Simulating Responses of Rice Yield and Nitrogen Fates to Ground Cover Rice Production System under Different Types of Precipitation Years

The Ground Cover Rice Production System(GCRPS)has considerable potential for securing rice production in hilly areas.However,its impact on yields and nitrogen(N)fates remains uncertain under varying rainfall conditions.A two-year field experiment(2021–2022)was conducted in Ziyang,Sichuan Province,located in the hilly areas of Southwest China.The experiment included two cultivation methods:conventional flooding paddy(Paddy,W1)and GCRPS(W2).These methods were combined with three N management practices:N1(no-N fertilizer),N2(135 kg/hm^(2)urea as a base fertilizer in both W1 and W2),and N3(135 kg/hm^(2)urea with split application for W1 and 67.5 kg/hm^(2)urea and chicken manure separately for W2).The WHCNS(Soil Water Heat Carbon Nitrogen Simulator)model was calibrated and validated to simulate ponding water depth,soil water storage,soil mineral N content,leaf area index,aboveground dry matter,crop N uptake,and rice yield.Subsequently,this model was used to simulate the responses of rice yield and N fates to GCRPS under different types of precipitation years using meteorological data from 1980 to 2018.The results indicated that the WHCNS model performed well in simulating crop growth and N fates for both Paddy and GCRPS.Compared with Paddy,GCRPS reduced N leaching(35.1%–54.9%),ammonia volatilization(0.7%–13.6%),N runoff(71.1%–83.5%),denitrification(3.8%–6.7%),and total N loss(33.8%–56.9%)for all precipitation year types.However,GCRPS reduced crop N uptake and yield during wet years,while increasing crop N uptake and yield during dry and normal years.Fertilizer application reduced the stability and sustainability of rice yield in wet years,but increased the stability and sustainability of rice yield in dry and normal years.In conclusion,GCRPS is more suitable for normal and dry years in the study region,leading to increased rice yield and reduced N loss.

Effect of Transplanting Density on Rice Yield,Nitrogen Uptake and ^(15)N-fertilizer Fate

[Objective] The aim of this study was investigated the rice yield, nitrogen uptake and ^15-fertilizer fate at different transplanting density to provide scientific ba- sis for improving the yield of rice and applying reasonably fertilizer. [Method] A field experiment was carried out to study the effect of different transplanting density on rice yield, nitrogen （N） absorption, sources of N uptake by rice and the N balance in the plant-soil systems by using ^15-labelled urea. [Result] There were no significant differences in rice yields and total N uptakes by rice between treatments 30 cm × 30 cm and 40 cm × 40 cm, but the yield of rice and total N absorption in the two treatments were remarkably higher than those in 50 cm × 50 cm treatment. The amounts of total N uptake by rice were in the range of 112.3-162.7 kg/hm2 in the three transplanting densities. The result showed that about 1/3 of the total N uptake by rice was supplied by application fertilizer and the other 2/3 was obtained from the soil N pool. The ^15N-labelled urea absorbed by rice, residual in soil and lost accounted for 16.3%-26.1%, 17.0%-20.9% and 53.0%-66.7% of the total fertilizer, respectively. A great deal of ^15N-labelled urea was lost during the rice growing season. [Conclusion] Considering the rice yield and environmental protection, the transplanting density of 30 cm×30 cm was recommended in the hilly area of Sichuan basin in the southwest China.

