[ Objective] This study aimed to investigate the biochemical and genetic characteristics of F1 hybrids of Ctenopharyngodon idellus ♀× Elopichthys bambusa ♂ - [ Method ] By using vertical polyacrylamide gel elec...[ Objective] This study aimed to investigate the biochemical and genetic characteristics of F1 hybrids of Ctenopharyngodon idellus ♀× Elopichthys bambusa ♂ - [ Method ] By using vertical polyacrylamide gel electrophoresis technology, three isozymes （LDH, EST, MDH） in nine tissues （heart, brain, eyes, liver, kidney, spleen, fin, muscle, plasma） of F1 hybrids of C. ideUus and parents were compared. [Result] Three isozymes in F1 hybrids of ♀× E. bambusa ♀ were analyzed; differences in isozyme and proteins between F1 hybrids idellus ♀× E. bambusa ♀ exhibited varying degrees of tissue-specificity; isozymes and proteins in F, hybrids were signiticandy different from the parents. [ Conclusion] These nine differences could be used as indicators to identify F1 hybrids and parents.展开更多
[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic h...[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.展开更多
The plant pathogen Verticillium dahliae causes severe cotton losses in Uzbekistan. To create cotton varieties that are resistant to the more virulent races of V.dahliae we wanted to determine
The behavior of wheat-rye translocation chromosome and alien chromosome including Thinopyrum and Haynaldia chromosome at meiosis was investigated in two hybrids by fluorescence in situ hybridization (FISH). Misdivisio...The behavior of wheat-rye translocation chromosome and alien chromosome including Thinopyrum and Haynaldia chromosome at meiosis was investigated in two hybrids by fluorescence in situ hybridization (FISH). Misdivision of translocation chromosome at anaphase I and rye chromatin micronucleus at tetrad stage were observed, A plant with one normal 1BL/1RS translocation chromosome and one 1BL/1RS translocation chromosome deleted about 1/3 of rye chromosome arm in length was identified. One plant with wheat-Thinopyrum non-Robertson translocation chromosome was also detected in the F-2 population of Yi4212 x Yi4095. That could be the results of unequal misdivision of wheat-rye 1BL/1RS translocation chromosome and Thinopyrum chromosome during meiosis. No interaction between translocation chromosome and alien chromosome at meiosis was supported by the data of the distribution frequencies of translocation chromosome and Thinopyrum or Haynaldia chromosome in the progeny of two hybrids. The results may be useful to cultivate new germplasms with different length of rye 1R short arm and wheat-alien non-Robertson translocation tines under wheat background.展开更多
Objective] This study was conducted to investigate the effects of different hormone combinations and concentrations on cal us induction and pIant regeneration of F1 hybrid of Solanum torvum SW. ＆#215; Solanum melonge...Objective] This study was conducted to investigate the effects of different hormone combinations and concentrations on cal us induction and pIant regeneration of F1 hybrid of Solanum torvum SW. ＆#215; Solanum melongena L. [Method] Media suppIemented with different combinations and concentrations of hormones were pre-pared for anther cal us induction, proIiferation, differentiation and regeneration. [Re-sult] MS＋1.0 m/L 2, 4-D＋0.5 mg/L 6-BA was the optimum medium for anther cal us induction. MS＋1.0 mg/L KT＋0.1 mg/L NAA was the optimum medium for cal us proIiferation. MS＋2.0 mg/L 6-BA＋0.3 mg/L NAA was the optimum medium for in-duction of adventitious buds. MS＋0.3 mg/L IAA was the optimum medium for root-ing of adventitious buds. The highest rooting rate was onIy 37.5 because induction of rooting was a difficuIt work. [Conclusion] The resuIts wiI provide references for outcross fertiIity and genetic improvement of eggpIant.展开更多
＇Taisi N0.3＇ is an early mid-maturing towel gourd F1 hybrid developed by crossing Ps17-6-5-2-10-8 as female parent and Ps39-3-8-4-6-5 as male parent. The period from sowing to first harvest is about 75 days in sprin...＇Taisi N0.3＇ is an early mid-maturing towel gourd F1 hybrid developed by crossing Ps17-6-5-2-10-8 as female parent and Ps39-3-8-4-6-5 as male parent. The period from sowing to first harvest is about 75 days in spring cropping. It grows exuberantly in early stage and better in middle and later stages; leaves are green and palmately divided; the maximum leaf＇s length and width are about 25.5 and 33.7 cm. It bears fruit at the main and lateral branch. The first female flower sets on the 7^th-8^th node, and the fruit-setting rate is 72.8%. The fruit-setting rate is high. Its fruit is long round stick shape, 47.4 cm in length, and 3.75 cm in width, with smooth and green skin covered by dark green stripes, dense flesh. The fruit tastes good, with high Vc content. It is resistant to downy mildew, moderately resistant to CMV, and highly resistant to blight. The average fruit weight is about 282.3 g with the general yield of 82 500 kg/hm2.展开更多
基金Supported by Project of Project of Science and Technology of Wuhan City(2013021001010464)
文摘[ Objective] This study aimed to investigate the biochemical and genetic characteristics of F1 hybrids of Ctenopharyngodon idellus ♀× Elopichthys bambusa ♂ - [ Method ] By using vertical polyacrylamide gel electrophoresis technology, three isozymes （LDH, EST, MDH） in nine tissues （heart, brain, eyes, liver, kidney, spleen, fin, muscle, plasma） of F1 hybrids of C. ideUus and parents were compared. [Result] Three isozymes in F1 hybrids of ♀× E. bambusa ♀ were analyzed; differences in isozyme and proteins between F1 hybrids idellus ♀× E. bambusa ♀ exhibited varying degrees of tissue-specificity; isozymes and proteins in F, hybrids were signiticandy different from the parents. [ Conclusion] These nine differences could be used as indicators to identify F1 hybrids and parents.
