The chromosome constitution of hybrids and chromation patterns of Agropyro-nelelongatum(Host)Neviski in F5 somatic hybrid lines Ⅱ -1-3 and l-1-9 between Triticum aesti-vum L.and A.elongatum were analyzed.Based on the...The chromosome constitution of hybrids and chromation patterns of Agropyro-nelelongatum(Host)Neviski in F5 somatic hybrid lines Ⅱ -1-3 and l-1-9 between Triticum aesti-vum L.and A.elongatum were analyzed.Based on the statistic data of pollen omther cells,F5 l-1-9 and Ⅱ-1-3 had 20—21 bivalents with a frequency of 84.66% and 85.28%,of which,89.83% and 89.57% were ring bivalents.The result indicated that both hybrid lines were basically stable in the chromosome constitution and behavior.RAPD analysis showed that the two hybrids contained biparental and integrated DNA.GISH(Genome in situ hybridization)revealed that in the form of small chromosome segments,A.elongatum chromatin was scattered on 4—6 wheat chromo-somes near by the region of centromere and telomere in the two hybrid lines.SSR analysis in-dicated that A.elongatum DAN segments were distributed on the 2A,5B,6B and 2D wheat chromosomes in the hybrids,which was in accordance with the GISH results that small-segments intercalated poly-site.展开更多
Coexistence of chronic lymphocytic leukemia （CLL） and essential thrombocythemia （ET） in a patient is extremely rare, with only 10 cases reported thus far in literature. This paper describes a 94-year-old male havi...Coexistence of chronic lymphocytic leukemia （CLL） and essential thrombocythemia （ET） in a patient is extremely rare, with only 10 cases reported thus far in literature. This paper describes a 94-year-old male having atypical B-CLL with CD5- （CD5-） phenotype and ET. In this patient, we performed interphase fluorescence in situ hybridization （FISH） analysis which revealed 13q14.3 deletion in 31% of B-lymphocyte nuclei and RB1 deletion in 27% of B-lymphocyte nuclei, but not in neutrophils and T-lymphocytes. Furthermore, we identified JAK2 V617F mutation in the peripheral blood nucleated cells and neutrophils, but not in the B- and T-lymphocyte populations. Therefore, it was concluded that the occurrence of CD5- B-CLL and ET in this patient was pathogenically independent.展开更多
基金This work was supported by the National Natural Science Foundation of China(Grant No.30370857)Major Project of Ministry of Education in ChinaNational 863 High Technology Research and Development Project(No.2001AA241032).
文摘The chromosome constitution of hybrids and chromation patterns of Agropyro-nelelongatum(Host)Neviski in F5 somatic hybrid lines Ⅱ -1-3 and l-1-9 between Triticum aesti-vum L.and A.elongatum were analyzed.Based on the statistic data of pollen omther cells,F5 l-1-9 and Ⅱ-1-3 had 20—21 bivalents with a frequency of 84.66% and 85.28%,of which,89.83% and 89.57% were ring bivalents.The result indicated that both hybrid lines were basically stable in the chromosome constitution and behavior.RAPD analysis showed that the two hybrids contained biparental and integrated DNA.GISH(Genome in situ hybridization)revealed that in the form of small chromosome segments,A.elongatum chromatin was scattered on 4—6 wheat chromo-somes near by the region of centromere and telomere in the two hybrid lines.SSR analysis in-dicated that A.elongatum DAN segments were distributed on the 2A,5B,6B and 2D wheat chromosomes in the hybrids,which was in accordance with the GISH results that small-segments intercalated poly-site.
文摘Coexistence of chronic lymphocytic leukemia （CLL） and essential thrombocythemia （ET） in a patient is extremely rare, with only 10 cases reported thus far in literature. This paper describes a 94-year-old male having atypical B-CLL with CD5- （CD5-） phenotype and ET. In this patient, we performed interphase fluorescence in situ hybridization （FISH） analysis which revealed 13q14.3 deletion in 31% of B-lymphocyte nuclei and RB1 deletion in 27% of B-lymphocyte nuclei, but not in neutrophils and T-lymphocytes. Furthermore, we identified JAK2 V617F mutation in the peripheral blood nucleated cells and neutrophils, but not in the B- and T-lymphocyte populations. Therefore, it was concluded that the occurrence of CD5- B-CLL and ET in this patient was pathogenically independent.
