EBA) is an integrative downstream processing technique for the purification of biological substances directly from unclarified feedstock. In this study, molecular chaperone GroEL, an important protein folding helper both in vivo and in vitro, was purified by the single-step EBA technique from the unclarified homogenate of recombinant E. coli cells. Compared with packed bed adsorption, the EBA technique provided a single-step approach to yield an electrophoretic purity of GroEL. After the homogenate loading and column washing in the expanded bed mode, the GroEL protein was recovered by stepwise salt-gradient elution in packed-bed or expanded-bed modes, respectively. The expanded-bed elution mode was found as efficient as the packed-bed mode in the purification of GroEL from cell disruptate.展开更多
基金Supported by the National Natural Science Foundation of China(No.20076042,No.20206029)and the Scientific Research Foundation of the State Education Ministry for the Returned Overseas Chinese Scholars(No.2002-247).
文摘Expanded bed adsorption (EBA) has been widely used in industrial downstream bioprocessing. Solid matrix is the principal pillar supporting the successful application of EBA. A novel spherical cellulose-titanium dioxide composite matrix was prepared through the method of water-in-oil suspension thermal regeneration. Its typical physical properties were wet density 1.18g.cm-3, diameters in the range of 100-300um, porosity 85.5%, and water content 72.3%. Expansion characteristics and liquid mixing performance of the matrix in expanded bed were investigated using water and 10% (by mass) glycerol solution as mobile phases. The results indicate that the custom-assembled matrix has a stable flow hydrodynamics and exhibits the same degree of liquid-phase mixing or column efficiency as the commercially available Streamline adsorbent.
基金Supported by the National Natural Science Foundation of China （20876139, 20776129） and the National Basic Research Program of China （2007CB707805）.
文摘Expanded bed adsorption(EBA),a promising and practical separation technique,has been widely studied in the past two decades.The development of adsorbents for EBA process is a challenging course,with the special design and preparation according to the target molecules and specific expanded bed systems.Many types of supporting matrices for expanded bed adsorbents have been developed,and their preparation methods are being consummated gradually.These matrices are activated and then coupled with ligands to form functionalized adsorbents,including ion-exchange adsorbents,affinity adsorbents,mixed mode adsorbents,hydrophobic charge induction chromatography adsorbents and others.In this review,the preparation of the matrices for EBA process is summa-rized,and the coupling of ligands to the matrices to prepare functionalized adsorbents is discussed as well.
文摘Expanded bed adsorption (EBA) has been introduced as a primary recovery step for protein purification from a whole fermentation broth or unclarified cell homogenates. It can also be integrated with a fermentation or cell disruption process. Solid matrix is the principal pillar supporting the successful application of the EBA technology. This article summarizes the solid matrices employed in and developed for the EBA process to date. Further development of solid matrices for the expanded bed technique in the recovery of various biological substances from different sources has been addressed.
基金the Natural Science Foundation of Ningbo （No.2005A610018）.ACKN0WLEDGEMENTS The authors would like to thank Mr. Qi Nan for taking part in this study, and for financial support by the Natural Science Foundation of Ningbo. Discussion with Dr. Lin Dong-Qiang has been helpful and is appreciated.
文摘The TiO2-densified cellulose composite beads were activated by epichlorohydrin and coupled with diethylamine, to function as an anion exchanger for expanded bed chromatography. The adsorbent exhibited a favorable performance of expanded bed adsorption for proteins, and therefore was applied to the expanded bed recovery of D-2-chloropropionic acid dehalogenase directly from the unclarified homogenate of Pseudomonas sp. NT21. The binding capacity of the dehalogenase was found to be 8.54U·ml^-1 adsorbent, and two active peaks were eluted respectively at 0.15mol·L^-1 and 0.3mol·L^-1 （NH4）2SO4. The result indicated that the overall enzyme yield was 68%, with a purification factor of 22. In comparison to other recovery processes, the yield of the expanded bed process rises at least 70%, simultaneously saving a great deal of operation time and costs.
基金Supported by the National Natural Science Foundation of China (No. 20025617).
文摘Expanded bed adsorption (EBA) is an integrative downstream processing technique for the purification of biological substances directly from unclarified feedstock. In this study, molecular chaperone GroEL, an important protein folding helper both in vivo and in vitro, was purified by the single-step EBA technique from the unclarified homogenate of recombinant E. coli cells. Compared with packed bed adsorption, the EBA technique provided a single-step approach to yield an electrophoretic purity of GroEL. After the homogenate loading and column washing in the expanded bed mode, the GroEL protein was recovered by stepwise salt-gradient elution in packed-bed or expanded-bed modes, respectively. The expanded-bed elution mode was found as efficient as the packed-bed mode in the purification of GroEL from cell disruptate.
