The biosorptive interaction of Ag++ with resting cell of %Lactobacillus sp.% strain A09 has been further studied on a molecular level by means of XPS, EDX, UV-Vis and FTIR techniques. The X-ray photoelectron spectrosc...The biosorptive interaction of Ag++ with resting cell of %Lactobacillus sp.% strain A09 has been further studied on a molecular level by means of XPS, EDX, UV-Vis and FTIR techniques. The X-ray photoelectron spectroscopy(XPS) shows that the reductive ratio of the Ag++ to Ag+0 by the A09 biomass reaches to about 54^5% for 3 d. The contain of amino acid residues in dry powder of the biomass such as cysteine, methionine, arginine and lysine, being capable of reducing the Ag++ to Ag+0, are very small in quantity both cysteine and methionine are far less than 0^18%, and both arginine and lysine far less than 0^336%, %via% the analysis with quantitative energy-dispersive X-ray(EDX). The amount of the reducing sugars in the biomass is far larger than 2^71% analyzed by ultraviolet-visible spectrophotometry(UV-Vis). The chemical functional group on cell wass of the biomass such as the carboxylate anion of amino-acid residues seems to be the site for the Ag++ binding and the free aldehyde group of the hemiacetalic hydroxyl from reducing sugars, %i.e.% the hydrolysates of the polysaccharides from the cell wass, plays a protagonist in serving as the electron donor for reducing the Ag++ to Ag+0, characterized by fourier transform infrared(FTIR) spectrophotometry.展开更多
The increasing-compatibility-matrix-stuff with properties of compellent compatibility was prepared through the seed emulsion grafting co-polymerization methods. The structures of the increasing-compatible-matrix-stuff...The increasing-compatibility-matrix-stuff with properties of compellent compatibility was prepared through the seed emulsion grafting co-polymerization methods. The structures of the increasing-compatible-matrix-stuff was analyzed by fourier transform infrared spectroscopy(FTIR) and nuclear magnetic resonate(NMR). The compellent damping materials was prepared using different ratio of the increasing-compatible-matrix-stuff, and the properties was tested with dynamic mechanic analysis(DMA) methods. The results show that the increasing-compatible-matrix-stuff has the special characteristics of compellent compatibility, and enhance the glass transformation temperature of the damping materials, and markedly develop the using-temperature-extent and the loss tangent of the damping materials.展开更多
In situ ATR-FTIR was used to study the interaction of bovine serum albumin（BSA） and hydroxyapatite（HA） in this work.Both on the electrochemically deposited HA and self assemblied titanate nanotubes（TNT） substrat...In situ ATR-FTIR was used to study the interaction of bovine serum albumin（BSA） and hydroxyapatite（HA） in this work.Both on the electrochemically deposited HA and self assemblied titanate nanotubes（TNT） substrates,the adsorption of BSA increases with the increase of time,and its adsorption on HA surface is much larger than that on TNT surface,implying that HA has a good biocompatibility as a biomaterial.It is indicated that Ca2+ plays an essential role in the protein absorption on the HA surface,due to that BSA is negatively charged,and it is able to combine with cation locations in HA particles by electrostatic forces.It is found that,when reacting with BSA,the phosphate band of HA in solution shifts to higher wavenumbers,indicating that PO3-4 can also be an adsorption site for BSA.The spectroscopic study on the interaction between HA and BSA will be helpful for further understanding the nano hydroxyapatite bioproperties on molecular level.展开更多
文摘The biosorptive interaction of Ag++ with resting cell of %Lactobacillus sp.% strain A09 has been further studied on a molecular level by means of XPS, EDX, UV-Vis and FTIR techniques. The X-ray photoelectron spectroscopy(XPS) shows that the reductive ratio of the Ag++ to Ag+0 by the A09 biomass reaches to about 54^5% for 3 d. The contain of amino acid residues in dry powder of the biomass such as cysteine, methionine, arginine and lysine, being capable of reducing the Ag++ to Ag+0, are very small in quantity both cysteine and methionine are far less than 0^18%, and both arginine and lysine far less than 0^336%, %via% the analysis with quantitative energy-dispersive X-ray(EDX). The amount of the reducing sugars in the biomass is far larger than 2^71% analyzed by ultraviolet-visible spectrophotometry(UV-Vis). The chemical functional group on cell wass of the biomass such as the carboxylate anion of amino-acid residues seems to be the site for the Ag++ binding and the free aldehyde group of the hemiacetalic hydroxyl from reducing sugars, %i.e.% the hydrolysates of the polysaccharides from the cell wass, plays a protagonist in serving as the electron donor for reducing the Ag++ to Ag+0, characterized by fourier transform infrared(FTIR) spectrophotometry.
文摘The increasing-compatibility-matrix-stuff with properties of compellent compatibility was prepared through the seed emulsion grafting co-polymerization methods. The structures of the increasing-compatible-matrix-stuff was analyzed by fourier transform infrared spectroscopy(FTIR) and nuclear magnetic resonate(NMR). The compellent damping materials was prepared using different ratio of the increasing-compatible-matrix-stuff, and the properties was tested with dynamic mechanic analysis(DMA) methods. The results show that the increasing-compatible-matrix-stuff has the special characteristics of compellent compatibility, and enhance the glass transformation temperature of the damping materials, and markedly develop the using-temperature-extent and the loss tangent of the damping materials.
文摘In situ ATR-FTIR was used to study the interaction of bovine serum albumin（BSA） and hydroxyapatite（HA） in this work.Both on the electrochemically deposited HA and self assemblied titanate nanotubes（TNT） substrates,the adsorption of BSA increases with the increase of time,and its adsorption on HA surface is much larger than that on TNT surface,implying that HA has a good biocompatibility as a biomaterial.It is indicated that Ca2+ plays an essential role in the protein absorption on the HA surface,due to that BSA is negatively charged,and it is able to combine with cation locations in HA particles by electrostatic forces.It is found that,when reacting with BSA,the phosphate band of HA in solution shifts to higher wavenumbers,indicating that PO3-4 can also be an adsorption site for BSA.The spectroscopic study on the interaction between HA and BSA will be helpful for further understanding the nano hydroxyapatite bioproperties on molecular level.
