The changes of CD4^＋CD25^＋ regulatory T cells （CD4^＋CD25^＋ Treg） and Foxp3 mRNA in peripheral blood mononuclear cells （PBMCs） from patients with asthma were investigated in order to elucidate the possible role...The changes of CD4^＋CD25^＋ regulatory T cells （CD4^＋CD25^＋ Treg） and Foxp3 mRNA in peripheral blood mononuclear cells （PBMCs） from patients with asthma were investigated in order to elucidate the possible roles of CD4^＋CD25^＋ Treg in the development of asthma. The peripheral blood samples were collected from 29 healthy controls （normal control group） and 78 patients with asthma which included 30 patients in exacerbation group, 25 patients in persistent group, and 23 patients in remission group. By using flow cytometry and RT-PCR, the CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA in PBMCs were detected. The CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA in PBMCs of exacerbation and persistent groups were lower than that of remission and normal control groups （P〈0.05）. Although the CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA of remission group were also lower than that of normal control group, there was no significant difference between them （P〉0.05）. As compared with persistent group, exacerbation group had lower CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA （P〈0.05）. It was indicated that the decrease of CD4^＋CD25^＋ Treg ratio and its function in PBMCs may be responsible for pathogenesis of asthma.展开更多
目的:研究五虎汤对病毒诱发幼年哮喘大鼠脾细胞悬液T-bet、GATA-3、RORγt、Foxp3 m RNA表达的影响。方法:采用RSV诱导OVA致敏大鼠哮喘模型。造模成功后,随机分成五虎汤高、中、低[4.752 g/(kg·d)、2.376 g/(kg·d)、1.188 g/(...目的:研究五虎汤对病毒诱发幼年哮喘大鼠脾细胞悬液T-bet、GATA-3、RORγt、Foxp3 m RNA表达的影响。方法:采用RSV诱导OVA致敏大鼠哮喘模型。造模成功后,随机分成五虎汤高、中、低[4.752 g/(kg·d)、2.376 g/(kg·d)、1.188 g/(kg·d)]剂量组、地塞米松组[地塞米松片,2.4 mg/(kg·d))]、模型组。干预14 d后,取脾组织,采用RT-PCR法检测脾细胞悬液中T-bet、GATA-3、RORγt、Foxp3 m RNA表达情况。结果:模型组脾组织T-bet、Foxp3m RNA表达低于空白组(P<0.05),而GATA-3、RORγt m RNA表达高于空白对照组(P<0.05)。五虎汤高、中剂量组脾组织GATA-3、RORγt m RNA表达均明显低于模型组(P<0.01或P<0.05),而T-bet、Foxp3 m RNA表达均明显高于模型组(P<0.01或P<0.05)。结论:五虎汤能调控病毒诱发幼年哮喘大鼠脾组织转录因子T-bet、GATA-3、RORγt、Foxp3 m RNA的表达,调控Thl、Th2、Thl7、Treg细胞,重建免疫平衡,抑制哮喘气道炎症形成。展开更多
目的:回顾分析宫颈癌患者外周血叉状头螺旋转录因子3(Foxp3)mRNA、鳞状细胞癌抗原(SCCA)等的水平变化,相关性及联合检测对诊断宫颈癌的价值。方法:92例宫颈癌患者(宫颈癌组)和87例体检健康女性(对照组)分别采用RT-PCR和化学发光法检测...目的:回顾分析宫颈癌患者外周血叉状头螺旋转录因子3(Foxp3)mRNA、鳞状细胞癌抗原(SCCA)等的水平变化,相关性及联合检测对诊断宫颈癌的价值。方法:92例宫颈癌患者(宫颈癌组)和87例体检健康女性(对照组)分别采用RT-PCR和化学发光法检测两组外周血Foxp3 mRNA和SCCA以及糖类抗原CA50、CA125水平;Pearson法分析Foxp3 mRNA与SCCA和CA50、CA125水平的相关性;绘制ROC曲线,分析Foxp3 mRNA联合SCCA检测对诊断宫颈癌的价值。结果:宫颈癌组Foxp3 mRNA、SCCA、CA50和CA125水平明显高于对照组(13.71±5.27 vs 2.09±1.51、13.07±1.82 vs 2.85±0.08ng/ml、49.2±13.29 vs 15.61±6.94IU/ml、51.72±15.26 vs 11.57±7.64U/ml,均P<0.01)。宫颈癌患者Foxp3mRNA相对表达水平与SCCA含量呈正相关(r=0.577,P<0.05),但与CA50和CA125无明显相关性(r=0.013和0.085,均P>0.05)。ROC曲线分析显示,Foxp3 mRNA联合SCCA诊断宫颈癌的曲线下面积(AUC)为0.911(95%CI:0.760—0.995),灵敏度为93.10%,特异度为84.70%,明显高于单检Foxp3mRNA或SCCA(均P<0.05)。结论:Foxp3 mRNA与SCCA联合检测可提高对宫颈癌的诊断效能。展开更多
