A novel nano crystalline Ag2O2-PbO2 film chemically modified electrode (CME) was prepared and the CME was characterized by X-ray diffractometer (XRD) and atomic force microscope (AFM). By chronoamperometry, the nano A...A novel nano crystalline Ag2O2-PbO2 film chemically modified electrode (CME) was prepared and the CME was characterized by X-ray diffractometer (XRD) and atomic force microscope (AFM). By chronoamperometry, the nano Ag2O2-PbO2 CME was used as bioelectro- chemical sensor to determine the population of Escherichia coli (E. coli) in water. Compared with conventional methods, it is found that the technique we used is fast and convenient in counting E. coli.展开更多
[ Objective] Based on salicylic acid culture solution, high performance liquid chromatography （HPLC） was used to rapidly count E. coli in water. [Method] Because E. coli metabolism could produce polyatomic phenol （...[ Objective] Based on salicylic acid culture solution, high performance liquid chromatography （HPLC） was used to rapidly count E. coli in water. [Method] Because E. coli metabolism could produce polyatomic phenol （mainly 2, 5-dihydroxybenzoic acid） in salicylic acid culture solu- tion, and generated amount of polyatomic phenol was proportionate to the number of E. coli, we detected 2, 5-dihydroxybenzoic acid by HPLC. Through linear relationship, the number of E. co/i was calculated, realizing fast counting of E. coli, and detection time only needed 3 h. [ Result] Linearity range of the method detecting E. coli was 5 × 10^4 -5 × 10^10cells/ml, and the detection limit was 1 × 10^4 cells/ml. [ Conclusion] HPLC method realized rapid counting of E. coil Key words E. coli; Fast counting; HPLC; Salicylic acid; China展开更多
基金We are greateful to the National Narural Science Foundation of China(No.20455017)Science and Technology Committee of Shanghai Municipal(No.0452nm084).
文摘A novel nano crystalline Ag2O2-PbO2 film chemically modified electrode (CME) was prepared and the CME was characterized by X-ray diffractometer (XRD) and atomic force microscope (AFM). By chronoamperometry, the nano Ag2O2-PbO2 CME was used as bioelectro- chemical sensor to determine the population of Escherichia coli (E. coli) in water. Compared with conventional methods, it is found that the technique we used is fast and convenient in counting E. coli.
基金Supported by Plan Item of National Bureau of Quality Inspection,China(2011QK345)Natural Science Foundation Item of Guangxi,China(Guikezi0728048)
文摘[ Objective] Based on salicylic acid culture solution, high performance liquid chromatography （HPLC） was used to rapidly count E. coli in water. [Method] Because E. coli metabolism could produce polyatomic phenol （mainly 2, 5-dihydroxybenzoic acid） in salicylic acid culture solu- tion, and generated amount of polyatomic phenol was proportionate to the number of E. coli, we detected 2, 5-dihydroxybenzoic acid by HPLC. Through linear relationship, the number of E. co/i was calculated, realizing fast counting of E. coli, and detection time only needed 3 h. [ Result] Linearity range of the method detecting E. coli was 5 × 10^4 -5 × 10^10cells/ml, and the detection limit was 1 × 10^4 cells/ml. [ Conclusion] HPLC method realized rapid counting of E. coil Key words E. coli; Fast counting; HPLC; Salicylic acid; China