沉默FATE/BJ-HCC-2基因的表达对肝癌细胞迁移及侵袭的影响

目的:研究FATE/BJ-HCC-2基因表达与肝癌细胞迁移及侵袭的关系。方法:体外合成FATE/BJ-HCC-2基因序列特异性小干扰RNA(siRNA),转染肝癌细胞系克隆株5B4,并设非特异性siRNA转染和空白对照组。采用RT-PCR及Western blot方法检测各组细胞中FATE/BJ-HCC-2 mRNA和蛋白的表达;通过Transwell小室模型检测各组细胞体外迁移及侵袭的能力;激光共聚焦显微镜下观察5B4及空质粒转染的对照细胞(Mock)的骨架结构。结果:各组细胞中FATE/BJ-HCC-2 mRNA和蛋白表达差异有统计学意义(F=15.321,P=0.020),特异性siRNA转染组细胞低于其他2组(P<0.05)。抑制FATE/BJ-HCC-2基因表达后,细胞体外迁移及侵袭的能力降低(F=6.171和10.109,P<0.05)。5B4细胞形态拉长,蝌蚪状,呈现迁移、游走的状态;微丝丰富,形态规则,平行贯穿于细胞全长,细胞膜周围有较多丝状伪足伸出;微管减少,分布不规则。结论:FATE/BJ-HCC-2基因表达促进肝癌细胞的迁移及侵袭。

Fate of ^(15)N Labeled Nitrate and Ammonium Salts Added to an Alpine Meadow in the Qinghai-Xizang Plateau, China

To understand the dynamics of added nitrogen (N) in alpine meadow and the role of alpine plants and soil microorganisms in the retention of deposited N, the fate of 15 N labeled nitrate and ammonium salts was determined in an alpine meadow for two months. Two weeks after 15 N application, total recovery of 15 N from NO - 3_ 15 N was 73.5% while it was 78% from NH + 4_ 15 N. More 15 N was recovered in plants than in soil organic matter or in microbial biomass, irrespective of forms of N added. After one month, 70.6% of added NO - 3_ 15 N and 57.4% of NH + 4_ 15 N were recovered in soils and plants. 15 N recovered in soil organic matter decreased greatly while that recovered in plants varied little, irrespective of the form N. Compared with the results of two weeks after 15 N application, more NO - 3_ 15 N than NH + 4_ 15 N was recovered in microbial biomass. Total recovery was 58.4% (six weeks) and 67% (eight weeks) from NO - 3_ 15 N, and 43.1% and 49% from NH + 4_ 15 N, respectively. Both plants and soil microorganism recovered more NO - 3_ 15 N than NH + 4_ 15 N. But plants recovered more 15 N than soil microorganisms. During the whole experiment plants retained more NO - 3_N and 15 N than soil microorganisms while 15 N recovered in inorganic N pool did not exceed 1% due to lower amount of inorganic N. This indicates that plants play more important roles in the retention of deposited N although microbial biomass can be an important sink for deposited N in early days after N application.

FATE/BJ-HCC-2基因转染对肿瘤细胞生物学行为的影响

目的:观察FATE/BJ-HCC-2基因对肿瘤细胞生物学行为的影响,探讨FATE/BJ-HCC-2与肿瘤进展之间的关系。方法:将FATE/BJ-HCC-2基因转染肝癌细胞系Bel-7402细胞,3H-TdR掺入法和Transwell实验对转染细胞的增殖和侵袭能力进行检测;PCR方法检测细胞内的骨桥蛋白(OPN)和整合素连接激酶(ILK)基因的表达水平。结果:细胞增殖实验结果显示FATE/BJ-HCC-2转染肿瘤细胞的3H-TdR掺入值显著高于Mock细胞的掺入值,统计学分析二者差别有显著性意义(P<0.01)。FATE/BJ-HCC-2基因表达促进肝癌细胞系Bel-7402细胞的增殖,并且这种增殖效应可被特异性抗-FATE/BJ-HCC-2抗体所阻断。Transwell实验显示,转染FATE/BJ-HCC-2的细胞,其穿膜数量明显比对照组多,差别有显著的统计学意义(P<0.01)。对肿瘤细胞转移相关的细胞信号分子进行检测发现,骨桥蛋白(OPN)和整合素连接激酶(ILK)基因的表达水平上调。结论:肿瘤细胞获得FATE/BJ-HCC-2基因表达后其增殖和侵袭能力增强,FATE/BJ-HCC-2基因表达可能与肿瘤细胞的转移有一定关系。提示CT抗原的表达不仅仅是肿瘤发生中的伴随产物,它可能在肿瘤的发生发展过程中起到一定作用。

