AIM To study the expression of Fas and Bcl 2 proteins in BEL 7404 human hepatoma cells in order to analyze the possible relationship between cell growth regulation by alpha fetoprotein(AFP) and Fas/Bcl 2 prot...AIM To study the expression of Fas and Bcl 2 proteins in BEL 7404 human hepatoma cells in order to analyze the possible relationship between cell growth regulation by alpha fetoprotein(AFP) and Fas/Bcl 2 proteins. METHODS BEL 7404 human hepatoma cells were maintained in RPMI 1640 medium supplemented with 10% new born calf serum. Cells adhered to coverslips were used to detect Fas and Bcl 2 protein expression by the avidin biotin complex (ABC) immunocytochemical assay. RESULTS Immunocytochemical study showed that essentially all the BEL 7404 human hepatoma cells could express Fas and Bcl 2 proteins, although in various amounts. No positive staining for Fas and Bcl 2 proteins was observed when cells were incubated with non relevant sera to establish the specificity. CONCLUSION Fas apoptosis signals and Bcl 2 rescue/survival signals from apoptosis are expressed in BEL 7404 human hepatoma cells. The finding strongly implys that AFP mediated cell apoptosis and growth enhancement are potentially associated with Fas and Bcl 2 proteins present in those cells.展开更多
BAOKGROUND： Bcl-2 and Fas proteins are well known as anti-apoptotic and pro-apoptotic factors respectively. However, whether the anti-epileptic mechanism of low-frequency repetitive transcranial magnetic stimulation ...BAOKGROUND： Bcl-2 and Fas proteins are well known as anti-apoptotic and pro-apoptotic factors respectively. However, whether the anti-epileptic mechanism of low-frequency repetitive transcranial magnetic stimulation （rTMS） involves an anti-apoptotic effect via regulating Bcl-2 and Fas protein expression remains to be determined. OBJECTIVE： To verify the correlation between the anti-epileptic mechanism following pretreatment of low-frequency rTMS and anti-hippocampal apoptosis. DESIGN, TIME AND SETTING： A randomized controlled animal experiment was performed at Institute of Neurological Disorders, Affiliated Hospital of North Sichuan Medical College between September 2007 and March 2008. MATERIALS： Pilocarpine （053K13011） was provided by Sigma, USA; lithium was provided by Shanghai Biotechnology Co., Ltd., China; Dantec Maglite-r25 rTMS instrument was provided by Dundee, Denmark. METHODS： A total of 21 adult male Wistar rats were randomly divided into control （n = 6）, rTMS pretreatment （n = 9）, and sham-stimulation （n = 6） groups. The rTMS pretreatment group was pretreated with low-frequency rTMS （0.5 Hz, 75% threshold intensity, 20 times/bundle, and 5 bundles/day）, while the sham-stimulation group was sham-stimulated with a similar sound for 7 successive days to establish lithium-pilocarpine-induced epileptic state models. MAIN OUTCOME MEASURES： Epileptic stroke latency; neuronal morphology was observed using hematoxylin and eosin staining; mean positive-reactive cell number and mean absorbance of Bcl-2 and Fas protein in the hippocampal CA1 region was observed using immunohistochemistry. RESULTS： Epileptic latency in the rTMS pretreatment group was significantly enhanced （P 〈 0.01）, and a number of degenerated neurons were observed to be apoptotic. Bcl-2 protein expression increased at each time point, but Fas protein expression decreased （P 〈 0.01）. CONCLUSION： Low-frequency rTMS has an anti-epileptic effect, which may be via regulation of Bcl-2 and Fas protein expression in the hippocampal region.展开更多
观察川芎嗪对顺铂诱导的耳聋模型大鼠耳蜗毛细胞的抗凋亡作用,并探索其抗凋亡的部分作用机制。方法 120只SPF级Wistar分为4组,正常对照组;正常+川芎嗪组;模型对照组;模型加川芎嗪组。采用顺铂腹腔注射的方法诱导药物性耳聋大鼠模型,通...观察川芎嗪对顺铂诱导的耳聋模型大鼠耳蜗毛细胞的抗凋亡作用,并探索其抗凋亡的部分作用机制。方法 120只SPF级Wistar分为4组,正常对照组;正常+川芎嗪组;模型对照组;模型加川芎嗪组。采用顺铂腹腔注射的方法诱导药物性耳聋大鼠模型,通过川芎嗪腹腔注射对顺铂耳聋模型大鼠进行干预,采用听觉脑干诱发电位ABR阈值的检测及大鼠耳蜗基底膜铺片的方法对模型进行评价,并对各组大鼠耳蜗组织中Fas、Fas L和caspase-8 m RNA及蛋白表达水平进行检测。结果模型对照组大鼠ABR阈值明显高于正常对照组,川芎嗪具有一定的降低顺铂耳聋模型大鼠ABR阈值的作用;顺铂耳聋模型大鼠耳蜗毛细胞明显溶解缺失、排列紊乱等病理改变;模型对照组大鼠耳蜗组织中Fas、Fas L和caspase-8 m RNA及蛋白表达水均显著高于正常对照组,经川芎嗪腹腔注射干预后,三者表达水平得到了一定的控制。结论顺铂可诱导药物性耳聋大鼠模型,川芎嗪对耳聋模型大鼠具有一定的干预作用;顺铂腹腔注射可导致大鼠耳蜗组织中Fas、Fas L和caspase-8 m RNA及蛋白表达水平,而川芎嗪注射液可能是通过抑制Fas、Fas L和caspase-8 m RNA及蛋白的表达水平而实现其抗凋亡作用。展开更多
文摘AIM To study the expression of Fas and Bcl 2 proteins in BEL 7404 human hepatoma cells in order to analyze the possible relationship between cell growth regulation by alpha fetoprotein(AFP) and Fas/Bcl 2 proteins. METHODS BEL 7404 human hepatoma cells were maintained in RPMI 1640 medium supplemented with 10% new born calf serum. Cells adhered to coverslips were used to detect Fas and Bcl 2 protein expression by the avidin biotin complex (ABC) immunocytochemical assay. RESULTS Immunocytochemical study showed that essentially all the BEL 7404 human hepatoma cells could express Fas and Bcl 2 proteins, although in various amounts. No positive staining for Fas and Bcl 2 proteins was observed when cells were incubated with non relevant sera to establish the specificity. CONCLUSION Fas apoptosis signals and Bcl 2 rescue/survival signals from apoptosis are expressed in BEL 7404 human hepatoma cells. The finding strongly implys that AFP mediated cell apoptosis and growth enhancement are potentially associated with Fas and Bcl 2 proteins present in those cells.
