This study aimed to explore the effect of Bifidobacterium animalis F1-7 on the improvement of atherosclerotic inflammation.Arteriosclerosis model ApoE^(-/-)mice were orally administered with B.animalis F1-7 for 12 wee...This study aimed to explore the effect of Bifidobacterium animalis F1-7 on the improvement of atherosclerotic inflammation.Arteriosclerosis model ApoE^(-/-)mice were orally administered with B.animalis F1-7 for 12 weeks.The probiotic intervention reduced the plaque areas in aorta and the accumulation of macrophages,and downregulated the expression of toll-like receptor 4(TLR4)/nuclear factorκB(NF-κB)pathway to reduce the levels of inflammatory factors.The widely-targeted metabolomics analysis showed that acetyl-L-carnitine(ALC)in the intestine of atherosclerotic mice was significantly increased after B.animalis F1-7 intervention.Correlation analysis proved that ALC was associated with atherosclerotic inflammatory response.By using oxidized low density lipoprotein induced macrophage foam cells,we further verified that ALC could reduce lipid accumulation and inflammatory response in foam cells by downregulating the TLR4/NF-κB pathway.Finally,our results revealed that B.animalis F1-7 upregulated the metabolite ALC to downregulate the inflammatory responses,leading to the reduction of plaque accumulation of atherosclerosis.展开更多
[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavo...[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.展开更多
In this paper the present authors measured the gas-particle two-phase velocity correlation in sudden expansion gas-particle flows with a phase Doppler particle anemometer （PDPA） and simulated the system behavior by ...In this paper the present authors measured the gas-particle two-phase velocity correlation in sudden expansion gas-particle flows with a phase Doppler particle anemometer （PDPA） and simulated the system behavior by using both a Reynolds-averaged Navier-Stokes （RANS） model and a large-eddy simulation （LES）. The results of the measurements yield the axial and radial time-averaged velocities as well as the fluctuation velocities of gas and three particle-size groups （30μm, 50μm, and 95μm） and the gasparticle velocity correlation for 30μm and 50μm particles. From the measurements, theoretical analysis, and simulation, it is found that the two-phase velocity correlation of sudden-expansion flows, like that of jet flows, is less than the gas and particle Reynolds stresses. What distinguishes the two-phase velocity correlations of sudden-expansion flow from those of jet and channel flows is the absence of a clear relationship between the two-phase velocity correlation and particle size in sudden-expansion flows. The measurements, theoretical analysis, and numerical simulation all lead to the above-stated conclusions. Quantitatively, the results of the LES are better than those of the RANS model.展开更多
We present an F-expansion method for finding periodic wave solutions of nonlinear evolution equations in mathematical physics, which can be thought of as a concentration of extended Jacobi elliptic function expansion ...We present an F-expansion method for finding periodic wave solutions of nonlinear evolution equations in mathematical physics, which can be thought of as a concentration of extended Jacobi elliptic function expansion method proposed recently. By using the F-expansion, without calculating Jacobi elliptic functions, we obtain simultaneously many periodic wave solutions expressed by various Jacobi elliptic functions for the variant Boussinesq equations. When the modulus m approaches 1 and 0, the hyperbolic function solutions (including the solitary wave solutions) and trigonometric solutions are also given respectively.展开更多
An extended F-expansion method for finding periodic wave solutions of nonlinear evolution equations in mathematical physics is presented, which can be thought of as a concentration of extended Jacobi elliptic function...An extended F-expansion method for finding periodic wave solutions of nonlinear evolution equations in mathematical physics is presented, which can be thought of as a concentration of extended Jacobi elliptic function expansion method proposed more recently. By using the homogeneous balance principle and the extended F-expansion, more periodic wave solutions expressed by Jacobi elliptic functions for the coupled KdV equations are derived. In the limit cases, the solitary wave solutions and the other type of travelling wave solutions for the system are also obtained.展开更多