FATE/BJ-HCC-2基因表达促进细胞增殖及肿瘤形成

FATE/BJ-HCC-2是本实验室鉴定的一个新的肿瘤-睾丸抗原基因,定位于Xq28染色体.为探讨FATE/BJ-HCC-2对细胞生物学行为的影响及裸鼠体内肿瘤形成能力的改变,分别采用脂质体转染真核表达载体及逆转录病毒感染两种方式,获得了FATE/BJ-HCC-2表达的Bel-7402单克隆细胞株及NIH-3T3细胞克隆,并通过RT-PCR和Western印迹在基因和蛋白水平鉴定了FATE/BJ-HCC-2表达情况.通过3H-TdR参入法,检测细胞的增殖能力;通过稀释铺板集落形成率测定和软琼脂集落形成实验,检测细胞的集落形成能力;通过裸鼠体内细胞注射成瘤,检测细胞成瘤性和肿瘤生长速度.结果表明,FATE/BJ-HCC-2基因表达能明显提高细胞体外增殖、克隆形成能力和成瘤性.提示其对肿瘤的发生发展起到促进作用.

Analysis on the Feminine Psychological Need in the Marriage from Susan's Tragic Fate

Doris Lessing is a famous British female writer.Her short fiction to room 19 depicts the heroine Susan Rawing's tragic fate.The article makes an analysis on the feminine psychological need in the marriage from four aspects:Spiritual Freedom,Financially independent,interpersonal communication,mutual faith.

Prediction of the environmental fate and aquatic ecological impact of nitrobenzene in the Songhua River using the modified AQUATOX model

An accidental discharge of nitrobenzene happened in November 2005 in the Songhua River,China.The AQUATOX model was modified and adapted to simulate the time-dependent nitrobenzene distribution in this multimedia aquatic system and its potential ecological impacts.Nitrobenzene concentrations in flowing water,sediment,and biota were predicted.Based on the initial concentrations of nitrobenzene observed in the field during the accidental discharge,that is,0.167-1.47 mg/L at different river segments, the predic...

Environmental cues determine the fate of astrocytes after spinal cord injury

Reactive astrogliosis occurs after central nervous system（CNS） injuries whereby resident astrocytes form rapid responses along a graded continuum. Following CNS lesions, na？ve astrocytes are converted into reactive astrocytes and eventually into scar-forming astrocytes that block axon regeneration and neural repair. It has been known for decades that scarring development and its related extracellular matrix molecules interfere with regeneration of injured axons after CNS injury, but the cellular and molecular mechanisms for controlling astrocytic scar formation and maintenance are not well known. Recent use of various genetic tools has made tremendous progress in better understanding genesis of reactive astrogliosis. Especially, the latest experiments demonstrate environment-dependent plasticity of reactive astrogliosis because reactive astrocytes isolated from injured spinal cord form scarring astrocytes when transplanted into injured spinal cord, but revert in retrograde to naive astrocytes when transplanted into naive spinal cord. The interactions between upregulated type I collagen and its receptor integrin β1 and the N-cadherin-mediated cell adhesion appear to play major roles for local astrogliosis around the lesion. This review centers on the environment-dependent plasticity of reactive astrogliosis after spinal cord injury and its potential as a therapeutic target.

MicroRNAs as novel regulators of stem cell fate

Mounting evidence in stem cell biology has shown that microRNAs(miRNAs) play a crucial role in cell fate specification, including stem cell self-renewal, lineagespecific differentiation, and somatic cell reprogramming.These functions are tightly regulated by specific gene expression patterns that involve miRNAs and transcription factors. To maintain stem cell pluripotency, specific miRNAs suppress transcription factors that promote differentiation, whereas to initiate differentiation, lineagespecific miRNAs are upregulated via the inhibition of transcription factors that promote self-renewal. Small molecules can be used in a similar manner as natural miRNAs, and a number of natural and synthetic small molecules have been isolated and developed to regulate stem cell fate. Using miRNAs as novel regulators of stem cell fate will provide insight into stem cell biology and aid in understanding the molecular mechanisms and crosstalk between miRNAs and stem cells.Ultimately, advances in the regulation of stem cell fate will contribute to the development of effective medical therapies for tissue repair and regeneration. This review summarizes the current insights into stem cell fate determination by miRNAs with a focus on stem cell self-renewal, differentiation, and reprogramming. Small molecules that control stem cell fate are also highlighted.

