Objective: To establish a mouse model of skin squamous cell carcinoma induced by high dose 7, 12-dimethyl-benzodiazepine [a] anthracene, and to provide experimental basis for the prevention and treatment of skin squam...Objective: To establish a mouse model of skin squamous cell carcinoma induced by high dose 7, 12-dimethyl-benzodiazepine [a] anthracene, and to provide experimental basis for the prevention and treatment of skin squamous cell carcinoma. Methods: 36 BALB/c mice were randomly divided into 3 groups, respectively is: the high dose group (group A), low dose group (group B), and blank control group (group c), recorded in the process of building body weight in mice, and skin changes back, local skin tissue for building after HE staining of PCNA immunohistochemical staining, and the expression of PCNA positive staining level as reflected in mice skin squamous cell carcinoma of the malignant indicators, using the Image Pro Plus calculation PCNA staining of integral optical density (IOD) and positive area, and the results were statistically analyzed. Results: at 12 weeks, there were statistically significant differences in tumor number and tumor volume between group A and group B (P<0.05), and the tumor bearing rate was up to 83.3% and 66.7%, and the incidence of SCC was up to 75% and 33.3%. Immunohistochemical analysis showed statistical differences in IOD and positive area of mice in groups A, B and C (P<0.01). Conclusion: compared with the mouse model of skin squamous cell carcinoma established by the traditional two-stage protocol, this model has the characteristics of shorter experimental period, higher tumor formation rate and easier operation, providing a reliable experimental basis for the study of skin squamous cell carcinoma.展开更多
A model of the universe (preprint 2019), based on a quantum approach to the evolution of space-time as well as on an equation of state that retains all the infinitesimal terms, has made it possible to estimate a large...A model of the universe (preprint 2019), based on a quantum approach to the evolution of space-time as well as on an equation of state that retains all the infinitesimal terms, has made it possible to estimate a large number of parameters relating to the universe and in particular the estimation of a colossal phantom energy E<sub>Λ</sub> represented by the existence of a hidden photon ŷpresent everywhere. This energy undergoes dilution in H<sup>4</sup> due to expansion of the universe. In order to introduce the effects of this energy on the curvature of space-time, we chose to express it by the cosmological constant Λ in the equation of the GR via the element tensor T<sup>00</sup>. This positive energy E<sub>Λ</sub> which acts as additional effect to gravity and we have expressed this energy in the form of an equation which expresses a so-called cosmological force F<sub>Λ</sub>. We estimated that this photon or hidden particle of spin 1 has an energy ~1 [meV] at our cosmic position t<sub>0</sub> which makes it an ultra-light axion ULA. Subsequently, with the action of this augmented force, especially in the first 400 [My] we were able to explain, in part, the rapid development of galaxy formation as seen by JWST as well as several observed dynamic behaviors of the barionic mass of some galaxies as MW, M33, UGC12591, NGC3198, UGC2885 and NGC253 whose observations raise questions and require additional explanations that led to the likely existence of unobserved matter called DM. However, it appears that this cosmological force makes it possible to explain several observations without the use of this DM. A first conclusion was drawn, namely the much earlier formation of galaxies by the action of this cosmological force coupled with gravity (GLASS z12). In addition, the model made it possible to explain the need or not to use the concept of DM for ETGs and LTGs by the more or less early and long period of the beginning of galaxy formation over a period ranging from ~170 to 1200 [My]. Thus, the model makes it possible to explain to a large extent the observations of the dynamics of the galaxies studied. However, several questions remain.展开更多
基金Beijing University of Chemical Technology-China-Japan Friendship Hospital Joint Fund (PYBZ1710)
文摘Objective: To establish a mouse model of skin squamous cell carcinoma induced by high dose 7, 12-dimethyl-benzodiazepine [a] anthracene, and to provide experimental basis for the prevention and treatment of skin squamous cell carcinoma. Methods: 36 BALB/c mice were randomly divided into 3 groups, respectively is: the high dose group (group A), low dose group (group B), and blank control group (group c), recorded in the process of building body weight in mice, and skin changes back, local skin tissue for building after HE staining of PCNA immunohistochemical staining, and the expression of PCNA positive staining level as reflected in mice skin squamous cell carcinoma of the malignant indicators, using the Image Pro Plus calculation PCNA staining of integral optical density (IOD) and positive area, and the results were statistically analyzed. Results: at 12 weeks, there were statistically significant differences in tumor number and tumor volume between group A and group B (P<0.05), and the tumor bearing rate was up to 83.3% and 66.7%, and the incidence of SCC was up to 75% and 33.3%. Immunohistochemical analysis showed statistical differences in IOD and positive area of mice in groups A, B and C (P<0.01). Conclusion: compared with the mouse model of skin squamous cell carcinoma established by the traditional two-stage protocol, this model has the characteristics of shorter experimental period, higher tumor formation rate and easier operation, providing a reliable experimental basis for the study of skin squamous cell carcinoma.
文摘A model of the universe (preprint 2019), based on a quantum approach to the evolution of space-time as well as on an equation of state that retains all the infinitesimal terms, has made it possible to estimate a large number of parameters relating to the universe and in particular the estimation of a colossal phantom energy E<sub>Λ</sub> represented by the existence of a hidden photon ŷpresent everywhere. This energy undergoes dilution in H<sup>4</sup> due to expansion of the universe. In order to introduce the effects of this energy on the curvature of space-time, we chose to express it by the cosmological constant Λ in the equation of the GR via the element tensor T<sup>00</sup>. This positive energy E<sub>Λ</sub> which acts as additional effect to gravity and we have expressed this energy in the form of an equation which expresses a so-called cosmological force F<sub>Λ</sub>. We estimated that this photon or hidden particle of spin 1 has an energy ~1 [meV] at our cosmic position t<sub>0</sub> which makes it an ultra-light axion ULA. Subsequently, with the action of this augmented force, especially in the first 400 [My] we were able to explain, in part, the rapid development of galaxy formation as seen by JWST as well as several observed dynamic behaviors of the barionic mass of some galaxies as MW, M33, UGC12591, NGC3198, UGC2885 and NGC253 whose observations raise questions and require additional explanations that led to the likely existence of unobserved matter called DM. However, it appears that this cosmological force makes it possible to explain several observations without the use of this DM. A first conclusion was drawn, namely the much earlier formation of galaxies by the action of this cosmological force coupled with gravity (GLASS z12). In addition, the model made it possible to explain the need or not to use the concept of DM for ETGs and LTGs by the more or less early and long period of the beginning of galaxy formation over a period ranging from ~170 to 1200 [My]. Thus, the model makes it possible to explain to a large extent the observations of the dynamics of the galaxies studied. However, several questions remain.
文摘采用HOCK的分布式能量物质平衡模型对老虎沟12号冰川消融期的物质平衡进行了模拟,时间步长为1 h,空间分辨率为30 m.模型结果利用物质平衡观测数据和气象站观测数据验证,模型模拟时期为2012年6月1日-9月30日.模型模拟结果表明,地形因子对太阳辐射影响相当显著;散射辐射在总辐射中的比例较大为39%,模拟期冰川表面物质平衡为-506 mm w.e..在模拟期整个冰川平均上净辐射占能量收入的84%,感热通量占有16%;消融耗热则是能量的主要支出占有62%,潜热通量占有能量支出的38%.
