Experimental study of the effect of adipose stromal vascular fraction cells on the survival rate of fat transplantation

Objective To investigate the effect of adipose stromal vascular fraction cells(SVFs)on the survival rate of fat ransplantation.Methods 0.5mL autologous fat tissue was mixed with: ① DiI-labeled autologous SVFs (Group A);②

Clinical application of autologous chyle fat transplantation in the correction of sunken upper eyelid

Aim:We investigated the clinical application of autologous chyle fat in the correction of sunken upper eyelid.Methods:From November 2020 to October 2021,89 cases of correction with autologous chyle fat to sunken upper eyelid were performed(with or without other cosmetic procedures related to the eyes).An appropriate amount of fat was extracted from the superficial layer of the patient’s thigh and processed to chyle fat.About 0.5-2.8 mL of fat was injected under the orbicularis oculi muscle(roof-retro orbicularis oculi fat)or in the area where the orbital septal fat exists from the outer orbital margin of the upper eyelid.Results:In total,59 patients were followed up for 1-11 months after surgery.No infection,necrosis,vascular embolism,upper eyelid lumpiness,fat calcification,or liquefaction occurred in all patients,while seven cases showed inadequate correction.The depression basically disappeared,the shape was relatively full,the youthfulness was greatly improved,and the satisfaction of patients was high during the follow-up period.Conclusion:The correction with autologous chyle fat to sunken upper eyelid showed little trauma,short recovery periods,and satisfactory results,and it is worthy of being popularized and applied more widely.

Review on the clinical application of autologous fattransplantation in secondary lip malformation after cleft lip surgery

Congenital cleft lip is a common congenital defect.At present,surgical treatment is the only effective treatment for congenital cleft lip.Currently,there are many surgical methods for cleft lip.Many scholars continue to the improvement of surgical methods,and the immediate effect after surgery is significantly improved.However,despite the continuous improvement of surgical methods,lip and nose deformities are inevitably left after cleft lip.Because the lip occupies an important aesthetic position in the face,the restoration of secondary lip deformity after cleft lip surgery has important clinical significance.There are individual differences in secondary lip deformities in patients with cleft lip,and there are various methods of rehabilitation.There is no uniform treatment standard.This article mainly focuses on the current treatment progress of secondary lip deformities after cleft lip,especially autologous fat transplantation in secondary lip deformity after cleft lip is reviewed as follows.

Observation on the Clinical Effect of Breast Augmentation by Transplanting Concentrated Growth Factor and Autologous Fat

Objective To explore the effect of concentrated growth factor(CGF)combined with autologous fat transplantation in augmentation mammoplasty.Methods Clinical data of 28 female patients who underwent autologous fat by CGF mixed with purified fat between May 2015 and June 2018 were retrospectively analyzed.Clinical evaluation was performed to observe and measure the shape of the breast after breast augmentation,the increase in the value of the nipple plane measurement,and complications.Results All of 28 patients,after 12 months of follow-up,all patients no fat infection,hematoma,breast nodules and other complications.It showed that the contouring of breast were improved and more chubbiness than that before operation.The results were satisfied from patients.Conclusion Concentrated growth factor combined with autologous fat transplantation has a stable breast augmentation effect,beautiful breast shape,simple operation and small damage,which achieves breast rejuvenation.

Enrichment with Adipose-Derived Stem Cells Does Not Enhance Water-Jet Fat Graft Survival in the Breast—A Prospective Comparative Study

Objective: Although research increasingly underlines the theoretical importance of adipose-derived stem cells (ASC) for successful fat transplantation, we could not prove this notion in cosmetic breast augmentation. Stem-cell-enriched breast fat transplantation did not yield better results than grafting based on the WAL (water-jet assisted liposuction) technique in terms of volume persistence. To overcome inter-individual variation, intra-individual comparison was performed. Material and Methods: Four women underwent breast augmentation utilizing WAL fat transplantation with and without ex-vivo ASC isolation performed with the Multistation System. All patients underwent two fat transplantations for breast augmentation. In the first procedure, the left breast was augmented with stem-cell-enriched fat grafts and the right with plain WAL fat grafts. In the second surgery, the lipofilling was performed the other way round to exclude intra-individual differences in fat cell persistence. Results: MRI volumetry revealed a mean volume survival of 56% of the whole (watery) graft in the breasts with WAL only and 56% in the breasts with WAL and stem cell enrichment. While centrifugation of the WAL grafts demonstrated an average adipose content of 68%, the average volume survival of the adipose tissue itself was 83% in the breasts augmented with WAL only and 83% in those with WAL with stem cell enrichment. Conclusion: Breast augmentation by means of lipofilling using WAL alone is faster and more inexpensive, carrying a lower risk of contamination and offering an equal take rate. We do not see any advantage in stem-cell-enrichment in cosmetic fat transplantation to the breast.

