Calcium plays a crucial role in the normal and abnormal cell metabolism. The role of calcium in the differentiation process of murine erythroleukemia cells(MELC) remains controversial. Here, based upon quantitative me...Calcium plays a crucial role in the normal and abnormal cell metabolism. The role of calcium in the differentiation process of murine erythroleukemia cells(MELC) remains controversial. Here, based upon quantitative measurement of fluorescence in single cells, a method was developed to investigate the intracellular free calcium [Ca2+]i concentration and DNA contents simultaneously, by employing the fluorescent probe, fluo-3 acetoxymethyl ester and DNA dye Hoechst 33342. During MELC differentiation, [Ca2+]i concentration incresed. We also demonstrated that calcium ionophore, A23187, enhanced the HMBA-induced MELC differentiation, while verapamil, an inhibitor of calcuim uptake, slightly reduced differentiation. These results suggested that an increase in the [Ca2+]i level was an essential step in HMBA-induced MELC differentiation.展开更多
目的:研究双吲哚马来酰亚胺衍生物GZWM-051(简称化合物GZWM-051)对人白血病HEL细胞周期及分化的影响。方法: HEL细胞分为对照组(DMSO处理)和化合物GZWM-051组(0.025、0.050、0.100 μmol/L化合物GZWM-051处理,分别为低剂量、中剂量、高...目的:研究双吲哚马来酰亚胺衍生物GZWM-051(简称化合物GZWM-051)对人白血病HEL细胞周期及分化的影响。方法: HEL细胞分为对照组(DMSO处理)和化合物GZWM-051组(0.025、0.050、0.100 μmol/L化合物GZWM-051处理,分别为低剂量、中剂量、高剂量组),采用流式细胞术检测细胞周期及分化水平,采用Western blot法检测细胞Cyclin B1、c-Myc、STAT3及P-STAT3蛋白表达水平。结果:处理24 h后,与对照组HEL细胞相比,化合物GZWM-051中、高剂量组 HEL细胞处于G1和S期细胞数量显著减少,处于G2期的数量显著增加,差异有高度统计学意义( P <0.01);相比对照组,作用48 h后化合物GZWM-051低、中、高剂量组HEL细胞的CD41a和CD71均上调;与对照组HEL细胞对比,中剂量及高剂量化合物GZWM-051组HEL细胞生长相关蛋白c-Myc表达水平降低,差异有统计学意义( P <0.05或 P <0.01);与对照组HEL细胞对比,低、中及高剂量的化合物GZWM-051组HEL细胞的P-STAT3表达水平降低( P <0.05或 P <0.01),中、高剂量化合物GZWM-051组HEL细胞周期蛋白Cyclin B1表达水平降低( P <0.05)。结论:化合物GZWM-051不仅能诱导白血病HEL细胞发生G2周期阻滞,促进其向巨核及红系进行分化,抑制细胞恶性增殖,还能失活STAT3关键通路。展开更多
文摘Calcium plays a crucial role in the normal and abnormal cell metabolism. The role of calcium in the differentiation process of murine erythroleukemia cells(MELC) remains controversial. Here, based upon quantitative measurement of fluorescence in single cells, a method was developed to investigate the intracellular free calcium [Ca2+]i concentration and DNA contents simultaneously, by employing the fluorescent probe, fluo-3 acetoxymethyl ester and DNA dye Hoechst 33342. During MELC differentiation, [Ca2+]i concentration incresed. We also demonstrated that calcium ionophore, A23187, enhanced the HMBA-induced MELC differentiation, while verapamil, an inhibitor of calcuim uptake, slightly reduced differentiation. These results suggested that an increase in the [Ca2+]i level was an essential step in HMBA-induced MELC differentiation.
文摘目的:研究双吲哚马来酰亚胺衍生物GZWM-051(简称化合物GZWM-051)对人白血病HEL细胞周期及分化的影响。方法: HEL细胞分为对照组(DMSO处理)和化合物GZWM-051组(0.025、0.050、0.100 μmol/L化合物GZWM-051处理,分别为低剂量、中剂量、高剂量组),采用流式细胞术检测细胞周期及分化水平,采用Western blot法检测细胞Cyclin B1、c-Myc、STAT3及P-STAT3蛋白表达水平。结果:处理24 h后,与对照组HEL细胞相比,化合物GZWM-051中、高剂量组 HEL细胞处于G1和S期细胞数量显著减少,处于G2期的数量显著增加,差异有高度统计学意义( P <0.01);相比对照组,作用48 h后化合物GZWM-051低、中、高剂量组HEL细胞的CD41a和CD71均上调;与对照组HEL细胞对比,中剂量及高剂量化合物GZWM-051组HEL细胞生长相关蛋白c-Myc表达水平降低,差异有统计学意义( P <0.05或 P <0.01);与对照组HEL细胞对比,低、中及高剂量的化合物GZWM-051组HEL细胞的P-STAT3表达水平降低( P <0.05或 P <0.01),中、高剂量化合物GZWM-051组HEL细胞周期蛋白Cyclin B1表达水平降低( P <0.05)。结论:化合物GZWM-051不仅能诱导白血病HEL细胞发生G2周期阻滞,促进其向巨核及红系进行分化,抑制细胞恶性增殖,还能失活STAT3关键通路。
