[Objectives] To optimize the processing technology of Mongolian Artemisia frigida Willd. and observe the safety and effectiveness of processed products. [Methods] The orthogonal test was conducted with the content of ...[Objectives] To optimize the processing technology of Mongolian Artemisia frigida Willd. and observe the safety and effectiveness of processed products. [Methods] The orthogonal test was conducted with the content of chlorogenic acid( CGA) in processed products and quercetin as the main indicator and reference factors. The impact of frying temperature and time and medicinal material granularity were examined to determine the optimal process. The safety and effectiveness were evaluated through adopting acute toxicity test in mice,and the bleeding time was determined by tail docking experiments,the blood coagulation time was determined with orbital venous plexus,the total number of platelets was counted for the whole blood. [Results]When the product was flipped and fried at the temperature of 270 ℃ for 20 min,the best effect was achieved. From the variance analysis,it proved that the frying temperature and the time and granularity had no significant effect on the content of the two components monitored. After processing,Artemisia frigida Willd.,it can shorten the bleeding time of mice and significantly increase the platelet number. [Conclusions]Appropriate frying can improve the safety and hemostatic effect. Therefore,this study can provide reference for verifying the theory of use of traditional Mongolian Artemisia frigida Willd.展开更多
An investigation of the EtOAc-soluble fraction from the aerial parts ofArtemisiafrigida Willd.（A. frigida） led to the isolation of three new flavonoid glycosides together with three known compounds. Their structures...An investigation of the EtOAc-soluble fraction from the aerial parts ofArtemisiafrigida Willd.（A. frigida） led to the isolation of three new flavonoid glycosides together with three known compounds. Their structures were elucidated by spectral experiments. At the same time, high-performance liquid chromatographic（HPLC） method was used for the simultaneous determination of the six flavonoid glycosides from the aerial parts ofA. frigida. The separation by gradient elution was performed on a Hypersil ODS-2 column（250 mm×4.6 mm, 5 μm） at 30℃ with acetonitrile and water as the mobile phase, and monitored by absorbance at 276 nm. The parameters of linearity, precision, accuracy and specificity of the method were evaluated. The recovery of the method is 96.50%--98.01%, and linearity（r〉0.9992） was obtained for all the flavonoid glycosides. A high degree of specificity as well as repeatability and reproducibility（relative standard deviation values less than 2.0%） were also achieved. This assay was applied to the determination of six flavonoid glycosides in ten samples. The results indicate that the developed assay method was rapid, accurate, reliable and could be readily utilized as a quantitative analysis method for A. frigida.展开更多
Objective To establish an HPLC method for the determination of seven flavonoids from the aerial part of Artemisia frigida. Methods Hypersil ODS-2 (300 mm × 4.6 mm, 5 μm) column was used, with acetonitril-0.2% ph...Objective To establish an HPLC method for the determination of seven flavonoids from the aerial part of Artemisia frigida. Methods Hypersil ODS-2 (300 mm × 4.6 mm, 5 μm) column was used, with acetonitril-0.2% phosphoric acid (gradient elution) as a mobile phase, and the detection wavelength was at 283 nm with flow rate at 1 mL/min. Results All calibration curves showed good linear regression (r > 0.9990) within the tested range. All average recovery was more than 98.00% and RSD was less than 3.0% (n = 6). Conclusion The method is steady and with good repeatability, and could be used to determine the content of flavonoids in A. frigida from different areas.展开更多
基金Supported by Key Project of the Inner Mongolian Government(2013-006)
文摘[Objectives] To optimize the processing technology of Mongolian Artemisia frigida Willd. and observe the safety and effectiveness of processed products. [Methods] The orthogonal test was conducted with the content of chlorogenic acid( CGA) in processed products and quercetin as the main indicator and reference factors. The impact of frying temperature and time and medicinal material granularity were examined to determine the optimal process. The safety and effectiveness were evaluated through adopting acute toxicity test in mice,and the bleeding time was determined by tail docking experiments,the blood coagulation time was determined with orbital venous plexus,the total number of platelets was counted for the whole blood. [Results]When the product was flipped and fried at the temperature of 270 ℃ for 20 min,the best effect was achieved. From the variance analysis,it proved that the frying temperature and the time and granularity had no significant effect on the content of the two components monitored. After processing,Artemisia frigida Willd.,it can shorten the bleeding time of mice and significantly increase the platelet number. [Conclusions]Appropriate frying can improve the safety and hemostatic effect. Therefore,this study can provide reference for verifying the theory of use of traditional Mongolian Artemisia frigida Willd.
文摘An investigation of the EtOAc-soluble fraction from the aerial parts ofArtemisiafrigida Willd.（A. frigida） led to the isolation of three new flavonoid glycosides together with three known compounds. Their structures were elucidated by spectral experiments. At the same time, high-performance liquid chromatographic（HPLC） method was used for the simultaneous determination of the six flavonoid glycosides from the aerial parts ofA. frigida. The separation by gradient elution was performed on a Hypersil ODS-2 column（250 mm×4.6 mm, 5 μm） at 30℃ with acetonitrile and water as the mobile phase, and monitored by absorbance at 276 nm. The parameters of linearity, precision, accuracy and specificity of the method were evaluated. The recovery of the method is 96.50%--98.01%, and linearity（r〉0.9992） was obtained for all the flavonoid glycosides. A high degree of specificity as well as repeatability and reproducibility（relative standard deviation values less than 2.0%） were also achieved. This assay was applied to the determination of six flavonoid glycosides in ten samples. The results indicate that the developed assay method was rapid, accurate, reliable and could be readily utilized as a quantitative analysis method for A. frigida.
文摘Objective To establish an HPLC method for the determination of seven flavonoids from the aerial part of Artemisia frigida. Methods Hypersil ODS-2 (300 mm × 4.6 mm, 5 μm) column was used, with acetonitril-0.2% phosphoric acid (gradient elution) as a mobile phase, and the detection wavelength was at 283 nm with flow rate at 1 mL/min. Results All calibration curves showed good linear regression (r > 0.9990) within the tested range. All average recovery was more than 98.00% and RSD was less than 3.0% (n = 6). Conclusion The method is steady and with good repeatability, and could be used to determine the content of flavonoids in A. frigida from different areas.