Ultrasonic integrated backscatter in assessing liver steatosis before and after liver transplantation

BACKGROUND： Liver steatosis affects 20%-30% of adults.Because of the increasing gap between graft supplies and demands, livers with steatosis are frequently used in liver transplantation. But severely steatotic liver grafts are associated with a high risk of intraoperative and postoperative complications. Accurate assessment of fat content of donor livers and monitoring of the extent of steatosis in recipients are required for liver transplantation. The present study aimed to determine the correlation between liver echogenicity and fat content, and to evaluate the use of an ultrasonic integrated backscatter system（IBS） in the assessment of changes in fat content after liver transplantation.METHODS： Seventy-nine consecutive patients receiving liver grafts from living donors were evaluated in our center. Of these recipients, 67 survived for more than two years and were included in this study. Each liver graft was evaluated with IBS and ultrasound before operation and the fat content was estimated. The fat content of the grafts in the recipients was again assessed with ultrasound at 18 months after surgery.RESULTS： A correlation was detected between each graft＇s IBS value and its fat content（P=0.001）. The IBS value in fatty grafts with various degrees of steatosis was significantly decreased in 3（P=0.02）, 12, 15 and 18（P=0.001） months after orthotopic liver transplantation. The IBS value returned to normal in all patients in 18 months after liver transplantation.CONCLUSIONS： Decreased fat content in steatotic grafts can be observed in all recipients. Ultrasonic IBS is useful in determining the steatotic degree of grafts in donors as well as in monitoring the grafts after liver transplantation.

Rapid printing of 3D porous scaffolds for breast reconstruction

Prosthesis implantation and fat transplantation are common breast reconstructionmethods.In general,prosthesis implantation alone does not achieve a realistic enough appearance,and fat transplantation alone is difficult to achieve in the correct capacity.To date,no reports have focused on methods of combining fat with implanted prostheses for breast reconstruction.Using a newly designed bionic ink(i.e.,polyether F127 diacrylate(F127DA)&poly(ethylene glycol)diacrylate(PEGDA))and projection-based three-dimensional bioprinting(PBP),we report the development of a new method for printing porous prostheses.PEGDA was used to improve the printing precision of the prosthesis by increasing the gel point of F127DA and reducing the impact of external temperature.The compression modulus of the printed prosthesis was very close to that of prostheses currently used in clinical practice and to that of natural breasts.Finally,stromal vascular fraction gel(SVF-gel),a human fat extract,was injected into the pores of the synthesized prostheses to prepare a prosthesis mixed with adipose tissue.These were implanted subcutaneously in nude mice to observe their biological performance.After 14 and 28 days of observation,the prosthesis showed good biocompatibility,and adipose tissues grew well in and around the prosthesis.This result shows that a porous prosthesis containing pre-placed adipose tissues is a promising breast reconstruction material.

Clinical Application of Platelet-Rich Fibrin in Dermatology

Platelet-rich fibrin(PRF)is a second-generation platelet concentrate obtained from autologous blood.PRF is composed of abundant platelets,leucocytes,and a high concentration of various growth factors and fibrinogen.The composition and three-dimensional structure of PRF enable it to effectively make cells migrate and proliferate,playing an important role in tissue repair.Furthermore,the easy preparation and low cost of PRF make it a good treatment option.Numerous articles have been published about the application of PRF in clinical practice,however,the application of PRF in dermatology has not been comprehensively reviewed.The objective of this review article was to discuss various applications of PRF in dermatology,including healing chronic wounds,treating androgenic alopecia,skin rejuvenation,autologous fat transplantation,and treating vitiligo.PRF is a promising dermatologic treatment,but lacks a standardized protocol regarding its methods of attainment and use,which needs more investigations.