我们在场为在数学物理发现非线性的进化方程的周期的波浪答案的一个扩大 F 扩大方法。由使用扩大 F 扩大方法，各种各样的 Jacobi 椭圆形的功能为 theKlein-Gordon-Schroedinger 方程表示的许多周期的波浪答案被获得。在限制盒子中，方...我们在场为在数学物理发现非线性的进化方程的周期的波浪答案的一个扩大 F 扩大方法。由使用扩大 F 扩大方法，各种各样的 Jacobi 椭圆形的功能为 theKlein-Gordon-Schroedinger 方程表示的许多周期的波浪答案被获得。在限制盒子中，方程的独居的波浪解决方案和三角法的函数解决方案也被获得。展开更多
A generalized F-expansion method is introduced and applied to (3+ 1)-dimensional Kadomstev-Petviashvili(KP) equation. As a result, some new Jacobi elliptic function solutions of the equation are found, from which the ...A generalized F-expansion method is introduced and applied to (3+ 1)-dimensional Kadomstev-Petviashvili(KP) equation. As a result, some new Jacobi elliptic function solutions of the equation are found, from which the trigonometric function solutions and the solitary wave solutions can be obtained. The method can also be extended to other types of nonlinear evolution equations in mathematical physics.展开更多
基金supported by Shandong Taishan industry leading talent project(LJNY202101)the National Key R&D of China(2018YFC0311201)。
文摘This study aimed to explore the effect of Bifidobacterium animalis F1-7 on the improvement of atherosclerotic inflammation.Arteriosclerosis model ApoE^(-/-)mice were orally administered with B.animalis F1-7 for 12 weeks.The probiotic intervention reduced the plaque areas in aorta and the accumulation of macrophages,and downregulated the expression of toll-like receptor 4(TLR4)/nuclear factorκB(NF-κB)pathway to reduce the levels of inflammatory factors.The widely-targeted metabolomics analysis showed that acetyl-L-carnitine(ALC)in the intestine of atherosclerotic mice was significantly increased after B.animalis F1-7 intervention.Correlation analysis proved that ALC was associated with atherosclerotic inflammatory response.By using oxidized low density lipoprotein induced macrophage foam cells,we further verified that ALC could reduce lipid accumulation and inflammatory response in foam cells by downregulating the TLR4/NF-κB pathway.Finally,our results revealed that B.animalis F1-7 upregulated the metabolite ALC to downregulate the inflammatory responses,leading to the reduction of plaque accumulation of atherosclerosis.
基金Supported by Youth Fund Project of Zhaoqing University(QZ202235)Zhaoqing Science and Technology Plan Project(2022040311011).
文摘[Objectives]To study the inhibitory activity of two flavonoid glycosides isolated from Chlorophytum comosum Laxum R.Br on human nasopharyngeal carcinoma(NPC)cell line 5-8F in vitro and its mechanism.[Methods]The flavonoid glycosides were isolated and purified from the ethanol alcoholic extract of the roots of Liliaceae plant Chlorophytum comosum by silica gel column chromatography,macroporous resin column chromatography,Sephadex LH-20,and reverse column chromatography(ODS).The inhibitory activity of flavonoid glycosides on human nasopharyngeal carcinoma cells was analyzed by CCK-8 method,and the potential mechanism was preliminarily analyzed by molecular docking.[Results]Two flavonoid glycosides were identified as isovitexin 2″-0-rhamnoside and 7-2″-di-O-β-glucopyranosylisovitexin.Two flavonoid glycosides showed promising inhibitory effect on human nasopharyngeal carcinoma cell line 5-8F,with IC_(50) values of 24.8 and 27.5μmol/L,respectively.Molecular docking results showed that the potential targets of two flavonoid glycosides include CyclinD1,Bcl-2β-Catenin,ILK,TGF-β,in addition,two glycosides showed higher predicted binding affinity towards CyclinD1,which verifies the cytotoxicity of the two compounds on human nasopharyngeal carcinoma cell line 5-8F in vitro.[Conclusions]Two flavonoid glycosides are the active molecules in Chlorophytum comosum that can inhibit the proliferation of human nasopharyngeal carcinoma cells,and have the potential to be used in the research and development of anti nasopharyngeal carcinoma drugs.
