Detection of F. solani f. sp. cucurbitae causal agent of the crown and root rot disease of melon race 1, race 2 is difficult. It is based only on morphological characteristic. In this study, forty isolates identified ...Detection of F. solani f. sp. cucurbitae causal agent of the crown and root rot disease of melon race 1, race 2 is difficult. It is based only on morphological characteristic. In this study, forty isolates identified as Fusarium solani based on morphological characterization, F. solani was one of the most frequently isolated species. Molecular identification of these isolates by PCR technique using species-specific primer, indicated that thirty-two isolates, amplified product 580 bp (race 1) and two isolate amplified product 580 bp (race 2), while six isolates were not amplified with primer of both races. Production of Trichothecenes (T2-toxen, DON.) by Fusarium solani was conducted on isolates confirmed as belonging in the F. solani by PCR. The results indicated that the presence of Tri5, Tri13 genes is coding the ability of synthesis mycotoxin. In vitro, the results indicated that NPs (AgNPs, MgNPs) and chemical (Phylex) possess the antifungal properties against at various level. Treatment with (AgNPs 150 ppm, MgNPs 2%, 3% ppm) and 3% Phylex resulted in maximum inhabitation of F. solani . In vivo, five characters (height plant, hoot ant root fresh and dry weight) were measured based on the greenhouse, field experimental results. Treatment with (AgNPs, MgNPs) and Phylex had higher measured parameters than positive control.展开更多
1991年在浙江湖州市和杭州市采集得百合(Lilium brownii F. E. Brown var. viridulum Baker)鳞茎有褐色斑点的标本104份,分离出纯菌株108个。鉴定为2个镰刀菌种:Fusarium solani(Mart.)Sacc. 和F. oxysporum Schlecht. F. solani出现频...1991年在浙江湖州市和杭州市采集得百合(Lilium brownii F. E. Brown var. viridulum Baker)鳞茎有褐色斑点的标本104份,分离出纯菌株108个。鉴定为2个镰刀菌种:Fusarium solani(Mart.)Sacc. 和F. oxysporum Schlecht. F. solani出现频率84.3％,占优势.田间调查和接种试验证明F.solani是百合鳞茎褐色斑点病主要致病菌。在20种植物上接种试验结果表明:F.solani对百合有专化性,因此鉴定其病原菌为茄病镰孢一个新专化型:Fusarium solajni f. sp.lilii展开更多
文摘Detection of F. solani f. sp. cucurbitae causal agent of the crown and root rot disease of melon race 1, race 2 is difficult. It is based only on morphological characteristic. In this study, forty isolates identified as Fusarium solani based on morphological characterization, F. solani was one of the most frequently isolated species. Molecular identification of these isolates by PCR technique using species-specific primer, indicated that thirty-two isolates, amplified product 580 bp (race 1) and two isolate amplified product 580 bp (race 2), while six isolates were not amplified with primer of both races. Production of Trichothecenes (T2-toxen, DON.) by Fusarium solani was conducted on isolates confirmed as belonging in the F. solani by PCR. The results indicated that the presence of Tri5, Tri13 genes is coding the ability of synthesis mycotoxin. In vitro, the results indicated that NPs (AgNPs, MgNPs) and chemical (Phylex) possess the antifungal properties against at various level. Treatment with (AgNPs 150 ppm, MgNPs 2%, 3% ppm) and 3% Phylex resulted in maximum inhabitation of F. solani . In vivo, five characters (height plant, hoot ant root fresh and dry weight) were measured based on the greenhouse, field experimental results. Treatment with (AgNPs, MgNPs) and Phylex had higher measured parameters than positive control.
文摘1991年在浙江湖州市和杭州市采集得百合(Lilium brownii F. E. Brown var. viridulum Baker)鳞茎有褐色斑点的标本104份,分离出纯菌株108个。鉴定为2个镰刀菌种:Fusarium solani(Mart.)Sacc. 和F. oxysporum Schlecht. F. solani出现频率84.3％,占优势.田间调查和接种试验证明F.solani是百合鳞茎褐色斑点病主要致病菌。在20种植物上接种试验结果表明:F.solani对百合有专化性,因此鉴定其病原菌为茄病镰孢一个新专化型:Fusarium solajni f. sp.lilii
文摘【目的】鉴定龙爪槐枝干溃疡病的病原菌。【方法】采用组织分离法从龙爪槐枝干溃疡病病枝获得疑似病原菌,并通过野外致病性测定该病原菌,结合该病原菌的形态学特征、r DNA-ITS和EF-α基因序列分析,进行病原菌的种类鉴定。【结果】完成了F6菌株致病性分析的科赫法则实验,证实了其致病性;鉴定该菌为腐皮镰孢菌复合种[Fusarium solani species complex(FSSC)]。【结论】腐皮镰孢菌复合种(FSSC)为引起龙爪槐枝干溃疡病的病原菌。