Fate of Urea Nitrogen Applied to Rape Grown on a Red Soil and Efficiency of Urea in Raising Rape Yield

Fate of urea nitrogen (N) applied to rape grown on a red soil was investigated by the ￣(15)N mass balancetechnique, and efficiency of urea and effect of nutrients balance in raising rape yield were investigated ina field plot experiment. One hundred and thirty-eight kg N/ha, 86 kg N/ha as basal dressing and 52 kgN/ha as top dressing, was applied with band application technique. The experiment was conducted in thesoutheast of China, near Yingtan City, Jianxi Province.Results from is￣(15)N mass balance study showed that when urea was applied as basal dressing the plantrecovery was 44.0% of the applied N for Theatment T (with application of N, P, K, B and lime). Plantrecoveries were 38.0%-40.5% for Treatments -K, -B, -lime and +RS (without application of K, B or limeas well as with additional rice straw compared with Theatment T), which were not significantly differentfrom Treatment T. In contrast, plant recovery was only 5.1% for Treatment-P (without application of P),indicating that P was the factor limiting N uptake by rape. However, N remaining in 0-0.30 m soil was highup to 71.6% for Theatment -P, while the corresponding data were 33.0%-42.6% for the other treatments.The total recovery of applied N (including plant recovery and N remaining in 0-0.60 m soil) was 91.5% forTreatment T when urea was applied as basal dressing, while almost all the applied N was recovered when ureawas applied as top dressing. It was suggested that N loss was greatly controlled by using band applicationmethod in this experiment.Results from the field plot experiment showed that N supply capacity of this red soil was very low, andthe efficiency of the applied N was quite high, 7.1 kg rape seed was increased by application of one kg N forTreatmentT. Nitrogen and phosphorus were the key factors limiting rape yield, and the yield was very lowwhen neither of them was applied. The yield in TreatmentK was significantly lower than that in TYeatmentT, with the former accounting for 77% of the later.

Small molecules for mesenchymal stem cell fate determination

Mesenchymal stem cells(MSCs)are adult stem cells harboring self-renewal and multilineage differentiation potential that are capable of differentiating into osteoblasts,adipocytes,or chondrocytes in vitro,and regulating the bone marrow microenvironment and adipose tissue remodeling in vivo.The process of fate determination is initiated by signaling molecules that drive MSCs into a specific lineage.Impairment of MSC fate determination leads to different bone and adipose tissue-related diseases,including aging,osteoporosis,and insulin resistance.Much progress has been made in recent years in discovering small molecules and their underlying mechanisms control the cell fate of MSCs both in vitro and in vivo.In this review,we summarize recent findings in applying small molecules to the trilineage commitment of MSCs,for instance,genistein,medicarpin,and icariin for the osteogenic cell fate commitment;isorhamnetin,risedronate,and arctigenin for pro-adipogenesis;and atractylenolides and dihydroartemisinin for chondrogenic fate determination.We highlight the underlying mechanisms,including direct regulation,epigenetic modification,and post-translational modification of signaling molecules in the AMPK,MAPK,Notch,PI3K/AKT,Hedgehog signaling pathways etc.and discuss the small molecules that are currently being studied in clinical trials.The target-based manipulation of lineage-specific commitment by small molecules offers substantial insights into bone marrow microenvironment regulation,adipose tissue homeostasis,and therapeutic strategies for MSC-related diseases.