基金Youth Foundation Project of Sichuan Province, No. 04ZQ026-010
文摘BAOKGROUND： Bcl-2 and Fas proteins are well known as anti-apoptotic and pro-apoptotic factors respectively. However, whether the anti-epileptic mechanism of low-frequency repetitive transcranial magnetic stimulation （rTMS） involves an anti-apoptotic effect via regulating Bcl-2 and Fas protein expression remains to be determined. OBJECTIVE： To verify the correlation between the anti-epileptic mechanism following pretreatment of low-frequency rTMS and anti-hippocampal apoptosis. DESIGN, TIME AND SETTING： A randomized controlled animal experiment was performed at Institute of Neurological Disorders, Affiliated Hospital of North Sichuan Medical College between September 2007 and March 2008. MATERIALS： Pilocarpine （053K13011） was provided by Sigma, USA; lithium was provided by Shanghai Biotechnology Co., Ltd., China; Dantec Maglite-r25 rTMS instrument was provided by Dundee, Denmark. METHODS： A total of 21 adult male Wistar rats were randomly divided into control （n = 6）, rTMS pretreatment （n = 9）, and sham-stimulation （n = 6） groups. The rTMS pretreatment group was pretreated with low-frequency rTMS （0.5 Hz, 75% threshold intensity, 20 times/bundle, and 5 bundles/day）, while the sham-stimulation group was sham-stimulated with a similar sound for 7 successive days to establish lithium-pilocarpine-induced epileptic state models. MAIN OUTCOME MEASURES： Epileptic stroke latency; neuronal morphology was observed using hematoxylin and eosin staining; mean positive-reactive cell number and mean absorbance of Bcl-2 and Fas protein in the hippocampal CA1 region was observed using immunohistochemistry. RESULTS： Epileptic latency in the rTMS pretreatment group was significantly enhanced （P 〈 0.01）, and a number of degenerated neurons were observed to be apoptotic. Bcl-2 protein expression increased at each time point, but Fas protein expression decreased （P 〈 0.01）. CONCLUSION： Low-frequency rTMS has an anti-epileptic effect, which may be via regulation of Bcl-2 and Fas protein expression in the hippocampal region.
文摘观察川芎嗪对顺铂诱导的耳聋模型大鼠耳蜗毛细胞的抗凋亡作用,并探索其抗凋亡的部分作用机制。方法 120只SPF级Wistar分为4组,正常对照组;正常+川芎嗪组;模型对照组;模型加川芎嗪组。采用顺铂腹腔注射的方法诱导药物性耳聋大鼠模型,通过川芎嗪腹腔注射对顺铂耳聋模型大鼠进行干预,采用听觉脑干诱发电位ABR阈值的检测及大鼠耳蜗基底膜铺片的方法对模型进行评价,并对各组大鼠耳蜗组织中Fas、Fas L和caspase-8 m RNA及蛋白表达水平进行检测。结果模型对照组大鼠ABR阈值明显高于正常对照组,川芎嗪具有一定的降低顺铂耳聋模型大鼠ABR阈值的作用;顺铂耳聋模型大鼠耳蜗毛细胞明显溶解缺失、排列紊乱等病理改变;模型对照组大鼠耳蜗组织中Fas、Fas L和caspase-8 m RNA及蛋白表达水均显著高于正常对照组,经川芎嗪腹腔注射干预后,三者表达水平得到了一定的控制。结论顺铂可诱导药物性耳聋大鼠模型,川芎嗪对耳聋模型大鼠具有一定的干预作用;顺铂腹腔注射可导致大鼠耳蜗组织中Fas、Fas L和caspase-8 m RNA及蛋白表达水平,而川芎嗪注射液可能是通过抑制Fas、Fas L和caspase-8 m RNA及蛋白的表达水平而实现其抗凋亡作用。
