Application of Luxol Fast Blue staining in locating the corticospinal tract in adult rats

BACKGROUND: There are many methods for myelin staining, mordant, or oil-soluble dye or the special reaction of osmic acid with lipoid is used according to different principles. The commonly used methods are classic Well staining, classic lithium carbonate-haematine staining, fast green staining, silver staining, etc. Luxol Fast Blue can brightly stain myelin sheath, and has certain specificity. The background can be very clean if there is proper differentiation, whereas Luxol Fast Blue is cheap and convenient to operate, thus it is an ideal staining reagent for routine myelin sheath. OBJECTIVE: To show the corticospinal tract of normal adult rats with Luxol Fast Blue staining method. DESIGN: A repetitive measurement design. SETTINGS: Institute of Nuerobiology, Nantong University; Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University. MATERIALS: Six healthy adult male SD rats of clean degree, weighing averagely 300 g, were provided by the experimental animal center of Nantong University. 1 g/L Luxol Fast Blue solution was provided by Sigma Company; Leica CM1900 cryostat microtome by Leica Company; Leica DMR microscope by Leica Company. METHODS: The experiment was carried out in the Staff Room of Human Anatomy, Nantong University in May 2005. The rats were given intraperitoneal injection of combined anesthetic (2 mL/kg), then the chest was open for perfusing saline and phosphate buffer containing formamint via heart. Brain and spinal cord were removed after 1 hour then fixed, then changed to phosphate buffer (pH 7.4) containing 300 g/L saccharu at 4 ℃, and stayed overnight, tissue blocks at pyramid, decussation of pyramid and cervical, thoracic, lumbar and sacral segments of spinal cord were removed to prepare continuous horizontal frozen sections (30 μm) after sedimentation, the sections were dried at room temperature. The corticospinal tract of normal adult rats were shown with Luxol Fast Blue staining method, and observed under Leica DMR microscope. MAIN OUTCOME MEASURES: Positive fibers in Luxol Fast Blue staining. RESULTS: After the Luxol Fast Blue staining, the labeled myelinated nerve fibers were bright blue. They located in the pyramid, decussation of pyramid and the ventral part of posterior funiculus in cervical, thoracic, lumbar and sacral segments of spinal cord. CONCLUSION: Luxol Fast Blue staining method may manifest the distribution of corticospinal tract with clear distinct in adult rats.

Analysis of the Initial Stage Intracloud Lightning Using the Pulse Location Technique Based on the Fast Electric Field Change

Academy of Sciences,Beijing 100029 2 Lanzhou University,Lanzhou 730000 3 Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences,Lanzhou 730000 4 Nanjing University of Information Science＆Technology,Nanjing 210044 Using both fast and slow electric field change sensors and field mill,multi-station observations on lightning flashes over China inland plateau areas were conducted during the summer of 2004.All of the stations were synchronized by GPS with a time-resolution of±50 ns.Using the different time of arrival（DTOA）and based on the fast electric field change sensor,a lightning radiation location technique was developed.Radiation pulses in the initial stages of five intracloud（IC）lightning discharges which occurred on 20 August were analyzed.The results indicate that the technique developed could effectively locate the lightning radiation sources.Furthermore,the lightning discharges were compared with the Doppler radar data.The results show that the radiation sources were well associated with the storm development.When the storm was at the mature stage with an echo top of about 10.9 km,the radiation sources ranged from 5.2 to 8.3 km above mean sea level;when the storm gradually became weaker,with echo top of about 7.9 km,the radiation sources ranged from 3.0 to 5.9 km.In particular,one of the IC lightning discharges ranged from 3.0 to 4.9 km during the dissipation stage of the storm.The results also indicate that the radiation sources were closely associated with the high reflectivity region（25–50 dBZ）of the storm,which,to some extent,demonstrates the reliability of the location results,thereby showing that multi-station observations of fast electric field change sensors could be a useful tool for monitoring the storm development.Location errors from radiation sources were also compared with the radar data and the results of a simple simulation.It was found that the errors were getting smaller when the radiation sources approached the center of the detection network, and vice versa.Compared with the limited experimental observations,the simulation results were found capable of effectively reflecting the location errors.