Analytical methods for investigating in vivo fate of nanoliposomes:A review

Nanoliposomes are considered to be the most successful nanoparticle drug delivery system, but their fate in vivo has not been fully understood due to lack of reliable bioanalytical methods, which seriously limits the development of liposomal drugs. Hence, an overview of currently used bioanalytical methods is imperative to lay the groundwork for the need of developing a bioanalytical method for liposome measurements in vivo. Currently, major analytical methods for nanoliposomes measurement in vivo include fluorescence labeling, radiolabeling, magnetic resonance imaging（MRI）, mass spectrometry and computed tomography. In this review, these bioanalytical methods are summarized, and the advantages and disadvantages of each are discussed. We provide insights into the applicability and limitations of these analytical methods in the application of nanoliposomes measurement in vivo, and highlight the recent development of instrumental analysis techniques. The review is devoted to providing a comprehensive overview of the investigation of nanoliposomes design and associated fate in vivo, promoting the development of bioanalytical techniques for nanoliposomes measurement, and understanding the pharmacokinetic behavior, effectiveness and potential toxicity of nanoliposomes in vivo.

JAK/STAT signaling regulates tissue outgrowth and male germline stem cell fate in Drosophila

In multicellular organisms, biological activities are regulated by cell signaling. The various signal transduction path- ways regulate cell fate, proliferation, migration, and polarity. Miscoordination of the communicative signals will lead to disasters like cancer and other fatal diseases. The JAK/STAT signal transduction pathway is one of the pathways, which was first identified in vertebrates and is highly conserved throughout evolution. Studying the JAK/STAT signal transduc- tion pathway in Drosophila provides an excellent opportunity to understand the molecular mechanism of the cell regu- lation during development and tumor formation. In this review, we discuss the general overview of JAK/STAT signaling in Drosophila with respect to its functions in the eye development and stem cell fate determination.

Fate of Nitrogen from Organic and Inorganic Sources in Rice-Wheat Rotation Cropping System

The lower availability of N is one of the most important limiting factors impeding crop yield enhancement among the various factors that affect crop yield under the multiple-cropping agroecosystem in China.In this study,the recovery of a single application of 15N-labeled fertilizer or residues in rice-wheat cropping system was determined,in order to provide theoretical foundation for the nitrogen management in sustainable agricultural production.A continuous trace experiment was conducted for 15N microplots by using randomized block design with four treatments and four replications（T1 = 15N-labeled fertilizer with crop residue incorporation,T2 = 15N-labeled residues,T3 = 14N fertilizer to generate unlabeled crop residue,and T4 = 15Nlabeled fertilizer without crop residue incorporation）.Our results showed that,on average,17.17 and 12.01% of crop N was derived from N fertilizer and 15N-labeled residues,respectively during the first growing season,suggesting that approximately 82.83 or 87.99% of crop N was derived directly from soil N pool.There was a larger difference in the 15N recovery pattern in crop when N was applied as fertilizer or residues,i.e.,most of crop N derived from N fertilizer was absorbed in the first growing season（92.04%）,and the relevant value was 38.03% when 15N-labeled residues were applied.This implied that most of N fertilizer was recovered in the present cropping season,while a longer residue effect will be found for 15N-labeled residues.Thus,the average recovery of N fertilizer and N residue in the soil after the first growing season was 33.46 and 85.64%,respectively.The recovery of applied N in soil when N was applied as residues was significantly higher than that when N was applied as fertilizer.There was a larger difference in the total 15N recovery in plant and soil when N was applied as fertilizer or residues.By the end of the fifth or sixth cropping season,the total 15N recovery in plant and soil when N was applied as fertilizer or residues were estimated at 64.38 and 79.11%,respectively.On the contrary,there was little difference between the practices of residue incorporation and residue removal following the N fertilizer application.N fertilizer appeared to be more readily available to crops than residue-N,and residue-N replenished soil N pool,especially N in soil organic matter,much more than N fertilizer after six growing seasons.Therefore,residue-N is a better source for sustaining N content of soil organic matter.Thus,one possible management practice is to use both organic and inorganic N sources simultaneously to improve the use efficiency of N while protecting the sustainability of soil.

