目的探讨2-(1-{6-[(2-^(18)F-乙基)(甲基)氨]-2-萘}-乙叉)-丙二腈(^(18)F-FDDNP)PET显像条件及用于阿尔茨海默病(AD)诊断的价值。方法 7例 AD(AD 组),6例血管性痴呆(VD 组)患者及6名智力正常老年对照者(HC 组)。静脉注射^(18)F-FDDNP 后...目的探讨2-(1-{6-[(2-^(18)F-乙基)(甲基)氨]-2-萘}-乙叉)-丙二腈(^(18)F-FDDNP)PET显像条件及用于阿尔茨海默病(AD)诊断的价值。方法 7例 AD(AD 组),6例血管性痴呆(VD 组)患者及6名智力正常老年对照者(HC 组)。静脉注射^(18)F-FDDNP 后 HC 组中的3例采用连续动态采集程序扫描并生成时间-放射性曲线,余受试者在药物注射后5,25和45 min 分别采集图像。采用ROI 法进行图像分析,计算3组受试者各脑区在5～75 min 及5～45 min 的放射性清除率并进行统计分析。结果 ^(18)F-FDDNP 能快速通过血脑屏障且之后能很快从健康脑组织中洗脱。AD 患者脑内放射性清除较 HC 组慢,而 VD 患者 PET 显像与 HC 组差别不大。AD 组大脑皮质及皮质下核团放射性清除率与 HC 组比较,差异有统计学意义(P<0.05),而白质和小脑差异无统计学意义。VD 患者与HC 组比较,除纹状体外各脑区放射性清除率差别无统计学意义。结论 ^(18)F-FDDNP 符合神经系统显像剂的要求。^(18)F-FDDNP PET 脑显像能有效诊断 AD,且可以鉴别 AD 和 VD。展开更多
[18F]-FDDNP was synthesized and characterized as a positron-emitting probe to identify Alzheimer’s disease (AD) in transgenic mouse models (Tg2576 and dE9) expressing the AD pathology. We observed in in vitro, in viv...[18F]-FDDNP was synthesized and characterized as a positron-emitting probe to identify Alzheimer’s disease (AD) in transgenic mouse models (Tg2576 and dE9) expressing the AD pathology. We observed in in vitro, in vivo, and ex vivo studies that [18F]-FDDNP accumulated specifically in the Ab-overexpressing brain regions and that this accumulation was significantly reduced by co-incubation with non-radioactive FDDNP. In ex vivo and in vivo studies of brain sections, the retention of radioactivity was more specific in Tg2576 mice than in dE9 mice. Using in vitro, ex vivo, in vivo, and ELISA analyses, we characterized the utility of [18F]-FDDNP in mapping b-amyloid in the Tg2576 mouse brain, to assess its potential application in imaging strategies.展开更多
文摘目的探讨2-(1-{6-[(2-^(18)F-乙基)(甲基)氨]-2-萘}-乙叉)-丙二腈(^(18)F-FDDNP)PET显像条件及用于阿尔茨海默病(AD)诊断的价值。方法 7例 AD(AD 组),6例血管性痴呆(VD 组)患者及6名智力正常老年对照者(HC 组)。静脉注射^(18)F-FDDNP 后 HC 组中的3例采用连续动态采集程序扫描并生成时间-放射性曲线,余受试者在药物注射后5,25和45 min 分别采集图像。采用ROI 法进行图像分析,计算3组受试者各脑区在5～75 min 及5～45 min 的放射性清除率并进行统计分析。结果 ^(18)F-FDDNP 能快速通过血脑屏障且之后能很快从健康脑组织中洗脱。AD 患者脑内放射性清除较 HC 组慢,而 VD 患者 PET 显像与 HC 组差别不大。AD 组大脑皮质及皮质下核团放射性清除率与 HC 组比较,差异有统计学意义(P<0.05),而白质和小脑差异无统计学意义。VD 患者与HC 组比较,除纹状体外各脑区放射性清除率差别无统计学意义。结论 ^(18)F-FDDNP 符合神经系统显像剂的要求。^(18)F-FDDNP PET 脑显像能有效诊断 AD,且可以鉴别 AD 和 VD。
文摘[18F]-FDDNP was synthesized and characterized as a positron-emitting probe to identify Alzheimer’s disease (AD) in transgenic mouse models (Tg2576 and dE9) expressing the AD pathology. We observed in in vitro, in vivo, and ex vivo studies that [18F]-FDDNP accumulated specifically in the Ab-overexpressing brain regions and that this accumulation was significantly reduced by co-incubation with non-radioactive FDDNP. In ex vivo and in vivo studies of brain sections, the retention of radioactivity was more specific in Tg2576 mice than in dE9 mice. Using in vitro, ex vivo, in vivo, and ELISA analyses, we characterized the utility of [18F]-FDDNP in mapping b-amyloid in the Tg2576 mouse brain, to assess its potential application in imaging strategies.