Background and aims:Crohn’s disease(CD)and intestinal tuberculosis(ITB)are both chronic granulomatous conditions with similar phenotypic presentations.Hence,there is need for a biomarker to differentiate between both...Background and aims:Crohn’s disease(CD)and intestinal tuberculosis(ITB)are both chronic granulomatous conditions with similar phenotypic presentations.Hence,there is need for a biomarker to differentiate between both these two diseases.This study aimed at genome-wide gene expression analysis of colonic biopsies from confirmed cases of ITB and CD in comparison with controls.To evaluate the role of T regulatory cells,forkhead box P3(FOXP3)mRNA expression was quantified in serum as well as in colonic biopsies from patients with ITB and with the controls.Methods:Paired samples,including serum and colonic biopsies,were taken from 33 study subjects(CD,ITB and controls),and total RNA was extracted.Human whole genome gene expression microarray analysis was performed using the Illumina HumanWG-6 BeadChip Kit with six total RNA samples of the three groups in duplicates.Real-time PCR for FOXP3 mRNA expression was analyzed in serum samples and colonic biopsy samples(4-CD,5-ITB,4-controls).Results:In CD and ITB there was 1.5-fold upregulation of 92 and 382 genes and 1.5-fold downregulation of 91 and 256 genes,respectively.Peroxisome proliferators via the PPARc pathway were most significantly downregulated(P<0.005)in CD.Additionally,the IL4/5/6 signaling pathways and Toll-like receptor signaling pathway were identified as significantly differentially regulated(P<0.005)at>2-fold change.In ITB,the complement activation pathway,specifically the classical pathway,was the most significantly upregulated.FOXP3 mRNA expression was significantly elevated in colonic biopsies obtained from ITB patients as compared with CD cases(4.7062.21 vs 1.4860.31,P=0.016).Conclusions:FOXP3 mRNA expression in colonic mucosa could be a discriminatory marker between ITB and CD.Upregulation of the complement activation pathway in ITB suggests that pathogenetic mechanisms for ITB are similar to those of pulmonary tuberculosis.In CD,downregulation of PPARc was seen in colonic tissue,suggesting that restoration of PPARc-dependent anti-microbial barrier function may be a therapeutic target.展开更多
基金This project was supported by a program of Science Project of Hubei Province (No.2003AA301C10).
文摘The changes of CD4^＋CD25^＋ regulatory T cells （CD4^＋CD25^＋ Treg） and Foxp3 mRNA in peripheral blood mononuclear cells （PBMCs） from patients with asthma were investigated in order to elucidate the possible roles of CD4^＋CD25^＋ Treg in the development of asthma. The peripheral blood samples were collected from 29 healthy controls （normal control group） and 78 patients with asthma which included 30 patients in exacerbation group, 25 patients in persistent group, and 23 patients in remission group. By using flow cytometry and RT-PCR, the CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA in PBMCs were detected. The CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA in PBMCs of exacerbation and persistent groups were lower than that of remission and normal control groups （P〈0.05）. Although the CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA of remission group were also lower than that of normal control group, there was no significant difference between them （P〉0.05）. As compared with persistent group, exacerbation group had lower CD4^＋CD25^＋ Treg ratio and Foxp3 mRNA （P〈0.05）. It was indicated that the decrease of CD4^＋CD25^＋ Treg ratio and its function in PBMCs may be responsible for pathogenesis of asthma.
