目的:探讨胃腺癌中Fas相关死亡结构域蛋白(FADD)与半胱氨酸蛋白酶-7蛋白(Caspase-7)的表达及意义。方法:采用免疫组化S P法检测60例胃腺癌组织及50例正常胃黏膜组织F A D D蛋白与C a s p a s e-7蛋白的表达情况,分析二者与胃腺癌临床病...目的:探讨胃腺癌中Fas相关死亡结构域蛋白(FADD)与半胱氨酸蛋白酶-7蛋白(Caspase-7)的表达及意义。方法:采用免疫组化S P法检测60例胃腺癌组织及50例正常胃黏膜组织F A D D蛋白与C a s p a s e-7蛋白的表达情况,分析二者与胃腺癌临床病理特征的关系,以及胃腺癌组织中二者表达的相关性。结果:FADD蛋白和Caspase-7蛋白阳性产物均为棕黄色颗粒状,主要位于细胞质。胃腺癌组织FADD蛋白和Caspase-7蛋白的阳性表达率均明显低于正常胃黏膜组织(38.3%vs 60.0%,46.7%vs 68.0%,P<0.05)。FADD蛋白、Caspase-7蛋白表达与胃腺癌患者性别、年龄无关(P>0.05),与胃腺癌分化程度、T N M分期、淋巴结转移、浸润深度有关。Spearman相关分析结果显示,胃腺癌FADD蛋白和Caspase-7蛋白表达呈正相关关系(r=0.362,P<0.05)。结论:FADD蛋白与Caspase-7蛋白低表达可能在胃腺癌的发生发展过程中具有协同作用。展开更多
Although cholecystokinin octapeptide-8 is important for neurological function, its neuroprotective properties remain unclear. We speculated that cholecystokinin octapeptide-8 can protect human retinal pigment epitheli...Although cholecystokinin octapeptide-8 is important for neurological function, its neuroprotective properties remain unclear. We speculated that cholecystokinin octapeptide-8 can protect human retinal pigment epithelial cells against oxidative injury. In this study, retinal pigment epithelial cells were treated with peroxynitrite to induce oxidative stress. Peroxynitrite triggered apoptosis in these cells, and increased the expression of Fas-associated death domain, Bax, caspase-8 and Bcl-2. These changes were suppressed by treatment with cholecystokinin octapeptide-8. These results suggest that cholecystokinin octapeptide-8 can protect human retinal pigment epithelial cells against apoptosis induced by peroxynitrite.展开更多
Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic...Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biological correlates of the pro-apoptotic marker Fas and the anti-apoptotic marker Bcl-xL in sperm cells of fertile men. Six hundred and four men from Greenland, Poland and Ukraine were consecutively enrolled during their pregnant wife＇s antenatal visits. Semen analysis was performed as recommended by the World Health Organization. Immunofluorescence coupled to flow cytometry was utilized for detection of apoptotic markers in the sperm cell. DNA damage was assessed by flow cytometry using both the sperm chromatin structure assay （SCSA） and the terminal deoxynucleotidyl transferase dUTP nick end labelling （TUNEL） assay. The percentage of Fas-positive sperm cells was higher in men with high total sperm count （P〈O.01）, more motile sperms （P=-O.04） and fewer sperm head defects （P=-O.05）. These associations were consistent within and across study regions. Furthermore, testosterone, follicle-stimulating hormone （FSH） and sexual hormone-binding globulin （SHBG） were significantly negatively correlated with Fas within and across regions as well. The data indicated no association between the anti-apoptotic Bcl-xL marker and semen or personal characteristics. The finding of Fas-positive sperm cells associated with better semen quality in a cohort of spouses of pregnant women seems different from previous data obtained in infertile men and warrants further investigation to clarify the biological significance of sperm apoptotic markers.展开更多
文摘目的:探讨胃腺癌中Fas相关死亡结构域蛋白(FADD)与半胱氨酸蛋白酶-7蛋白(Caspase-7)的表达及意义。方法:采用免疫组化S P法检测60例胃腺癌组织及50例正常胃黏膜组织F A D D蛋白与C a s p a s e-7蛋白的表达情况,分析二者与胃腺癌临床病理特征的关系,以及胃腺癌组织中二者表达的相关性。结果:FADD蛋白和Caspase-7蛋白阳性产物均为棕黄色颗粒状,主要位于细胞质。胃腺癌组织FADD蛋白和Caspase-7蛋白的阳性表达率均明显低于正常胃黏膜组织(38.3%vs 60.0%,46.7%vs 68.0%,P<0.05)。FADD蛋白、Caspase-7蛋白表达与胃腺癌患者性别、年龄无关(P>0.05),与胃腺癌分化程度、T N M分期、淋巴结转移、浸润深度有关。Spearman相关分析结果显示,胃腺癌FADD蛋白和Caspase-7蛋白表达呈正相关关系(r=0.362,P<0.05)。结论:FADD蛋白与Caspase-7蛋白低表达可能在胃腺癌的发生发展过程中具有协同作用。
文摘Although cholecystokinin octapeptide-8 is important for neurological function, its neuroprotective properties remain unclear. We speculated that cholecystokinin octapeptide-8 can protect human retinal pigment epithelial cells against oxidative injury. In this study, retinal pigment epithelial cells were treated with peroxynitrite to induce oxidative stress. Peroxynitrite triggered apoptosis in these cells, and increased the expression of Fas-associated death domain, Bax, caspase-8 and Bcl-2. These changes were suppressed by treatment with cholecystokinin octapeptide-8. These results suggest that cholecystokinin octapeptide-8 can protect human retinal pigment epithelial cells against apoptosis induced by peroxynitrite.
文摘Apoptosis in the testis has two putative roles during normal spermatogenesis; limitation of the germ cell population to numbers that can be supported by the Sertoli cells, and, possibly, selective depletion of meiotic and postmeiotic abnormal germ cells. We investigated the demographic and biological correlates of the pro-apoptotic marker Fas and the anti-apoptotic marker Bcl-xL in sperm cells of fertile men. Six hundred and four men from Greenland, Poland and Ukraine were consecutively enrolled during their pregnant wife＇s antenatal visits. Semen analysis was performed as recommended by the World Health Organization. Immunofluorescence coupled to flow cytometry was utilized for detection of apoptotic markers in the sperm cell. DNA damage was assessed by flow cytometry using both the sperm chromatin structure assay （SCSA） and the terminal deoxynucleotidyl transferase dUTP nick end labelling （TUNEL） assay. The percentage of Fas-positive sperm cells was higher in men with high total sperm count （P〈O.01）, more motile sperms （P=-O.04） and fewer sperm head defects （P=-O.05）. These associations were consistent within and across study regions. Furthermore, testosterone, follicle-stimulating hormone （FSH） and sexual hormone-binding globulin （SHBG） were significantly negatively correlated with Fas within and across regions as well. The data indicated no association between the anti-apoptotic Bcl-xL marker and semen or personal characteristics. The finding of Fas-positive sperm cells associated with better semen quality in a cohort of spouses of pregnant women seems different from previous data obtained in infertile men and warrants further investigation to clarify the biological significance of sperm apoptotic markers.