基金supported by the National Natural Science Foundation of China (50606026 and 50736006)
文摘In this paper the present authors measured the gas-particle two-phase velocity correlation in sudden expansion gas-particle flows with a phase Doppler particle anemometer （PDPA） and simulated the system behavior by using both a Reynolds-averaged Navier-Stokes （RANS） model and a large-eddy simulation （LES）. The results of the measurements yield the axial and radial time-averaged velocities as well as the fluctuation velocities of gas and three particle-size groups （30μm, 50μm, and 95μm） and the gasparticle velocity correlation for 30μm and 50μm particles. From the measurements, theoretical analysis, and simulation, it is found that the two-phase velocity correlation of sudden-expansion flows, like that of jet flows, is less than the gas and particle Reynolds stresses. What distinguishes the two-phase velocity correlations of sudden-expansion flow from those of jet and channel flows is the absence of a clear relationship between the two-phase velocity correlation and particle size in sudden-expansion flows. The measurements, theoretical analysis, and numerical simulation all lead to the above-stated conclusions. Quantitatively, the results of the LES are better than those of the RANS model.
基金河南省自然科学基金，河南省教育厅自然科学基金，the Science Foundation of Henan University of Science and Technology
文摘We present an F-expansion method for finding periodic wave solutions of nonlinear evolution equations in mathematical physics, which can be thought of as a concentration of extended Jacobi elliptic function expansion method proposed recently. By using the F-expansion, without calculating Jacobi elliptic functions, we obtain simultaneously many periodic wave solutions expressed by various Jacobi elliptic functions for the variant Boussinesq equations. When the modulus m approaches 1 and 0, the hyperbolic function solutions (including the solitary wave solutions) and trigonometric solutions are also given respectively.
基金Project supported by the Natural Science Foundation of Henan Province of China （Grant No 0111050200） and the Science Foundation of Henan University of Science and Technology （Grant Nos 2004ZY040 and 2004ZD002）.
文摘An extended F-expansion method for finding periodic wave solutions of nonlinear evolution equations in mathematical physics is presented, which can be thought of as a concentration of extended Jacobi elliptic function expansion method proposed more recently. By using the homogeneous balance principle and the extended F-expansion, more periodic wave solutions expressed by Jacobi elliptic functions for the coupled KdV equations are derived. In the limit cases, the solitary wave solutions and the other type of travelling wave solutions for the system are also obtained.
基金The project supported by the Major Project of National Natural Science Foundation of China under Grant No. 49894190 and the Knowledge Innovation Project of CAS under Grant No. KZCXl-sw-18
基金The project supported by the Natural Science Foundation of Eduction Committce of Henan Province of China under Grant No. 2003110003, and the Science Foundation of Henan University of Science and Technology under Grant Nos. 2004ZD002 and 2004ZY040
文摘我们在场为在数学物理发现非线性的进化方程的周期的波浪答案的一个扩大 F 扩大方法。由使用扩大 F 扩大方法，各种各样的 Jacobi 椭圆形的功能为 theKlein-Gordon-Schroedinger 方程表示的许多周期的波浪答案被获得。在限制盒子中，方程的独居的波浪解决方案和三角法的函数解决方案也被获得。
文摘A generalized F-expansion method is introduced and applied to (3+ 1)-dimensional Kadomstev-Petviashvili(KP) equation. As a result, some new Jacobi elliptic function solutions of the equation are found, from which the trigonometric function solutions and the solitary wave solutions can be obtained. The method can also be extended to other types of nonlinear evolution equations in mathematical physics.