文摘目的:研究五虎汤对病毒诱发幼年哮喘大鼠脾细胞悬液T-bet、GATA-3、RORγt、Foxp3 m RNA表达的影响。方法:采用RSV诱导OVA致敏大鼠哮喘模型。造模成功后,随机分成五虎汤高、中、低[4.752 g/(kg·d)、2.376 g/(kg·d)、1.188 g/(kg·d)]剂量组、地塞米松组[地塞米松片,2.4 mg/(kg·d))]、模型组。干预14 d后,取脾组织,采用RT-PCR法检测脾细胞悬液中T-bet、GATA-3、RORγt、Foxp3 m RNA表达情况。结果:模型组脾组织T-bet、Foxp3m RNA表达低于空白组(P<0.05),而GATA-3、RORγt m RNA表达高于空白对照组(P<0.05)。五虎汤高、中剂量组脾组织GATA-3、RORγt m RNA表达均明显低于模型组(P<0.01或P<0.05),而T-bet、Foxp3 m RNA表达均明显高于模型组(P<0.01或P<0.05)。结论:五虎汤能调控病毒诱发幼年哮喘大鼠脾组织转录因子T-bet、GATA-3、RORγt、Foxp3 m RNA的表达,调控Thl、Th2、Thl7、Treg细胞,重建免疫平衡,抑制哮喘气道炎症形成。
文摘目的:回顾分析宫颈癌患者外周血叉状头螺旋转录因子3(Foxp3)mRNA、鳞状细胞癌抗原(SCCA)等的水平变化,相关性及联合检测对诊断宫颈癌的价值。方法:92例宫颈癌患者(宫颈癌组)和87例体检健康女性(对照组)分别采用RT-PCR和化学发光法检测两组外周血Foxp3 mRNA和SCCA以及糖类抗原CA50、CA125水平;Pearson法分析Foxp3 mRNA与SCCA和CA50、CA125水平的相关性;绘制ROC曲线,分析Foxp3 mRNA联合SCCA检测对诊断宫颈癌的价值。结果:宫颈癌组Foxp3 mRNA、SCCA、CA50和CA125水平明显高于对照组(13.71±5.27 vs 2.09±1.51、13.07±1.82 vs 2.85±0.08ng/ml、49.2±13.29 vs 15.61±6.94IU/ml、51.72±15.26 vs 11.57±7.64U/ml,均P<0.01)。宫颈癌患者Foxp3mRNA相对表达水平与SCCA含量呈正相关(r=0.577,P<0.05),但与CA50和CA125无明显相关性(r=0.013和0.085,均P>0.05)。ROC曲线分析显示,Foxp3 mRNA联合SCCA诊断宫颈癌的曲线下面积(AUC)为0.911(95%CI:0.760—0.995),灵敏度为93.10%,特异度为84.70%,明显高于单检Foxp3mRNA或SCCA(均P<0.05)。结论:Foxp3 mRNA与SCCA联合检测可提高对宫颈癌的诊断效能。
基金This project was undertaken under the‘FIST’scheme of Department of Science and Technology,Government of India.In addition,support was taken from ICMR Senior Research Fellowship granted to VM.
文摘Background and aims:Crohn’s disease(CD)and intestinal tuberculosis(ITB)are both chronic granulomatous conditions with similar phenotypic presentations.Hence,there is need for a biomarker to differentiate between both these two diseases.This study aimed at genome-wide gene expression analysis of colonic biopsies from confirmed cases of ITB and CD in comparison with controls.To evaluate the role of T regulatory cells,forkhead box P3(FOXP3)mRNA expression was quantified in serum as well as in colonic biopsies from patients with ITB and with the controls.Methods:Paired samples,including serum and colonic biopsies,were taken from 33 study subjects(CD,ITB and controls),and total RNA was extracted.Human whole genome gene expression microarray analysis was performed using the Illumina HumanWG-6 BeadChip Kit with six total RNA samples of the three groups in duplicates.Real-time PCR for FOXP3 mRNA expression was analyzed in serum samples and colonic biopsy samples(4-CD,5-ITB,4-controls).Results:In CD and ITB there was 1.5-fold upregulation of 92 and 382 genes and 1.5-fold downregulation of 91 and 256 genes,respectively.Peroxisome proliferators via the PPARc pathway were most significantly downregulated(P<0.005)in CD.Additionally,the IL4/5/6 signaling pathways and Toll-like receptor signaling pathway were identified as significantly differentially regulated(P<0.005)at>2-fold change.In ITB,the complement activation pathway,specifically the classical pathway,was the most significantly upregulated.FOXP3 mRNA expression was significantly elevated in colonic biopsies obtained from ITB patients as compared with CD cases(4.7062.21 vs 1.4860.31,P=0.016).Conclusions:FOXP3 mRNA expression in colonic mucosa could be a discriminatory marker between ITB and CD.Upregulation of the complement activation pathway in ITB suggests that pathogenetic mechanisms for ITB are similar to those of pulmonary tuberculosis.In CD,downregulation of PPARc was seen in colonic tissue,suggesting that restoration of PPARc-dependent anti-microbial barrier function may be a therapeutic target.