Soxllb regulates the migration and fate determination of Müller glia-derived progenitors during retina regeneration in zebrafish

The transcription factor Sox11 plays important roles in retinal neurogenesis during vertebrate eye development.However,its function in retina regeneration remains elusive.Here we report that Sox11 b,a zebrafish Sox11 homolog,regulates the migration and fate determination of Müller glia-derived progenitors(MGPCs)in an adult zebrafish model of mechanical retinal injury.Following a stab injury,the expression of Sox11 b was induced in proliferating MGPCs in the retina.Sox11 b knockdown did not affect MGPC formation at 4 days post-injury,although the nuclear morphology and subsequent radial migration of MGPCs were alte red.At 7 days post-injury,Sox11 b knockdown res ulted in an increased proportion of MGPCs in the inner retina and a decreased propo rtion of MGPCs in the outer nuclear layer,compared with controls.Furthermore,Sox11 b knockdown led to reduced photoreceptor regeneration,while it increased the numbe rs of newborn amacrines and retinal ganglion cells.Finally,quantitative polymerase chain reaction analysis revealed that Sox11 b regulated the expression of Notch signaling components in the retina,and Notch inhibition partially recapitulated the Sox11 b knockdown phenotype,indicating that Notch signaling functions downstream of Sox11 b.Our findings imply that Sox11 b plays key roles in MGPC migration and fate determination during retina regeneration in zebrafish,which may have critical im plications for future explorations of retinal repair in mammals.

Leaf litter ecological fate in the Schelde Estuary in Belgium

Two dominant species of Willow( Salix triandra )and Reed (Phragmites australis) along the Schelde Estuary(in Belgium)were selected in this research. The pigments of higher plant was used as biomarkers, the decomposition process of the two species were studied after they fall into the Schelde Estuary. After statistical analysis(Spearman rank order correlation, P <0 05), the results has shown the decomposition dynamics pattern of the pigments, and the willow showed different pattern in comparing with the reed, e.g. Chlorophyll a decomposition dynamics for willow is: y 1=12196 x 2 - 175895 x +1E+06+ k , R 2=0 5706 while for reed is: y 2=-37878 x 2+229782 x +734282+ k , R 2=0 9065 The precise time of the leaf litter spent in the water was also calculated as were less than 24 days, 24-37 days, longer than 37 days(willow)and less than 24 days, longer than 24 days(reed), the leaf litter fate of the two dominant species in the Schelde Estuary was also compared.

Eustacia——Victim of Fate——On Thomas Hardy's The Return of the Native

Owing to the influences of Greek’s view of fate, Dorset peasant’s fatalism and Schopenhauer’ s pessimistic philosophy, Hardy showed more or less a pessimistic tendency in his early writings and this tendency illustrated itself in his writing of Eustacia, heroine of The Return of the Native. He believed that Eustacia’ s tragedy resulted from the disagreement between her aspiration for a brilliant life outside Egdon Heath and the fate imposed on her by some mysterious force working at odds with her. This is what the paper attempts to prove.

Orchestrating stem cell fate: Novel tools for regenerative medicine

Mesenchymal stem cells are undifferentiated cells able to acquire different phenotypes under specific stimuli. In vitro manipulation of these cells is focused on understanding stem cell behavior, proliferation and pluripotency. Latest advances in the field of stem cells concern epigenetics and its role in maintaining self-renewal and differentiation capabilities. Chemical and physical stimuli can modulate cell commitment, acting on gene expression of Oct-4, Sox-2 and Nanog, the main stemness markers, and tissue-lineage specific genes. This activation or repression is related to the activity of chromatin-remodeling factors and epigenetic regulators, new targets of many cell therapies. The aim of this review is to afford a view of the current state of in vitro and in vivo stem cell applications, highlighting the strategies used to influence stem cell commitment for current and future cell therapies. Identifying the molecular mechanisms controlling stem cell fate could open up novel strategies for tissue repairing processes and other clinical applications.