文摘[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.
文摘The plant pathogen Verticillium dahliae causes severe cotton losses in Uzbekistan. To create cotton varieties that are resistant to the more virulent races of V.dahliae we wanted to determine
文摘The behavior of wheat-rye translocation chromosome and alien chromosome including Thinopyrum and Haynaldia chromosome at meiosis was investigated in two hybrids by fluorescence in situ hybridization (FISH). Misdivision of translocation chromosome at anaphase I and rye chromatin micronucleus at tetrad stage were observed, A plant with one normal 1BL/1RS translocation chromosome and one 1BL/1RS translocation chromosome deleted about 1/3 of rye chromosome arm in length was identified. One plant with wheat-Thinopyrum non-Robertson translocation chromosome was also detected in the F-2 population of Yi4212 x Yi4095. That could be the results of unequal misdivision of wheat-rye 1BL/1RS translocation chromosome and Thinopyrum chromosome during meiosis. No interaction between translocation chromosome and alien chromosome at meiosis was supported by the data of the distribution frequencies of translocation chromosome and Thinopyrum or Haynaldia chromosome in the progeny of two hybrids. The results may be useful to cultivate new germplasms with different length of rye 1R short arm and wheat-alien non-Robertson translocation tines under wheat background.
基金Supported by National Science and Technology Program for Rural Development of China during the 12thFive-Year Plan Period(2012AA100103007)National Agricultural Science and Technology Achievement Transformation Fund(2013GB2E100381)+1 种基金Earmarked Fund for China Agriculture Research System(nycytxgxcxtd-03-10)Science and Technology Program of Guangxi(14123006-35,14123004-3-5)~~
文摘Objective] This study was conducted to investigate the effects of different hormone combinations and concentrations on cal us induction and pIant regeneration of F1 hybrid of Solanum torvum SW. ＆#215; Solanum melongena L. [Method] Media suppIemented with different combinations and concentrations of hormones were pre-pared for anther cal us induction, proIiferation, differentiation and regeneration. [Re-sult] MS＋1.0 m/L 2, 4-D＋0.5 mg/L 6-BA was the optimum medium for anther cal us induction. MS＋1.0 mg/L KT＋0.1 mg/L NAA was the optimum medium for cal us proIiferation. MS＋2.0 mg/L 6-BA＋0.3 mg/L NAA was the optimum medium for in-duction of adventitious buds. MS＋0.3 mg/L IAA was the optimum medium for root-ing of adventitious buds. The highest rooting rate was onIy 37.5 because induction of rooting was a difficuIt work. [Conclusion] The resuIts wiI provide references for outcross fertiIity and genetic improvement of eggpIant.
基金Supported by Special Fund for Vegetable Breeding of Zhejiang Province(2012C12903-3-2)Taizhou Agricultural Scientific Research Program(121TG01)~~
文摘＇Taisi N0.3＇ is an early mid-maturing towel gourd F1 hybrid developed by crossing Ps17-6-5-2-10-8 as female parent and Ps39-3-8-4-6-5 as male parent. The period from sowing to first harvest is about 75 days in spring cropping. It grows exuberantly in early stage and better in middle and later stages; leaves are green and palmately divided; the maximum leaf＇s length and width are about 25.5 and 33.7 cm. It bears fruit at the main and lateral branch. The first female flower sets on the 7^th-8^th node, and the fruit-setting rate is 72.8%. The fruit-setting rate is high. Its fruit is long round stick shape, 47.4 cm in length, and 3.75 cm in width, with smooth and green skin covered by dark green stripes, dense flesh. The fruit tastes good, with high Vc content. It is resistant to downy mildew, moderately resistant to CMV, and highly resistant to blight. The average fruit weight is about 282.3 g with the general yield of 82 500 kg/hm2.
